scholarly journals Profiling of the Predicted Circular RNAs in Ductal In Situ and Invasive Breast Cancer: A Pilot Study

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Marco Galasso ◽  
Giorgio Costantino ◽  
Lorenzo Pasquali ◽  
Linda Minotti ◽  
Federica Baldassari ◽  
...  
Keyword(s):  
Ductal Carcinoma ◽  
Circular Rnas ◽  
Next Generation ◽  
Genomic Context ◽  
Functional Roles ◽  
In Situ Carcinoma ◽  
The Common ◽  
Generation Sequencing ◽  
Invasive Breast Ductal Carcinoma

The recent advantage obtained by next generation sequencing allows a depth investigation of a new “old” kind of noncoding transcript, the circular RNAs. Circular RNAs are nontranslated RNAs, typically nonpolyadenylated, with a resistance to exonucleases that gives them the ability to be more stable than the common linear RNA isoforms. We used a bioinformatic detection tool (CIRCexplorer) to research predictive circRNAs from the next generation sequenced data of five samples of ductal in situ carcinoma (DCIS) and matched adjacent invasive ductal carcinoma (IDC). Furthermore, we also investigated the circular RNAs expressed in MCF7, an invasive breast ductal carcinoma cell line. We described the genomic context of the predicted circular RNAs and we address the hypothetical possible functional roles. This study showed a perspective of a panel of predictive circRNAs identified and the function that circRNAs could exert.

Acinic Cell Carcinoma of the Breast: Report of a Case With Immunohistochemical and Next-Generation Sequencing Studies

2021 ◽  
pp. 106689692110085
Author(s):  
Kaitlin D. Weaver ◽  
James Isom ◽  
Ashwini Esnakula ◽  
Karen Daily ◽  
Jaya R. Asirvatham
Keyword(s):  
Next Generation Sequencing ◽  
Cell Carcinoma ◽  
Triple Negative ◽  
Ductal Carcinoma ◽  
Left Breast ◽  
Acinic Cell Carcinoma ◽  
Next Generation ◽  
Carcinoma Of The Breast ◽  
Acinic Cell ◽  
Generation Sequencing

Acinic cell carcinoma of the breast is a rare subtype of triple-negative breast cancer that recapitulates the appearance of tumors seen in salivary glands. We present the case of a 42-year-old woman with an irregular, nontender mass above the left nipple during routine obstetric appointment at 24 weeks gestation. She was subsequently diagnosed with triple-negative invasive ductal carcinoma of the left breast, Nottingham grade 3, via core needle biopsy. She was treated with neoadjuvant therapy (doxorubucin and cyclophosphamide) antenatally and paclitaxel in the postpartum period followed by left mastectomy with sentinel node biopsy. The carcinoma in the mastectomy specimen showed a spectrum of morphologic patterns with immunohistochemistry revealing strong positivity for alpha-1-antichymotrypsin, epithelial membrane antigen (EMA), lysozyme, and S100. The histomorphology paired with the immunoprofile led us to the diagnosis of acinic cell carcinoma. We retrospectively performed immunostains in the core biopsy specimen, which demonstrated GATA-3 and DOG-1 positivity. Next-generation sequencing of the postneoadjuvant specimen using a 70-gene panel revealed 2 single-nucleotide variant (SNV) mutations: tumor protein 53 (TP53) (c.747G>T) SNV mutation and rearranged during transfection (RET) (c.2899G>A) SNV mutation.

Role of Ag NORs in Improve Diagnosis of Breast Cancer with Different grades and Subtypes’ among Sudanese women

2021 ◽  
Vol 1 (4) ◽  
pp. 443-448
Author(s):  
Doaa Ibrahim Ahmed
Keyword(s):  
Breast Cancer ◽  
Invasive Carcinoma ◽  
Ductal Carcinoma ◽  
Lobular Carcinoma ◽  
Age Group ◽  
Lobular Carcinoma In Situ ◽  
Tissue Sections ◽  
In Situ Carcinoma

This study aimed to evaluate the role of Ag NORs in improves diagnosis of Breast cancer with different subtypes’ among Sudanese Patients. This study include tissue sections of breast cancer diagnosed women, they were 30, ductal and lobular invasive carcinoma were 10 for each, while ductal and lobular in-situ carcinoma were 5 each. Found correlation between subtypes of breast cancer and Ag NOR , Invasive ductal carcinoma had more NOR while the lobular carcinoma in situ was less one , Stage III most frequency than the other stage. Silver staining were performed and Ag-NOR were detected in ductal and lobular invasive carcinoma more than ductal and lobular in-situ carcinoma, grade III has more frequency of Ag-NOR than other stages, and no correlation found between Ag-NOR and age group

