scholarly journals Curcumin Provides Hepatoprotection against Amoebic Liver Abscess Induced by Entamoeba histolytica in Hamster: Involvement of Nrf2/HO-1 and NF-κB/IL-1β Signaling Pathways

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
José Roberto Macías-Pérez ◽  
Liseth Rubí Aldaba-Muruato ◽  
Sandra Luz Martínez-Hernández ◽  
Martín Humberto Muñoz-Ortega ◽  
Julieta Pulido-Ortega ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Liver Damage ◽  
Glycogen Content ◽  
Current Knowledge ◽  
The Other ◽  
Amoebic Liver Abscess ◽  
Normal Appearance ◽  
Hamster Model ◽  
First Time

Amoebic liver abscess (ALA) is the most common extraintestinal amoebiasis caused by Entamoeba histolytica (E. histolytica). However, despite current knowledge and scientific advances about this infection, there are no effective treatments to prevent it. Herein, the antiamoebic capacity of curcumin in a hamster model was evaluated. Curcumin (150 mg/kg, p.o., daily during 10 days before infection) considerably prevents liver damage induced at 12 and 48 h post-intrahepatic inoculation of trophozoites and decreases ALT, ALP, and γ-GTP activities, and macroscopic and microscopic observations were consistent with these results. On the other hand, after one week of intraportal inoculation, liver damage was prevented by curcumin (150 mg/kg, p.o., daily, 20 days before amoebic inoculation and during the week of infection); liver/body weight ratios and tissue and histological stains showed normal appearance; in addition, the increases in ALT, ALP, and γ-GTP activities were prevented; the depletion of glycogen content induced by the amoebic damage was partially but significantly prevented, while NF-κB activity was inhibited and the expression of IL-1β was reduced; Nrf2 production showed a tendency to increase it, and HO-1 protein was overexpressed. These results suggest for the first time that curcumin can be a compound with antiamoebic effect in the liver, suggesting that its daily use could help greatly decrease the incidence of this type of infection.

scholarly journals Entamoeba histolytica acetyl–CoA synthetase: biomarker of acute amoebic liver abscess

2014 ◽  
Vol 4 (6) ◽  
pp. 446-450
Author(s):  
Lim Boon Huat ◽  
Alfonso Olivos Garcia ◽  
Tan Zi Ning ◽  
Wong Weng Kin ◽  
Rahmah Noordin ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Amoebic Liver Abscess ◽  
Acetyl Coa

Linearolactone and Kaempferol Disrupt the Actin Cytoskeleton in Entamoeba histolytica: Inhibition of Amoebic Liver Abscess Development

2020 ◽  
Vol 83 (12) ◽  
pp. 3671-3680
Author(s):  
José Antonio Velázquez-Domínguez ◽  
Verónica Ivonne Hernández-Ramírez ◽  
Fernando Calzada ◽  
Luis Varela-Rodríguez ◽  
Diana L. Pichardo-Hernández ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Amoebic Liver Abscess

Entamoeba histolytica antigenic protein detected in pus aspirates from patients with amoebic liver abscess

2013 ◽  
Vol 134 (4) ◽  
pp. 504-510 ◽  
Author(s):  
Nurulhasanah Othman ◽  
Zeehaida Mohamed ◽  
Maya Mazuwin Yahya ◽  
Voon Meng Leow ◽  
Boon Huat Lim ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Amoebic Liver Abscess ◽  
Antigenic Protein

scholarly journals Protection against Invasive Amebiasis by a Single Monoclonal Antibody Directed against a Lipophosphoglycan Antigen Localized on the Surface of Entamoeba histolytica

1997 ◽  
Vol 186 (9) ◽  
pp. 1557-1565 ◽  
Author(s):  
Alexandra Marinets ◽  
Tonghai Zhang ◽  
Nancy Guillén ◽  
Pierre Gounon ◽  
Barbara Bohle ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Passive Immunization ◽  
Outer Face ◽  
Protective Capacity ◽  
Con A ◽  
Entamoeba Dispar ◽  
Confocal Immunofluorescence ◽  
Scid Mouse Model ◽  
First Time

