scholarly journals Characteristics and Epidemiological Investigation of Paratuberculosis in Dairy Cattle in Tai’an, China

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Zilong Cheng ◽  
Mengda Liu ◽  
Peng Wang ◽  
Peng Liu ◽  
Meng Chen ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Clinical Symptoms ◽  
Shandong Province ◽  
Chain Reaction ◽  
Serum Samples ◽  
Fatal Disease ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Subcutaneous Edema ◽  
Polymerase Chain

Paratuberculosis, a chronic and sometimes fatal disease of ruminants, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, we examined paratuberculosis cases among 2–4-year-old dairy cows at farms in Shandong Province, China. Paratuberculosis cases were diagnosed based on clinical symptoms, pathological autopsy, and histopathological inspection. Characteristics of paratuberculosis in the affected dairy cattle included poor body condition, persistent diarrhea, subcutaneous edema, granulomatous ileitis (multibacillary), mesenteric lymphadenitis, and hepatitis. Acid-fast bacilli from fecal specimens and lymphocytes were putatively identified as MAP based on Ziehl-Neelsen staining, then confirmed using polymerase chain reaction-based testing and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analyses. Overall, only one MAP strain was isolated from a herd with symptomatic diarrhea. However, analysis of 586 serum samples from nine herds in Tai’an City revealed that 66.7% of herds and 14.2% of animals were seropositive for MAP. Our findings suggest that paratuberculosis is widely prevalent and therefore a significant threat to the dairy industry in Tai’an City, Shandong Province, China.

scholarly journals Detecção de microrganismos em dispositivos ortopédicos sonicados clínicos usando cultura convencional e qPCR

2021 ◽  
Author(s):  
Victoria Stadler Tasca Ribeiro ◽  
Juliette Cieslinski ◽  
Julia Bertol ◽  
Ana Laura Schumacher ◽  
João Paulo Telles ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Chain Reaction ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Polymerase Chain ◽  
Tof Ms ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Resumo Objetivo Avaliar a sensibilidade e a especificidade da reação em cadeia de polimerase em tempo real quantitativa (quantitative real-time polymerase chain reaction, qPCR, em inglês) para a triagem do gene rDNA 16S, com a utilização do fluido sonicado de implantes ortopédicos. Métodos Um estudo retrospectivo foi realizado em 73 fluidos sonicados obtidos de pacientes com infecção associada aos implantes ortopédicos. As amostras foram submetidas a cultura convencional e a teste molecular utilizando ionização e dessorção a laser assistida por matriz com espectrometria de massa por tempo de voo (matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF MS, em inglês) e qPCR para o gene rDNA 16S. Os valores limiares do ciclo foram usados para definir um ponto de corte para a qPCR do gene rDNA 16S para culturas negativas e positivas. Resultados Não foram observadas diferenças estatísticas entre os grupos de cultura positiva e negativa com base no tempo desde a primeira cirurgia até a infecção (p = 0,958), na idade (p = 0,269), ou nas comorbidades em geral. No entanto, uma diferença estatística foi encontrada entre a duração média do uso de antibióticos antes da remoção do dispositivo (3,41 versus 0,94; p = 0,016). O DNA bacteriano foi identificado em todas as amostras dos fluidos sonicados. Os limiares do ciclo médio de culturas positivas e negativas foram de 25,6 e 27,3, respectivamente (p < 0,001). Como uma ferramenta de diagnóstico, um corte do limite do ciclo de 26,89 demonstrou uma área sob a curva da característica de operação do receptor de 0,877 (p ≤ 0,001). Conclusão A presença de agentes antimicrobianos por mais de 72 horas diminuiu a positividade da cultura, mas não influenciou os resultados da qPCR. Apesar disso, a amplificação do rDNA 16S pode sobrestimar o diagnóstico de infecção.

scholarly journals Isolation of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus from nature: Technological characterisation and antibiotic resistance

2021 ◽  
Author(s):  
Hakan Tavşanlı ◽  
Tülay Elal Mus ◽  
Figen Cetinkaya ◽  
Ergün Aynaoglu ◽  
Recept Cibik
Keyword(s):  
Skim Milk ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Delbrueckii ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Ionization Time ◽  
Flight Mass Spectrometry ◽  
Fermenting Bacteria ◽  
Polymerase Chain ◽  
Lactobacillus Delbrueckii Subsp

