scholarly journals Molecular Characterization ofCandida africanain Genital Specimens in Shanghai, China

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Yang Hu ◽  
Aihua Yu ◽  
Xiangming Chen ◽  
Guojiang Wang ◽  
Xiaobo Feng
Keyword(s):  
Candida Albicans ◽  
Sexually Transmitted Diseases ◽  
Amphotericin B ◽  
Molecular Characterization ◽  
Vaginal Swab ◽  
Vulvovaginal Candidiasis ◽  
Sexually Transmitted ◽  
Candida Africana

Candida africana, an emerging yeast pathogen, is closely related toCandida albicansand most commonly involved in vulvovaginal candidiasis (VVC). However, its prevalence in candidal balanoposthitis is still unclear. In this study, the prevalence ofC. africanain both candidal balanoposthitis and VVC in a sexually transmitted diseases (STD) clinic in Shanghai, China, was analyzed, and the molecular characterization and susceptible profiles ofC. africanaisolates were investigated. As results,C. africanawas only isolated in 5 out of 79 (6.3%) cases of candidal balanoposthitis rather than cases with vulvovaginal candidiasis. Among them, 4 out of 5 isolates share the same genotype of DST 782 with an isolate from vaginal swab in Japan published previously. AllC. africanaisolates were susceptible to amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole, posaconazole, caspofungin, and micafungin.

Vaginal candidosis (moniliasis)

1976 ◽  
Vol 14 (19) ◽  
pp. 75-76
Keyword(s):  
General Practice ◽  
Candida Albicans ◽  
Sexually Transmitted Diseases ◽  
Genital Tract ◽  
Immunosuppressive Drugs ◽  
Host Factors ◽  
Age Group ◽  
Sexually Transmitted ◽  
Younger Age ◽  
Vaginal Candidosis

Candida albicans is now the commonest cause of genital symptoms in women attending clinics for sexually transmitted diseases,1 and seems to be increasing in frequency.2 Host factors, including pregnancy, diabetes, obesity, the recent use of antibiotics, oral contraceptives, corticosteroids, or immunosuppressive drugs encourage the growth of the yeast in the genital tract. Both gonorrhoea and trichomoniasis have been reported to be commonly associated with candidosis in women attending the clinics for sexually transmitted diseases, especially in the younger age group. This is not true for the majority of women seen in general practice.3

scholarly journals 618. Fluconazole-Resistant Candida albicans Vaginitis with Cross-Resistance to Azoles: A Case Report

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S287-S288
Author(s):  
Damla Akdağ ◽  
Hüsnü Pullukçu ◽  
Tansu Yamazhan ◽  
Dilek Yesim Metin ◽  
Oğuz Reşat Sipahi ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Case Report ◽  
Clinical Response ◽  
Antifungal Susceptibility ◽  
Vaginal Swab ◽  
Vulvovaginal Candidiasis ◽  
Vaginal Candidiasis ◽  
Cross Resistance ◽  
High Dose

Abstract Background Local and systemic use of azole derivatives are common in the treatment of vulvovaginal candidiasis. However, there are cases unresponsive to these agents. Herein, we present treatment and follow-up of a patient with fluconazole–itraconazole and voriconazole-resistant recurrent vaginal candidiasis. Methods A 37-year-old woman with no comorbidity used topical and oral antifungal/antibacterial medications (including fluconazole and itraconazole) in the treatment of recurrent vulvovaginitis, was hospitalized due to continuous complaints. Intense, white-colored, odorless vaginal discharge was observed on physical examination. Urine and vaginal swab samples were taken for mycological and bacteriological culture. Metronidazole (500 mg 3x1 i.v.) and high dose fluconazole (600 mg/day i.v.) were initiated empirically for the possibility of dose-dependent resistant Candida infection, but there was no clinical response. Results Candida albicans was isolated in vaginal swab culture, but response to systemic fluconazole treatment for one week was inadequate. Antifungal susceptibility test was performed by microdilution method according to CLSI M27A3 guidelines and MIC values were reported respectively; fluconazole 4 µg/mL (SDD), itraconazole 1 µg/mL (R), posaconazole 0.06 µg/mL (WT), voriconazole 0.25 µg/mL (SDD), anidulafungin ≤ 0.015 µg/mL (S), amphotericin B 0.06 µg/mL (WT). For the resistance mechanism, point mutation in the ERG11 gene and MDR1 and MDR2 from efflux pumps were investigated and only the G464S mutation was detected in the ERG11 gene. Treatment was switched to IV anidulafungin (200 mg on day 1 followed by 100 mg/day). Clinical response was achieved in the patient whose complaints were reduced, and there was no Candida in the repeated vaginal swab culture taken on day 3 of treatment. The patient was discharged after 2 weeks of treatment. She had no recurrence after 2 years follow-up. Conclusion It should be kept in mind that resistant strains may be responsible for recurrent and unresponsive vulvovaginal candidiasis cases. Although there is no case report in which anidulafungin is used for treatment and it should be kept in mind that the anidulafungin is also in the treatment as it is summarized. Disclosures All authors: No reported disclosures.

scholarly journals Reversible Deficiency of Antimicrobial Polypeptides in Bacterial Vaginosis

