scholarly journals HumanPapilloma VirusInfection in Patients with Male Accessory Gland Infection: Usefulness of the Ultrasound Evaluation

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Rosita A. Condorelli ◽  
Enzo Vicari ◽  
Laura M. Mongioi ◽  
Giorgio I. Russo ◽  
Giuseppe Morgia ◽  
...  
Keyword(s):  
Seminal Fluid ◽  
Hpv Infection ◽  
Accessory Gland ◽  
Seminal Vesicles ◽  
The Other ◽  
Male Accessory Gland ◽  
Papilloma Virus ◽  
Progressive Motility ◽  
Ultrasound Evaluation ◽  
Fluid Concentration

This study evaluated the ultrasound (US) features of 20 patients with MAGI and concomitantpapilloma virus(HPV) infection compared to 20 patients with microbial (presence ofChlamydia trachomatisalone) MAGI and 20 patients with amicrobial (inflammatory) MAGI. Patients with HPV infection showed significantly (p<0.05) higher total prostate, seminal vesicles, and epididymal US signs (18.0 ± 2.0) compared to the other 2 groups (12.0 ± 4.0 versus 10.0 ± 3.0, resp.). In addition, patients with MAGI and HPV had a higher prevalence of complicated forms of MAGI [prostatovesiculitis (PV) and prostate-vesiculo-epididymitis (PVE)] and a higher frequency of the fibrosclerotic variant compared to the other groups (70.0 ± 10.0% versus 48.0 ± 5.0% versus 15.0 ± 10.0%). Moreover, HPV infected patients had a higher number of US criteria suggestive for MAGI in the periurethral region of the prostate compared to the other groups. In particular, the patients showed a higher ratio between periurethral and lobar US criteria distribution (5.0 versus 0.5). Finally, the seminal fluid concentration ofCD45posleukocytes (2.0 ± 0.2 versus 1.3 ± 0.3 versus 1.0 ± 0.3 mil/mL) was significantly higher and sperm progressive motility was significantly lower in these patients compared to other groups.

Ejaculation and spermatophore formation in Culicoides melleus (Coq.) (Diptera: Ceratopogonidae)

1981 ◽  
Vol 59 (3) ◽  
pp. 332-346 ◽  
Author(s):  
J. R. Linley
Keyword(s):  
Electron Micrographs ◽  
Male Genitalia ◽  
Accessory Gland ◽  
Seminal Vesicles ◽  
Sperm Transfer ◽  
Male Accessory Gland ◽  
Male And Female ◽  
Membrane Complex

This paper describes the initial stages of sperm transfer in Culicoides melleus. A description of the male and female reproductive tracts is given and an account of events during the first 2 min of coitus. In this period the male secretes a spermatophore, which, during copulation, is held predominantly in the male genitalia. The course of ejaculation, involving passage of spermatozoa through the seminal vesicles and glutinous glands of the male accessory gland, and subsequently through the ejaculatory ducts, is described. Extrusion of the spermatophore at the aedeagus is illustrated and an explanation advanced to account for encapsulation of the sperm masses. The structure and content of the spermatophore is described with the aid of electron micrographs. A feature of particular interest is the "membrane complex," secreted prior to ejaculation by cells of the glutinous glands. The complex consists of a layer of membranes, which separates the ejaculate from the spermatophore walls.

scholarly journals Ultrasound evaluation of patients with male accessory gland inflammation: a pictorial review

Andrology ◽  
2021 ◽  
Author(s):  
Sandro La Vignera ◽  
Andrea Crafa ◽  
Rosita A. Condorelli ◽  
Federica Barbagallo ◽  
Laura M. Mongioì ◽  
...  
Keyword(s):  
Accessory Gland ◽  
Male Accessory Gland ◽  
Ultrasound Evaluation ◽  
Pictorial Review

scholarly journals Seminal Plasma Proteomic Biomarkers of Oxidative Stress

2020 ◽  
Vol 21 (23) ◽  
pp. 9113
Author(s):  
Rossella Cannarella ◽  
Andrea Crafa ◽  
Federica Barbagallo ◽  
Laura M. Mongioì ◽  
Rosita A. Condorelli ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Male Infertility ◽  
Seminal Plasma ◽  
Molecular Mechanisms ◽  
Seminal Fluid ◽  
Accessory Gland ◽  
Male Accessory Gland ◽  
Abnormal Sperm ◽  
Clinical Conditions ◽  
Biomarkers Of Oxidative Stress