Yoghurt fermenting bacteria were isolated from natural sources including plants, dew, and rain samples (total of 300 samples) by the same methods nomadic peoples used for several centuries in Turkey. Inoculation into the reconstituted skim milk followed by planting on specific media and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) analysis allowed for the identification of 18 Lactobacillus delbrueckii subsp. and 26 Streptococcus thermophilus. A multiplex polymerase chain reaction (PCR) assay applied to lactobacilli enabled the identification of 5 isolates as L. delbrueckii subsp. bulgaricus. The isolates showed a varying range of acidification rates and proteolytic activity in reconstituted skimmed milk (RSM). S. thermophilus isolates showed a broader range of resistance and the most frequent resistance was observed for streptomycin (69.2%), gentamycin (65.3%), clindamycin (61.5%), ampicillin (61.5%), kanamycin (53.8%), and erythromycin (50%). For L. delbrueckii subsp. the highest resistance was determined for vancomycin (38.8%), ciprofloxacin (33.3%), and penicillin (27.8%). The frequency of multiple resistance was tested on 14 different antimicrobials determining that 19 S. thermophilus (73%) and 3 L. delbrueckii subsp. (16.7%) demonstrated resistance to more than three different antibiotics. In contrast to this wide-ranging resistance, five isolates from each genus were found to be susceptible to all tested antibiotics. The present study indicates that lactic acid bacteria (LAB) isolated from nature may have broad-range of resistance to antibiotics and could be a source for the transfer of resistance.

scholarly journals Streptococcus mutans detection on mother-child pairs using matrix-assisted laser desorption ionization – time of flight mass spectrometry and polymerase chain reaction

2021 ◽  
Vol 54 (1) ◽  
pp. 52
Author(s):  
Udijanto Tedjosasongko ◽  
Dwi Mulia Ramadhaniati ◽  
Seno Pradopo
Keyword(s):  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Chain Reaction ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Two Samples ◽  
Polymerase Chain ◽  
Tof Ms

Background: Streptococcus mutans (S. mutans) bacteria mainly cause dental caries in children. These bacteria are not considered oral indigenous bacteria since they are transmitted from people around children during their deciduous teeth eruption. The detection of these bacteria can be used for dental caries prevention in children. Purpose: To determine the strain and serotype of S. mutans by using matrix assisted laser desorption ionization – time of flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction (PCR) on dental plaque samples taken from mother-child pairs. Methods: Sixteen dental plaque samples of mother-child pairs were cultured on brain heart infusion broth (BHIB) and mitis salivarius bacitracin (MSB) media until S. mutans colony isolates were obtained. Next, the isolates of S. mutans colony were introduced into the target plates of MALDI-TOF MS, and then ionized to become peptide mass fingerprint (PMF). Afterwards, the colony isolates were detected by database software. The detected S. mutans DNA then was extracted by using conventional 727 bp PCR (serotype C). Results: Six strains of S. mutans were detected by MALDI-TOF MS method. Five samples were classified into UA159, two samples were 3SN1, two samples were NFSM1, two samples were 11A1, two samples were U138, two samples were 4SM1, and one sample was classified into another bacterium. Five out of 16 samples were detected by PCR as serotype C (UA159). Conclusion: Six strains of S. mutans were detected, namely UA159, 3SN1, NFSM1, 11A1, U138, and 4SM1, one of them (UA159) was detected as serotype C.

scholarly journals Serum lnc34a is a potential prediction biomarker for bone metastasis in hepatocellular carcinoma patients

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Li Zhang ◽  
Hao Niu ◽  
Ping Yang ◽  
Jie Ma ◽  
Bao-Ying Yuan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Bone Metastasis ◽  
Univariate Analysis ◽  
High Serum ◽  
Chain Reaction ◽  
Serum Samples ◽  
Early Screening ◽  
Expression Levels ◽  
Node Metastasis ◽  
Polymerase Chain

Abstract Background Early screening and intervention therapies are crucial to improve the prognosis of hepatocellular carcinoma (HCC) patients with bone metastasis. We aimed to identify serum lncRNA as a prediction biomarker in HCC bone metastasis. Methods The expression levels of lnc34a in serum samples from 157 HCC patients were detected by quantitative real-time polymerase chain reaction (PCR). Univariate analysis and multivariate analysis were performed to determine statistically significant variables. Results Expression levels of lnc34a in serum from HCC patients with bone metastasis were significantly higher than those without bone metastasis. The high expressions of lnc34a, vascular invasion and Barcelona Clinic Liver Cancer (BCLC) stage were associated with bone metastasis by analysis. Moreover, lnc34a expression was specifically associated with bone metastasis rather than lung or lymph node metastasis in HCC. Conclusions High serum lnc34a expression was a independent risk factor for developing bone metastasis in HCC.

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