scholarly journals Expression of Glutathione Peroxidase and Glutathione Reductase and Level of Free Radical Processes under Toxic Hepatitis in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Igor Y. Iskusnykh ◽  
Tatyana N. Popova ◽  
Aleksander A. Agarkov ◽  
Miguel Â. A. Pinheiro de Carvalho ◽  
Stanislav G. Rjevskiy
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Toxic Hepatitis ◽  
Free Radical Oxidation ◽  
Inflammatory Cells ◽  
Maximum Increase ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Radical Processes

Correlation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.

scholarly journals Intensity of cardiac free-radicals processes and expression of glutathione peroxidase and glutathione reductase genes in rats with adrenaline

2012 ◽  
Vol 58 (5) ◽  
pp. 530-538 ◽  
Author(s):  
I.Y. Iskusnykh ◽  
T.N. Popova ◽  
O.S. Musharova
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Free Radical Oxidation ◽  
Pathological Process ◽  
Mrna Levels ◽  
Maximal Increase ◽  
Radical Oxidation ◽  
Polymerase Chain ◽  
Radical Processes

The correlation between changes in activities of glutathione peroxidase and glutathione reductase in heart of rats during development of adrenaline myocarditis and intensity of free radical processes estimated by biochemiluminesce parameters and the content of lipoperoxidation products was demonstrated. The maximal increase of glutathione peroxidase and glutathione reductase activities (in 1.8 and 1.4 times accordingly) was observed t 24 h after the development of the pathological process; this coincided with the maximum intensity of prosesses of free radical oxidation. Using combination of reverse transcriptions with real-time polymerase chain reaction the cardiac mRNA levels of glutathione peroxidase and glutathione reductase genes were determined during the development of adrenaline myocarditis in rats. Analysis of expression of glutathione peroxidase and glutathione reductase genes showed, that the level of this transcripts demonstrated 2,8- and 7,3- increase in rats with adrenaline myocarditis, respectively. Obviously, overexpression of these enzymes can increase the resistance of cardiomyocites to oxidative stress.

Adaptogenic correction of free radical brain damage in subchronic exposure to sodium fl uoride

2020 ◽  
pp. 381-386
Author(s):  
A. G. Zhukova ◽  
L. G. Gorokhova ◽  
A. S. Kazitskaya ◽  
T. K. Yadykina ◽  
N. N. Mikhailova ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Brain Tissue ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Subchronic Exposure ◽  
Antioxidant Defense Enzymes ◽  
Radical Processes ◽  
Complex Drug

Introduction. Fluorine compounds in small doses, but with prolonged exposure, cause various disorders in organs at the cellular and molecular levels. Activation of free-radical processes plays an important role in the damaging eff ect of fl uorides. Th erefore, one of the most eff ective ways to limit fl uorine-induced damage is to directly aff ect free-radical processes using herbal preparations with antioxidant properties.The aim of the study is to study the eff ect of a dihydroquercetin-based drug on the activity of free radical processes in brain tissue under subchronic exposure to sodium fl uoride (NaF).Materials and methods. Th e work was performed on white male laboratory rats weighing 200-250 g. Th e rats were divided into 3 groups: 1 — control; 2 — rats with chronic exposure to sodium fl uoride (NaF) for 9 weeks; 3 — rats receiving a NAF solution with simultaneous administration of a complex drug based on dihydroquercetin at a dose of 3 mg/kg in 1% starch gel for 3, 6 and 9 weeks. The activity of free radical oxidation and antioxidant defense enzymes — superoxide dismutase (SOD) and catalase-was determined in the cerebral cortex. Th e level of expression of hypoxia-induced transcription factor HIF — 1A and inducible forms of proteins HSP72 and HSP32 were determined in the cytosolic fraction of brain tissue.Results. In the early stages of subchronic fl uoride exposure (1-3 weeks), the expression of protective proteins HIF-1α, HSP72, HSP32 and catalase was shown in the rat cortex, as a result of which the activity of free-radical processes was maintained at the control level. An increase in the timing of fl uoride intake to 9 weeks led to a decrease in antioxidant protection and signifi cant activation of free radical oxidation in brain tissue. Daily administration of a complex drug with dihydroquercetin for 3, 6 and 9 weeks to rats with subchronic fl uoride exposure led to a decrease in the severity of pro- and antioxidant balance disorders in the cerebral cortex. At the same time, the greatest protective eff ect of dihydroquercetin with fl uoride exposure was manifested by the 9th week of its administration.Conclusions. When subchronic intake of fl uorides in the body, the drug based on dihydroquercetin has a neuroprotective eff ect, which is manifested by an increase in the activity of antioxidant enzymes of fr ee radical oxidation and catalase and the resistance of the cortex to induced fr ee radical oxidation.

