scholarly journals Dynamic Tracking Human Mesenchymal Stem Cells Tropism following Smoke Inhalation Injury in NOD/SCID Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
MeiJuan Song ◽  
Qi Lv ◽  
XiuWei Zhang ◽  
Juan Cao ◽  
ShuLi Sun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Inhalation Injury ◽  
Scid Mice ◽  
Alveolar Epithelial Cells ◽  
Smoke Inhalation ◽  
Control Group ◽  
Smoke Inhalation Injury

Multiple preclinical evidences have supported the potential value of mesenchymal stem cells (MSCs) for treatment of acute lung injury (ALI). However, few studies focus on the dynamic tropism of MSCs in animals with acute lung injury. In this study, we track systemically transplanted human bone marrow-derived mesenchymal stem cells (hBMSCs) in NOD/SCID mice with smoke inhalation injury (SII) through bioluminescence imaging (BLI). The results showed that hBMSCs systemically delivered into healthy NOD/SCID mouse initially reside in the lungs and then partially translocate to the abdomen after 24 h. Compared with the uninjured control group treated with hBMSCs, higher numbers of hBMSCs were found in the lungs of the SII NOD/SCID mice. In both the uninjured and SII mice, the BLI signals in the lungs steadily decreased over time and disappeared by 5 days after treatment. hBMSCs significantly attenuated lung injury, elevated the levels of KGF, decreased the levels of TNF-αin BALF, and inhibited inflammatory cell infiltration in the mice with SII. In conclusion, our findings demonstrated that more systemically infused hBMSCs localized to the lungs in mice with SII. hBMSC xenografts repaired smoke inhalation-induced lung injury in mice. This repair was maybe due to the effect of anti-inflammatory and secreting KGF of hMSCs but not associated with the differentiation of the hBMSCs into alveolar epithelial cells.

scholarly journals Vitamin E attenuates acute lung injury in sheep with burn and smoke inhalation injury

Redox Report ◽  
2006 ◽  
Vol 11 (2) ◽  
pp. 61-70 ◽  
Author(s):  
Naoki Morita ◽  
Katsumi Shimoda ◽  
Maret G. Traber ◽  
Martin Westphal ◽  
Perenlei Enkhbaatar ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Vitamin E ◽  
Lung Injury ◽  
Inhalation Injury ◽  
Smoke Inhalation ◽  
Smoke Inhalation Injury

Influence of Nebulized Unfractionated Heparin and N-Acetylcysteine in Acute Lung Injury After Smoke Inhalation Injury

2009 ◽  
Vol 30 (2) ◽  
pp. 249-256 ◽  
Author(s):  
Andrew C. Miller ◽  
Abel Rivero ◽  
Sophia Ziad ◽  
David J. Smith ◽  
Elamin M. Elamin
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Unfractionated Heparin ◽  
Inhalation Injury ◽  
Smoke Inhalation ◽  
Smoke Inhalation Injury

scholarly journals Beneficial pulmonary effects of a metalloporphyrinic peroxynitrite decomposition catalyst in burn and smoke inhalation injury

2011 ◽  
Vol 300 (2) ◽  
pp. L167-L175 ◽  
Author(s):  
Matthias Lange ◽  
Csaba Szabo ◽  
Perenlei Enkhbaatar ◽  
Rhykka Connelly ◽  
Eszter Horvath ◽  
...  
Keyword(s):  
Lung Injury ◽  
Total Body Surface Area ◽  
Weight Ratio ◽  
Dry Weight ◽  
Inhalation Injury ◽  
Smoke Inhalation ◽  
Control Group ◽  
Significant Drop ◽  
Cytotoxic Effects ◽  
Smoke Inhalation Injury

During acute lung injury, nitric oxide (NO) exerts cytotoxic effects by reacting with superoxide radicals, yielding the reactive nitrogen species peroxynitrite (ONOO−). ONOO− exerts cytotoxic effects, among others, by nitrating/nitrosating proteins and lipids, by activating the nuclear repair enzyme poly(ADP-ribose) polymerase and inducing VEGF. Here we tested the effect of the ONOO− decomposition catalyst INO-4885 on the development of lung injury in chronically instrumented sheep with combined burn and smoke inhalation injury. The animals were randomized to a sham-injured group ( n = 7), an injured control group [48 breaths of cotton smoke, 3rd-degree burn of 40% total body surface area ( n = 7)], or an injured group treated with INO-4885 ( n = 6). All sheep were mechanically ventilated and fluid-resuscitated according to the Parkland formula. The injury-related increases in the abundance of 3-nitrotyrosine, a marker of protein nitration by ONOO−, were prevented by INO-4885, providing evidence for the neutralization of ONOO− action by the compound. Burn and smoke injury induced a significant drop in arterial Po2-to-inspired O2 fraction ratio and significant increases in pulmonary shunt fraction, lung lymph flow, lung wet-to-dry weight ratio, and ventilatory pressures; all these changes were significantly attenuated by INO-4885 treatment. In addition, the increases in IL-8, VEGF, and poly(ADP-ribose) in lung tissue were significantly attenuated by the ONOO− decomposition catalyst. In conclusion, the current study suggests that ONOO− plays a crucial role in the pathogenesis of pulmonary microvascular hyperpermeability and pulmonary dysfunction following burn and smoke inhalation injury in sheep. Administration of an ONOO− decomposition catalyst may represent a potential treatment option for this injury.

Cutaneous burn diminishes beneficial effect of intravenously administered mesenchymal stem cells on acute lung injury induced by smoke inhalation in sheep

Burns ◽  
2020 ◽  
Vol 46 (8) ◽  
pp. 1914-1923
Author(s):  
Tuvshintugs Baljinnyam ◽  
Enkhtuya Radnaa ◽  
Yosuke Niimi ◽  
Satoshi Fukuda ◽  
Donald S. Prough ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Acute Lung Injury ◽  
Lung Injury ◽  
Beneficial Effect ◽  
Smoke Inhalation

Pathophysiology of acute lung injury in combined burn and smoke inhalation injury

2004 ◽  
Vol 107 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Perenlei ENKHBAATAR ◽  
Daniel L. TRABER
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Burn Injury ◽  
Surgical Excision ◽  
Inhalation Injury ◽  
Burn Injuries ◽  
Morbidity And Mortality ◽  
Smoke Inhalation ◽  
Therapeutic Approaches ◽  
Smoke Inhalation Injury

In the U.S.A., more than 1 million burn injuries occur every year. Although the survival from burn injury has increased in recent years with the development of effective fluid resuscitation management and early surgical excision of burned tissue, the mortality of burn injury is still high. In these fire victims, progressive pulmonary failure and cardiovascular dysfunction are important determinants of morbidity and mortality. The morbidity and mortality increases when burn injury is associated with smoke inhalation. In the present review, we will describe the pathophysiological aspects of acute lung injury induced by combined burn and smoke inhalation and examine various therapeutic approaches.

AEROSOLIZED ALPHA-TOCOPHEROL AMELIORATES ACUTE LUNG INJURY FOLLOWING COMBINED BURN AND SMOKE INHALATION INJURY IN SHEEP

Shock ◽  
2006 ◽  
Vol 25 (3) ◽  
pp. 277-282 ◽  
Author(s):  
Naoki Morita ◽  
Maret G. Traber ◽  
Perenlei Enkhbaatar ◽  
Martin Westphal ◽  
Kazunori Murakami ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Inhalation Injury ◽  
Alpha Tocopherol ◽  
Smoke Inhalation ◽  
Smoke Inhalation Injury
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