scholarly journals Mesoporous Silicon Microparticles Enhance MHC Class I Cross-Antigen Presentation by Human Dendritic Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
A. Jiménez-Periáñez ◽  
B. Abos Gracia ◽  
J. López Relaño ◽  
C. M. Diez-Rivero ◽  
P. A. Reche ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
T Lymphocytes ◽  
Mhc Class I ◽  
Cytotoxic T Lymphocytes ◽  
Class I ◽  
Antigenic Peptides ◽  
Mesoporous Silicon ◽  
Human Dendritic Cells ◽  
Antigen Presenting ◽  
First Time

The mesoporous silicon microparticles (MSMPs) are excellent vehicles for releasing molecules inside the cell. The aim of this work was to use MSMPs to deliver viral specific MHC class I restricted epitopes into human antigen presenting cells (monocyte derived dendritic cells, MDDCs) to facilitate their capture, processing, and presentation to CD8+ (cytotoxic) T lymphocytes. We show for the first time that MSMPs vehiculation of antigenic peptides enhances their MHC class I presentation by human MDDCs to CD8 T lymphocytes.

Can one predict antigenic peptides for MHC class I-restricted cytotoxic T lymphocytes useful for vaccination?

Vaccine ◽  
1993 ◽  
Vol 11 (9) ◽  
pp. 974-978 ◽  
Author(s):  
Véronique Calin-Laurens ◽  
Marie-Claude Trescol-Biémont ◽  
Denis Gerlier ◽  
Chantal Rabourdin-Combe
Keyword(s):  
T Lymphocytes ◽  
Mhc Class I ◽  
Cytotoxic T Lymphocytes ◽  
Class I ◽  
Antigenic Peptides

Characterization of the MHC class I cross-presentation pathway for cell-associated antigens by human dendritic cells

Blood ◽  
2003 ◽  
Vol 102 (13) ◽  
pp. 4448-4455 ◽  
Author(s):  
Jean Francois Fonteneau ◽  
Daniel G. Kavanagh ◽  
Margareta Lirvall ◽  
Catherine Sanders ◽  
Timothy L. Cover ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Mhc Class I ◽  
Antigen Processing ◽  
Brefeldin A ◽  
Class I ◽  
Cross Presentation ◽  
Presentation Pathway ◽  
Human Dendritic Cells ◽  
Antigen Presenting

AbstractMajor histocompatibility complex (MHC) class I presentation of exogenous antigens is the mechanism enabling professional antigen-presenting cells (APCs) to induce CD8+ T-cell responses against viruses and tumors that do not have access to the classical MHC class I pathway. We have characterized the uptake, processing, and MHC class I cross-presentation by human dendritic cells (DCs) of cell-associated antigens derived from physiologically relevant sources, namely, vaccinia virus-infected apoptotic and necrotic cells. We show that cross-presentation is a rapid process, detectable within 2 to 4 hours after uptake of dead cells, and that proteolysis by cathepsin D in an acidic endosomal compartment is essential for cross-presentation. The presentation is abolished when the phagocytic or macropinocytic functions of the cells are inhibited and is dependent on transporter associated with antigen processing, sensitive to brefeldin A, and requires functional proteasomes. Altogether, these data suggest that antigens derived from apoptotic and necrotic cells require access to the cytosol to intersect with the conventional MHC class I pathway for presentation of cytosolic proteins. (Blood. 2003;102:4448-4455)

scholarly journals Detection of major histocompatibility complex class I-restricted, HIV- specific cytotoxic T lymphocytes in the blood of infected hemophiliacs

Blood ◽  
1989 ◽  
Vol 73 (7) ◽  
pp. 1909-1914 ◽  
Author(s):  
RA Koup ◽  
JL Sullivan ◽  
PH Levine ◽  
D Brettler ◽  
A Mahr ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Mhc Class I ◽  
Cytotoxic T Lymphocytes ◽  
Mononuclear Cells ◽  
Nk Cell ◽  
Class I ◽  
Gene Products ◽  
Major Histocompatibility ◽  
Peripheral Blood Mononuclear ◽  
Hiv 1

Abstract Major histocompatibility (MHC)-restricted, human immunodeficiency virus type one (HIV-1)-specific, cytotoxic T lymphocytes (CTLs) were detected in the peripheral blood mononuclear cells (PBMCs) of HIV-1-infected individuals. Using a system of autologous B and T lymphoblastoid cell lines infected with recombinant vaccinia vectors (VVs) expressing HIV-1 gene products, we were able to detect HIV-1-specific cytolytic responses in the PBMCs of 88% of HIV-1-seropositive hemophiliac patients in the absence of in vitro stimulation. These cytolytic responses were directed against both HIV-1 envelope and gag gene products. The responses were resistant to natural killer (NK) cell depletion and were inhibited by monoclonal antibodies (MoAbs) to the T cell receptor, CD8 surface antigens, and MHC class I antigens, suggesting a classical MHC class I restricted, virus-specific CTL response.

scholarly journals Long-Peptide Cross-Presentation by Human Dendritic Cells Occurs in Vacuoles by Peptide Exchange on Nascent MHC Class I Molecules

2016 ◽  
Vol 196 (4) ◽  
pp. 1711-1720 ◽  
Author(s):  
Wenbin Ma ◽  
Yi Zhang ◽  
Nathalie Vigneron ◽  
Vincent Stroobant ◽  
Kris Thielemans ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Mhc Class I ◽  
Class I ◽  
Cross Presentation ◽  
Peptide Exchange ◽  
Human Dendritic Cells ◽  
Mhc Class I Molecules
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