Abstract 241: Endothelial Function is Preserved with Endoscopic Vein Harvest for Lower Extremity Bypass

2015 ◽  
Vol 35 (suppl_1) ◽  
Author(s):  
Adam R Wheeler ◽  
Daniel E Kendrick ◽  
Matthew T Allemang ◽  
Andre F Gosling ◽  
Anil Nagavalli ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Lower Extremity ◽  
Endothelial Function ◽  
Endothelial Damage ◽  
Krebs Solution ◽  
Force Transducers ◽  
Submaximal Contraction ◽  
Harvesting Techniques ◽  
Harvest Techniques ◽  
Bypass Technique

Objective: Endoscopic vein harvest for lower extremity arterial bypass technique has been questioned due to concern for endothelial damage during procurement. We sought to compare NO mediated endothelial dependent relaxation (EDR) in vein segments harvested with open surgical (OH) versus endoscopic (EH) techniques. Methods: Saphenous vein segments were harvested for lower extremity bypass. 3-4mm vein rings were mounted on force transducers. Segments were mounted in 37° oxygenated Krebs solution and maximally contracted using KCl. NE was used to achieve submaximal contraction. EDR was determined using increasing concentrations of bradykinin (BDK). Endothelial independent relaxation was confirmed using sodium nitroprusside. Two-way ANOVA was used to analyze differences between harvest techniques across BDK concentration. Student t-test was used to examine nitrite levels in each cohort. Results: Vein segments harvested from patients (n=13) led to 28 rings (11 rings; 5 patients EH v. 17;8 OH). Both cohorts achieved moderate relaxation to maximal BDK concentration, [10 -6 M]; (49.5% EH vs. 40.55%, OH, P = .270). Analysis by two way ANOVA for mean % relaxation for BDK concentration [10 -11 - 10 -6 M] showed improved EDR in EH samples compared to OH (P =.029). Mean nitrite tissue bath concentration measurements post-BDK were 279 nM (EH) v. 194 nM (OH) (P = .264). Histology and IHC confirmed intact endothelium by morphometric analysis and CD31 staining. Conclusion: Endothelial function is preserved when utilizing endoscopic harvesting techniques. The advantages of minimally invasive vein procurement for lower extremity bypass can be obtained without concern for damaging venous endothelium.

scholarly journals Endothelial function of human vena saphena magna prepared with different minimally invasive harvesting techniques

2000 ◽  
Vol 18 (4) ◽  
pp. 400-403 ◽  
Author(s):  
Alexander M. Fabricius ◽  
Andreas Oser ◽  
Anno Diegeler ◽  
Thomas Rauch ◽  
Friedrich W. Mohr
Keyword(s):  
Minimally Invasive ◽  
Endothelial Function ◽  
Vena Saphena Magna ◽  
Harvesting Techniques

scholarly journals eComment: Minimally invasive endoscope-enhanced venous conduit harvesting techniques

2010 ◽  
Vol 10 (4) ◽  
pp. 629-630
Author(s):  
Jamshid H. Karimov ◽  
Kakhaber Latsuzbaia ◽  
Mattia Glauber
Keyword(s):  
Minimally Invasive ◽  
Harvesting Techniques

Lung damage and pulmonary uptake of serotonin in intact dogs

1985 ◽  
Vol 58 (6) ◽  
pp. 1761-1766 ◽  
Author(s):  
C. A. Dawson ◽  
C. W. Christensen ◽  
D. A. Rickaby ◽  
J. H. Linehan ◽  
M. R. Johnston
Keyword(s):  
Oleic Acid ◽  
Endothelial Function ◽  
Glass Bead ◽  
Endothelial Damage ◽  
Lung Damage ◽  
Serotonin Uptake ◽  
Pulmonary Uptake ◽  
Endothelial Surface ◽  
Anesthetized Dogs ◽  
Extravascular Volume

We examined the influence of glass bead embolization and oleic acid, dextran, and imipramine infusion on the pulmonary uptake of trace doses of [3H]serotonin and the extravascular volume accessible to [14C]antipyrine in anesthetized dogs. Embolization and imipramine decreased serotonin uptake by 53 and 61%, respectively, but no change was observed with oleic acid or dextran infusion. The extravascular volume accessible to the antipyrine was reduced by 77% after embolization and increased by 177 and approximately 44% after oleic acid and dextran infusion, respectively. The results suggest that when the perfused endothelial surface is sufficiently reduced, as with embolization, the uptake of trace doses of serotonin will be depressed. In addition, decreases in serotonin uptake in response to imipramine in this study and in response to certain endothelial toxins in other studies suggest that serotonin uptake can reveal certain kinds of changes in endothelial function. However, the lack of a response to oleic acid-induced damage in the present study suggests that serotonin uptake is not sensitive to all forms of endothelial damage.

scholarly journals PVSS6. In Vivo Assessment of Endothelial Function in Human Lower Extremity Arteries

2012 ◽  
Vol 55 (6) ◽  
pp. 6S
Author(s):  
Vikram S. Kashyap ◽  
Ryan O. Lakin ◽  
Lindsay Feiten ◽  
Paul Bishop ◽  
Timur P. Sarac
Keyword(s):  
Lower Extremity ◽  
Endothelial Function ◽  
In Vivo Assessment

