Generalized Reaction of Bone Marrow in Neoplastic Disorders

Abstract Eosinophil granules are intensely autofluorescent when excited by green light. To determine if eosinophils degranulate in the bone marrows of patients with a variety of diseases, we used green light epifluorescence microscopy to examine deparaffinized and dezenkerized sections of 49 bone marrow core biopsies. In 14 of the biopsies, there was striking extracellular deposition of intensely autofluorescent eosinophil granules in addition to numerous intact eosinophils. Among the 14 specimens with extracellular autofluorescence were seven cases of leukemia, four cases of non-Hodgkin's lymphoma, two cases of myelofibrosis, and one case of pancytopenia with eosinophilia. In the remaining 35 specimens, only intact eosinophils were identifiable. There was no extracellular autofluorescence in three normal marrows, four marrows from AIDS patients, or three biopsies from patients with idiopathic thrombocytopenic purpura (ITP). We conclude that green light epifluorescence microscopy identifies extracellular deposits of eosinophil granules in bone marrow biopsies of some neoplastic disorders and in diseases associated with reticulin fibrosis.

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Deposition of autofluorescent eosinophil granules in pathologic bone marrow biopsies

Blood ◽

10.1182/blood.v70.2.597.bloodjournal702597 ◽

1987 ◽

Vol 70 (2) ◽

pp. 597-599

Author(s):

MK Samoszuk ◽

FP Espinoza

Keyword(s):

Bone Marrow ◽

Idiopathic Thrombocytopenic Purpura ◽

Green Light ◽

Hodgkin's Lymphoma ◽

Epifluorescence Microscopy ◽

Aids Patients ◽

Thrombocytopenic Purpura ◽

Bone Marrow Biopsies ◽

Non Hodgkin's Lymphoma ◽

Neoplastic Disorders

Eosinophil granules are intensely autofluorescent when excited by green light. To determine if eosinophils degranulate in the bone marrows of patients with a variety of diseases, we used green light epifluorescence microscopy to examine deparaffinized and dezenkerized sections of 49 bone marrow core biopsies. In 14 of the biopsies, there was striking extracellular deposition of intensely autofluorescent eosinophil granules in addition to numerous intact eosinophils. Among the 14 specimens with extracellular autofluorescence were seven cases of leukemia, four cases of non-Hodgkin's lymphoma, two cases of myelofibrosis, and one case of pancytopenia with eosinophilia. In the remaining 35 specimens, only intact eosinophils were identifiable. There was no extracellular autofluorescence in three normal marrows, four marrows from AIDS patients, or three biopsies from patients with idiopathic thrombocytopenic purpura (ITP). We conclude that green light epifluorescence microscopy identifies extracellular deposits of eosinophil granules in bone marrow biopsies of some neoplastic disorders and in diseases associated with reticulin fibrosis.

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Autologous Bone Marrow Infusion as an Adjunct in Therapy of Malignant Disease

Blood ◽

10.1182/blood.v14.5.503.503 ◽

1959 ◽

Vol 14 (5) ◽

pp. 503-521 ◽

Cited By ~ 75

Author(s):

WILLIAM MCFARLAND ◽

NORMA B. GRANVILLE ◽

WILLIAM DAMESHEK

Keyword(s):

Bone Marrow ◽

Intravenous Infusion ◽

Malignant Disease ◽

Tumor Tissue ◽

Nitrogen Mustard ◽

Autologous Bone Marrow ◽

Autologous Bone ◽

Neoplastic Disorders ◽

Marrow Infusion ◽

Bone Marrow Infusion

Abstract 1. Three patients with lymphosarcomatosis and two with disseminated Hodgkin’s disease were treated with massive doses (super-dosage) of nitrogen mustard (over 1.0 mg./Kg.) given intravenously, followed by the intravenous infusion of autologous bone marrow. 2. Two of the five patients succumbed to infection. The remaining three patients recovered within one month and experienced brief remissions. 3. It is suggested that this form of therapy merits further trial. Thus, it may be considered for use earlier in the course of the disease when there is a smaller total amount of tumor tissue, and thus a greater possibility for its complete eradication. Its use in other neoplastic disorders, as following operative procedures disclosing malignancy, is suggested.

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Bone Marrow Clot Section a Useful Tool for Diagnosis of Haematological Diseases- A Case Series

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2021/49056:15028 ◽

2021 ◽

Author(s):

Parul Garg ◽

Harjot Kaur ◽

Ishwer Tayal ◽

Aradhana Singh Hada

Keyword(s):

Bone Marrow ◽

Diagnostic Tool ◽

Core Biopsy ◽

Case Series ◽

Bone Marrow Aspiration ◽

Bone Marrow Examination ◽

Biopsy Material ◽

Haematological Disorders ◽

Neoplastic Disorders

Bone marrow examination is required for diagnosis of various haematological disorders. It includes both neoplastic and non-neoplastic disorders. Usually, bone marrow examination includes Bone Marrow Aspiration (BMA), bone marrow biopsy and bone marrow imprints. Bone marrow clot sections can also be an adjuvant of bone marrow examination. The bone marrow clot sections are prepared from the left-over blood after aspirate smears have been prepared. An adequate bone marrow clot section can be valuable for diagnosis of various diseases, especially in cases in which the aspirate and core biopsy material are inadequate or non contributory or it can be an adjuvant procedure. Little has been published about usefulness of bone marrow clot section. Studies are going on to evaluate the role of bone marrow clot section as an adjuvant or a diagnostic tool. This study includes a series of three cases in which bone marrow clot section was diagnostic.

