scholarly journals Intensity of cardiac free-radicals processes and expression of glutathione peroxidase and glutathione reductase genes in rats with adrenaline

2012 ◽  
Vol 58 (5) ◽  
pp. 530-538 ◽  
Author(s):  
I.Y. Iskusnykh ◽  
T.N. Popova ◽  
O.S. Musharova
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Free Radical Oxidation ◽  
Pathological Process ◽  
Mrna Levels ◽  
Maximal Increase ◽  
Radical Oxidation ◽  
Polymerase Chain ◽  
Radical Processes

The correlation between changes in activities of glutathione peroxidase and glutathione reductase in heart of rats during development of adrenaline myocarditis and intensity of free radical processes estimated by biochemiluminesce parameters and the content of lipoperoxidation products was demonstrated. The maximal increase of glutathione peroxidase and glutathione reductase activities (in 1.8 and 1.4 times accordingly) was observed t 24 h after the development of the pathological process; this coincided with the maximum intensity of prosesses of free radical oxidation. Using combination of reverse transcriptions with real-time polymerase chain reaction the cardiac mRNA levels of glutathione peroxidase and glutathione reductase genes were determined during the development of adrenaline myocarditis in rats. Analysis of expression of glutathione peroxidase and glutathione reductase genes showed, that the level of this transcripts demonstrated 2,8- and 7,3- increase in rats with adrenaline myocarditis, respectively. Obviously, overexpression of these enzymes can increase the resistance of cardiomyocites to oxidative stress.

scholarly journals Expression of Glutathione Peroxidase and Glutathione Reductase and Level of Free Radical Processes under Toxic Hepatitis in Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Igor Y. Iskusnykh ◽  
Tatyana N. Popova ◽  
Aleksander A. Agarkov ◽  
Miguel Â. A. Pinheiro de Carvalho ◽  
Stanislav G. Rjevskiy
Keyword(s):  
Free Radical ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Toxic Hepatitis ◽  
Free Radical Oxidation ◽  
Inflammatory Cells ◽  
Maximum Increase ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Radical Processes

Correlation between intensity of free radical processes estimated by biochemiluminesce parameters, content of lipoperoxidation products, and changes of glutathione peroxidase (GP, EC 1.11.1.9) and glutathione reductase (GR, EC 1.6.4.2) activities at rats liver injury, after 12, 36, 70, 96, 110, and 125 hours & tetrachloromethane administration have been investigated. The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96 h after exposure to CCl4. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96 h after exposure to CCl4, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl4administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.

State of the enzyme link of antioxidant protection in boys with hypoandrogenism

2020 ◽  
Vol 40 (4) ◽  
pp. 32-36
Author(s):  
L. K. Parkhomenko ◽  
◽  
L. A. Strashok ◽  
S. I. Turchina ◽  
G. V. Kosovtsova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Free Radical Oxidation ◽  
Conjugated Dienes ◽  
Delayed Puberty ◽  
Control Group ◽  
Antioxidant Protection ◽  
Radical Oxidation

Recently, interest in the problem of free radical oxidation in biological membranes, which is directly related to both the normal functioning of cells and the occurrence, course and outcome of many pathological conditions, has increased again in clinical medicine. The aim was to determine the role and impact of antioxidant defense in boys with hypoandrogenism. The study involved 75 adolescents with hypoandrogenism aged 13–18 years, who underwent a complex of clinical and laboratory examinations. All patients were conducted complex of anthropometric research and determination of the degree of delayed puberty, laboratory and instrumental examination. Free radical oxidation was determined by the levels of malondialdehyde, conjugated dienes, carbonated proteins, superoxide dismutase and catalase in the serum, and restored glutathione and glutathione peroxidase in whole blood. Based on their determination, the coefficient of oxidative stress was calculated. Statistical processing of results was performed using parametric and nonparametric methods. The study of indicators of the free radical oxidation process found that adolescents with hypoandrogenism have multidirectional changes in the oxidation of proteins and lipids, namely: the level of conjugated dienes increases, the concentration of malondialdehyde remains at the level of the control group, and the level of carbonated proteins tends to decrease. As for the activity of antioxidant protection enzymes, a significant decrease in the level of glutathione peroxidase was detected, while the level of superoxide dismutase and catalase remained at the level of normative indicators. Oxidative stress accompanies and is one of the pathogenetic links in the formation or maintenance of the state of hypoandrogenism in boys. This requires the use of antioxidants, the complex of which must be selected individually.

