A Comparative Study of Gene Expression in Menstrual Blood-Derived Stromal Cells between Endometriosis and Healthy Women

Background. Research into the pathogenesis of endometriosis would substantially promote its effective treatment and early diagnosis. Currently, accumulating evidence has shed light on the importance of endometrial stem cells within the menstrual blood which are involved in the establishment and progression of endometriotic lesions in a retrograde manner. Objectives. We aimed to identify the differences in some genes’ expression between menstrual blood-derived mesenchymal stem cells (MenSCs) isolated from endometriosis patients (E-MenSCs) and MenSCs from healthy women (NE-MenSCs). Methods. Menstrual blood samples (2-3 mL) from healthy and endometriosis women in the age range of 22–35 years were collected. Isolated MenSCs by the Ficoll-Paque density-gradient centrifugation method were characterized by flow cytometry. MenSCs were evaluated for key related endometriosis genes by real-time-PCR. Results. E-MenSCs were morphologically different from NE-MenSCs and showed, respectively, higher and lower expression of CD10 and CD9. Furthermore, E-MenSCs had higher expression of Cyclin D1 (a cell cycle-related gene) and MMP-2 and MMP-9 (migration- and invasion-related genes) genes compared with NE-MenSCs. Despite higher cell proliferation in E-MenSCs, the BAX/BCL-2 ratio was significantly lower in E-MenSCs compared to NE-MenSCs. Also, the level of inflammatory genes such as IL1β, IL6, IL8, and NF-κB and stemness genes including SOX2 and SALL4 was increased in E-MenSCs compared with NE-MenSCs. Further, VEGF, as a potent angiogenic factor, showed a significant increase in E-MenSCs rather than NE-MenSCs. However, NE-MenSCs showed increased ER-α and β-catenin when compared with E-MenSCs. Conclusion. Here, we showed that there are gene expression differences between E-MenSCs and NE-MenSCs. These findings propose that MenSCs could play key role in the pathogenesis of endometriosis and further support the menstrual blood retrograde theory of endometriosis formation. This could be of great importance in exploiting promising therapeutic targets and new biomarkers for endometriosis treatment and prognosis.

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Radiation-induced, cell cycle-related gene expression in aging hematopoietic stem cells: enigma of their recovery

Annals of the New York Academy of Sciences ◽

10.1111/nyas.12401 ◽

2014 ◽

Vol 1310 (1) ◽

pp. 69-73 ◽

Cited By ~ 6

Author(s):

Yoko Hirabayashi

Keyword(s):

Gene Expression ◽

Cell Cycle ◽

Stem Cells ◽

Hematopoietic Stem Cells ◽

Related Gene ◽

Hematopoietic Stem ◽

Cell Cycle Related Gene ◽

Radiation Induced

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Gene expression pattern of some classes of cytochrome P-450 and glutathione S-transferase enzymes in differentiated hepatocytes-like cells from menstrual blood stem cells

In Vitro Cellular & Developmental Biology - Animal ◽

10.1007/s11626-014-9857-8 ◽

2015 ◽

Vol 51 (5) ◽

pp. 530-538 ◽

Cited By ~ 3

Author(s):

Aida Esmaeili-Rad ◽

Sayeh Khanjani ◽

Hamidreza Vaziri ◽

Somaieh Kazemnejad

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Expression Pattern ◽

Gene Expression Pattern ◽

Menstrual Blood ◽

Glutathione S Transferase ◽

Menstrual Blood Stem Cells ◽

Blood Stem Cells ◽

Cytochrome P 450

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Conditioned medium from healthy women's endometrial stem cells improve inflammatory and stemness- expression genes in endometriosis women

10.21203/rs.3.rs-249510/v1 ◽

2021 ◽

Author(s):

Seyedeh Saeideh Sahraei ◽

Ali Kowsari ◽

Faezeh Davoodi asl ◽

Mohsen Sheykhhasan ◽

Leila Naserpoor ◽

...

Keyword(s):

Stem Cells ◽

Conditioned Medium ◽

Uterine Cavity ◽

Expression Level ◽

Menstrual Blood ◽

Exterior Region ◽

Healthy Women ◽

Before And After ◽

Benign Gynecological Disease

Abstract Background. Endometriosis is a common, benign gynecological disease which is determined as an overspreading of endometrial tissue in exterior region of the uterine cavity. Evidence suggests that retrograde menstrual blood which contains mesenchymal stem cells with differential gene expression compared to healthy women may play a role in endometriosis creation. We aimed to identify whether the conditioned medium from Menstrual blood-derived stem cells (MenSCs) of healthy women can affect the expression level of inflammatory and stemness genes of MenSCs from endometriosis women. Methods and Results. Endometriosis derived MenSCs (E-MenSCs) were treated with conditioned medium (CM) derived from healthy women’s MenSCs (NE-MenSCs). Some CD markers were analyzed by flow cytometer before and after treatment compared with NE-MenSCs, and the expression level of inflammatory and stemness genes was evaluated by real-time PCR. Results. E-MenSCs show different morphology in vitro culture in comparison with NE-MenSCs, which were changed in the presence of CM, into a morphology more similar to normal cells and showed significant decrease expression of CD10 after CM treatment. In our results, the IL-1, COX-2, and HIF-1\(\alpha\) as an inflamaturay genes and OCT-4, NANOG, and SOX2 as a stemness genes showed significantly different expression level in E-MenSCs after treating with CM. Conclusions. Our study indicates that the expression level of some inflammatory- and stemness-related genes which have differential expression in E-MenSCs compared with NE-MenSCs, could be changed to normal status by using CM derived from NE-MenSCs.

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