scholarly journals Protein Kinase C-Delta Mediates Adventitial Cell Migration Through Regulation of Monocyte Chemoattractant Protein-1 Expression in a Rat Angioplasty Model

2012 ◽  
Vol 32 (4) ◽  
pp. 943-954 ◽  
Author(s):  
Yi Si ◽  
Jun Ren ◽  
Pu Wang ◽  
Debra L. Rateri ◽  
Alan Daugherty ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Cell Migration ◽  
Protein Kinase ◽  
Monocyte Chemoattractant Protein 1 ◽  
Protein Kinase C Delta ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Kinase C

scholarly journals Homocysteine stimulates nuclear factor κB activity and monocyte chemoattractant protein-1 expression in vascular smooth-muscle cells: a possible role for protein kinase C

2000 ◽  
Vol 352 (3) ◽  
pp. 817-826 ◽  
Author(s):  
Guoping WANG ◽  
Yaw L. SIOW ◽  
Karmin O
Keyword(s):  
Smooth Muscle ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Vascular Smooth Muscle Cells ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Kinase C

Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine that stimulates the migration of monocytes into the intima of arterial walls. Although many factors that induce MCP-1 expression have been identified, the effect of homocysteine on the expression of MCP-1 in atherogenesis and the underlying mechanisms are not entirely clear. The objective of the present study was to investigate the role of homocysteine in MCP-1 expression in human aorta vascular smooth-muscle cells (VSMCs). After VSMCs were incubated with homocysteine for various time periods, a nuclease protection assay and ELISA were performed. Homocysteine (0.05Ő0.2mM) significantly increased the expression of MCP-1 mRNA (up to 2.7-fold) and protein (up to 3.3-fold) in these cells. The increase in MCP-1 expression was associated with the activation of protein kinase C (PKC) as well as nuclear factor κB (NF-κB). Further investigation demonstrated that the activation of NF-κB was the result of a PKC-mediated reduction in the expression of inhibitory protein (IκBα) mRNA and protein in homocysteine-treated cells. Oxidative stress might also be involved in the activation of NF-κB by homocysteine in VSMCs. In conclusion, the present study has clearly demonstrated that the activation of PKC as well as superoxide production followed by activation of NF-κB is responsible for homocysteine-induced MCP-1 expression in VSMCs. These results suggest that homocysteine-stimulated MCP-1 expression via NF-κB activation may play an important role in atherogenesis.

2133-P: Protein Kinase C Delta Mediates Cytokine-Induced Apoptosis in the Pancreatic Beta Cell

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 2133-P
Author(s):  
NIKKI L. FARNSWORTH ◽  
ROBERT A. PISCOPIO ◽  
RICHARD K. BENNINGER
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Beta Cell ◽  
Pancreatic Beta Cell ◽  
Protein Kinase C Delta ◽  
Kinase C ◽  
P Protein ◽  
Induced Apoptosis
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