Improved Stool DNA Integrity Method for Early Colorectal Cancer Diagnosis

Background. Combination of multiple biomarkers was an effective strategy to improve sensitivity in cancer diagnosis and screening. However, the performance of the combination of methylated SEPT9 and SDC2 for detection of colorectal cancer (CRC) has yet to be reported. Methods. A new qPCR-based assay combining the detection of methylated SEPT9 and SDC2 was used. Methylation statuses of SEPT9 and SDC2 were examined in 19 sets of cancer tissues and paired adjacent tissues and further evaluated with 225 serum samples, including 111 CRC patients and 114 no evidence of disease individuals. Results. SEPT9 and SDC2 methylation levels were higher in 94.7% and 100.0% of cancer tissues than in their paired adjacent tissues. The sensitivities for detecting CRC by SEPT9 methylation alone and SDC2 methylation alone were 73.0% (95% CI: 63.6–80.8%) and 71.2% (95% CI: 61.8–79.2%), respectively, with the same specificity of 95.6% (95% CI: 89.6–98.4%). However, when SEPT9 methylation was combined with SDC2 methylation to detect CRC, the sensitivity was improved to 86.5% (95% CI: 78.4–92.0%) with a specificity of 92.1% (95% CI: 85.1–96.1%). Conclusion. The combination of methylated SEPT9 and SDC2 detection in serum has the potential to be a noninvasive strategy for CRC screening.

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Early colorectal cancer: diagnosis, treatment and survivorship care

Critical Reviews in Oncology/Hematology ◽

10.1016/j.critrevonc.2019.01.023 ◽

2019 ◽

Vol 136 ◽

pp. 20-30 ◽

Cited By ~ 8

Author(s):

Gabriella Buccafusca ◽

Ilaria Proserpio ◽

Antonino Carmelo Tralongo ◽

Sebastiano Rametta Giuliano ◽

Paolo Tralongo

Keyword(s):

Colorectal Cancer ◽

Cancer Diagnosis ◽

Early Colorectal Cancer ◽

Survivorship Care

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Digital Detection of Multiple Minority Mutants in Stool DNA for Noninvasive Colorectal Cancer Diagnosis

Analytical Chemistry ◽

10.1021/ac3008016 ◽

2012 ◽

Vol 84 (13) ◽

pp. 5645-5652 ◽

Cited By ~ 8

Author(s):

Lili Deng ◽

Zongtai Qi ◽

Binjie Zou ◽

Haiping Wu ◽

Huan Huang ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Diagnosis ◽

Digital Detection ◽

Stool Dna ◽

Multiple Minority

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Stool DNA Integrity Method for Colorectal Cancer Detection

Methods in Molecular Biology - Colorectal Cancer ◽

10.1007/978-1-4939-7765-9_12 ◽

2018 ◽

pp. 193-202

Author(s):

Claudia Rengucci ◽

Giulia De Maio ◽

Maura Menghi ◽

Daniele Calistri

Keyword(s):

Colorectal Cancer ◽

Cancer Detection ◽

Dna Integrity ◽

Stool Dna

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Rurality and Other Determinants of Early Colorectal Cancer Diagnosis in Nebraska: A 6-Year Cancer Registry Study, 1998-2003

The Journal of Rural Health ◽

10.1111/j.1748-0361.2009.00244.x ◽

2009 ◽

Vol 25 (4) ◽

pp. 358-365 ◽

Cited By ~ 14

Author(s):

Jayashri Sankaranarayanan ◽

Shinobu Watanabe-Galloway ◽

Junfeng Sun ◽

Fang Qiu ◽

Eugene Boilesen ◽

...

Keyword(s):

Colorectal Cancer ◽

Cancer Registry ◽

Cancer Diagnosis ◽

Early Colorectal Cancer ◽

Registry Study

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KCNQ5 and C9orf50 Methylation in Stool DNA for Early Detection of Colorectal Cancer

Frontiers in Oncology ◽

10.3389/fonc.2020.621295 ◽

2021 ◽

Vol 10 ◽

Author(s):

Yaping Cao ◽

Guodong Zhao ◽

Mufa Yuan ◽

Xiaoyu Liu ◽

Yong Ma ◽

...

