Avian Collagen Is Useful for the Construction of Skin Equivalents

2017 ◽  
Vol 204 (5-6) ◽  
pp. 261-269 ◽  
Author(s):  
Hailan Li ◽  
Hye-Young Yun ◽  
Kwang Jin Baek ◽  
Nyoun Soo Kwon ◽  
Hye-Ryung Choi ◽  
...  
Keyword(s):  
Proliferating Cell Nuclear Antigen ◽  
Immunohistochemical Staining ◽  
Nuclear Antigen ◽  
Integrin Β1 ◽  
Skin Equivalents ◽  
Test Models ◽  
Hematoxylin And Eosin ◽  
Alternative Test ◽  
Proliferating Cell ◽  
Do So

As a result of restrictions on animal experimentation, improved skin equivalents (SEs) are needed as alternative test models. This work investigated the effects of avian collagen on the construction of SEs, and to the best of our knowledge is the first study to do so. Hematoxylin and eosin and immunohistochemical staining were used to analyze the SEs. In models containing avian collagen as a dermal equivalent (DE) ingredient, fibroblast proliferation increased by about 60% relative to the control model. Immunohistochemical staining showed that the expression of proliferating cell nuclear antigen (PCNA) and p63 increased in the avian collagen models, while the expression of involucrin, integrin α6, and integrin β1 remained unchanged. Next, DEs were cryopreserved to allow the easier creation of SEs. Keratinocytes were seeded on thawed DEs, and SEs were constructed. Avian collagen increased the viability of DEs relative to the control. Furthermore, avian collagen increased the expression of PCNA and p63 in keratinocytes on thawed DEs. The results indicate that DEs containing avian collagen can be thawed as needed after cryopreservation. Avian collagen can improve the construction of SEs and be used as part of a dermal kit for SE construction.

Effect of natural antioxidants and apocynin on LPS-induced endotoxemia in rabbit

2000 ◽  
Vol 19 (11) ◽  
pp. 604-614 ◽  
Author(s):  
V Ben-Shaul ◽  
L Lomnitskil ◽  
A Nyska ◽  
M Carbonatto ◽  
S Peano ◽  
...  
Keyword(s):  
Nadph Oxidase ◽  
Proliferating Cell Nuclear Antigen ◽  
Immunohistochemical Staining ◽  
Microsomal Fraction ◽  
Natural Antioxidants ◽  
Treated Group ◽  
Nuclear Antigen ◽  
Sod Activity ◽  
Proliferative Effect ◽  
Proliferating Cell

The objective of this study was to compare the prophylactic effects of the natural antioxidant from spinach (NAO) and apocynin, on the hepatic oxidative stress and liver damage induced by lipopolysaccharide (LPS). Male New Zealand rabbits were challenged with LPS with or without 8 days of antioxidant pretreatment. Pretreatment with NAO, but not apocynin, significantly (p<0.05) decreased the levels of hydroperoxides and malondialdehyde (MDA) in the liver cytosolic fraction and the activity of NADPH oxidase-generated superoxide in the microsomal fraction, compared to LPS alone. The activity of glutathione peroxidase (G-POX) was significantly (p<0.05) increased in the LPS-treated group, whereas treatment with NAO, but not apocynin, significantly (p< 0.05) decreased G-POX activity. Pretreatment with the same antioxidants had no significant effects on superoxide dismutase (SOD) activity, whereas an increased level of catalase (CAT) was obtained in all LPS-treated groups. TUNEL immunohistochemical staining in the LPS-treated animals indicated that there was no increase in apoptosis outside of necrotic foci. However, apoptotic hepatocytes were observed within areas of focal necrosis in animals exposed to LPS alone or LPS plus apocynin. Hepatocyte cell proliferation was tested by the proliferating-cell nuclear antigen (PCNA) tool, which indicated a proliferative effect in the LPS group, whereas the effect disappeared in the antioxidant-treated groups. The prophylactic effect of NAO on liver pathology and the significant decreases in lipid peroxidation products and NADPH oxidase activity suggest the use of NAO as an efficient strategy for treatanent of endotoxemia.

scholarly journals Association of MMP-9 and PCNA Protein Expression in Osteosarcoma are Associated with Clinical Stage, Metastasis, and Prognosis

2021 ◽  
Vol 21 (1-3) ◽  
Author(s):  
Bo Zhao
Keyword(s):  
Matrix Metalloproteinase ◽  
Proliferating Cell Nuclear Antigen ◽  
Immunohistochemical Staining ◽  
Clinical Stage ◽  
Nuclear Antigen ◽  
Upward Trend ◽  
Expression Levels ◽  
Enneking Stage ◽  
Proliferating Cell ◽  
Metalloproteinase 9

ABSTRACT Present work studies the expressions of MMP-9 and PCNA in osteosarcoma patients and its correlation with clinical stage. 53 specimens of osteosarcoma were surgically removed and 28 osteochondroma tissues were used for immunohistochemical staining to detect the expression of proliferating cell nuclear antigen (PCNA) as well as matrix metalloproteinase-9 (MMP-9). The expression rates of MMP-9, as well as PCNA, were 75.5 percent and 86.8 percent in osteosarcoma, while were 10.7 percent and 7.5 percent in osteochondroma, respectively, which was statistically significant (P<0.05). The expression levels of MMP-9, PCNA, and the total survival time were connected with the Enneking stage. It shows an upward trend for the expression levels of PCNA and MMP- 9 in osteosarcoma tissues. The expression levels of MMP-9 and PCNA are related to the clinical stage, metastasis, and prognosis.

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