scholarly journals Composition and Antioxidant Activity of Geopropolis Collected byMelipona subnitida(Jandaíra) Bees

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Silvana Alves de Souza ◽  
Celso Amorim Camara ◽  
Eva Monica Sarmento da Silva ◽  
Tania Maria Sarmento Silva
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Spectral Data ◽  
2D Nmr ◽  
Stingless Bee ◽  
Esi Ms ◽  
Melipona Subnitida ◽  
Isolation And Characterization

An investigation of the geopropolis collected byMelipona subnitida(jandaíra) stingless bee led to the isolation and characterization of two phenylpropanoids, 6-O-p-coumaroyl-D-galactopyranose (1) and 6-O-cinnamoyl-1-O-p-coumaroyl-β-D-glucopyranose (2), and seven flavonoids, 7-O-methyl-naringenin (3), 7-O-methyl aromadendrin (4), 7,4′-di-O-methyl aromadendrin (5), 4′-O-methyl kaempferol (6), 3-O-methyl quercetin (7), 5-O-methyl aromadendrin (8), and 5-O-methyl kaempferol (9). The structure of the new phenylpropanoid (1) was established from IR, LC-ESI-MS, and NMR spectral data, including 2D NMR experiments. The extract and fractions demonstrated significant antioxidant activity in DPPH, ABTS, andβ-carotene/linoleic acid tests.

scholarly journals Biflavonoids from the Unripe Fruits of Clusia Paralicola and their Antioxidant Activity

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Rafaela Ferreira Oliveira ◽  
Celso Amorim Camara ◽  
Maria de Fátima Agra ◽  
Tania Maria Sarmento Silva
Keyword(s):  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Spectral Data ◽  
2D Nmr ◽  
Cd Spectra ◽  
Total Extract ◽  
Isolation And Characterization ◽  
Β Carotene ◽  
Unripe Fruits

Investigation of the green fruits of Clusia paralicola (Clusiaceae) led to the isolation and characterization of two 3,8″-biflavonoids, 2R, 3S, 2″R, 3″R-GB1-7″- O-β-glucoside (1) and 2R, 3S, 2″R, 3,8″-binaringenin-7″-O-β-glucoside (2), together with four known compounds: β-sitosterol, stigmasterol, β-amyrin, and epicatechin. The structures were established from the IR, LC-ESI-MS and NMR spectral data, including 2D-NMR experiments. The absolute configurations of 1 and 2 were determined by CD spectra. The total extract and the biflavonoids demonstrated significant antioxidant activity in DPPH, ABTS, and β-carotene/linoleic acid tests.

Undescribed C-Glycosylflavones from Corn Silk and Potential Anti-inflammatory Activity Evaluation of Isolates

Planta Medica ◽  
2021 ◽  
Author(s):  
Satish Sarfare ◽  
Shabana Iqrar Khan ◽  
fazila zulfiqar ◽  
Srivedavyasasri Radhakrishnan ◽  
Zulfiqar Ali ◽  
...  
Keyword(s):  
Spectral Data ◽  
Structure Elucidation ◽  
2D Nmr ◽  
Inflammatory Activity ◽  
Inos Activity ◽  
Corn Silk ◽  
Activity Evaluation ◽  
Isolation And Characterization ◽  
Phytochemical Investigation

Phytochemical investigation of corn silk resulted in isolation and characterization of four flavone C-glycosides, chrysoeriol 6-C-β-oliopyranosyl-7-O-β-D-glucopyranoside (1), 3′-methoxycassiaoccidentalin A (2), chrysoeriol 6-C-β-boivinopyranosyl-7-O-β-D-glucopyranoside (3), and ax-4"-OH-3′-methoxymaysin (4), a triterpenoid, friedelin (5), two sterols, (22E)-5,8-epidioxyergosta-6,22-dien-3β-ol (6) and 6β-hydroxystigmasta-4,22-diene-3-one (7), and a mixture of β-sitosterol and stigmasterol. Compounds 1 and 2 were previously undescribed. Structure elucidation of the isolated compounds was attained using spectral data including 1D and 2D NMR and HRESIMS. Compounds 1, 2, 5, and 6 inhibited iNOS activity in LPS-induced macrophages and decreased nitrite levels by 68.64+4.46, 65.67 + 6.47, 88.50 + 0.50, and 94.00 + 4.00 %, respectively, at 50 µM. Compound 5 also showed inhibition of NF-κB (51.00+1.50 %). Compounds 1 and 2 induced NAG-1 activity in chondrocytes by 1.80 + 0.05 and 2.00 + 0.13 fold, respectively. The extract of corn silk, however, did not exhibit inhibition of iNOS or NF-κB but induced NAG-1 by 1.80+ 0.51 fold.

scholarly journals Two New Acylated Sucroses From the Roots of Canna indica L. and Their Antioxidant Activity

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199172
Author(s):  
Luu The Anh ◽  
Nguyen Hieu ◽  
Do Thi Trang ◽  
Bui Huu Tai ◽  
Phan Van Kiem
Keyword(s):  
Antioxidant Activity ◽  
Spectral Data ◽  
Peroxyl Radical ◽  
Internal Standard ◽  
2D Nmr ◽  
Canna Indica ◽  
Esi Ms ◽  
Extensive Analysis

