scholarly journals METTL3 Intensifies the Progress of Oral Squamous Cell Carcinoma via Modulating the m6A Amount of PRMT5 and PD-L1

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Yilong Ai ◽  
Shiwei Liu ◽  
Hailing Luo ◽  
Siyuan Wu ◽  
Haigang Wei ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Mrna Translation ◽  
Qrt Pcr ◽  
Methylation Assay ◽  
Eukaryotic Mrnas ◽  
M6a Rna Methylation

N6-Methyladenosine (m6A) modification is one of the commonest chemical modifications in eukaryotic mRNAs, which has essential effects on mRNA translation, splicing, and stability. Currently, there is a rising concern on the regulatory role of m6A in tumorigenesis. As a known component in the m6A methyltransferase complex, METTL3 (methyltransferase-like 3) plays an essential role in m6A methylation. Till now, the functions of METTL3 in oral squamous cell carcinoma (OSCC) and its relative mechanism remain to be explored. In this research, through the GEPIA database, we found that high METTL3 expression has a correlation with poor prognosis of squamous cell carcinoma of head and neck. qRT-PCR displayed that METTL3 was highly expressed in OSCC cells. Functionally, METTL3 knockdown reduced the invasion, migration, and proliferation competence of OSCC cells and attenuated the activation of CD8+ T cells. In contrast, METTL3 overexpression resulted in opposite results. GEPIA, UALCAN, and SRAMP databases, PCR, western blot, and m6A RNA methylation assay confirmed the m6A modification of PRMT5 and PD-L1 mediated by METTL3. In conclusion, our results displayed that METTL3 intensified the metastasis and proliferation of OSCC by modulating the m6A amounts of PRMT5 and PD-L1, suggesting that METTL3 may be a therapeutic target for OSCC patients.

scholarly journals Expression Profile of Circular RNAs in Oral Squamous Cell Carcinoma

2020 ◽  
Vol 10 ◽  
Author(s):  
Yanxiong Shao ◽  
Yuhan Song ◽  
Siming Xu ◽  
Siyi Li ◽  
Haiwen Zhou
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Expression Profile ◽  
Squamous Cell ◽  
Potential Role ◽  
Inhibitory Effect ◽  
Circular Rnas ◽  
Qrt Pcr

BackgroundCircular RNAs (circRNAs) are involved in the pathogenesis of several diseases. Among oral maxillofacial cancers, oral squamous cell carcinoma (OSCC) has the highest incidence. However, the role of circRNAs in OSCC is still not clear. The aim of our study was to evaluate the circRNA expression profile in OSCC and explore further the potential role of circRNAs in the pathogenesis of OSCC.MethodsCircRNA sequencing was performed in 6 pairs of samples of OSCC and normal oral mucosal tissues. Expression of selected circRNAs was validated by qRT-PCR. GO and KEGG analyses were performed and binding relationships between circRNAs and miRNAs were predicted. The hsa_circ_0001766/miR-877-3p/VEGFA axis was selected to further elucidate its role in OSCC.ResultsWe showed that there were 122 differentially expressed (DE) circRNAs. Eight out of 10 selected circRNAs were validated by qRT-PCR. GO and KEGG analyses indicated that the identified DE circRNAs might be involved in the progression of OSCC. Then, after identification by Sanger sequencing and RNase R assay, the upregulated hsa_circ_0001766 was selected to investigate its potential role in OSCC. Bioinformatics analysis showed that hsa_circ_0001766 might act as a competing endogenous RNA (ceRNA) that sponged miR-877-3p to upregulate VEGFA expression. We selected OSCC cell lines SCC9 and SCC25. PCR results showed that the expression of hsa_circ_0001766 and VEGFA was upregulated in SCC9 and SCC25. Subsequently, using western blot, PCR, CCK8, and colony formation assays, we found that knocking down circRNA0001766 inhibited the expression of VEGFA and the proliferation of OSCC cells. Following this, miR-877-3p inhibitor reversed the inhibitory effect of si-hsa_circ_0001766 on expression of VEGFA and proliferation of OSCC cells.ConclusionsIn conclusion, our study revealed the possible role of circRNAs in the pathogenesis of OSCC, and identified the potential role of the hsa_circ_0001766/miR-877-3p/VEGFA axis in OSCC progression.

