A Comparative Study of Bronchoscopic Microsample Probe versus Bronchoalveolar Lavage in Patients with Burns-Related Inhalational Injury, Acute Lung Injury and Chronic Stable Lung Disease

Bronchoconstriction responses in the airway are caused by multiple insults and are the hallmark symptom in asthma. In an acute lung injury model in mice, IgG immune complex deposition elicited severe airway hyperreactivity that peaked by 1 h, was maintained at 4 h, and was resolved by 24 h. The depletion of complement with cobra venom factor (CVF) markedly reduced the hyperreactive airway responses, suggesting that complement played an important role in the response. Blockade of C5a with specific antisera also significantly reduced airway hyperreactivity in this acute lung model. Complement depletion by CVF treatment significantly reduced tumor necrosis factor and histamine levels in bronchoalveolar lavage fluids, correlating with reductions in airway hyperreactivity. To further examine the role of specific complement requirement, we initiated the immune complex response in C5-sufficient and C5-deficient congenic animals. The airway hyperreactivity response was partially reduced in the C5-deficient mice. Complement depletion with CVF attenuated airway hyperreactivity in the C5-sufficient mice but had a lesser effect on the airway hyperreactive response and histamine release in bronchoalveolar lavage fluids in C5-deficient mice. These data indicate that acute lung injury in mice after deposition of IgG immune complexes induced airway hyperreactivity that is C5 and C5a dependent.

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Optimization Of An LC-MS Approach For The Identification Of Acute Lung Injury (ALI) Metabolites In Bronchoalveolar Lavage Fluid (BALF)

10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a2125 ◽

2012 ◽

Author(s):

Kathleen A. Stringer ◽

Charles Evans ◽

Chunhai Ruan ◽

Alla Karnovsky ◽

Theodore Standiford

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid

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Modulation of endoperoxide product levels and cyclophosphamide-induced injury by glutathione repletion

Journal of Applied Physiology ◽

10.1152/jappl.1989.67.6.2316 ◽

1989 ◽

Vol 67 (6) ◽

pp. 2316-2322 ◽

Cited By ~ 12

Author(s):

J. A. Cooper ◽

W. W. Merrill

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Bronchoalveolar Lavage ◽

Arachidonic Acid Metabolism ◽

Glutathione Depletion ◽

Enzymatic Reactions ◽

Variable Degree ◽

Cyclophosphamide Treatment ◽

Dose Dependent

Glutathione is a tripeptide important in a number of diverse cellular functions including enzymatic reactions involved in prostaglandin endoperoxide metabolism. We have previously reported that cyclophosphamide administration to rats results in acute lung injury manifested by increased bronchoalveolar lavage albumin concentrations. In the current study we examine whether cyclophosphamide treatment affects pulmonary glutathione stores or bronchoalveolar endoperoxide metabolic product levels and whether these effects may be related to acute lung injury caused by the drug. We show that cyclophosphamide treatment causes a dose-dependent reduction in pulmonary glutathione stores 4 h after drug administration. In addition, acute lung injury as the result of cyclophosphamide can be abrogated by coadministration of oxothiazolidine carboxylate, an intracellular cysteine delivery system that also reverses pulmonary glutathione depletion induced by cyclophosphamide in our study. Finally, cyclophosphamide treatment reduces prostaglandin E2 concentrations in bronchoalveolar lavage and alveolar macrophage culture supernatant in a dose-dependent fashion and increases bronchoalveolar thromboxane concentrations in low dose-treated animals. These effects are reversed to a variable degree by coadministration of oxothiazolidine carboxylate. Our study suggests in vivo pulmonary arachidonic acid metabolism and cyclophosphamide-induced acute lung injury are modulated by cellular glutathione stores. These findings may have important implications for the treatment of acute lung injury.

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Metabolomic profile perturbations of serum, lung, bronchoalveolar lavage fluid, spleen and feces in LPS-induced acute lung injury rats based on HPLC-ESI-QTOF-MS

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-019-02357-1 ◽

2020 ◽

Vol 412 (5) ◽

pp. 1215-1234 ◽

Cited By ~ 2

Author(s):

Tianyang Wang ◽

Song Lin ◽

Ran Liu ◽

Hua Li ◽

Zihan Liu ◽

...

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Metabolomic Profile

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A rise of MMP-2 and MMP-9 in bronchoalveolar lavage fluid is associated with acute lung injury after cardiopulmonary bypass in a swine model

Perfusion ◽

10.1191/0267659103pf662oa ◽

2003 ◽

Vol 18 (2) ◽

pp. 107-113 ◽

Cited By ~ 12

Author(s):

Wolfgang Eichler ◽

J F Matthias Bechtel ◽

Jan Schumacher ◽

Johanna A Wermelt ◽

Karl-Friedrich Klotz ◽

...

