scholarly journals The Traditional Chinese Medicine Fufang Shatai Heji (STHJ) Enhances Immune Function in Cyclophosphamide-Treated Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Kai-Jian Fan ◽  
Yun-Wu Li ◽  
Jing Wu ◽  
Jun Li ◽  
Jun Zhang ◽  
...  
Keyword(s):  
Immune Function ◽  
Lymphocyte Subsets ◽  
Nk Cell ◽  
Primary Objective ◽  
Inflammatory Factors ◽  
Traditional Chinese Medicines ◽  
Radix Astragali ◽  
Oral Gavage ◽  
Chinese Medicines ◽  
Necrosis Factor

Fufang Shatai Heji (STHJ) is a mixture of traditional Chinese medicines, such as Radix Adenophorae, Radix Pseudostellariae, and Radix Astragali. STHJ is commonly used to treat diseases caused by low immune function, for example, Sjögren’s syndrome (SS). The primary objective of this study was to assess the immunopotentiating effect of STHJ using an immunosuppressive mouse model receiving cyclophosphamide (CTX). Following CTX treatment, STHJ was administered by oral gavage for 30 consecutive days. The percentage of specific lymphocyte subpopulations in the spleen was measured by flow cytometry. Levels of inflammatory factors in serum were detected by enzyme-linked immunosorbent assays (ELISAs). The administration of STHJ significantly elevated thymus and spleen indices, increased B cell and natural killer (NK) cell activities, and decreased CD8+ T, CD8+CD122+ T, NKT, and γδT cell activities in the CTX-treated mice. In addition, STHJ upregulated the expression of interleukin- (IL-) 2, IL-6, and tumor necrosis factor-α (TNF-α) and downregulated IL-10 expression in CTX-treated mice. In conclusion, STHJ effectively remitted CTX-induced immunosuppression by modulating the balance of lymphocyte subsets and cytokines. Our results suggest STHJ treatment could be used as an effective therapeutic approach to improve immune function in patients with low immunity.

scholarly journals Effects of Two Polysaccharides From Traditional Chinese Medicines on Rat Immune Function

2021 ◽  
Vol 8 ◽  
Author(s):  
Yu Lei Wang ◽  
Xiang Hua Shu ◽  
Xue Zhang ◽  
Yong Bo Liu ◽  
Ya Jing Zhang ◽  
...  
Keyword(s):  
Immune Function ◽  
Stimulation Index ◽  
White Blood Cells ◽  
The Body ◽  
Raw Material ◽  
Chinese Herbs ◽  
Traditional Chinese Medicines ◽  
Sprague Dawley ◽  
Chinese Medicines ◽  
Spleen Index

Abstract: The aim is to study the immune function effect of two polysaccharides extracted from traditional Chinese herbs on rats. Ultrasonic-assisted extraction was used to extract the polysaccharide from traditional Chinese medicines. MTT assay was used to determine the effects of two polysaccharides on the conversion of pig peripheral T lymphocytes. For this, 24 Sprague–Dawley rats were selected for the clinical trial and divided into groups B (blank), CK (cyclophosphamide inhibitory control), AP (angelica polysaccharide), and RIP (radix isatidis polysaccharide). Except for group B, other groups can induce the immunodeficiency by using cyclophosphamide. Rats of the AP and RIP groups were given gavage of 1 mL of AP and RIP. The blood was sampled from the eyeball on days 0, 7, 14, 21, 28, and 35, respectively, to determine immune cells, IgG and IgM of immunoglobulin, body weight, and spleen index.Results: The average content of AP and RIP was 51.27 and 14.8%, and the extraction rate was 75.23 and 60.94%. The maximum stimulation index was 1.407 when the concentration of AP was 8,000 μg mL−1 and 1.5 when the concentration of RIP was 125 μg mL−1. Both kinds of polysaccharides can alleviate the decline of white blood cells, lymphocytes, monocytes, neutrophils, and serum IgG and IgM caused by cyclophosphamide. The two polysaccharides can regulate the rapid recovery of weight in immunosuppressed rats and increase the spleen index of immunosuppressed SD rats. The polysaccharides from the two traditional Chinese medicines can alleviate the immunosuppression caused by cyclophosphamide and promote the immune function of the body, which can be used as raw material resources of new veterinary medicine.

Tumor Necrosis Factor and Traditional Chinese Medicines

2015 ◽  
pp. 223-233 ◽  
Author(s):  
Katsuyuki Haranaka ◽  
Nobuko Satomi ◽  
Akiko Sakurai ◽  
Ruriko Haranaka
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Traditional Chinese Medicines ◽  
Chinese Medicines ◽  
Necrosis Factor

Antitumor activities and tumor necrosis factor producibility of traditional Chinese medicines and crude drugs

1985 ◽  
Vol 20 (1) ◽  
Author(s):  
Katsuyuki Haranaka ◽  
Nobuko Satomi ◽  
Akiko Sakurai ◽  
Ruriko Haranaka ◽  
Naoko Okada ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Traditional Chinese Medicines ◽  
Antitumor Activities ◽  
Chinese Medicines ◽  
Crude Drugs ◽  
Necrosis Factor

Inhibitory Effects of Anti-SARS Traditional Chinese Medicines on the UV Irradiation of λ-Lysogen

2006 ◽  
Vol 34 (01) ◽  
pp. 147-155 ◽  
Author(s):  
Yaqiong Li ◽  
Jing Li ◽  
Chengxiang Fang
Keyword(s):  
Electron Spin Resonance ◽  
Uv Irradiation ◽  
Medicinal Herbs ◽  
Traditional Chinese Medicines ◽  
Radix Astragali ◽  
Inhibitory Effects ◽  
Chinese Medicines ◽  
Radix Glycyrrhizae ◽  
Spin Resonance ◽  
Uv Induction

By using λ-lysogen as a model, the inhibitory effects of anti-severe acute respiratory syndrome (SARS) traditional Chinese medicines (TCMs) prescription I on the UV irradiation were investigated in this present study. It was found that the prescription I possessed obvious inhibitory effects on the UV induction of λ-lysogen, the inhibitory rate reaching 83.87%. Among five medicinal herbs prescribed in that formula, Herba Patriniae, Radix Astragali and Radix Glycyrrhizae played important roles. When these three herbs were eliminated from the recipe separately, the inhibitory effects were prominently decreased. If only one of these five medicinal herbs was added into the medium of λ-lysogen, the inhibitory rates ranged from 27.0% ~ 45.0%. By electron spin resonance (ESR) detection, we found that the prescription I , Herba Patriniae and other main herbs in that recipe, could quench effectively the free radicals generated in the process of λ-lysogenic cells by UV. These results provide a novel idea for further studying the pharmacology of TCM and exploring the mechanism of SARS virus infection.

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