Oxidative Stress Implications in the Affective Disorders: Main Biomarkers, Animal Models Relevance, Genetic Perspectives, and Antioxidant Approaches

The correlation between the affective disorders and the almost ubiquitous pathological oxidative stress can be described in a multifactorial way, as an important mechanism of central nervous system impairment. Whether the obvious changes which occur in oxidative balance of the affective disorders are a part of the constitutive mechanism or a collateral effect yet remains as an interesting question. However it is now clear that oxidative stress is a component of these disorders, being characterized by different aspects in a disease-dependent manner. Still, there are a lot of controversies regarding the relevance of the oxidative stress status in most of the affective disorders and despite the fact that most of the studies are showing that the affective disorders development can be correlated to increased oxidative levels, there are various studies stating that oxidative stress is not linked with the mood changing tendencies. Thus, in this minireview we decided to describe the way in which oxidative stress is involved in the affective disorders development, by focusing on the main oxidative stress markers that could be used mechanistically and therapeutically in these deficiencies, the genetic perspectives, some antioxidant approaches, and the relevance of some animal models studies in this context.

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Oxidative stress markers in affective disorders

Pharmacological Reports ◽

10.1016/s1734-1140(13)71517-2 ◽

2013 ◽

Vol 65 (6) ◽

pp. 1558-1571 ◽

Cited By ~ 58

Author(s):

Marcin Siwek ◽

Magdalena Sowa-Kućma ◽

Dominika Dudek ◽

Krzysztof Styczeń ◽

Bernadeta Szewczyk ◽

...

Keyword(s):

Oxidative Stress ◽

Affective Disorders ◽

Oxidative Stress Markers ◽

Stress Markers

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The dynamics of some oxidative stress markers in 3, 6 and 12-months alcohol abstinent patients: possible relevance for the usage of antioxidants in alcohol withdrawal / Dinamica unor markeri ai stresului oxidativ la 3, 6 şi 12 luni de abstinenţă de la alcool: posibila relevanţă a utilizării de antioxidanţi

Revista Romana de Medicina de Laborator ◽

10.2478/rrlm-2014-0040 ◽

2014 ◽

Vol 22 (4) ◽

Cited By ~ 1

Author(s):

Florin Petrariu ◽

Ovidiu Alexinschi ◽

Roxana Chirita ◽

Vasile Chirita ◽

Alin Ciobica ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Alcohol Withdrawal ◽

Present Report ◽

Control Group ◽

Oxidative Stress Markers ◽

Significant Group ◽

Stress Markers ◽

Complex Processes ◽

Oxidative Stress Status

AbstractWhile the exact relevance of the oxidative stress markers after the complex processes of alcohol withdrawal is still controversial, in the present report we were interested in studying the relevance of oxidative stress status in the alcohol withdrawal processes, by determining some oxidative stress markers after 3, 6 and 12 months of abstinence. 62 patients were selected, all of them males. Thus, 33 (baseline), 14 (3 months), 14 (6 months) and 15 (12 months) patients, while the control group (n=32) included healthy, sex and aged-matched subjects. Regarding superoxid dismutase, we observed a significant group difference (p<0.0001), together with an increase in all 3 cases of time-abstinence, as compared to baseline results: (p<0.0001-3 months), (p<0.0001-6 months) and (p<0.0001- 12 months). Also for glutathione peroxidase, we observed a significant overall effect of the abstinence in our groups (p=0.0003), plus an increase especially at 6 months (p=0.03) and 12 months (p=0.006). Regarding malondialdehyde, as a main marker for the lipid peroxidation processes, we found significant differences between our groups (p<0.0001), together with a decrease in all 3 cases, compared to the baseline group (p=0.003), (p=0.01) and (p=0.0002). In conclusion, this confirms the increased oxidative stress status in alcoholic patients and even more importantly, we showed that there is a significant and progressive decrease in the oxidative stress status at 3, 6 and 12 months after the withdrawal process, as demonstrated by the increased levels of antioxidant enzymes and decreased rate of lipid peroxidation, when compared to baseline values.