scholarly journals The antioxidant status at patients with a thyreotoxicosis syndrome at the combined therapy with epifamin

2010 ◽  
Vol 56 (3) ◽  
pp. 397-403 ◽  
Author(s):  
S.S. Popov ◽  
A.N. Pashkov ◽  
V.I. Zoloedov ◽  
T.N. Popova ◽  
T.I. Rakhmanova
Keyword(s):  
Free Radical ◽  
Blood Serum ◽  
Catalase Activity ◽  
Combined Therapy ◽  
Free Radical Oxidation ◽  
Traditional Treatment ◽  
Group 13 ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Radical Processes

The work purpose was research of degree of free-radical processes intensity and catalase activity in blood serum of patients with a thyreotoxicosis syndrome at traditional treatment and the combined therapy with epifamin. Patients (n=25) have been divided into 2 groups: the first group of patients - 12 persons who are on traditional treatment (antithyreoid drugs, β-adrenoblockers); the second group - 13 persons, are padding to basic therapy received epifamin on 1 tablet (10 mg) 3 times a day 10-15 minutes prior to meal within 7 days. Patients with thyreotoxicosis syndrome had an intensification of free-radical oxidation that was traced at measurement of biochemiluminescence parameters in blood serum. At the combined therapy with epifamin less expressed intensity of free-radical processes, and also increasing of the general antioxidant activity of an organism that did not descend at traditional treatment was observed. At carrying out of standard therapy of thyreotoxicosis syndrome, including application of antithyreoid drugs and β-adrenoblockers, catalase activity decreased, that unfavorable affected antioxidant potential of an organism. At the combined therapy with epifamin level of catalase activity, on the contrary, increased, that could influence positively on antioxidant protection of an organism.

scholarly journals Вплив чотирихлористого вуглецю на стан вільнорадикальних процесів організму щурів та морфологічні зміни у товстій кишці

2017 ◽  
Author(s):  
A. M. Pryshlyak ◽  
S. I. Yavorska ◽  
Т. К. Holovatа ◽  
B. Y. Reminetskyy
Keyword(s):  
Free Radical ◽  
Visual Analysis ◽  
White Male ◽  
Free Radical Oxidation ◽  
Male Rats ◽  
Pure Substance ◽  
Diene Conjugates ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Radical Processes

