Studies on the Toxicity and Binding Kinetics of Abrin in Normal and Epstein Barr Virus-Transformed Lymphocyte Culture-I: Experimental Results &ndash; 1

Abstract The Epstein-Barr virus (EBV) is known to cause posttransplant lymphoproliferative disease (PTLD) in immunosuppressed transplant patients. The results of this pilot study showed that all EBV- patients pretransplant experienced primary EBV infection within the first 3 months after transplant surgery. Virtually all of these patients had a higher burden of EBV-infected cells in their peripheral blood (PB) after infection by EBV than did the EBV+ pretransplant group when tested at the same intervals posttransplant. Salivary EBV titers also increased in most patients, but the difference between the two groups was statistically significant only at 12 months, whereupon EBV+ patients showed higher titers compared with EBV- (alpha < 0.053). Also, polymerase chain reaction amplification followed by Southern blotting was performed to detect EBV sequences in PB mononuclear cells. This technique allowed confirmation of the blood culture results and constituted a faster alternative compared with the culture assay. The highest increase in the number of EBV-infected lymphocytes at 3 months posttransplant obtained from PB was seen in a patient who developed fatal PTLD and in another with protracted infectious mononucleosis. Thus, the number of EBV-infected cells in PB was found to correlate positively with risk of development of PTLD at 3 months posttransplant in our group of pediatric transplant patients. This study showed that quantitative lymphocyte culture of PB was an accurate index of immunosuppression and a reliable method for assessing the risk of PTLD development.

Download Full-text

Lymphoblastoid transformation and kinetics of appearance of viral nuclear antigen (EBNA) in cord-blood lymphocytes infected by epstein-barr virus (EBV)

International Journal of Cancer ◽

10.1002/ijc.2910150303 ◽

1975 ◽

Vol 15 (3) ◽

pp. 377-384 ◽

Cited By ~ 33

Author(s):

J. Yata ◽

C. Desgranges ◽

T. Nakagawa ◽

M. C. Favre ◽

G. De-Thé

Keyword(s):

Cord Blood ◽

Epstein Barr Virus ◽

Nuclear Antigen ◽

Blood Lymphocytes ◽

Barr Virus ◽

Epstein Barr ◽

Kinetics Of ◽

Cord Blood Lymphocytes

Download Full-text

Infection of human B lymphocytes with high multiplicities of Epstein-Barr virus: Kinetics of EBNA expression, cellular DNA synthesis, and mitosis

Virology ◽

10.1016/0042-6822(81)90504-3 ◽

1981 ◽

Vol 109 (2) ◽

pp. 336-343 ◽

Cited By ~ 27

Author(s):

James Robinson ◽

Douglas Smith

Keyword(s):

Dna Synthesis ◽

B Lymphocytes ◽

Epstein Barr Virus ◽

Barr Virus ◽

Epstein Barr ◽

Cellular Dna ◽

Kinetics Of

Download Full-text

Reactivation of lymphocryptovirus (Epstein–Barr virus chimpanzee) and dominance in chimpanzees

Journal of General Virology ◽

10.1099/vir.0.022376-0 ◽

2010 ◽

Vol 91 (8) ◽

pp. 2049-2053 ◽

Cited By ~ 8

Author(s):

Rain Yamamoto ◽

Migaku Teramoto ◽

Ikuo Hayasaka ◽

Koki Ikeda ◽

Toshikazu Hasegawa ◽

...

Keyword(s):

Viral Load ◽

Epstein Barr Virus ◽

Group Formation ◽

Dominance Rank ◽

Male Dominance ◽

Barr Virus ◽

Immunological Stress ◽

Epstein Barr ◽

Set Up ◽

Kinetics Of

Nine male chimpanzees originally reared in solitary cages were set up to form a group. Plasma viral load of the lymphocryptovirus (LCV) of chimpanzee [Epstein–Barr virus chimpanzee (EBVcmp)] was measured by real-time PCR. In the group formation (Form) period, the first-ranking male showed an imminent increase in plasma EBVcmp load compared with 1 week before (pre-Form) and 3 months after (post-Form) group formation. Other upper-ranking males such as the second-, third- and fourth-male also showed the highest level of viral load in the Form period. The kinetics of EBVcmp load in the Form period were statistically different from other periods (against pre-Form, t=−4.878, P<0.001; against post-Form, t=6.434, P<0.001). The effect of the male dominance rank did not differ between the pre-Form and post-Form periods (t=−1.557, P=0.12). Reactivation of LCV (EBV) as an immunological stress marker for humans might also be applied to chimpanzees.

Download Full-text