The Synergistic Beneficial Effect of Thyme Honey and Olive Oil against Diabetes and Its Complications Induced by Alloxan in Wistar Rats

Diabetes is a metabolic disorder characterized by a chronic increase in blood glucose. Owing to the limitations observed with antidiabetics in modern medicine, medicinal plants and bee products are known as good matrices for the search for new antidiabetic molecules. The present study focused on the evaluation of the hypoglycemic and the protective properties of two natural products widely used in complementary and alternative medicine (thyme honey and olive oil). To achieve this, the study was carried out on Wistar rats rendered diabetic by the injection of a single dose of alloxan monohydrate (65 mg/kg body weight (BW)). First, the physicochemical characterization and the phytochemical analysis of thyme honey and olive oil were carried out, and then in vivo study was conducted on 42 Wistar rats divided into seven groups: three groups were normal, one group was untreated diabetic, and three groups were diabetic rats treated with thyme honey (2 g/kg BW) or olive oil (10 mL/kg BW) or their combination ((1 g/kg BW of thyme honey) and (5 mL/kg BW of olive oil)). During the experiment, the glycemia was measured regularly every 10 days. After 30 days of treatment, the rats were sacrificed. The serum and urine were analyzed to determine hepatic enzymes levels (AST, ALT, ALP, and LDH), lipidic profile (total cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein), and kidney parameters (urea, uric acid, creatinine, total protein, sodium, potassium, and chloride). The liver, pancreas, and kidneys were analyzed to evaluate their histological changes and to determine their enzymatic antioxidant content (catalase, GSH, and GPx) and the levels of MDA. The results obtained showed that thyme honey or olive oil, and especially their combination, improved significantly the blood glucose levels and they protect against metabolic changes and the complications induced by diabetes.

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Effect of Honey & Olive Oil Supplemented Bio-Yoghurt Feeding on Lipid Profile, Blood Glucose and Hematological Parameters in Rats

Current Nutrition & Food Science ◽

10.2174/1573401313666170905160124 ◽

2019 ◽

Vol 15 (2) ◽

pp. 140-147

Author(s):

Magdy M. Ismail ◽

El-Tahra M. Ammar ◽

Abd El-Wahab E. Khalil ◽

Mohamed Z. Eid

Keyword(s):

Body Weight ◽

Weight Gain ◽

Blood Glucose ◽

Olive Oil ◽

Body Weight Gain ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Lipoprotein Cholesterol ◽

Low Density ◽

Low Density Lipoprotein Cholesterol

Background and Objective: Yoghurt, especially bio-yoghurt has long been recognized as a product with many health benefits for consumers. Also, honey and olive oil have considerable nutritional and health effects. So, the effect of administration of yoghurt made using ABT culture and fortified with honey (2 and 6%), olive oil (1 and 4%) or honey + olive oil (2+1 and 6+4% respectively) on some biological and hematological properties of rats was investigated.Methods:The body weight gain, serum lipid level, blood glucose level, serum creatinine level, Glutamic Oxaloacetic Transaminase (GOT) activity, Glutamic Pyruvic Transaminase (GPT) activity, leukocytes and lymphocytes counts of rats were evaluated.Results:Blending of bio-yoghurt with rats' diet improved body weight gain. Concentrations of Total plasma Cholesterol (TC), High-Density Lipoprotein cholesterol (HDL), Low-Density Lipoprotein cholesterol (LDL), Very Low-Density Lipoprotein cholesterol (VLDL) and Triglycerides (TG) significantly lowered in plasma of rats fed bio-yoghurt. Levels of TC, LDL, VLDL, and TG also decreased in rat groups feed bio-yoghurt supplemented with honey and olive oil. LDL concentrations were reduced by 10.32, 18.51, 34.17, 22.48, 43.30% in plasma of rats fed classic starter yoghurt, ABT yoghurt, ABT yoghurt contained 6% honey, ABT yoghurt contained 4% olive oil and ABT yoghurt contained 6% honey + 4% olive oil respectively. The blood glucose, serum creatinine, GOT and GPT values of rats decreased while white blood cells and lymphocytes counts increased by feeding bioyoghurt contained honey and olive oil.Conclusion:The findings enhanced the multiple therapeutic effects of bio-yoghurt supplemented with honey and olive oil.