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Mixed Exposure of Persistent Organic Pollutants Alters Oxidative Stress Markers and Mitochondrial Function in the Tail of Zebrafish Depending on Sex

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18189539 ◽

2021 ◽

Vol 18 (18) ◽

pp. 9539

Author(s):

Songhee Lee ◽

Eun Ko ◽

Hyojin Lee ◽

Ki-Tae Kim ◽

Moonsung Choi ◽

...

Keyword(s):

Oxidative Stress ◽

Metabolic Disease ◽

Organic Pollutants ◽

Persistent Organic Pollutants ◽

Oxidative Stress Marker ◽

Functional Markers ◽

Dependent Manner ◽

Oxidative Stress Markers ◽

Aroclor 1254 ◽

Stress Markers

Persistent organic pollutants (POPs) are lipid-soluble toxins that are not easily degraded; therefore, they accumulate in the environment and the human body. Several studies have indicated a correlation between POPs and metabolic diseases; however, their effects on mitochondria as a central organelle in cellular metabolism and the usage of mitochondria as functional markers for metabolic disease are barely understood. In this study, a zebrafish model system was exposed to two subclasses of POPs, organochlorine pesticides (OCPs) and polychlorinated biphenyls (PCBs), under two different conditions (solitary OCPs or OCPs with PCBs (Aroclor 1254)), and changes in the oxidative stress marker levels and mitochondrial enzyme activities in the electron transport chain of the tail were measured to observe the correlation between POPs and representative biomarkers for metabolic disease. The results indicated different responses upon exposure to OCPs and OCPs with Aroclor 1254, and accelerated toxicity was observed following exposure to mixed POPs (OCPs with Aroclor 1254). Males were more sensitive to changes in the levels of oxidative stress markers induced by POP exposure, whereas females were more susceptible to the toxic effects of POPs on the levels of mitochondrial activity markers. These results demonstrate that the study reflects real environmental conditions, with low-dose and multiple-toxin exposure for a long period, and that POPs alter major mitochondrial enzymes’ functions with an imbalance of redox homeostasis in a sex-dependent manner.

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Preliminary Data Regarding the Effects of Oxytocin Administration on the Oxidative Stress Status of Zebrafish (Danio Rerio)

Revista de Chimie ◽

10.37358/rc.17.7.5734 ◽

2017 ◽

Vol 68 (7) ◽

pp. 1640-1643 ◽

Cited By ~ 1

Author(s):

Ioana Miruna Balmus ◽

Stefan Strungaru ◽

Mircea Nicoara ◽

Gabriel Plavan ◽

Sabina Ioana Cojocaru ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Danio Rerio ◽

Oxidative Stress Markers ◽

Adult Zebrafish ◽

Stress Markers ◽

Oxidative Stress Status ◽

Influence Of Water ◽

Biology Research ◽

Different Doses

Zebrafish are important animal models widely used in molecular biology research. To our best knowledge, no previous study on OT effects in zebrafishes� oxidative stress status was performed. Moreover, while the zebrafish are naturally producing isotocin, the investigation of its homologue OT effects may provide consistent evidence of the possible influence of oxytocin in isotocin naturally producing teleosteens. In this way, we aimed to analyse the influence of water exposure (e.g. branchial and tegmental exposure) on two different doses of OT (33.2 ng/mL and 66.4 ng/mL) on the adult zebrafish (n = 15) oxidative stress markers. Significant differences and correlations were found in the zebrafish exposed to a higher OT concentration, although lipid peroxidation tended to decrease as the OT concentration increased. Thus, it seems that oxytocin is cross-reacting and exerts clear effect on oxidative stress status main parameters in zebrafish.

