Modulation of Adipocyte Differentiation and Proadipogenic Gene Expression by Sulforaphane, Genistein, and Docosahexaenoic Acid as a First Step to Counteract Obesity

Obesity is characterized by excess body fat accumulation due to an increase in the size and number of differentiated mature adipocytes. Adipocyte differentiation is regulated by genetic and environmental factors, and its inhibition could represent a strategy for obesity prevention and treatment. The current study was designed with two aims: (i) to evaluate the changes in the expression of adipogenic markers (C/EBPα, PPARγvariant 1 and variant 2, and GLUT4) in 3T3-L1 murine preadipocytes at four stages of the differentiation process and (ii) to compare the effectiveness of sulforaphane, genistein, and docosahexaenoic acid in reducing lipid accumulation and modulating C/EBPα, PPARγ1, PPARγ2, and GLUT4 mRNA expression in mature adipocytes. All bioactive compounds were shown to suppress adipocyte differentiation, although with different effectiveness. These results set the stage for further studies considering natural food constituents as important agents in preventing or treating obesity.

Download Full-text

Interaction of Genetic and Environmental Factors for Body Fat Mass Control

Case Medical Research ◽

10.31525/ct1-nct04299698 ◽

2020 ◽

Author(s):

Keyword(s):

Environmental Factors ◽

Body Fat ◽

Fat Mass ◽

Body Fat Mass ◽

Genetic And Environmental Factors

Download Full-text

Mechanism of increased PLD1 gene expression during early adipocyte differentiation process of mouse cell line 3T3-L1

Journal of Cellular Biochemistry ◽

10.1002/jcb.22414 ◽

2009 ◽

pp. n/a-n/a ◽

Cited By ~ 2

Author(s):

Siqiang Gao ◽

Hiromi Ito ◽

Masashi Murakami ◽

Kayo Yoshida ◽

Yoko Tagawa ◽

...

Keyword(s):

Gene Expression ◽

Cell Line ◽

Adipocyte Differentiation ◽

Mouse Cell ◽

Differentiation Process ◽

Mouse Cell Line

Download Full-text

Mechanisms and modifiers of methylmercury-induced neurotoxicity

Toxicology Research ◽

10.1039/c2tx20010d ◽

2012 ◽

Vol 1 (1) ◽

pp. 32-38 ◽

Cited By ~ 23

Author(s):

Stephanie J. B. Fretham ◽

Samuel Caito ◽

Ebany J. Martinez-Finley ◽

Michael Aschner

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Environmental Factors ◽

Genetic Polymorphisms ◽

Experimental Studies ◽

Mehg Exposure ◽

Complete Understanding ◽

Cellular Processes ◽

Gene Environment ◽

Genetic And Environmental Factors

Abstract The neurotoxic consequences of methylmercury (MeHg) exposure have long been known, however a complete understanding of the mechanisms underlying this toxicity is elusive. Recent epidemiological and experimental studies have provided mechanistic insights into the contribution of genetic and environmental factors that interact with MeHg to modify toxicity. This review will outline cellular processes directly and indirectly affected by MeHg, including oxidative stress, cellular signaling and gene expression, and discuss epigenetic modifications, genetic polymorphisms and gene–environment interactions capable of modifying MeHg neurotoxicity.

Download Full-text

Interaction of genetic and environmental factors for body fat mass control: observational study for lifestyle modification and genotyping

Scientific Reports ◽

10.1038/s41598-021-92229-5 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Joon Ho Kang ◽

Heewon Kim ◽

Jinki Kim ◽

Jong-Hwa Seo ◽

Soyeon Cha ◽

...