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The Hypoglycaemic, Antihyperlipidemic and Antioxidative Effects of Anacardium Occidentale Methanolic Nut Extract in Streptozotocin-Induced Diabetic Male Wistar Rats

10.21203/rs.3.rs-281201/v1 ◽

2021 ◽

Author(s):

Folasade O. AJAO ◽

Michael A. Olamoyegun ◽

Marcus O. Iyedupe

Keyword(s):

Body Weight ◽

Blood Glucose ◽

Wistar Rats ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Anacardium Occidentale ◽

Low Density ◽

Reference Drug ◽

Antioxidative Effects ◽

Group 2

Abstract Background: This research work investigated the antidiabetic, anti-hyperlipidemic, and antioxidative effects of Anacardium occidentale methanolic nut extract in Streptozotocin (STZ)-induced diabetic Wistar rats. Methods: Forty (40) Wistar rats weighing 250±30g were randomly divided into five groups of 8 rats each. Group 1 served as the control; Group 2-5 were induced with diabetes with a single dose of 50mg/kg bw of streptozotocin intraperitoneally. After diabetes induction, Group 2 served as the STZ-only group, Groups 3 and 4 were administered 100mg/kg bw and 200mg/kg bw p.o Anacardium occidentale nut extract, respectively, while Group 5 was administered 2mg/kg bw of glimepiride as a reference drug for a period of 4 weeks. Food and water intake were monitored daily, body weight, and blood glucose levels weekly throughout the experiment. On day 29, the animals were sacrificed, and blood samples were collected through cardiac puncture for biochemical studies. Results: Administration of the nut extract significantly (p<0.05) increased the food intake and body weight of diabetic treated rats, fasting blood glucose level and oral glucose tolerance test (OGTT) decreased significantly (p<0.05) in treated rats. On lipid profile, administration of nut extract significantly decreased (p<0.05) triglyceride, low-density lipoprotein, total cholesterol, and very-low-density lipoprotein concentrations while it significantly increases (p<0.05) the high-density lipoprotein. Anacardium occidentale nut extract caused significantly (p<0.05) increases in SOD, GPx, GSH, and CAT levels with a decrease MDA level in diabetic treated rats. Markers of liver and kidney functions were also improved in diabetic treated rats.Conclusion: Treatment with Anacardium occidentale methanolic nut extract has hypoglycemic, hypolipidemic, and hepato-protective effects in diabetic rats. It also alleviates oxidative stress activity and restoration of markers of kidney function, and may be useful as alternative therapy in the management of diabetes and its related complications.

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The Effectiveness of Various Salacca Vinegars as Therapeutic Agent for Management of Hyperglycemia and Dyslipidemia on Diabetic Rats

International Journal of Food Science ◽

10.1155/2017/8742514 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 5

Author(s):

Elok Zubaidah ◽

Widya Dwi Rukmi Putri ◽

Tiara Puspitasari ◽

Umi Kalsum ◽

Dianawati Dianawati

Keyword(s):

Superoxide Dismutase ◽

Blood Glucose ◽

Blood Sugar ◽

Wistar Rats ◽

Therapeutic Agent ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Diabetic Rats ◽

Low Density ◽

Pancreatic Cells

The aim of this study was to explore the potency of salacca vinegar made from various Indonesian salacca fruit extracts as therapeutic agent for hyperglycemia and dyslipidemia for STZ-induced diabetic rats. The rats were grouped into untreated rats, STZ-induced diabetic rats without treatment, and STZ-induced diabetic rats treated with Pondoh salacca vinegar, Swaru salacca vinegar, Gula Pasir salacca vinegar, Madu salacca vinegar, or Madura salacca vinegar. Parameter observed included blood glucose, total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), triglyceride (TG), malondialdehyde (MDA), superoxide dismutase (SOD), and pancreas histopathology of the samples. The results demonstrated that all salacca vinegars were capable of reducing blood sugar (from 25.1 to 62%) and reducing LDL (from 9.5 to 14.8 mg/dL), TG (from 58.3 to 69.5 mg/dL), MDA (from 1.1 to 2.2 mg/dL), and TC (from 56.3 to 70.5 mg/dL) as well as increasing HDL blood sugar of STZ-induced diabetic Wistar rats (from 52.3 to 60 mg/dL). Various salacca vinegars were also capable of regenerating pancreatic cells. Nevertheless, the ability of Swaru salacca vinegar to manage hyperglycemia and dyslipidemia appeared to be superior to other salacca vinegars. Swaru salacca vinegar is a potential therapeutic agent to manage hyperglycemia and dyslipidemia of STZ-induced diabetic rats.

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Impaired-Inactivation of FoxO1 Contributes to Glucose-Mediated Increases in Serum Very Low-Density Lipoprotein

Endocrinology ◽

10.1210/en.2010-0204 ◽

2010 ◽

Vol 151 (8) ◽

pp. 3566-3576 ◽

Cited By ~ 13

Author(s):

Ke Wu ◽

David Cappel ◽

Melissa Martinez ◽

John M. Stafford

Keyword(s):

Blood Glucose ◽

De Novo ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Serum Triglyceride ◽