Comparison of Minimally Invasive versus Conventional Open Harvesting Techniques for Iliac Bone Graft in Secondary Alveolar Cleft Patients

2011 ◽  
Vol 128 (2) ◽  
pp. 485-491 ◽  
Author(s):  
Sheel Sharma ◽  
Lisa F. Schneider ◽  
Jason Barr ◽  
Shahram Aarabi ◽  
Patricia Chibbaro ◽  
...  
Keyword(s):  
Minimally Invasive ◽  
Bone Graft ◽  
Iliac Bone ◽  
Alveolar Cleft ◽  
Iliac Bone Graft ◽  
Harvesting Techniques

scholarly journals Mid-term endothelial dysfunction post COVID-19

2021 ◽  
Vol 42 (Supplement_1) ◽  
Author(s):  
S Lampsas ◽  
E Oikonomou ◽  
G Siasos ◽  
N Souvaliotis ◽  
A Goliopoulou ◽  
...  
Keyword(s):  
Endothelial Function ◽  
Hospital Discharge ◽  
Cardiovascular Complications ◽  
Endothelial Damage ◽  
Control Group ◽  
Post Discharge ◽  
Reference Limit ◽  
Coronary Syndrome ◽  
Control Subjects ◽  
History Of

Abstract Introduction Cardiovascular complications of Coronavirus disease (COVID-19), resulting from the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), have been documented. Endothelium-induced “cytokine storm” in critically ill COVID-19 patients is one of the leading causes of morbidity and mortality. Vascular endothelial damage caused by COVID-19 emphasizes the crucial role of endothelium in COVID-19 clinical impact. Purpose To examine the mid-term (1-month) impact of COVID-19 in endothelial function. Methods In this case control study, 20 consecutive patients who were hospitalized for COVID-19 either on Intensive Care Unit (ICU) or non-ICU were examined one month following hospital discharge. In the control group we recruited 12 consecutive subjects from the outpatient cardiology clinic. Demographic and clinical data were collected, and endothelial function was evaluated by brachial artery flow-mediated dilation (FMD). Results There was no difference in age between COVID-19 patients and control subjects (66±12 years vs. 71±5 years, p<0.17), in male sex (63% vs. 54%, p=0.66) in history of diabetes mellitus (27% vs. 36%, p=0.64), hypertension (36% vs. 54%, p=0.39), cardiovascular disease (27% vs.18%, p=0.61). From the COVID-19 subjects 65% were overweight or obese. During their hospitalization [3 ICU (15%)/17 non-ICU (85%), mean days: 17±6.7], 4 (20%) of COVID-19 patients developed ARDS, while single cases of stress-induced cardiomyopathy, pulmonary embolism, and acute coronary syndrome were detected. One month post discharge D-dimers (0.71±0.55 μg/ml) levels were above upper reference limit. Importantly, FMD one month after hospital discharge date, was significantly impaired in the COVID-19 group (3.59±1.63% vs. 9.31±2.98%, p<0.001) compared to control group. Conclusion Post COVID-19 subjects one month post discharge have significant impaired endothelial function compared to control subjects. These findings highlight the significant interaction of COVID-19 with arterial endothelium and merit further research to conclude on the exact impact of vascular endothelium in physical history of SARS-CoV-2 infection. FUNDunding Acknowledgement Type of funding sources: None.

scholarly journals Downregulated GTCPH I/BH4 Pathway and Decreased Function of Circulating Endothelial Progenitor Cells and Their Relationship with Endothelial Dysfunction in Overweight Postmenopausal Women

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Ying Luo ◽  
Zhenhua Huang ◽  
Jinli Liao ◽  
Zhihao Liu ◽  
Xiaopeng Li ◽  
...  
Keyword(s):  
Endothelial Function ◽  
Postmenopausal Women ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Premenopausal Women ◽  
Endothelial Damage ◽  
Normal Weight ◽  
Vascular Endothelial ◽  
Vascular Endothelial Function ◽  
Endothelial Progenitor

Endothelial progenitor cells (EPCs) have endogenous endothelium-reparative potential, but obesity impairs EPCs. Overweight premenopausal women have a normal number of circulating EPCs with functional activity, but whether EPCs in overweight postmenopausal women can repair obesity-related endothelial damage requires further investigation. For this purpose, we examined the function and number of circulating EPCs, evaluated vascular endothelial function, and explored the underlying mechanism. Compared with normal weight or overweight age-matched men, postmenopausal women (overweight or normal weight) had a diminished number of circulating EPCs and impaired vascular endothelial function, as detected by flow-mediated dilatation. Moreover, GTCPH I expression and the nitric oxide level in overweight postmenopausal women and men were significantly decreased. Together, our findings demonstrate that the number or function of circulating EPCs and endothelial function, which is partially regulated by the GTCPH I/BH4 signaling pathway, is not preserved in overweight postmenopausal women. The GTCPH I/BH4 pathway in circulating EPCs may be a potential therapeutic target for endothelial injury in overweight postmenopausal women.

Sign in / Sign up

Export Citation Format

Share Document