Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects againstBordetella pertussisInfection

This study examined the immunogenic properties of the fusion protein fimbria 2 ofBordetella pertussis(Fim2)—cholera toxin B subunit (CTB) in the intranasal murine model of infection. To this endB. pertussisFim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence. The expression and assembly of the fusion protein into pentameric structures (CTB-Fim2) were evaluated by SDS-PAGE and monosialotetrahexosylgaglioside (GM1-ganglioside) enzyme-linked immunosorbent assay (ELISA). To evaluate the protective capacity of CTB-Fim2, an intraperitoneal or intranasal mouse immunization schedule was performed with 50 μg of CTB-Fim2. Recombinant (rFim2) or purified (BpFim2) Fim2, CTB, and phosphate-buffered saline (PBS) were used as controls. The results showed that mice immunized with BpFim2 or CTB-Fim2 intraperitoneally or intranasally presented a significant reduction in bacterial lung counts compared to control groups (P<0.01orP<0.001,resp.). Moreover, intranasal immunization with CTB-Fim2 induced significant levels of Fim2-specific IgG in serum and bronchoalveolar lavage (BAL) and Fim2-specific IgA in BAL. Analysis of IgG isotypes and cytokines mRNA levels showed that CTB-Fim2 results in a mixed Th1/Th2 (T-helper) response. The data presented here provide support for CTB-Fim2 as a promising recombinant antigen againstBordetella pertussisinfection.

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Expression of cholera toxin B subunit and the B chain of human insulin as a fusion protein in transgenic tobacco plants

Plant Cell Reports ◽

10.1007/s00299-005-0069-2 ◽

2005 ◽

Vol 25 (5) ◽

pp. 417-424 ◽

Cited By ~ 31

Author(s):

Dora Li ◽

Jennifer O’Leary ◽

Yan Huang ◽

Norman P. A. Huner ◽

Anthony M. Jevnikar ◽

...

Keyword(s):

Fusion Protein ◽

Cholera Toxin ◽

Transgenic Tobacco ◽

Human Insulin ◽

Cholera Toxin B Subunit ◽

Transgenic Tobacco Plants ◽

Toxin B ◽

Tobacco Plants ◽

Cholera Toxin B ◽

B Subunit

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Suppression of diabetes in non-obese diabetic (NOD) mice by oral administration of a cholera toxin B subunit–insulin B chain fusion protein vaccine produced in silkworm

Vaccine ◽

10.1016/j.vaccine.2006.10.039 ◽

2007 ◽

Vol 25 (8) ◽

pp. 1444-1451 ◽

Cited By ~ 23

Author(s):

Zhaohui Gong ◽

Yongfeng Jin ◽

Yaozhou Zhang

Keyword(s):

Fusion Protein ◽

Oral Administration ◽

Cholera Toxin ◽

Nod Mice ◽

Cholera Toxin B Subunit ◽

Protein Vaccine ◽

Toxin B ◽

Cholera Toxin B ◽

B Subunit

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Recombinant fusion protein of cholera toxin B subunit with YVAD secreted by Lactobacillus casei inhibits lipopolysaccharide-induced caspase-1 activation and subsequent IL-1 beta secretion in Caco-2 cells

BMC Biotechnology ◽

10.1186/1472-6750-14-38 ◽

2014 ◽

Vol 14 (1) ◽

pp. 38 ◽

Cited By ~ 2

Author(s):

Yukihiro Hiramatsu ◽

Masatatsu Yamamoto ◽

Tomomitsu Satho ◽

Keiichi Irie ◽

Akiko Kai ◽

...

Keyword(s):

Fusion Protein ◽

Cholera Toxin ◽

Lactobacillus Casei ◽

Recombinant Fusion Protein ◽

Cholera Toxin B Subunit ◽

Toxin B ◽

Cholera Toxin B ◽

B Subunit ◽

Caspase 1

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Modification of the cholera toxin B subunit coding sequence to enhance expression in plants

Molecular Breeding ◽

10.1023/b:molb.0000018762.27841.7a ◽

2004 ◽

Vol 13 (2) ◽

pp. 143-153 ◽

Cited By ~ 29

Author(s):

Tae-Jin Kang ◽

Nguyen-Hoang Loc ◽

Mi-Ok Jang ◽

Moon-Sik Yang

Keyword(s):

Cholera Toxin ◽

Cholera Toxin B Subunit ◽

Toxin B ◽

Coding Sequence ◽

Cholera Toxin B ◽

B Subunit

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Intranasal Immunization with the Cholera Toxin B Subunit-Pneumococcal Surface Antigen A Fusion Protein Induces Protection against Colonization with Streptococcus pneumoniae and Has Negligible Impact on the Nasopharyngeal and Oral Microbiota of Mice

Infection and Immunity ◽

10.1128/iai.00134-06 ◽

2006 ◽

Vol 74 (8) ◽

pp. 4939-4944 ◽

Cited By ~ 30

Author(s):

F. C. Pimenta ◽

E. N. Miyaji ◽

A. P. M. Arêas ◽

M. L. S. Oliveira ◽

A. L. S. S. de Andrade ◽

...

Keyword(s):

Streptococcus Pneumoniae ◽

Fusion Protein ◽

Cholera Toxin ◽

Surface Antigen ◽

Cholera Toxin B Subunit ◽

Toxin B ◽

Intranasal Immunization ◽

Cholera Toxin B ◽

B Subunit ◽

Antigen A

ABSTRACT One of the candidate proteins for a mucosal vaccine antigen against Streptococcus pneumoniae is PsaA (pneumococcal surface antigen A). Vaccines targeting mucosal immunity may raise concerns as to possible alterations in the normal microbiota, especially in the case of PsaA, which was shown to have homologs with elevated sequence identity in other viridans group streptococci. In this work, we demonstrate that intranasal immunization with a cholera toxin B subunit-PsaA fusion protein is able to protect mice against colonization with S. pneumoniae but does not significantly alter the natural oral or nasopharyngeal microbiota of mice.

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