Keyword(s):

Acute Lung Injury ◽

Cardiopulmonary Bypass ◽

Lung Injury ◽

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Lavage Fluid ◽

Swine Model ◽

Tissue Samples ◽

Pulmonary Capillary ◽

A Prospective Study

Postoperative acute lung injury (ALI) contributes to the morbidity and mortality following cardiopulmonary bypass (CPB). To determine whether the presence of matrix metalloproteinases (MMPs) is associated with ALI after CPB, MMP-2 and MMP-9 activities in bronchoalveolar lavage fluid (BALF) were compared with parameters indicating impaired gas exchange. In a prospective study, 17 minipigs were subjected to CPB for 60 min. Before and at five and 180 min after CPB, MMP-2 and MMP-9 were assayed in BALF and the arterial-alveolar gradient of oxygen tension (AaDO2), the pulmonary capillary wedge pressure (PCWP) and the water content of lung tissue samples (Wt) were evaluated and compared with baseline values. MMP-2 and MMP-9 increased significantly 5 minutes (2.1- and 6.2-fold, respectively) and 180 minutes (3.4- and 14.3-fold, respectively) post-CPB. AaDO2 and Wt, but not PCWP, increased significantly 180 minutes after CPB and only AaDO2, but not PCWP or Wt, was significantly correlated with MMP-2 (r/0.66, p/0.006) and MMP-9 (r/0.62, p/0.01). In conclusion, high levels of MMP-2 and MMP-9 in the pulmonary compartment are associated with ALI after CPB.

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Comparative Study On Murine Model Of Acute Lung Injury By Non-Exposed And Exposed Intratracheal Instillations

10.1164/ajrccm-conference.2012.185.1_meetingabstracts.a2110 ◽

2012 ◽

Author(s):

Bo Li ◽

Chunling Dong ◽

Ling Liu ◽

Zhongsen Ma ◽

Xiangdong Wang ◽

...

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Comparative Study ◽

Murine Model

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A Non-Specific Bronchoalveolar Lavage Finding May Help Treat an Increasingly Common Etiology of Acute Lung Injury

10.1164/ajrccm-conference.2020.201.1_meetingabstracts.a7014 ◽

2020 ◽

Author(s):

K. Boyd ◽

O. Tamimi ◽

M. Khan ◽

C. Cortes ◽

J. Murthy

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Bronchoalveolar Lavage ◽

Common Etiology

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Dynamic mechanical consequences of deep inflation in mice depend on type and degree of lung injury

Journal of Applied Physiology ◽

10.1152/japplphysiol.00270.2003 ◽

2004 ◽

Vol 96 (1) ◽

pp. 293-300 ◽

Cited By ~ 50

Author(s):

Gilman Allen ◽

Jason H. T. Bates

Keyword(s):

Acute Lung Injury ◽

Lung Injury ◽

Bronchoalveolar Lavage ◽

Bronchoalveolar Lavage Fluid ◽

Fluid Pressure ◽

Lavage Fluid ◽

Forced Oscillations ◽

Positive End Expiratory Pressure ◽

Reduced Pressure ◽

Volume Curve

In a previous study (Allen G, Lundblad LK, Parsons P, and Bates JH. J Appl Physiol 93: 1709-1715 , 2002), our laboratory used deep inflations (DI) in mice to show that recruitment of closed lung units can be a very transient phenomenon in lung injury. The purpose of this study was to investigate how this transience of lung recruitment depends on the nature and degree of acute lung injury. Mice were administered 50 μl of either saline ( n = 8), 0.01 M ( n = 9) or 0.025 M ( n = 8) hydrochloric acid, or 50 μg ( n = 10) or 150 μg ( n = 6) of LPS and were mechanically ventilated 24-48 h later. At various levels of positive end-expiratory pressure, two DIs were delivered, and forced oscillations were used to obtain a measure of lung stiffness ( H) periodically over 7 min. After LPS exposure, pressure-volume curve hysteresis and recovery in H after DI were no different from saline-exposed controls despite 500 times more neutrophils in bronchoalveolar lavage fluid. Pressure-volume hysteresis and recovery in H were increased in acid-exposed mice ( P < 0.001) and were correlated with bronchoalveolar lavage fluid protein content ( R = 0.81). Positive end-expiratory pressure reduced recovery in H in all groups ( P < 0.01) but reduced pressure-volume hysteresis in the acid-injured groups only ( P < 0.001). We conclude that the effects of DIs in acute lung injury depend on the degree of lung injury but only to the extent that this injury reflects a disruption of the air-liquid interface.

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