Rediscussing the Role of Traditional Risk Factors in Young Adults With Oral Squamous Cell Carcinoma

2020 ◽  
Vol 40 (12) ◽  
pp. 6987-6995
Author(s):  
NORBERT NECKEL ◽  
MARCO MICHAEL ◽  
DANIEL TROELTZSCH ◽  
JONAS WÜSTER ◽  
STEFFEN KOERDT ◽  
...  
Keyword(s):  
Risk Factors ◽  
Squamous Cell Carcinoma ◽  
Young Adults ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Traditional Risk Factors

The role of human papillomavirus in oral squamous cell carcinoma: myth and reality

2012 ◽  
Vol 18 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Katinka Kansy ◽  
Oliver Thiele ◽  
Kolja Freier
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell

Protumor role of estrogen receptor expression in oral squamous cell carcinoma cells

2021 ◽  
Author(s):  
Rie Akyu (Takei) ◽  
Kei Tomihara ◽  
Manabu Yamazaki ◽  
Rohan Moniruzzaman ◽  
Wataru Heshiki ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Estrogen Receptor ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Carcinoma Cells ◽  
Receptor Expression ◽  
Estrogen Receptor Expression

scholarly journals The role of chemotherapy and operation on lymphocytes accumulation in peripheral blood obtained from patients with oral squamous cell carcinoma

SpringerPlus ◽  
2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Tao Yu ◽  
Ping Guo ◽  
Yingying Wu ◽  
Jiafeng Zhao ◽  
Lichun Wu ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Peripheral Blood

scholarly journals Role of viruses in oral squamous cell carcinoma

2012 ◽  
Vol 6 (2) ◽  
pp. 21 ◽  
Author(s):  
Rashmi Metgud ◽  
Madhusudan Astekar ◽  
Meenal Verma ◽  
Ashish Sharma
Keyword(s):  
Risk Factors ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Genetic Susceptibility ◽  
Squamous Cell ◽  
Oncogenic Viruses ◽  
Exact Role ◽  
Carcinogenic Effects

The etiology of oral squamous cell carcinoma (OSCC) is complex and involves many factors. The most clearly defined risk factors are smoking and alcohol, which substantially increase the risk of oral SCC. However, despite this clear association, a substantial proportion of patients develop OSCC without exposure to them, emphasizing the role of other risk factors such as genetic susceptibility and oncogenic viruses. Some viruses are strongly associated with OSCC while the association of others is less frequent and may depend on co-factors for their carcinogenic effects. Therefore, the exact role of viruses must be evaluated with care in order to improve the diagnosis and treatment of OSCC.

Oral squamous cell carcinoma: Key clinical questions, biomarker discovery, and the role of proteomics

2016 ◽  
Vol 63 ◽  
pp. 53-65 ◽  
Author(s):  
Uzma Urooj Malik ◽  
Shamshad Zarina ◽  
Stephen R. Pennington
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Biomarker Discovery ◽  
Clinical Questions

Role of theMIR146Apolymorphism in the origin and progression of oral squamous cell carcinoma

2014 ◽  
Vol 122 (3) ◽  
pp. 198-201 ◽  
Author(s):  
Annalisa Palmieri ◽  
Francesco Carinci ◽  
Marcella Martinelli ◽  
Furio Pezzetti ◽  
Ambra Girardi ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell

Role of PET Scan in Initial Staging of Oral Squamous Cell Carcinoma

2014 ◽  
Vol 72 (9) ◽  
pp. e161
Author(s):  
D. Steel ◽  
M. Kalayeh ◽  
S. McClure
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Pet Scan ◽  
Initial Staging
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