Novel and established EWSR1 gene fusions and associations identified by next-generation sequencing and fluorescence in-situ hybridization

2019 ◽  
Vol 93 ◽  
pp. 65-73 ◽  
Author(s):  
Melissa Krystel-Whittemore ◽  
Martin S. Taylor ◽  
Miguel Rivera ◽  
Jochen K. Lennerz ◽  
Long P. Le ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Next Generation Sequencing ◽  
Fluorescence In Situ Hybridization ◽  
Gene Fusions ◽  
Next Generation ◽  
Ewsr1 Gene ◽  
Generation Sequencing

Radiation-Associated Primary Osteosarcoma of the Breast

Pathobiology ◽  
2020 ◽  
Vol 87 (5) ◽  
pp. 322-326
Author(s):  
Helen Bartlett ◽  
Maiar Elghobashy ◽  
Nayneeta Deshmukh ◽  
Rashmi Rao ◽  
Abeer M. Shaaban
Keyword(s):  
Ductal Carcinoma ◽  
Surgical Excision ◽  
Metaplastic Carcinoma ◽  
Post Irradiation ◽  
In Situ Carcinoma ◽  
Complete Surgical Excision ◽  
Spindle Cell Lesion ◽  
Phyllodes Tumour

<b><i>Introduction:</i></b> Non-epithelial primary mammary osteosarcomas are extremely rare. The differentials include metaplastic carcinoma and malignant phyllodes tumour. This is the first published case of primary breast osteosarcoma arising after local radiotherapy. <b><i>Case Presentation:</i></b> A 73-year-old female presented with a right-sided breast lump. The same breast had been irradiated 11 years previously for invasive ductal carcinoma. Diagnostic excision revealed a highly cellular, malignant spindle-cell lesion merged with an osteoid matrix and foci of calcification and bone formation. Immunohistochemistry and molecular studies showed no lines of differentiation. Due to the lack of epithelial/glandular differentiation, in situ carcinoma or leaf-like pattern, the diagnosis of post-irradiation osteosarcoma was made. She underwent mastectomy and is disease-free at 8 months of follow-up. <b><i>Conclusion:</i></b> Post-irradiation osteosarcoma should be considered in the differential diagnosis of breast lesions showing malignant osteoid. Extensive sampling and careful search for epithelial differentiation is required to guide management. Complete surgical excision is recommended.

scholarly journals Next generation sequencing of the clonal IGH rearrangement detects ongoing mutations and interfollicular trafficking in in situ follicular neoplasia

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0178503 ◽  
Author(s):  
Perikles Kosmidis ◽  
Irina Bonzheim ◽  
Claudia Dufke ◽  
Sema Colak ◽  
Thomas Hentrich ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Igh Rearrangement ◽  
Generation Sequencing

scholarly journals Malignant Phyllodes Tumour with Liposarcomatous Differentiation, Invasive Tubular Carcinoma, and Ductal and Lobular Carcinoma In Situ: Case Report and Review of the Literature

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Mardiana Abdul Aziz ◽  
Frank Sullivan ◽  
Michael J. Kerin ◽  
Grace Callagy
Keyword(s):  
Carcinoma In Situ ◽  
Ductal Carcinoma ◽  
Lobular Carcinoma ◽  
Lobular Carcinoma In Situ ◽  
Tubular Carcinoma ◽  
In Situ Carcinoma ◽  
Phyllodes Tumour ◽  
Radiotherapy Treatment ◽  
Malignant Phyllodes Tumour

A 43-year-old woman presented with a right breast lump that had enlarged over 5 months. She had chemoradiotherapy for non-Hodgkin’s lymphoma in 1989. Histology revealed a malignant phyllodes tumour (PT) with liposarcomatous differentiation and ductal carcinoma in situ (DCIS) within the tumour with invasive tubular carcinoma, DCIS, and lobular carcinoma in situ in the surrounding breast. She had surgery and adjuvant radiotherapy. One year follow-up showed no recurrence or metastatic disease. Liposarcomatous differentiation is uncommon in PTs, and coexisting carcinoma is rare with 38 cases in 31 reports in the literature. Carcinoma is reported in malignant (), benign () and in borderline PTs () with invasive carcinoma () and pure in situ carcinoma () recorded in equal frequency. Carcinoma is more commonly found within the confines of benign PTs; whereas it is more often found surrounding the PT or in the contralateral breast in malignant PTs. Previous radiotherapy treatment is reported in only two cases. The aetiology of co-existing carcinoma is unclear but the rarity of previous radiotherapy treatment suggests that it is incidental. This case highlights the diverse pathology that can occur with PTs, which should be considered when evaluating pathology specimens as they may impact on patient management.

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