Keyword(s):

Colorectal Cancer ◽

Clinical Performance ◽

Advanced Adenoma ◽

Early Colorectal Cancer ◽

Crc Screening ◽

Alternative Approach ◽

Significant Difference ◽

Stool Dna ◽

Aberrant Dna Methylation ◽

Screening And Prevention

BackgroundAberrant DNA methylation has emerged as a class of promising biomarkers for early colorectal cancer (CRC) detection, but the performance of methylated C9orf50 and methylated KCNQ5 in stool DNA has never been evaluated.MethodsMethylation specific quantitative PCR (qPCR) assays for methylated C9orf50 and methylated KCNQ5 were developed. The methylation levels of C9orf50 and KCNQ5 in 198 CRC patients, 20 advanced adenoma (AA) patients, 101 small polyp (SP) patients, and 141 no evidence of disease (NED) subjects were analyzed.ResultsThe methylation levels of both KCNQ5 and C9orf50 genes were significantly higher in CRC and AA groups than those in SP and NED groups, but showed no significant difference among different stages of CRC. The sensitivities of methylated KCNQ5 and methylated C9orf50 for CRC detection were 77.3% (95% CI: 70.7–82.8%) and 85.9% (95% CI: 80.0–90.2%) with specificities of 91.5% (95% CI: 85.3–95.3%) and 95.0% (95% CI: 89.7–97.8%), respectively. When C9orf50 and methylated KCNQ5 were combined, the clinical performance for CRC detection was similar to that of methylated C9orf50 alone.ConclusionsStool DNA based methylated C9orf50 test has the potential to become an alternative approach for CRC screening and prevention.

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Endoskopinių tyrimų reikšmė storosios žarnos vėžio ankstyvajai diagnostikai

Lietuvos chirurgija ◽

10.15388/lietchirur.2003.3.2404 ◽

2003 ◽

Vol 1 (3) ◽

pp. 0-0

Author(s):

Pavel Elsakov

Keyword(s):

Colorectal Cancer ◽

Colon Cancer ◽

Relative Risk ◽

Cancer Diagnosis ◽

Early Cancer ◽

Early Colorectal Cancer ◽

Early Cancer Diagnosis ◽

Number Of Patients ◽

Early Colon Cancer ◽

Oncology Center

Pavel ElsakovVilniaus universiteto Onkologijos institutas,Santariškių g. 1, LT-2021 VilniusEl paštas: [email protected] Įvadas / tikslas Ankstyvojo storosios žarnos vėžio diagnostika – viena opiausių problemų tiek Lietuvoje, tiek Vakarų šalyse. Šio darbo tikslas – įvertinti endoskopinių tyrimų reikšmę storosios žarnos vėžio ankstyvajai diagnostikai. Metodai Lietuvos onkologijos centre 1998–2000 metais, palyginti su 1995–1997 metais, kolonoskopuojamų simptominių ligonių skaičius padidėjo du kartus. Statistiniu metodu (santykine rizika RR) apskaičiavome kiekvieno storosios žarnos segmento C18.0; C18.2–7; C19; C20 (aklosios, gaubtinės žarnos, rektosigminės jungties, tiesiosios žarnos) ankstyvojo vėžio diagnostikos pokyčius. Rezultatai Lietuvos onkologijos centre, 1998–2000 metais, palyginti su 1995–1997 metais, dukart padidinus kolonoskopuojamų simptominių ligonių skaičių, ankstyvojo storosios žarnos vėžio diagnostika pagerėjo: rektosigminės jungties vėžio – 1,22 karto (RR = 1,22), aklosios žarnos – 1,47 karto (RR = 1,47). Išvados Kolonoskopuojamų simptominių pacientų skaičių padidinus dukart, I–II stadijos aklosios žarnos vėžio diagnostika pagerėjo 1,47 karto, rektosigminės jungties – 1,22 karto. Prasminiai žodžiai: kolonoskopija, rektoskopija, ankstyvasis vėžys, santykinė rizika. The value of endoscopical examinations for improving the early colorectal cancer diagnosis Pavel Elsakov Background The early colorectal cancer diagnosis in Lithuania and West countries remain one of the general and important tasks. Methods At Lithuanian Oncology Center (LOC) in1998–2000 versus 1995–1997 the number of symptomatic patients examined by colonoscopy increased twice. For analysis of early colorectal cancer diagnosis we used the relative risk calculation method. Results In 1998–2000 versus 1995–1998, at LOC the early colon cancer diagnosis improved: cecum 1.47 times (RR = 1.47), rectosigmoid 1.22 (RR = 1.22). The early cancer diagnosis of rectum did not change, because did not change the number of patients examined by rectoscopy. Conclusions The twice as high number of symptomatic patients examined by colonoscopy improved diagnosis of early colon cancer, especially of its cecum (1.47 times) and colon rectosigmoid (1.22 times) forms. Keywords: colonoscopy, rectoscopy, early cancer, relative risk.

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