Introduction. Increased admission of herbal compounds in organism of humans and animals can trigger the development of functional disorders and pathological changes of the digestive system and in particular the colon.The aim of the study – tо evaluate the state of free radical processes and morphological changes in the colon with toxic experimental animals exposed by carbon tetrachloride.Research Methods. The study was performed on 44 white male rats weighing 180–200 g which were divided into 4 groups: group 1 – control (almost healthy animals); group 2–4 – rats with simulated toxic lesion by CCl4. Intragastric CCl4 was administered in 50.0 % oil solution at a rate of 0.2 ml of pure substance per100 g of body weight. The intensity of free radical processes evaluated with the level of malondialdehyde and diene conjugates, determined using spectrophotometric techniques; antioxidant system – with the activity of catalase in blood. According to the conventional method histological preparations were made. For qualitative and morphometric analysis system of a visual analysis of images using camera Vision Color CCD program and Inter Video Win DVR UTHSCSA Image Tool were used.Results and Discussion. The results shows that animals carbon tetrachloride intoxication is accompanied by activation of free radical oxidation of lipids, as evidenced by the increase in blood serum of animal content as malondialdehyde (MDA) and diene conjugates (DC) throughout the experiment, with the maximum concentration observed in malondialdehyde 7 days after poisoning. Under the influence of CC14 catalase activity increases in serum at the second, seventh and fourteenth days of the experiment in 1.3, 1.2 and 2.4 times respectively. Morphometric techniques revealed a significant restructuring of the mucosa, submucosa, muscular layer and serous membrane of the colon, accompanied by severe vascular disorders of alterative, infiltrative processes and changes of morphometric parameters of the colon’s wall structures at tissue and organ levels.Conclusions. In the case of CCl4 damage, the processes of free radical oxidation take place, the accumulation of toxic products of lipid peroxidation in the blood and the weakening of the enzyme system of antioxidant protection occur. A significant structural and morphometric reconstruction of the mucosa, submucosa, muscular and serous membranes of the colon has been established.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

The influence of humus acids of peloids and its components on free radical processes

2018 ◽  
Vol 64 (5) ◽  
pp. 429-432 ◽  
Author(s):  
N.P. Avvakumova ◽  
F.H. Kamilov ◽  
A.V. Zhdanova ◽  
I.A. Men’shikova ◽  
Yu.V. Zhernov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Albino Rats ◽  
Redox Systems ◽  
Radical Oxidation ◽  
The Third ◽  
Glutathione Peroxidases ◽  
Humus Acids ◽  
Radical Processes

The effect of individual components of humic substances of peloid on free radical oxidation processes has been investigated under conditions of oxidative stress induced in albino rats. Biological activity of peloids was determined using such parameters as the general antioxidant activity, activity superoxide dismutases, catalases and glutathione peroxidases on the third and tenth day of the experiment. Results indicate that the state of oxidative stress can be corrected on the third day of the experiment. Humic acids restore not only normal physiological redox systems, but also increase the activity of antioxidant enzymes on the 10th day.

scholarly journals ACETAMINOPHEN EFFECT ON FREE RADICAL OXIDATION INDICES IN RATS WITH TYPE 2 DIABETES MELLITUS

2017 ◽  
Author(s):  
O. B. Furka ◽  
I. B. Ivanusa ◽  
M. M. Mykhalkiv ◽  
I. M. Klishch
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Free Radical ◽  
Liver Homogenate ◽  
Free Radical Oxidation ◽  
Maximum Increase ◽  
Radical Oxidation ◽  
Age Related

Background. Acetaminophen is a drug used to relieve pain syndrome. It is used both independently and in composition of combined drugs. Type 2 diabetes is an age-related disease that is associated with a violation of insulin synthesis by pancreas.Objective. The aim of the research was to study the effect of acetaminophen on major free radical oxidation indices of rats with type 2 diabetes mellitus in time dynamics.Methods. We conducted two series of experiments. The first series comprised rats with type 2 diabetes mellitus and acute acetaminophen toxic lesions. The second series involved rats with type 2 diabetes mellitus and acetaminophen administration at a dose of 55 mg/kg for the period of 7 days.Results. Administration of acetaminophen for rats with type 2 diabetes mellitus caused the increase in the content of malondialdehyde, diene and triene conjugates and Schiff bases in blood plasma and malondialdehyde, diene and triene conjugates in liver homogenate. The maximum increase in these indices was observed on the first day of the experiment. Gradually these indices decreased on the 3rd, 5th and 7th days of the experiment.Conclusions. Free radical oxidation increased in both series of the experiment. This process developed in rats with type 2 diabetes mellitus and acute acetaminophen toxic lesions more intensively, than in rats with type 2 diabetes mellitus and administration of acetaminophen at the highest therapeutic dose during 7 days.

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