scholarly journals Gelatin Sheet Incorporating Basic Fibroblast Growth Factor Enhances Healing of Devascularized Sternum in Diabetic Rats

Circulation ◽  
2001 ◽  
Vol 104 (suppl_1) ◽  
Author(s):  
Atsushi Iwakura ◽  
Yasuhiko Tabata ◽  
Nobushige Tamura ◽  
Kazuhiko Doi ◽  
Kazunobu Nishimura ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Bone Mineral ◽  
Basic Fibroblast Growth Factor ◽  
Median Sternotomy ◽  
Diabetic Rats ◽  
Wound Complications ◽  
Diabetic Patients ◽  
Fibroblast Growth ◽  
Mineral Density

Background Poor healing of the sternum often limits the use of bilateral internal thoracic arteries (BITAs) after coronary bypass surgery in diabetic patients. We have reported that a gelatin sheet that incorporates basic fibroblast growth factor (bFGF) accelerates sternal healing after BITA removal in normal rats. This study evaluated the effects of the above method for sternal healing in diabetic animals. Methods and Results Diabetic Wistar rats with blood glucose levels >400 mg/dL and body-weight loss >20 g were established by a single intravenous injection of streptozotocin (55 mg/kg). After median sternotomy and BITA removal, 16 diabetic rats received either a gelatin sheet that incorporated bFGF (100 μg/sheet) on the posterior table of the sternum (FGF group, n=9) or no gelatin sheet (control, n=7). Peristernal blood flow, as measured by a noncontact laser Doppler 4 weeks after surgery in the FGF group, recovered to the preoperative level (106±10% versus 82±9%, P <0.01), and marked angiogenesis was also observed around the sternum in the FGF group (30.5±3.2 versus 15.8±2.7 vessels/unit area, P <0.01). Deep sternal wound complications developed in 5 control rats but only in 1 rat in the FGF group ( P <0.05). In the FGF group, histological examination showed improved sternal healing (excellent in 6 rats and slow/poor healing in 3). Bone mineral content as assessed by dual-energy x-ray absorptometry was greater in the FGF group (75.9±18.1 versus 48.9±10.7 mg, P <0.05). Bone mineral density of the sternum was similar between the 2 groups. Conclusions A gelatin sheet that incorporates bFGF may offset sternal ischemia and accelerate sternal bone regeneration and healing, even in diabetic patients.

Novel Method to Enhance Sternal Healing After Harvesting Bilateral Internal Thoracic Arteries With Use of Basic Fibroblast Growth Factor

Circulation ◽  
2000 ◽  
Vol 102 (suppl_3) ◽  
Author(s):  
Atsushi Iwakura ◽  
Yasuhiko Tabata ◽  
Manabu Miyao ◽  
Makoto Ozeki ◽  
Nobushige Tamura ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Bypass Surgery ◽  
Active Form ◽  
Coronary Bypass ◽  
Coronary Bypass Surgery ◽  
Diabetic Patients ◽  
Fibroblast Growth ◽  
Group A

Background —Poor healing of the sternum often limits the use of bilateral internal thoracic arteries (BITAs) in coronary bypass surgery, especially for diabetic patients. We have reported that basic fibroblast growth factor (bFGF) enhanced regeneration of the skull. This study was designed to evaluate the effects of topical use of bFGF on sternal healing after removing the BITAs. Methods and Results —Forty-five Wistar rats were subjected to median sternotomy and were divided into 3 groups: 15 had the BITAs removed and had a bFGF sheet applied on the posterior table of the sternum (group A), 15 had just the BITAs removed (group B), and 15 had intact BITAs (group C). Five and 10 rats were euthanized 2 and 4 weeks after surgery, respectively, in all 3 groups. Peristernal blood flow, measured with use of a noncontact laser flowmeter, decreased after removal of the BITAs ( P <0.001). Four weeks after the surgery, PBF markedly increased only in group A (9.7±1.2, 6.5±0.6, and 8.2±0.5 mL · min −1 · 100 g −1 for groups A, B, and C, respectively; P <0.01 by ANOVA). Four weeks after surgery, the following findings were obtained only in group A: (1) nearly completely healed sternum filled with regenerated bone tissue, (2) marked angiogenesis around the sternum, and (3) osteoblasts in an active form around the edge of the sternum. Conclusions —The results suggest that use of the bFGF sheet offset the sternal ischemia and accelerated sternal healing. This method may help to decrease sternal necrosis in high-risk patients or allow extended use of BITAs in coronary bypass surgery.

Gelatin Sheet Incorporating Basic Fibroblast Growth Factor Enhances Healing of Devascularized Sternum in Diabetic Rats

Circulation ◽  
2001 ◽  
Vol 104 (suppl 1) ◽  
pp. I-325-I-329 ◽  
Author(s):  
Atsushi Iwakura ◽  
Yasuhiko Tabata ◽  
Nobushige Tamura ◽  
Kazuhiko Doi ◽  
Kazunobu Nishimura ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Diabetic Rats ◽  
Fibroblast Growth

scholarly journals A common variant in fibroblast growth factor binding protein 1 (FGFBP1) is associated with bone mineral density and influences gene expression in vitro

Bone ◽  
2010 ◽  
Vol 47 (2) ◽  
pp. 272-280 ◽  
Author(s):  
Nicole Hoppman ◽  
John C. McLenithan ◽  
Daniel J. McBride ◽  
Haiqing Shen ◽  
Jan Bruder ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Mineral Density ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Bone Mineral ◽  
Common Variant ◽  
Fibroblast Growth ◽  
Mineral Density ◽  
Factor Binding
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