Nucleic Acid Amplification of Mycobacterium tuberculosis Complex DNA from Archival Fine Needle Aspiration Smear Scrapings vs. Fresh Fine Needle Aspirates of Tuberculous Lymphadenitis

Background: The extrapulmonary tuberculosis (EPTB) is challenging to diagnose due to its pauci-bacillary nature. According to recent research, WHO recommends cartridge based nucleic acid amplification test (CBNAAT) to be used as initial diagnostic test in suspected cases of extrapulmonary tuberculosis. Aim of the present study is to assess the role of CBNAAT in comparison with cytomorphological features upon fine needle aspiration cytology (FNAC) and Ziehl-Neelson (ZN) stain in clinically suspected cases of EPTB.Methods: Present pilot study is descriptive cross-sectional study of 439 cases of clinically suspected EPTB over a period of 12 months (January 2019 to December 2019). After procedure of fine needle aspirates, smears were stained with routine H&E, papanicolaou stain and ZN stain. In the same setting, aspirate was also sent for CBNAAT. Results were obtained after detailed study.Results: Out of 439 cases, presumptive tuberculosis was diagnosed in 192 cases showing either epithelioid cell granulomas or caseous necrosis or both upon morphology, while overall 94 cases were positive on ZN stain and 146 cases were CBNAAT positive with the sensitivity of 84.04% and specificity of 80.57%.Conclusions: FNAC is the cheapest and simplest method to diagnose extrapulmonary tuberculosis, however those smears where tuberculosis cannot be diagnosed on FNAC like suppurative lesions, reactive lymphadenitis and low cellularity, CBNAAT plays a key role for the correct diagnosis thereby significantly reducing the morbidity and mortality.

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Evaluation of Three Nucleic Acid Amplification Methods for Direct Detection of Mycobacterium tuberculosis Complex in Respiratory Specimens

Journal of Clinical Microbiology ◽

10.1128/jcm.37.6.1932-1934.1999 ◽

1999 ◽

Vol 37 (6) ◽

pp. 1932-1934 ◽

Cited By ~ 25

Author(s):

S. X. Wang ◽

L. Tay

Keyword(s):

Mycobacterium Tuberculosis ◽

Nucleic Acid ◽

Mycobacterium Tuberculosis Complex ◽

Direct Detection ◽

Nucleic Acid Amplification ◽

Smear Microscopy ◽

Direct Test ◽

Tuberculosis Complex ◽

Chain Reaction ◽

Respiratory Specimens

Two hundred thirty respiratory specimens from 230 patients were analyzed by using COBAS AMPLICOR PCR, Amplified Mycobacterium tuberculosis Direct Test, and ligase chain reaction methods. Results were compared with those of smear microscopy and radiometric culture (Bactec) methods. No significant differences were observed among the results of the three methods, which are acceptable for direct detection of M. tuberculosis complex in respiratory specimens.

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The rational use of nucleic acid amplification testing for the Mycobacterium tuberculosis complex

Microbiology Australia ◽

10.1071/ma04428 ◽

2004 ◽

Vol 25 (4) ◽

pp. 28

Author(s):

Richard Lumb

Keyword(s):

Mycobacterium Tuberculosis ◽

Nucleic Acid ◽

Clinical Significance ◽

Mycobacterium Bovis ◽

Mycobacterium Tuberculosis Complex ◽

Nucleic Acid Amplification ◽

Tuberculosis Complex ◽

Rational Use ◽

Genus Mycobacterium ◽

Mycobacterium Microti

On the basis of clinical significance, Mycobacterium tuberculosis is the most important member of the genus Mycobacterium. It is closely related genetically to Mycobacterium bovis, M. africanum, Mycobacterium microti, Mycobacterium bovis BCG (the bacillus of Calmette-Guerin) and the recently described Mycobacterium tuberculosis subspecies Canetti. Together they are termed the Mycobacterium tuberculosis complex (MTBC).

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LYMPHOID INFILTRATION IN TOXIC GOITRES STUDIED WITH FINE NEEDLE ASPIRATION BIOPSY

Acta Endocrinologica ◽

10.1530/acta.0.0710480 ◽

1972 ◽

Vol 71 (3) ◽

pp. 480-490 ◽

Cited By ~ 3

Author(s):

Göran Nilsson

Keyword(s):

Fine Needle Aspiration ◽

Fine Needle Aspiration Biopsy ◽

Needle Aspiration ◽

Lymphoid Cells ◽

Aspiration Biopsy ◽

Fine Needle ◽

Fine Needle Aspirates ◽

Needle Aspiration Biopsy ◽

Lymphoid Infiltration ◽

Long Acting

ABSTRACT Cytodiagnostic fine needle aspiration biopsy specimens from toxic goitres were studied for signs of lymphoid infiltration. Comparison with histological sections of specimens obtained by surgery showed that an excess of lymphoid cells in the aspirate smears corresponded to a large number of lymphoid foci in these sections. Excess of lymphoid cells in the fine needle aspirates was also positively correlated with the occurrence of circulating thyroid antibodies against thyroglobulin and/or cytoplasmic antigen, but not with the presence of the long-acting thyroid stimulating factor, LATS. It also varied with age in that it was most common in the youngest patients and in patients between 40–55 years, while lymphoid infiltration was seldom seen in patients over 55 years. A finding of practical clinical interest was that in toxic goitres with cytological signs of lymphoid infiltration hyperthyroidism had less tendency to recur after treatment with thiocarbamide drugs than in those without such signs.

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