Adaptogenic correction of free radical brain damage in subchronic exposure to sodium fl uoride

2020 ◽  
pp. 381-386
Author(s):  
A. G. Zhukova ◽  
L. G. Gorokhova ◽  
A. S. Kazitskaya ◽  
T. K. Yadykina ◽  
N. N. Mikhailova ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Free Radical ◽  
Brain Tissue ◽  
Free Radical Oxidation ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Subchronic Exposure ◽  
Antioxidant Defense Enzymes ◽  
Radical Processes ◽  
Complex Drug

Introduction. Fluorine compounds in small doses, but with prolonged exposure, cause various disorders in organs at the cellular and molecular levels. Activation of free-radical processes plays an important role in the damaging eff ect of fl uorides. Th erefore, one of the most eff ective ways to limit fl uorine-induced damage is to directly aff ect free-radical processes using herbal preparations with antioxidant properties.The aim of the study is to study the eff ect of a dihydroquercetin-based drug on the activity of free radical processes in brain tissue under subchronic exposure to sodium fl uoride (NaF).Materials and methods. Th e work was performed on white male laboratory rats weighing 200-250 g. Th e rats were divided into 3 groups: 1 — control; 2 — rats with chronic exposure to sodium fl uoride (NaF) for 9 weeks; 3 — rats receiving a NAF solution with simultaneous administration of a complex drug based on dihydroquercetin at a dose of 3 mg/kg in 1% starch gel for 3, 6 and 9 weeks. The activity of free radical oxidation and antioxidant defense enzymes — superoxide dismutase (SOD) and catalase-was determined in the cerebral cortex. Th e level of expression of hypoxia-induced transcription factor HIF — 1A and inducible forms of proteins HSP72 and HSP32 were determined in the cytosolic fraction of brain tissue.Results. In the early stages of subchronic fl uoride exposure (1-3 weeks), the expression of protective proteins HIF-1α, HSP72, HSP32 and catalase was shown in the rat cortex, as a result of which the activity of free-radical processes was maintained at the control level. An increase in the timing of fl uoride intake to 9 weeks led to a decrease in antioxidant protection and signifi cant activation of free radical oxidation in brain tissue. Daily administration of a complex drug with dihydroquercetin for 3, 6 and 9 weeks to rats with subchronic fl uoride exposure led to a decrease in the severity of pro- and antioxidant balance disorders in the cerebral cortex. At the same time, the greatest protective eff ect of dihydroquercetin with fl uoride exposure was manifested by the 9th week of its administration.Conclusions. When subchronic intake of fl uorides in the body, the drug based on dihydroquercetin has a neuroprotective eff ect, which is manifested by an increase in the activity of antioxidant enzymes of fr ee radical oxidation and catalase and the resistance of the cortex to induced fr ee radical oxidation.

The influence of humus acids of peloids and its components on free radical processes

2018 ◽  
Vol 64 (5) ◽  
pp. 429-432 ◽  
Author(s):  
N.P. Avvakumova ◽  
F.H. Kamilov ◽  
A.V. Zhdanova ◽  
I.A. Men’shikova ◽  
Yu.V. Zhernov ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Albino Rats ◽  
Redox Systems ◽  
Radical Oxidation ◽  
The Third ◽  
Glutathione Peroxidases ◽  
Humus Acids ◽  
Radical Processes

The effect of individual components of humic substances of peloid on free radical oxidation processes has been investigated under conditions of oxidative stress induced in albino rats. Biological activity of peloids was determined using such parameters as the general antioxidant activity, activity superoxide dismutases, catalases and glutathione peroxidases on the third and tenth day of the experiment. Results indicate that the state of oxidative stress can be corrected on the third day of the experiment. Humic acids restore not only normal physiological redox systems, but also increase the activity of antioxidant enzymes on the 10th day.

