GEISHA: an in situ hybridization gene expression resource for the chicken embryo

2007 ◽  
Vol 117 (1-4) ◽  
pp. 30-35 ◽  
Author(s):  
D.K. Darnell ◽  
S. Kaur ◽  
S. Stanislaw ◽  
S. Davey ◽  
J.H. Konieczka ◽  
...  
2007 ◽  
Vol 86 (7) ◽  
pp. 1472-1477 ◽  
Author(s):  
P.B. Antin ◽  
S. Kaur ◽  
S. Stanislaw ◽  
S. Davey ◽  
J.H. Konieczka ◽  
...  

2021 ◽  
Vol 4 (1) ◽  
pp. 20
Author(s):  
Mujeeb Shittu ◽  
Tessa Steenwinkel ◽  
William Dion ◽  
Nathan Ostlund ◽  
Komal Raja ◽  
...  

RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model Drosophila species.


2004 ◽  
Vol 229 (3) ◽  
pp. 651-657 ◽  
Author(s):  
Nathaniel Denkers ◽  
Pilar García-Villalba ◽  
Christopher K. Rodesch ◽  
Kandice R. Nielson ◽  
Teri Jo Mauch

1991 ◽  
Vol 88 (18) ◽  
pp. 8154-8158 ◽  
Author(s):  
A. M. Henney ◽  
P. R. Wakeley ◽  
M. J. Davies ◽  
K. Foster ◽  
R. Hembry ◽  
...  

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