scholarly journals Study of the Structure, Oxygen-Transporting Functions, and Ionic Composition of Erythrocytes at Vascular Diseases

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Viktor V. Revin ◽  
Natalia V. Gromova ◽  
Elvira S. Revina ◽  
Natalya A. Mel’nikova ◽  
Larisa A. Balykova ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Ionic Composition ◽  
Vascular Diseases ◽  
Interference Microscopy ◽  
Structure And Properties ◽  
Laser Interference ◽  
Raman Scattering Spectroscopy ◽  
Composition And Structure ◽  
Laser Interference Microscopy

The present paper explores the role of erythrocytes in the pathogenesis of vascular diseases. The state of erythrocytes, their ionic composition and structure, and properties of erythrocytes hemoglobin were studied by using laser interference microscopy, Raman scattering spectroscopy, and capillary electrophoresis. In patients suffering from vascular disorders we identified statistically significant changes in the shape of erythrocytes, their ionic composition, and redistribution of hemoglobin throughout cells.

scholarly journals Role of Membrane Lipids in the Regulation of Erythrocytic Oxygen-Transport Function in Cardiovascular Diseases

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Victor V. Revin ◽  
Natalia V. Gromova ◽  
Elvira S. Revina ◽  
Maria I. Martynova ◽  
Angelina I. Seikina ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Cardiovascular Diseases ◽  
Oxygen Transport ◽  
Membrane Lipids ◽  
Beta Blockers ◽  
Interference Microscopy ◽  
Transport Function ◽  
Laser Interference ◽  
Laser Interference Microscopy

The composition and condition of membrane lipids, the morphology of erythrocytes, and hemoglobin distribution were explored with the help of laser interference microscopy (LIM) and Raman spectroscopy. It is shown that patients with cardiovascular diseases (CVD) have significant changes in the composition of their phospholipids and the fatty acids of membrane lipids. Furthermore, the microviscosity of the membranes and morphology of the erythrocytes are altered causing disordered oxygen transport by hemoglobin. Basic therapy carried out with the use of antiaggregants, statins, antianginals, beta-blockers, and calcium antagonists does not help to recover the morphofunctional properties of erythrocytes. Based on the results the authors assume that, for the relief of the ischemic crisis and further therapeutic treatment, it is necessary to include, in addition to cardiovascular disease medicines, medication that increases the ability of erythrocytes’ hemoglobin to transport oxygen to the tissues. We assume that the use of LIM and Raman spectroscopy is advisable for early diagnosis of changes in the structure and functional state of erythrocytes when cardiovascular diseases develop.

Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study

2008 ◽  
Vol 13 (3) ◽  
pp. 034004 ◽  
Author(s):  
Alexey R. Brazhe ◽  
Nadezda A. Brazhe ◽  
Georgy V. Maksimov ◽  
Pavel S. Ignatyev ◽  
Andrey B. Rubin ◽  
...  
Keyword(s):  
Phase Modulation ◽  
Cell Imaging ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

Real time laser interference microscopy for bar-spread polystyrene/poly(methyl methacrylate) blends

2014 ◽  
Vol 52 (15) ◽  
pp. 985-992 ◽  
Author(s):  
Ehtsham Ul Haq ◽  
Daniel T. W. Toolan ◽  
Jillian A. Emerson ◽  
Thomas H. Epps ◽  
Jonathan R. Howse ◽  
...  
Keyword(s):  
Methyl Methacrylate ◽  
Real Time ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Poly Methyl Methacrylate ◽  
Laser Interference Microscopy

Diagnostics possibilities of erythrocytes analysis by the method of laser interference microscopy

2021 ◽  
Vol 66 (1) ◽  
pp. 22-25
Author(s):  
A. V. Deryugina ◽  
M. N. Ivashchenko ◽  
P. S. Ignatyev ◽  
A. A. Belov ◽  
V. A. Petrov
Keyword(s):  
Functional Activity ◽  
Interference Microscopy ◽  
Qualitative Assessment ◽  
Critical Conditions ◽  
Laser Interference ◽  
Functional Capabilities ◽  
Surface Nanostructure ◽  
Laser Interference Microscopy ◽  
Microscopy Method ◽  
Altered Cell