Perspective Treatment Goals for Brain Protection in Case of Acute Ischemia

2019 ◽  
pp. 9-13
Author(s):  
L. A. Dzyak ◽  
O. S. Tsurkalenko ◽  
V. M. Suk
Keyword(s):  
Ischemic Stroke ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Reperfusion Therapy ◽  
Free Radical Scavenger ◽  
Acute Ischemia ◽  
Radical Scavenger ◽  
Important Place ◽  
Radical Oxidation ◽  
Radical Processes

Abstract. Ischemic stroke initiates a cascade of biochemical reactions, among which the processes of free radical oxidation occupy a key place. The most important place in management this disease takes reperfusion therapy, the conduct of which is closely related to the problem of neuroprotection. Use of antioxidants is a promising direction for the treatment of ischemic stroke. They have antioxidant effects and could prevent free radical processes. Edaravone is a new low molecular weight free radical scavenger, which inhibits the ischemic cascade. The article provides an overview of studies on the effectiveness and safety of edaravone in patients with ischemic stroke.

scholarly journals The state of free-radical oxidation and antioxidant system at the experimental acute peritonitis associated with hypothyroidism

2017 ◽  
Author(s):  
R. V. Verba ◽  
I. M. Klishch
Keyword(s):  
Free Radical ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Mononuclear Leukocytes ◽  
Lipid Hydroperoxides ◽  
Active Forms Of Oxygen ◽  
Radical Oxidation ◽  
Acute Peritonitis ◽  
Activity Of Enzymes ◽  
Radical Processes

To study the influence of hypothyroidism on the course of free-radical processes and state of antioxidant system in rats with acute extensive peritonitis, the production of active forms of oxygen by mononuclear leukocytes, concentration of lipid hydroperoxides, TBA-active products, Schiff bases, SOD and catalase activity were determined. Simulation of extensive peritonitis with associated hypothyroidism led to the less obvious, than in euthyroid rats, increase of active forms of oxygen and lipid peroxidation products. Although the reduction of activity of enzymes of the first line of antioxidant defense (superoxide dismutase and catalase) was more evident.

scholarly journals Characteristics of influence of 4-[4-oxo-3 h-quinazoline-3-yl] benzoic acid (pc-66) on a course of adrenaline myocardiodystrophy in rats according to biochemical research

2017 ◽  
Author(s):  
O. V. Dzhyhaliuk ◽  
H. I. Stepaniuk ◽  
N. V. Zaichko ◽  
S. I. Kovalenko ◽  
K. P. Shabelnyk
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Glutathione Peroxidase ◽  
Benzoic Acid ◽  
Free Radical Oxidation ◽  
Control Group ◽  
No Production ◽  
Marker Enzymes ◽  
Radical Oxidation ◽  
Group I