The prevalence of idiopathic male infertility is high, up to 75% of patients with abnormal sperm parameters. Hence, the research of its causes is mandatory. Oxidative stress (OS) can be responsible for male infertility in 30–80% of cases. In recent years, seminal plasma (SP) proteomics has developed as a useful tool to provide biomarkers of specific diseases. This systematic review aims to collect the available evidence on the changes of SP proteome in patients exposed to OS to provide possible SP biomarkers of sperm OS. To accomplish this, the following keyterms “seminal fluid proteome”, “seminal plasma proteome”, “oxidative stress”, and “sperm oxidative stress” were used and 137 records were found. Among these, 17 were finally included. Nine proteins involved with OS were found overexpressed in patients with OS. Twenty-three proteins were found differentially expressed in patients with clinical conditions associated with OS, such as varicocele, male accessory gland infection/inflammation, cigarette smoke, and obesity. These proteins do not seem to overlap among the clinical conditions taken into account. We speculate that specific SP proteins may mediate OS in different clinical conditions. Altogether, these results suggest that proteomics could help to better understand some of the molecular mechanisms involved in the pathogenesis of infertility. However, further studies are needed to identify potential biomarkers of male infertility with valuable clinical significance.

Male accessory gland inflammation (MAGI): an evolving entity

2021 ◽  
pp. 205141582098768
Author(s):  
Hosam A. Noweir ◽  
Vaibhav Modgil ◽  
Ian Pearce
Keyword(s):  
Prostate Gland ◽  
Accessory Gland ◽  
Seminal Vesicles ◽  
Special Focus ◽  
Male Accessory Gland ◽  
Ductus Deferens ◽  
Inflammatory Conditions ◽  
Research Findings ◽  
Chronic Course

Male accessory gland infection (MAGI) is a generic acronym indicating inflammatory conditions affecting the prostate gland, seminal vesicles, the ductus deferens and the epididymis. It is a frequent disease, mostly with a chronic course. Majority of the MAGIs remain asymptomatic, thereby leading to a debate whether to treat these patients or not. The primary criterion for MAGI was suggested by Comhaire and colleagues in 1980. The role of MAGI in causing infertility and sexual dysfunctions has long been a matter of debate. The most recent studies show that MAGI could alter, with various mechanisms, both conventional and biofunctional sperm parameters, and determine worst reproductive outcome. This article provides an overview of up-to-date research findings about MAGI with special focus on data published on its impact on fertility; and diagnostic criteria including cellular and seminal biomarkers along with the promising results of emerging proteomic platforms for the identification of MAGI.

scholarly journals Male Accessory Gland Infection: Relevance of Serum Total Testosterone Levels

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
R. A. Condorelli ◽  
A. E. Calogero ◽  
E. Vicari ◽  
V. Favilla ◽  
S. Cimino ◽  
...  
Keyword(s):  
Multivariate Logistic Regression Analysis ◽  
Accessory Gland ◽  
The Other ◽  
Total Testosterone ◽  
Male Hypogonadism ◽  
Serum Concentrations ◽  
Male Accessory Gland ◽  
Independent Predictive Factor ◽  
Duration Of Symptoms

Aim of the present study was to evaluate the different ultrasound characterization of fertile symptomatic patients with MAGI (male accessory gland infection) according to different serum concentrations of total T (TT). We analyzed the ultrasound and hormonal data of 200 patients aged between 24.0 and 67.0 years. Patients were divided into six groups according to the sextile distribution of TT. Patients with serum concentrations of TT < 3.6 ng mL−1had a higher mean duration of symptoms compared to the other examined groups. Patients with serum concentrations of TT > 6.6 ng mL−1showed a frequency of ultrasound criteria suggestive for bilateral form of prostatitis and prostate-vesiculo-epididymitis and significantly lower compared to the other examined groups. At multivariate logistic regression analysis adjusted for age and BMI, TT was an independent predictive factor of prostatovesiculitis (OR = 0.818 [95% CI: 0.675–0.992];P<0.01) and prostate-vesiculo-epididymitis (OR = 0.714 [95% CI: 0.578–0.880];P<0.01), which represent the main forms of complicated MAGI. The results of this study suggest that male hypogonadism could be associated with a different ultrasound characterization of these patients.

scholarly journals Chronic Administration of Tadalafil Improves the Symptoms of Patients with Amicrobic MAGI: An Open Study