The morphofunctional state of erythrocytes significantly changes by the onset and progression of various diseases, as well as in critical conditions, injuries, accompanied by severe tissue damage. This leads to a violation of microcirculation and has an effect on hemorheology in general. In connection with this, there is a need for the development of adequate methods for assessing the functional activity of erythrocytes. The aim of the work was to study the possibilities of laser interference microscopy in the analysis of the functional state of erythrocytes. The results allowed us to quantify the state of the phase height and phase diameter of erythrocytes. Analysis of the surface nanostructure gave a qualitative assessment of the functional activity of cells. The following patterns were revealed: the action of adrenaline causes the appearance of echinocytes with an increase in phase height and decrease phase diameter and the appearance of spicules on the surface of cells. The action of cortisol leads to a decrease in the phase height and the phase diameter with the appearance of numerous loosening of the structure of the cell surface. Moreover, the traditional method of analyzing the distribution of erythrocytes in a population by morphology (discocytes, stomatocytes, and degeneratively altered cell forms) did not reveal statistically significant differences. Thus, the results indicate that the interference microscopy method allows a qualitative assessment of the morphofunctional activity of native cells. This increases the information content of the analysis and leads to the objectification of data on the functional capabilities of erythrocytes.

Use of laser interference microscopy to study the state of UV(B)-irradiated mouse peritoneal macrophages

2012 ◽  
Vol 55 (6) ◽  
pp. 726-730 ◽  
Author(s):  
M. V. Zagubizhenko ◽  
A. I. Yusipovich ◽  
S. K. Pirutin ◽  
V. L. Minaev ◽  
Yu. B. Kudryashov
Keyword(s):  
Peritoneal Macrophages ◽  
The State ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy ◽  
Mouse Peritoneal Macrophages

Assessing the Functional State of Red Blood Cells by Using the Laser Interference Microscopy

2021 ◽  
Vol 15 (4) ◽  
pp. 388-392
Author(s):  
A. V. Deryugina ◽  
M. N. Ivashchenko ◽  
A. A. Belov ◽  
P. S. Ignatiev ◽  
V. B. Metelin
Keyword(s):  
Red Blood Cells ◽  
Functional State ◽  
Blood Cells ◽  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

VITAL MORPHOMETY OF RBC IN THEIR INTERACTION WITH STRESS HORMONES

2020 ◽  
pp. 146-147
Author(s):  
A. Deryugina ◽  
P. Ignatiev ◽  
V. Metelin ◽  
A. Belov ◽  
V. Petrov
Keyword(s):  
Normal State ◽  
Physiological State ◽  
Stress Hormones ◽  
Interference Microscopy ◽  
Extreme Condition ◽  
Laser Interference ◽  
Laser Interference Microscopy ◽  
As Stress ◽  
To Receive

The possibility to use the laser interference microscopy in order to assess the RBCs morphology in normal state and in interaction with cortisol and adrenalin as stress hormones was studied. It was established that the laser interference microscopy represented an important instrument permitting to receive the information about physiological state of the RBCs as well as of the organism in the whole in extreme condition. New aspects of cortisol and adrenalin impact on the RBC morphology are defined

Laser interference microscopy in erythrocyte study

2009 ◽  
Vol 105 (10) ◽  
pp. 102037 ◽  
Author(s):  
A. I. Yusipovich ◽  
E. Yu. Parshina ◽  
N. Yu. Brysgalova ◽  
A. R. Brazhe ◽  
N. A. Brazhe ◽  
...  
Keyword(s):  
Interference Microscopy ◽  
Laser Interference ◽  
Laser Interference Microscopy

Study of cancer and normal cells dynamics based on laser interference microscopy

2020 ◽  
Author(s):  
Aleksander S. Nikitiuk ◽  
Anna O. Voronina ◽  
Yuliya A. Beloglazova ◽  
Olga S. Ozernykh ◽  
Victoria V. Grishko ◽  
...  
Keyword(s):  
Interference Microscopy ◽  
Laser Interference ◽  
Normal Cells ◽  
Laser Interference Microscopy
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