According to the literature the leading cause of mortality among the population of Ukraine are cardiovascular diseases, what incidence tripled over the past 25 years and today stands at801.6 in100 000. It necessitates a search for new chemical compounds, suitable for creating manufacturing with a better safety profile and a high degree of efficiency.The aim of the study was to describe the effect of 4-[4-oxo-4N-quinazoline-3- il] benzoic acid (PC-66), compared with kordaron on the processes of free radical oxidation, endothelial nitric oxide production and activity of marker enzymes cytolysis cardiomyocytes adrenaline myocardiodystrophy (AMD) in rats.The study was conducted on 28 nonlinear rats of both sexes (weight – 165–220 grams), divided into four groups: group I (n = 7) – intact rats; group II (n = 7) – rats with untreated AMD (control); group III (n = 7) – rats with AMD treated with entity PC-66 (10 mg/kg intraperitoneally (i/p)); Group IV (n = 7) – rats with AMD treated with kordaron (10 mg/kg, i/p).Research shown that the development of a pilot AMD in rats was characterized by increased free radical oxidation processes, depletion of antioxidant defense system (with decreased content of G-SH, activity of superoxide dismutase and glutathione peroxidase), oppression of endothelial NO production. The most expressive metabolic disturbances were registered in the acute period of AMD (as of 2nd day). The use of 4-[4-oxo-4N-quinazoline-3-il] benzoic acid (PC-66, 10 mg/kg, i/p) provided an expressive cardioprotective effect which was compared with kordaron (10 mg/kg, i/p) at various stages of AMD.The mechanisms of cardioprotective action 4-[4-oxo-4N-quinazoline-3-il] benzoic acid can be realized due to the stabilization of cellular and subcellular membranes (as evidenced by the dynamics of marker enzymes cytolysis cardiomyocytes in serum and reduction of TBA-reactive substances in the myocardium), increasing the efficiency of the antioxidant system and correction of cellular redox buffer (as evidenced by the increasing pool of reduced glutathione, glutathione peroxidase and superoxide dismutase activity increased) and anti-ischemic activity associated with increased activity of endothelial NO synthase in cardiac muscle.

scholarly journals The antioxidant status at patients with a thyreotoxicosis syndrome at the combined therapy with epifamin

2010 ◽  
Vol 56 (3) ◽  
pp. 397-403 ◽  
Author(s):  
S.S. Popov ◽  
A.N. Pashkov ◽  
V.I. Zoloedov ◽  
T.N. Popova ◽  
T.I. Rakhmanova
Keyword(s):  
Free Radical ◽  
Blood Serum ◽  
Catalase Activity ◽  
Combined Therapy ◽  
Free Radical Oxidation ◽  
Traditional Treatment ◽  
Group 13 ◽  
Radical Oxidation ◽  
Free Radical Processes ◽  
Radical Processes

The work purpose was research of degree of free-radical processes intensity and catalase activity in blood serum of patients with a thyreotoxicosis syndrome at traditional treatment and the combined therapy with epifamin. Patients (n=25) have been divided into 2 groups: the first group of patients - 12 persons who are on traditional treatment (antithyreoid drugs, β-adrenoblockers); the second group - 13 persons, are padding to basic therapy received epifamin on 1 tablet (10 mg) 3 times a day 10-15 minutes prior to meal within 7 days. Patients with thyreotoxicosis syndrome had an intensification of free-radical oxidation that was traced at measurement of biochemiluminescence parameters in blood serum. At the combined therapy with epifamin less expressed intensity of free-radical processes, and also increasing of the general antioxidant activity of an organism that did not descend at traditional treatment was observed. At carrying out of standard therapy of thyreotoxicosis syndrome, including application of antithyreoid drugs and β-adrenoblockers, catalase activity decreased, that unfavorable affected antioxidant potential of an organism. At the combined therapy with epifamin level of catalase activity, on the contrary, increased, that could influence positively on antioxidant protection of an organism.

scholarly journals Intensity of free radical processes in cartilage of rats with collagen-induced arthritis

2016 ◽  
Vol 21 (2) ◽  
pp. 27-30
Author(s):  
E. Tihova ◽  
O. Bernyk ◽  
O. Savchuk ◽  
K. Dvorshchenko ◽  
L. Ostapchenko
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Free Radical ◽  
Experimental Model ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Free Radical Oxidation ◽  
Collagen Induced Arthritis ◽  
Radical Oxidation ◽  
Radical Processes

Products of free radical oxidation and antioxidant enzymes in rats with collagen-induced arthritis were studied. It was established, that under experimental model of arthritis in rat cartilage the content of superoxide anion radical, peroxide and TBA-active compounds and the activity of antiradical enzymes superoxide dismutase and catalase increased.

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