Two new acylated sucroses, (6- O-axetoxyl)- β-d-fructofuranosyl-(2→1)-(6- O-feruloyl- α- d-glucopyranoside (1) and (6- O-axetoxyl)- β-d-fructofuranosyl-(2→1)-(6- O-( E)- p-coumaroyl- α- d-glucopyranoside (2), and four known compounds, 2-(3′,4′-dihydroxyphenyl)-1-propanol-4′- O-[4′′′-hydroxy-3′′′,5′′′-dimethoxybenzoyl-(→6′′)- β-d-fructofuranoside (3), tryptophol glucoside (4), corchonoside C (5), and 2-hydroxy-5-(2-hydroxyethyl)phenyl β-d-fructofuranoside (6), were isolated from the roots of Canna indica L. (Cannaceae). Their structures were determined by extensive analysis of HR-ESI-MS, 1D, and 2D NMR spectral data, as well as by comparison of these with those reported in the literature. Antioxidant activity of compounds 1-6 were evaluated by peroxyl radical absorbance capacity assay. Compounds 1 and 3 neutralized high amounts of peroxyl radical. At a concentration of 1 µM, their ORACROO* values were 3.07 ± 0.15 and 4.27 ± 0.30, respectively, many times greater than that of trolox, which was used as an internal standard. At 10 µM, the peroxyl radical absorbance capacity of compounds 1 and 3 exhibited equivalents to 15.60 ± 0.22 and 24.91 ± 0.43 times the net protection by 1 µM of trolox.

scholarly journals Isolation and Characterization of Crotosparsamide, a New Cyclic Nonapeptide from Croton sparsiflorus

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Rashad Mehmood ◽  
Abdul Malik
Keyword(s):  
Spectroscopic Data ◽  
2D Nmr ◽  
Spectral Studies ◽  
Isolation And Characterization ◽  
First Time

Crotosparsamide (1), a new cyclic nonapeptide, has been isolated from the n-butanol soluble sub-fraction of Croton sparsiflorus along with p-hydroxy methylcinnamate and kaempferol, which are reported for the first time from this species. Their structures were determined by chemical and spectral studies including ESIMS, and 1D and 2D NMR spectroscopic data.

scholarly journals Secondary metabolites of Phlebopus species from Northern Thailand

2020 ◽  
Vol 19 (12) ◽  
pp. 1525-1536
Author(s):  
Boontiya Chuankid ◽  
Hedda Schrey ◽  
Benjarong Thongbai ◽  
Olivier Raspé ◽  
Norbert Arnold ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Tumor Cell Lines ◽  
Submerged Cultures ◽  
Esi Ms ◽  
Spectroscopic Analyses ◽  
New Compounds ◽  
Comprehensive Characterization

AbstractSubmerged cultures of the edible mushrooms Phlebopus portentosus and Phlebopus spongiosus were screened for their secondary metabolites by HPLC-UV/Vis and HR-LC-ESI-MS. Two new compounds, 9′-hydroxyphenyl pulvinone (1), containing an unusual pulvinone structure, and phlebopyron (2), together with the seven known pigments, atromentic acid (3), xerocomic acid (4), variegatic acid (5), methyl atromentate (6), methyl isoxerocomate (7), methyl variegatate (8), and variegatorubin (9) were isolated from the cultures. Their structures were assigned on the basis of extensive 1D/2D NMR spectroscopic analyses, as well as HR-ESI-MS, and HR-ESI-MS/MS measurements. Furthermore, the isolated compounds were evaluated for their antimicrobial and cytotoxic properties. 9′-hydroxyphenyl pulvinone (1), xerocomic acid (4), and methyl variegatate (8) exhibited weak to moderate cytotoxic activities against several tumor cell lines. The present paper provides a comprehensive characterization of pigments from the class of pulvinic acids that are present in the basidiomes of many edible bolete species.

Isolation and characterization of microsatellite markers from the stingless bee Nannotrigona testaceicornis

2009 ◽  
Vol 1 (1) ◽  
pp. 97-99 ◽  
Author(s):  
Eddy J. F. Oliveira ◽  
Geusa S. Freitas ◽  
Aline S. Fonseca ◽  
Adna C. B. Sousa ◽  
Tatiana Campos ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Stingless Bee ◽  
Isolation And Characterization ◽  
Nannotrigona Testaceicornis

scholarly journals Isolation and Characterization of Two New Antimicrobial Acids from Quercus incana (Bluejack Oak)

2018 ◽  
Vol 2018 ◽  
pp. 1-5
Author(s):  
Rizwana Sarwar ◽  
Umar Farooq ◽  
Sadia Naz ◽  
Nadia Riaz ◽  
Syed Majid Bukhari ◽  
...  
Keyword(s):  
Ethyl Acetate Fraction ◽  
Structural Formula ◽  
2D Nmr ◽  
Spectroscopic Technique ◽  
Pentanoic Acid ◽  
One Dimensional ◽  
Isolation And Characterization ◽  
Nmr Techniques ◽  
New Compounds

Two new compounds [1-2] were purified from ethyl acetate fraction of Quercus incana. The structure of these compounds is mainly established by using advanced spectroscopic technique such as UV, IR, one-dimensional (ID) and two-dimensional (2D) NMR techniques, and EI mass. The structural formula was deduced to be 4-hydroxydecanoic acid [1] and 4-hydroxy-3-(hydroxymethyl) pentanoic acid [2]. Both isolated compounds were tested for their antimicrobial potential and showed promising antifungal activity against Aspergillus niger and Aspergillus flavus.

Isolation and characterization of microsatellite loci in the stingless bee Melipona interrupta manaosensis (Apidae: Meliponini)

2009 ◽  
Vol 2 (1) ◽  
pp. 27-30 ◽  
Author(s):  
I. B. Francini ◽  
A. C. B. Sousa ◽  
D. A. Sforça ◽  
M. F. F. Costa-Pinto ◽  
T. Campos ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Stingless Bee ◽  
Isolation And Characterization
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