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Sandro La Vignera ◽  
Rosita A. Condorelli ◽  
Laura M. Mongioi ◽  
Aldo E. Calogero
Keyword(s):  
Pharmacological Treatment ◽  
Semen Analysis ◽  
Chronic Administration ◽  
Structured Interview ◽  
Accessory Gland ◽  
Male Accessory Gland ◽  
Delayed Ejaculation ◽  
Ultrasound Evaluation ◽  
Number Of Patients ◽  
Before And After

Aim of this study was to evaluate the effects of pharmacological treatment with Tadalafil 5 mg daily on symptoms and quality of sperm parameters in selected patients with amicrobic MAGI (male accessory gland inflammation). 120 patients with amicrobic MAGI (mean age 27.0 ± 6.0 years) with mild-moderate ED (erectile dysfunction) according to IIEF-5 (International Index of Erectile Function 5 Items) scores underwent pharmacological treatment with Tadalafil 5 mg daily for six months. Before and after treatment these patients were evaluated through IIEF-5, semen analysis (according to WHO Criteria, 2010), SI-MAGI (Structured Interview about Male Accessory Gland Inflammation), and ultrasound evaluation. Patients with PVE (prostate-vesciculo-epididymitis) showed a significant increase in the percentage of spermatozoa with total (16.0 ± 8.0 versus 30.0 ± 6.0%) and progressive motility (8.00 ± 10.0 versus 25.0 ± 6.00%). It was a significant reduction of the number of patients with complicated ultrasound forms (30.0 versus 52.0) and a significant increase of the number of patients with uncomplicated ultrasound form (90.0 versus 68.0). Finally, there was a significant reduction in the percentage of patients with alterations of sexual function different from DE, such as premature ejaculation (4.00 versus 8.00%), painful ejaculation (4.00 versus 10.0%), delayed ejaculation (12.50 versus 8.00%), and decreased libido (10.0 versus 25.0%).

scholarly journals Prevalence of human papilloma virus infection in patients with male accessory gland infection

2015 ◽  
Vol 30 (4) ◽  
pp. 385-391 ◽  
Author(s):  
S. La Vignera ◽  
E. Vicari ◽  
R.A. Condorelli ◽  
C. Franchina ◽  
G. Scalia ◽  
...  
Keyword(s):  
Virus Infection ◽  
Human Papilloma Virus ◽  
Human Papilloma Virus Infection ◽  
Accessory Gland ◽  
Male Accessory Gland ◽  
Papilloma Virus

scholarly journals FACS-based isolation and RNA extraction of Secondary Cells from the Drosophila male Accessory Gland

2019 ◽  
Author(s):  
Clément Immarigeon ◽  
François Karch ◽  
Robert K. Maeda
Keyword(s):  
Cell Population ◽  
Seminal Fluid ◽  
Rna Extraction ◽  
Cell Types ◽  
Accessory Gland ◽  
Specific Cell ◽  
Male Accessory Gland ◽  
Accessory Gland Proteins ◽  
Accessory Glands ◽  
Study Gene Expression

ABSTRACTTo appreciate the function of an organ, it is often critical to understand the role of rare cell populations. Unfortunately, this rarity often makes it difficult to obtain material for study. This is the case for the Drosophila male accessory gland, the functional homolog of mammalian prostate and seminal vesicle. In Drosophila, this gland is made up of two morphologically distinct cell types: the polygonally-shaped main cells, which compose 96% of the organ, and the larger, vacuole-containing secondary cells (SCs), which represent the remaining 4% of cells (~40 cells per lobe). Both cell types are known to produce accessory gland proteins (Acps), which are important components of the seminal fluid and are responsible for triggering multiple physiological and behavioral processes in females, collectively called the post-mating response (PMR). While a few genes are known to be specific to the SCs, the relative rarity of SCs has hindered the study of their whole transcriptome. Here, a method allowing for the isolation of SCs is presented, enabling the extraction and sequencing of RNAs from this rare cell population. The protocol consists of dissection, protease digestion and mechanical dissociation of the glands to obtain individual cells. Then, the cells are sorted by FACS, and living GFP-expressing SC singulets are isolated for RNA extraction. This procedure is able to provide SC-specific RNAs from ~40 males per condition in the course of one day. Given the speed and low number of flies required, this method enables the use of downstream RT-qPCR and/or RNA sequencing to the study gene expression in the SCs from different genetic backgrounds, ages, mating statuses or environmental conditions.SUMMARYHere, we describe the dissociation and sorting of a specific cell population from the Drosophila male accessory glands (Secondary cells), followed by RNA extraction for sequencing and RT-qPCR. The dissociation consists of dissection, proteases digestion and mechanical dispersion, followed by FACS purification of GFP-expressing cells.

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