Keyword(s):

Environmental Factors ◽

Observational Study ◽

Body Fat ◽

Fat Mass ◽

Genetic Factors ◽

Lifestyle Modification ◽

Body Fat Mass ◽

Genetic Composition ◽

Lifestyle Modifications ◽

Genetic And Environmental Factors

AbstractPrevious studies suggested that genetic, environmental factors and their interactions could affect body fat mass (BFM). However, studies describing these effects were performed at a single time point in a population. In this study, we investigated the interaction between genetic and environmental factors in affecting BFM and implicate the healthcare utilization of lifestyle modifications from a personalized and genomic perspective. We examined how nutritional intake or physical activity changes in the individuals affect BFM concerning the genetic composition. We conducted an observational study including 259 adult participants with single nucleotide polymorphism (SNP) genotyping and longitudinal lifestyle monitoring, including food consumption and physical activities, by following lifestyle modification guidance. The participants’ lifelog data on exercise and diet were collected through a wearable device for 3 months. Moreover, we measured anthropometric and serologic markers to monitor their potential changes through lifestyle modification. We examined the influence of genetic composition on body fat reduction induced by lifestyle changes using genetic risk scores (GRSs) of three phenotypes: GRS-carbohydrate (GRS-C), GRS-fat (GRS-F), and GRS-exercise (GRS-E). Our results showed that lifestyle modifications affected BFM more significantly in the high GRS class compared to the low GRS class, indicating the role of genetic factors affecting the efficiency of the lifestyle modification-induced BFM changes. Interestingly, the influence of exercise modification in the low GRS class with active lifestyle change was lower than that in the high GRS class with inactive lifestyle change (P = 0.022), suggesting the implication of genetic factors for efficient body fat control.

Download Full-text

THE INTERACTION OF GENETIC AND ENVIRONMENTAL FACTORS IN THE CONTROL OF AMYLASE GENE EXPRESSION IN DROSOPHILA MELANOGASTER

Genetics ◽

10.1093/genetics/114.3.943 ◽

1986 ◽

Vol 114 (3) ◽

pp. 943-954

Author(s):

Bernhard F Benkel ◽

Donal A Hickey

Keyword(s):

Gene Expression ◽

Drosophila Melanogaster ◽

Environmental Factors ◽

Molecular Basis ◽

Amylase Activity ◽

Glucose Repression ◽

Amylase Gene ◽

Dietary Effects ◽

Eukaryotic Gene ◽

Genetic And Environmental Factors

ABSTRACT A number of previous studies have established that amylase activity can vary between Drosophila strains which are maintained under identical laboratory conditions. In addition, we have recently shown that all strains examined so far are subject to glucose repression of amylase activity. In this study, we show that the degree of glucose repression can vary between strains. Moreover, the glucose repression effect is much more pronounced in larvae than in adult flies. Our results lead to the conclusion that the strain-specific differences in activity and the dietary effects are not independent phenomena. These results have implications for the interpretation of many studies on amylase activity variation, including those experiments which have been designed to link amylase activity variations with fitness differences in nature. A question that naturally arises concerns the molecular basis for these strain-specific variations in the degree of glucose repression of this eukaryotic gene.

Download Full-text

Genetic and Environmental Factors Affecting Allergen-Related Gene Expression in Apple Fruit (Malus domestica L. Borkh)

Journal of Agricultural and Food Chemistry ◽

10.1021/jf800813d ◽

2008 ◽

Vol 56 (15) ◽

pp. 6707-6716 ◽

Cited By ~ 37

Author(s):

Alessandro Botton ◽

Paolo Lezzer ◽

Alberto Dorigoni ◽

Gianni Barcaccia ◽

Benedetto Ruperti ◽

...

Keyword(s):

Gene Expression ◽

Environmental Factors ◽

Malus Domestica ◽

Apple Fruit ◽

Related Gene ◽

Factors Affecting ◽

Genetic And Environmental Factors

Download Full-text

Body Fat Patterning Of Siblings From Mozambique. Genetic And Environmental Factors

Medicine & Science in Sports & Exercise ◽

10.1249/01.mss.0000402825.36429.7a ◽

2011 ◽

Vol 43 (Suppl 1) ◽

pp. 51

Author(s):

Antonio Prista ◽

Leonardo Nhantumbo ◽

Silvio Saranga ◽

Gaston Beunen ◽

José Maia

Keyword(s):

Environmental Factors ◽

Body Fat ◽

Genetic And Environmental Factors

Download Full-text

Global gene expression patterns spanning 3T3-L1 preadipocyte differentiation

Canadian Journal of Animal Science ◽

10.4141/a03-120 ◽

2004 ◽

Vol 84 (3) ◽

pp. 367-376 ◽

Cited By ~ 4

Author(s):

C. Hansen ◽

A. Fu ◽

C. Li ◽

W. T. Dixon ◽

R. Christopherson ◽

...