2006 ◽  
Vol 74 (10) ◽  
pp. 5693-5702 ◽  
Author(s):  
Erika V. Valore ◽  
Dorothy J. Wiley ◽  
Tomas Ganz
Keyword(s):  
Bacterial Vaginosis ◽  
Sexually Transmitted Diseases ◽  
Lavage Fluid ◽  
Vulvovaginal Candidiasis ◽  
Gardnerella Vaginalis ◽  
Vaginal Fluid ◽  
Sexually Transmitted ◽  
Lactobacillus Jensenii ◽  
Increased Risk ◽  
Antimicrobial Polypeptides

ABSTRACT Bacterial vaginosis is a common condition associated with increased risk of sexually transmitted diseases, including human immunodeficiency virus infections. In contrast, vulvovaginal candidiasis has a much weaker association with sexually transmitted diseases. We found that vaginal lavage fluid from women with bacterial vaginosis is deficient in antimicrobial polypeptides and antimicrobial activity compared to fluid from healthy women or women with vulvovaginal candidiasis. Effective treatment normalized the concentrations of antimicrobial polypeptides in both bacterial vaginosis and in vulvovaginal candidiasis, suggesting that the abnormalities were a result of the diseases. Unlike in vulvovaginal candidiasis, the neutrophil attractant chemokine interleukin-8 (IL-8) was not increased in bacterial vaginosis, accounting for low concentrations of neutrophil-derived defensins in vaginal fluid. In organotypic cultures of human vaginal epithelium containing dendritic cells, treatment with Lactobacillus jensenii, a typical vaginal resident, induced the synthesis of IL-8 mRNA and the epithelial human β-defensin-2 mRNA, but a typical bacterial vaginosis pathogen, Gardnerella vaginalis, had no effect. When the two bacteria were combined, Gardnerella vaginalis did not interfere with the immunostimulatory effect of Lactobacillus jensenii. The loss of normal immunostimulatory flora in bacterial vaginosis is thus associated with a local deficiency of multiple innate immune factors, and this deficiency could predispose individuals to sexually transmitted diseases.

Molecular Characterization of Highly Susceptible Candida africana from Vulvovaginal Candidiasis

2015 ◽  
Vol 180 (5-6) ◽  
pp. 317-323 ◽  
Author(s):  
Seyed Amir Yazdanparast ◽  
Sadegh Khodavaisy ◽  
Hamed Fakhim ◽  
Tahereh Shokohi ◽  
Iman Haghani ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Vulvovaginal Candidiasis ◽  
Candida Africana

scholarly journals Speciation and antifungal susceptibility testing of Candida isolates in various clinical samples in a tertiary care hospital in Mumbai

2018 ◽  
Vol 9 (3) ◽  
pp. 106 ◽  
Author(s):  
Sunayana Mukesh Jangla ◽  
Raji Naidu ◽  
Sofia C. Patel
Keyword(s):  
Candida Albicans ◽  
Amphotericin B ◽  
Candida Species ◽  
Antifungal Susceptibility ◽  
Tertiary Care ◽  
Vaginal Swab ◽  
Clinical Samples ◽  
Production Test ◽  
Care Hospital ◽  
Urease Test

Background: Over the last few years fungal infection rates have increased and a change is seen in their epidemiology and antifungal susceptibility pattern. Hence this study was conducted to learn the distribution of Candida species in various samples and their antifungal susceptibility pattern.Material and Methods: A total of 60Candida isolates were included in the study. Identification was done by colony morphology and Gram stain. Speciation was carried out by Germ-tube test, urease test, chlamydoconidia production test, colony characteristics on chromogenic agar medium, sugar assimilation test,sugar fermentation testand Vitek2 compact(Biomeriux, France) using ID-YST cards. Antifungal testing was done on Vitek2 compact using AST YS01 cards which included fluconazole, voriconazole, amphotericin b, caspofungin, micafungin and flucytosine.Results: 60 Candida isolates were included in this study. Samples from which Candida species were isolated were sputum (45%), urine (33.5%), pus (12%), vaginal swab (5%), endotracheal secretion (1.5%), blood (1.5%) and tissue (1.5%). Isolates from males and females were 30% and 70% respectively. Isolates from geriatric age group (>65 years) and adults (18-65 years) were 52% and 48% respectively. Isolates from samples received from IPD, OPD and ICU were 58%, 34% and 8% respectively. Out of all isolates, Candida albicans was 58%, Candida tropicalis 20%, Candida glabrata 10%, Candida parapsilosis 9% and Candida krusei3%. All Candida species (except Candidaglabrata) showed 100% sensitivity to amphotericin b and caspofungin. Sensitivity to azole group of drugs was 100% among NAC except C. glabrata and C. krusei and more than 90% among C. albicans.Conclusion: Candida albicans was the commonest isolate followed by C. tropicalis among the NAC. Overall also, C. Albicans were predominant as compared to Non albicans Candida (NAC) species. All Candidaisolates except (C. glabrata) showed good sensitivity to all antifungals.

Vaginal microbiological flora and sexually transmitted diseases in women with recurrent or current vulvovaginal candidiasis

Infection ◽  
1995 ◽  
Vol 23 (2) ◽  
pp. 81-84 ◽  
Author(s):  
Birgitta Zdolsek ◽  
D. Hellberg ◽  
S. Nilsson ◽  
G. Fröman ◽  
P. A. Mårdh
Keyword(s):  
Sexually Transmitted Diseases ◽  
Vulvovaginal Candidiasis ◽  
Sexually Transmitted ◽  
Microbiological Flora
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