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The effects of a 6-OHDA induced lesion in murine nuccleus accumbens on memory and oxidative stress status

Open Medicine ◽

10.2478/s11536-013-0165-6 ◽

2013 ◽

Vol 8 (4) ◽

pp. 443-449

Author(s):

Radu Lefter ◽

Alin Ciobica ◽

Lucian Hritcu ◽

Bogdan Stoica ◽

Dumitru Cojocaru ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Antioxidant Status ◽

Total Antioxidant Status ◽

Oxidative Stress Markers ◽

Maze Task ◽

Stress Markers ◽

Oxidative Stress Status ◽

Total Antioxidant ◽

Dopaminergic Structure

AbstractNucleus accumbens (NAcc) are a collection of neurons that form the main part of the ventral striatum, which is a significant dopaminergic structure. Also, NAcc is thought to play an important role in reward, pleasure, laughter, addiction, aggression, fear, and the placebo effect. In the present work we were interested in studying the effects of a 6-OHDA induced lesion in the nucleus accumbens (NAcc), which is known as an important dopaminergic structure, on a specific behavioral task that involves both short term and long term spatial memory (the radial-8-arm-maze task), as well as on the oxidative stress markers (two antioxidant enzymes: superoxide dismutase-SOD and glutathione peroxidase-GPX and a lipid peroxidation marker: malondialdehyde-MDA, as well as the total antioxidant status-TAS) from the temporal lobe, which is considered to be the most vulnerable cortical area to oxygen levels fluctuations and hypoxia. Our results showed some significant effects of this lesion on the reference memory errors and time necessary to finish the test in the radial-8-arm-maze task. Additionally, increased oxidative stress status was demonstrated in the temporal lobe of the lesioned rats, as demonstrated by the high levels of lipid peroxidation and decreased total antioxidant status. Moreover, significant correlations are reported here between the behavioral parameters which we studied in the radial-8-arm-maze task and the aforementioned oxidative stress markers.

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Comparison of the deleterious effects of yaji and cadmium chloride on testicular physiomorphological and oxidative stress status: The gonadoprotective effects of an omega-3 fatty acid

Clinical and Experimental Reproductive Medicine ◽

10.5653/cerm.2019.03517 ◽

2020 ◽

Vol 47 (3) ◽

pp. 168-179

Author(s):

Ehitare Ikekhuamen Ekhoye ◽

Samson Eshikhokhale Olerimi ◽

Santos Ehizokhale Ehebha

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Semen Quality ◽

Gonadosomatic Index ◽

Follicle Stimulating Hormone ◽

Oxidative Stress Markers ◽

Omega 3 ◽

Stress Markers ◽

Oxidative Stress Status ◽

And Oxidative Stress

Objective: This study investigated testicular oxidative stress status and physiomorphological function in Wistar rats fed with yaji and cadmium chloride (CdCl2).Methods: Sixty male albino Wistar rats (12 per group) were randomly assigned to five groups: group I (control), group II (300 mg/kg.bw of yaji), group III (500 mg/kg.bw of yaji), group IV (2.5 mg/kg.bw of CdCl2), and group V (2.5 mg/kg.bw of yaji+4 mg/kg.bw omega-3). Each group was evenly subdivided into two subgroups and treatment was administered for 14 days and 42 days, respectively. Semen quality (sperm count, progressive motility, normal morphology, and gonadosomatic index), hormones (testosterone, follicle-stimulating hormone, and luteinizing hormone), testicular oxidative stress markers (superoxide dismutase, catalase, glutathione peroxidase, and malonaldehyde) and testicular histomorphological features were examined.Results: Yaji caused significant (p< 0.05) dose- and duration-dependent reductions in semen quality, the gonadosomatic index, testosterone, follicle-stimulating hormone, and luteinizing hormone. Yaji also caused significant (p< 0.05) dose- and duration-dependent decreases in superoxide dismutase, catalase, and glutathione peroxidase activity, as well as increased testicular malonaldehyde levels. Yaji induced distortions in the testicular histological architecture. CdCl2 damaged testicular function by significantly (p< 0.05) reducing semen quality, reproductive hormone levels, and oxidative stress markers in albino Wistar rats. CdCl2 also altered the histology of the testis.Conclusion: This study shows that yaji sauce has similar anti-fertility effects to those of CdCl2, as it adversely interferes with male reproduction by impairing oxidative stress markers and the function and morphological features of the testis.