Keyword(s):

Gene Expression ◽

Time Course ◽

Adipocyte Differentiation ◽

Expression Patterns ◽

Subcutaneous Fat ◽

Global Gene Expression ◽

Differentiation Process ◽

Comprehensive Overview ◽

Self Organizing Maps ◽

Som Clustering

Adipogenesis is of significant relevance from an agricultural perspective. Traits such as subcutaneous fat thickness, marbling and waste fat are of substantial economic importance in animal production. In order to discover more about the genetic basis of this process, a study was undertaken to examine the changes that occur daily in global gene expression as 3T3-L1 cells differentiate from preadipocyte to adipocyte. Duplicate RNA samples were collected daily during the differentiation process and probed with the Affymetrix U74Av2 GeneChip® microarray to allow the time-course analysis of the gene expression profile in these differentiating cells. Self-organizing maps (SOM) clustering was performed to extract patterns of expression over the course of the experiment (day 0 to day 6). The clustering generated nine distinct expression patterns containing between 74 and 420 genes/ESTs. Functional clusters and important chronological changes in the expression of key genes and gene groups were identified. The pattern of expression observed for many genes not only confirmed what has been shown previously for the early stages of differentiation, but also expanded this pattern to cover the whole differentiation process thus giving a very comprehensive overview of patterns and changes in gene expression over the time course of adipocyte differentiation. Key words: Adipocyte differentiation, gene expression, SOM clustering

Download Full-text

Angiotensinogen gene expression in 3T3-L1 cells

AJP Cell Physiology ◽

10.1152/ajpcell.1989.256.2.c448 ◽

1989 ◽

Vol 256 (2) ◽

pp. C448-C451 ◽

Cited By ~ 75

Author(s):

J. A. Saye ◽

L. A. Cassis ◽

T. W. Sturgill ◽

K. R. Lynch ◽

M. J. Peach

Keyword(s):

Gene Expression ◽

Adipose Tissue ◽

Drug Treatment ◽

Time Course ◽

Adipocyte Differentiation ◽

Drug Regimen ◽

Mrna Levels ◽

Differentiation Process ◽

Clonal Cell Line ◽

Angiotensinogen Gene

It has previously been established that angiotensinogen mRNA is present in brown and white adipose tissue of the rat. To determine whether angiotensinogen gene expression is present in adipocytes as compared with other cell elements, we have examined angiotensinogen mRNA in 3T3-L1 cells. These cells undergo adipocyte differentiation when the culture reaches confluence. To accelerate the differentiation process, cells were treated with dexamethasone and isobutylmethylxanthine for 3 days. On the 7th day after drug treatment, RNA was extracted from cells and was examined for angiotensinogen mRNA using a full-length rat angiotensinogen cDNA. Angiotensinogen mRNA was readily detected in differentiated 3T3-L1 cells. To determine when the gene is expressed, a 7-day time course from day 0 (before drug treatment) to day 7 was examined for the presence of angiotensinogen mRNA. In addition, C2 cells, a clonal cell line that does not differentiate into adipocytes, were examined. Angiotensinogen mRNA was detected on days 2-7 after drug treatment in 3T3-L1 cells, with no detectable levels in untreated 3T3-C2 cells. When 3T3-C2 cells were subjected to the same drug regimen, angiotensinogen mRNA levels increased in the same time course as 3T3-L1 cells. However, the increase in angiotensinogen message was greater in differentiating 3T3-L1 cells than in the nondifferentiating 3T3-C2 cells. Thus angiotensinogen mRNA is present in both adipocytes and in fibroblast-like cells and appears to be regulated by steroids.

Download Full-text