scholarly journals EpCAMlow Circulating Tumor Cells: Gold in the Waste

2019 ◽  
Vol 2019 ◽  
pp. 1-5 ◽  
Author(s):  
Chiara Nicolazzo ◽  
Angela Gradilone ◽  
Flavia Loreni ◽  
Cristina Raimondi ◽  
Paola Gazzaniga
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Gold Standard ◽  
Cell Adhesion Molecule ◽  
Epithelial Cell Adhesion Molecule ◽  
Isolation And Characterization ◽  
Tumor Types

The CellSearch® system which is still considered the gold standard for the enumeration of circulating tumor cells (CTC) utilizes antibodies against the epithelial cell adhesion molecule (EpCAM) for CTC enrichment. Recently, CTC discarded by the CellSearch® system due to their low EpCAM expression have been isolated and analyzed. We here sought to discuss technical and biological issues concerning the isolation and characterization of EpCAMlow CTC, highlighting the enormous potential of this subpopulation discarded by CellSearch®, which might instead reveal an unexpected clinical significance in tumor types where CTC enumeration has never been validated for prognostic and predictive purpose.

Demasking of epithelial cell adhesion molecule (EpCAM) on circulating epithelial tumor cells by Tween®20 treatment in breast cancer patients

2012 ◽  
Vol 50 (4) ◽  
Author(s):  
Katya Hekimian ◽  
Ernst-Ludwig Stein ◽  
Ulrich Pachmann ◽  
Katharina Pachmann
Keyword(s):  
Breast Cancer ◽  
Immune Response ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cell Adhesion Molecule ◽  
Breast Cancer Patients ◽  
Epithelial Cell Adhesion Molecule ◽  
Epithelial Tumor

AbstractThe epithelial cell adhesion molecule (EpCAM) embedded in the plasma membrane of circulating epithelial tumor cells (CETC) is used for detection and enrichment of circulating tumor cells in peripheral blood and as a target for anti-epithelial antibodies elicited during immune response in anti-tumor immunization. Although an efficient immune response against EpCAM can be generated, the clinical application of such approaches has not been successful so far and the detection of circulating epithelial cells is highly variable. One reason for these discrepancies may be that not all circulating tumor cells are equally accessible for the specific antibody. A possible reason might be masking of EpCAM by glycoproteins or membrane lipoproteins preventing antibody binding.We have tested the application of detergents as demasking agents known to be successful in demasking red blood cell epitopes and determined how and in which way they affect integral membrane proteins and membrane lipids.The results showed that the polysorbate TweenThe data presented in this study suggest that EpCAM is present on part of circulating tumor cells in a masked form and that it is possible to demask EpCAM on CETC of breast cancer patients using Tween

scholarly journals Quick chip assay using locked nucleic acid modified epithelial cell adhesion molecule and nucleolin aptamers for the capture of circulating tumor cells

2015 ◽  
Vol 9 (5) ◽  
pp. 054110 ◽  
Author(s):  
Nihal G. Maremanda ◽  
Kislay Roy ◽  
Rupinder K. Kanwar ◽  
Vidyarani Shyamsundar ◽  
Vijayalakshmi Ramshankar ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Nucleic Acid ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Locked Nucleic Acid ◽  
Chip Assay ◽  
Epithelial Cell Adhesion Molecule

Epithelial Cell Adhesion Molecule-positive Circulating Tumor Cells as Predictive Biomarker in Patients With Prostate Cancer

Urology ◽  
2013 ◽  
Vol 81 (6) ◽  
pp. 1303-1307 ◽  
Author(s):  
Robert J. Amato ◽  
Vladislava Melnikova ◽  
Yujian Zhang ◽  
Wen Liu ◽  
Somyata Saxena ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Predictive Biomarker ◽  
Epithelial Cell Adhesion Molecule
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