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Increased oxidative stress status in rat serum after five minutes treadmill exercise

Open Medicine ◽

10.2478/s11536-013-0329-4 ◽

2014 ◽

Vol 9 (5) ◽

pp. 722-728 ◽

Cited By ~ 2

Author(s):

Florin-Petrut Trofin ◽

Alin Ciobica ◽

Dumitru Cojocaru ◽

Marin Chirazi ◽

Cezar Honceriu ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Antioxidant Status ◽

Specific Activity ◽

Total Antioxidant Status ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Oxidative Stress Status ◽

Total Antioxidant

AbstractAlthough it is accepted that an important correlation exists between the physical exercise and the oxidative stress status, the data regarding the levels of the main oxidative stress markers after physical training have been difficult to interpret and a subject of many controversies. There are also very few studies regarding the effects of short-time exercise on the oxidative stress status modifications. Thus, in the present report we were interested in studying the modifications of some oxidative stress markers (two antioxidant enzymes-superoxide dismutase and glutathione peroxidase, a lipid peroxidation parameter — malondyaldehide, the total antioxidant status and protein carbonyl levels), from the serum of rats that were subject to one bout of five minutes exercise on a treadmill, when compared to a control sedentary group. In this way, we observed a decrease of superoxide dismutase specific activity in the rats which performed the exercises. Still, no modifications of glutathione peroxidase specific activity were found between groups. In addition, increased levels of malondyaldehide and protein carbonyls were observed in the rats subjected to exercises. In conclusion, our data provides new evidence regarding the increase of the oxidative stress status, as a result of a 5-minutes bout of treadmill exercising in rats, expressed through a decrease in the SOD specific activity and the total antioxidant status and also an increase of the lipid peroxidation and protein oxidation processes.

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Detecting Oxidative Stress Biomarkers in Neurodegenerative Disease Models and Patients

Methods and Protocols ◽

10.3390/mps3040066 ◽

2020 ◽

Vol 3 (4) ◽

pp. 66

Author(s):

Yulia Sidorova ◽

Andrii Domanskyi

Keyword(s):

Oxidative Stress ◽

Animal Models ◽

Defense Mechanisms ◽

Oxidative Stress Markers ◽

Oxidative Stress Biomarkers ◽

Simple Method ◽

Neuronal Populations ◽

Stress Markers ◽

Preclinical Animal Models ◽

Detection And Localization

Oxidative stress is prominent in many neurodegenerative diseases. Along with mitochondrial dysfunction and pathological protein aggregation, increased levels of reactive oxygen and nitrogen species, together with impaired antioxidant defense mechanisms, are frequently observed in Alzheimer’s, Parkinson’s, Huntington’s disease and amyotrophic lateral sclerosis. The presence of oxidative stress markers in patients’ plasma and cerebrospinal fluid may aid early disease diagnoses, as well as provide clues regarding the efficacy of experimental disease-modifying therapies in clinical trials. In preclinical animal models, the detection and localization of oxidatively damaged lipids, proteins and nucleic acids helps to identify most vulnerable neuronal populations and brain areas, and elucidate the molecular pathways and the timeline of pathology progression. Here, we describe the protocol for the detection of oxidative stress markers using immunohistochemistry on formaldehyde-fixed, paraffin-embedded tissue sections, applicable to the analysis of postmortem samples and tissues from animal models. In addition, we provide a simple method for the detection of malondialdehyde in tissue lysates and body fluids, which is useful for screening and the identification of tissues and structures in the nervous system which are most affected by oxidative stress.

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