Very Low Density Lipoprotein ◽

Low Density ◽

Experimental Conditions ◽

Adenoviral Delivery

For patients with diabetes, insulin resistance and hyperglycemia both contribute to increased serum triglyceride in the form of very low-density lipoprotein (VLDL). Our objective was to define the insulin conditions in which hyperglycemia promotes increased serum VLDL in vivo. We performed hyperglycemic-hyperinsulinemic clamp studies and hyperglycemic-hypoinsulinemic clamp studies in rats, with metabolic tracers for glucose flux and de novo fatty acid synthesis. When blood glucose was clamped at hyperglycemia (17 mm) for 2 h under hyperinsulinemic conditions (4 mU/kg · min), serum VLDL levels were not increased compared with baseline. We speculated that hyperinsulinemia minimized glucose-mediated VLDL changes and performed hyperglycemic-hypoinsulinemic clamp studies in which insulin was clamped near fasting levels with somatostatin (17 mm blood glucose, 0.25 mU/kg · min insulin). Under low-insulin conditions, serum VLDL levels were increased 4.7-fold after hyperglycemia, and forkhead box O1 (FoxO1) was not excluded from the nucleus of liver cells. We tested the extent that impaired inactivation of FoxO1 by insulin was sufficient for glucose to promote increased serum VLDL. We found that, when the ability of insulin to inactivate FoxO1 is blocked after adenoviral delivery of constitutively active FoxO1, glucose increased serum VLDL triglyceride when given both by ip glucose tolerance testing (3.5-fold increase) and by a hyperglycemic clamp (4.6-fold). Under both experimental conditions in which insulin signaling to FoxO1 was impaired, we found increased activation of carbohydrate response element binding protein. These data suggest that glucose more potently promotes increased serum VLDL when insulin action is impaired, with either low insulin levels or disrupted downstream signaling to the transcription factor FoxO1.

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Repurposing of Neeri Ayurvedic tablet formulation against obesity: An in-vivo evaluation

Current Drug Therapy ◽

10.2174/1574885516666211207095656 ◽

2021 ◽

Vol 16 ◽

Author(s):

Mohd Abid Malik ◽

Tahira Sultan ◽

Mohit Kumar ◽

Mohd Abid

Keyword(s):

High Density Lipoprotein ◽

Wistar Rats ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

High Density ◽

Heart Weight ◽

Low Density ◽

Standard Drug ◽

Animal House

Background : At present, 63 percent of women and 72 percent of men are obese. Obesity is a condition that causes increased rates of type 2 diabetes mellitus, heart disease, and arthritis, amongst other conditions. It was found that Neeri can control cholesterol and triglycerides levels which are associated with obesity. Therefore, Neeri has been assumed to treat obesity. Based on its efficacy to reduce cholesterol and triglycerides levels, it was assumed that it can be active against obese in-vivo. Methods : In-vivo studies were performed by utilizing albino Wistar rats (100–250g) approved and procured by IAEC resolution no. 2017/837/ac/MPH/12 from the animal house of the Institute of Foreign Trade and Management [IFTM] University, Moradabad, India. The Neeri was administered at different concentrations into five groups of obesity-induced albino Wistar rats, all the groups were treated for 42nd successive days accordingly and the in-vivo tests were performed on the 43rd day. Biochemical serum analysis was measured by ELISA assay. Results : Outcomes were expressed as Mean±SEM. The difference between experimental groups was compared by one-way analysis of variance (ANOVA) followed by Dennett’s test and was considered statistically significant when p<0.05. It was found a significant (p<0.05) decrease in total cholesterol triglycerides, low-density lipoprotein, and very high-density lipoprotein and along with increased high-density lipoprotein. Conclusion : Neeri can reduce body weight, cholesterol, triglycerides, low-density lipoprotein and very-low-density lipoprotein level, liver weight, heart weight, uterine fat, mesenteric fat, and kidneys weight level similar to the standard drug. It was concluded that Neeri Ayurvedic formulation can produce pharmacological action similar to the standard drug, Orlistat. In the future, further studies may proceed and Neeri tablets can be repurposed against obesity.

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Oxidation of Plasma Low-Density Lipoprotein Accelerates Its Accumulation and Degradation in the Arterial Wall In Vivo

Circulation ◽

10.1161/01.cir.94.7.1698 ◽

1996 ◽

Vol 94 (7) ◽

pp. 1698-1704 ◽

Cited By ~ 46

Author(s):

Klaus Juul ◽

Lars B. Nielsen ◽

Klaus Munkholm ◽

Steen Stender ◽

Børge G. Nordestgaard

Keyword(s):

Arterial Wall ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Low Density

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Interaction of 4-hydroxynonenal-modified low-density lipoproteins with the fibroblast apolipoprotein B/E receptor

Biochemical Journal ◽

10.1042/bj2340245 ◽

1986 ◽

Vol 234 (1) ◽

pp. 245-248 ◽

Cited By ~ 31

Author(s):

W Jessup ◽

G Jurgens ◽

J Lang ◽

H Esterbauer ◽

R T Dean

Keyword(s):

Apolipoprotein B ◽

Negative Charge ◽

Low Density Lipoprotein ◽

Ldl Receptor ◽

Density Lipoprotein ◽

Low Density ◽

Lipid Peroxidation Product ◽

Modified Low Density Lipoproteins ◽

Peroxidation Product

The incorporation of the lipid peroxidation product 4-hydroxynonenal into low-density lipoprotein (LDL) increases the negative charge of the particle, and decreases its affinity for the fibroblast LDL receptor. It is suggested that this modification may occur in vivo, and might promote atherogenesis.

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