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Glycogen in guinea pig neutrophils: Ultrastructural study of neutrophils at the intradermal site of BCG injection

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100077207 ◽

1983 ◽

Vol 41 ◽

pp. 726-727

Author(s):

Corazon D. Bucana

Keyword(s):

Bone Marrow ◽

Guinea Pig ◽

Electron Density ◽

Peritoneal Cavity ◽

Ultrastructural Study ◽

Guinea Pigs ◽

Random Distribution ◽

Glycogen Content ◽

Polymorphonuclear Neutrophils ◽

Ultrastructural Studies

In the circulating blood of man and guinea pigs, glycogen occurs primarily in polymorphonuclear neutrophils and platelets. The amount of glycogen in neutrophils increases with time after the cells leave the bone marrow, and the distribution of glycogen in neutrophils changes from an apparently random distribution to large clumps when these cells move out of the circulation to the site of inflammation in the peritoneal cavity. The objective of this study was to further investigate changes in glycogen content and distribution in neutrophils. I chose an intradermal site because it allows study of neutrophils at various stages of extravasation.Initially, osmium ferrocyanide and osmium ferricyanide were used to fix glycogen in the neutrophils for ultrastructural studies. My findings confirmed previous reports that showed that glycogen is well preserved by both these fixatives and that osmium ferricyanide protects glycogen from solubilization by uranyl acetate.I found that osmium ferrocyanide similarly protected glycogen. My studies showed, however, that the electron density of mitochondria and other cytoplasmic organelles was lower in samples fixed with osmium ferrocyanide than in samples fixed with osmium ferricyanide.

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Mammalian Bone Marrow Acetylcholinesterase

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053024 ◽

1982 ◽

Vol 40 ◽

pp. 44-45

Author(s):

Ezzatollah Keyhani

Keyword(s):

Central Nervous System ◽

Bone Marrow ◽

Nervous System ◽

Common Property ◽

Extracellular Medium ◽

The Central Nervous System ◽

The Common ◽

Mammalian Bone

Acetylcholinesterase (EC 3.1.1.7) (ACHE) has been localized at cholinergic junctions both in the central nervous system and at the periphery and it functions in neurotransmission. ACHE was also found in other tissues without involvement in neurotransmission, but exhibiting the common property of transporting water and ions. This communication describes intracellular ACHE in mammalian bone marrow and its secretion into the extracellular medium.

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The structure of pre-mRNA and mRNA from erythroid cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100082030 ◽

1978 ◽

Vol 36 (2) ◽

pp. 234-235

Author(s):

A.-M. Ladhoff ◽

B.J. Thiele ◽

Ch. Coutelle ◽

S. Rosenthal

Keyword(s):

Bone Marrow ◽

Structural Transformation ◽

Electron Micrographs ◽

Ammonium Acetate ◽

Bone Marrow Cells ◽

Erythroid Cells ◽

Globin Mrna ◽

Ordered Structures ◽

Rabbit Bone ◽

Rabbit Reticulocytes

The suggested precursor-product relationship between the nuclear pre-mRNA and the cytoplasmic mRNA has created increased interest also in the structure of these RNA species. Previously we have been published electron micrographs of individual pre-mRNA molecules from erythroid cells. An intersting observation was the appearance of a contour, probably corresponding to higher ordered structures, on one end of 10 % of the pre-mRNA molecules from erythroid rabbit bone marrow cells (Fig. 1A). A virtual similar contour was observed in molecules of 9S globin mRNA from rabbit reticulocytes (Fig. 1B). A structural transformation in a linear contour occurs if the RNA is heated for 10 min to 90°C in the presence of 80 % formamide. This structural transformation is reversible when the denatured RNA is precipitated and redissolved in 0.2 M ammonium acetate.

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Sinusoidal Cells in Bone Marrow Take Up BSA-Au Conjugates in the Presence of an Artificial Blood Substitute

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100120199 ◽

1985 ◽

Vol 43 ◽

pp. 700-701

Author(s):

J.S. Geoffroy ◽

R.P. Becker

Keyword(s):

Bone Marrow ◽

Los Angeles ◽

Iliac Artery ◽

Blood Substitute ◽

Sprague Dawley ◽

Coated Pits ◽

Sinusoidal Cells ◽

Artificial Blood ◽

Left Common Iliac Artery

The pattern of BSA-Au uptake in vivo by endothelial cells of the venous sinuses (sinusoidal cells) of rat bone marrow has been described previously. BSA-Au conjugates are taken up exclusively in coated pits and vesicles, enter and pass through an “endosomal” compartment comprised of smooth-membraned tubules and vacuoles and cup-like bodies, and subsequently reside in multivesicular and dense bodies. The process is very rapid, with BSA-Au reaching secondary lysosmes one minute after presentation. (Figure 1)In further investigations of this process an isolated limb perfusion method using an artificial blood substitute, Oxypherol-ET (O-ET; Alpha Therapeutics, Los Angeles, CA) was developed. Under nembutal anesthesia, male Sprague-Dawley rats were laparotomized. The left common iliac artery and vein were ligated and the right iliac artery was cannulated via the aorta with a small vein catheter. Pump tubing, preprimed with oxygenated 0-ET at 37°C, was connected to the cannula.

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