A panel of monoclonal antibodies was raised from mice immunized with a membrane preparation from Entamoeba histolytica, the pathogenic species causing invasive amebiasis in humans. Antibody EH5 gave a polydisperse band in immunoblots from membrane preparations from different E. histolytica strains, and a much weaker signal from two strains of the nonpathogenic species Entamoeba dispar. Although the exact chemical structure of the EH5 antigen is not yet known, the ability of the antigen to be metabolically radiolabeled with [32P]phosphate or [3H]glucose, its sensitivity to digestion by mild acid and phosphatidylinositol-specific phospholipase C, and its specific extraction from E. histolytica trophozoites by a method used to prepare lipophosphoglycans from Leishmania showed that it could be classified as an amebal lipophosphoglycan. Confocal immunofluorescence and immunogold labeling of trophozoites localized the antigen on the outer face of the plasma membrane and on the inner face of internal vesicle membranes. Antibody EH5 strongly agglutinated amebas in a similar way to concanavalin A (Con A), and Con A bound to immunoaffinity-purified EH5 antigen. Therefore, surface lipophosphoglycans may play an important role in the preferential agglutination of pathogenic amebas by Con A. The protective ability of antibody EH5 was tested in a passive immunization experiment in a severe combined immunodeficient (SCID) mouse model. Intrahepatic challenge of animals after administration of an isotype-matched control antibody or without treatment led to the development of a liver abscess in all cases, whereas 11 out of 12 animals immunized with the EH5 antibody developed no liver abscess. Our results demonstrate the importance and, for the first time, the protective capacity of glycan antigens on the surface of the amebas.

scholarly journals Rapid diagnosis of amoebic liver abscess using recombinant Entamoeba histolytica pyruvate phosphate dikinase

2014 ◽  
Vol 4 (3) ◽  
pp. 225
Author(s):  
S. Syazwan ◽  
Hafiznur Y. Muhammad ◽  
Dyana Z. Nor ◽  
A.R. Khairunisak ◽  
B.H. Lim ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Rapid Diagnosis ◽  
Amoebic Liver Abscess ◽  
Pyruvate Phosphate Dikinase

scholarly journals Pulmonary Thromboembolism Complicating Amebic Liver Abscess: First Reported Case in the United States—Case Report and Literature Review

2015 ◽  
Vol 2015 ◽  
pp. 1-3 ◽  
Author(s):  
Devon McKenzie ◽  
Michael Gale ◽  
Sunny Patel ◽  
Grazyna Kaluta
Keyword(s):  
Inferior Vena Cava ◽  
Entamoeba Histolytica ◽  
Liver Abscess ◽  
Inferior Vena ◽  
Pulmonary Thromboembolism ◽  
Current Knowledge ◽  
Vena Cava ◽  
The United States ◽  
Amebic Liver Abscess ◽  
The Usa

Even in the absence of Amebic colitis, Amebic liver abscess (ALA) is the most common extraintestinal complication ofEntamoeba histolyticainfection. In the USA, it is most prevalent in middle aged immigrant males from endemic countries such as Africa, Mexico, and India. One of the complications of ALA is inferior vena cava (IVC) thrombosis, which is believed to result from the mechanical compression of the IVC and the consequent thrombogenic nidus elicited from the resultant inflammatory response. There are very few reported cases and even fewer in which the thrombus became a harbinger to pulmonary thromboembolism. We present the case of a 43-year-old male from West Africa who presented with the chief complaint of right upper quadrant abdominal pain for one week associated with persistent nonproductive cough. He had a positive serumEntamoeba histolyticaantibody with CT scan findings of a hepatic abscess with thrombosis of the hepatic vein and inferior vena cava and numerous bilateral pulmonary emboli. This amebic liver abscess was successfully treated with metronidazole and paromomycin, whereas the pulmonary thromboembolism was managed with medical anticoagulation. Based on current knowledge, this is the first reported case in the USA.

scholarly journals Spinnerets and silk-producing system of Segestria senoculata (Araneae, Araneomorphae, Segestriidae)

2016 ◽  
Vol 48 (3) ◽  
pp. 388 ◽  
Author(s):  
S. Karschová ◽  
J. Hajer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Current Knowledge ◽  
The Other ◽  
Sem Study ◽  
First Time ◽  
Scanning Electron

The spinning apparatus and silk of <em>Segestria senoculata</em> were studied with the use of scanning electron microscopy and light microscopy, which confirmed the presence of four categories of spigots connected with four types of spinning glands (<em>i.e</em>., <em>Glandulae ampullaceae major, Glandulae ampullaceae minor, Glandulae piriformes and Glandulae pseudaciniformes</em>). New data about the morphology of spinnerets and spigots were obtained for both the adults and nymphal stages of both sexes. For the first time the silken threads of retreats, signal threads and attachment discs of the members of <em>Segestria</em> were subjected to a detailed SEM study. The data resulting from studying the spinning apparatus of <em>S. senoculata</em> was compared to current knowledge of the silk producing systems of families belonging to the Dysderoidea superfamily. Silks that are emitted from spigots in the course of retreat construction are not (similarly to the other dysderoids) processed by the spider’s legs during the subsequent process of hardening. Apart from the major ampullate glands/spigots, segestriids also possess developed minor ampullate glands. Minor ampullate threads are used by <em>S. senoculata</em> spiders when making their signal threads.

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