Bursaphelenchus parayongensis n. sp. (Tylenchina: Aphelenchoididae) found in packaging wood from China

Nematology ◽  
2021 ◽  
pp. 1-13
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Ute Schönfeld ◽  
Xinxin Ma ◽  
Xiaoling Lü
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Excretory Pore ◽  
Posterior Margin ◽  
Posterior Part ◽  
Precloacal Papilla ◽  
Lateral Field ◽  
The Status ◽  
A New Species ◽  
Partial 18S

Summary Bursaphelenchus parayongensis n. sp. was isolated from packaging wood of a consignment from China exported to Germany. The new species is characterised by the lateral field with three lines, 9-13 μm long stylet with small basal swellings, and the excretory pore located near the posterior part of metacorpus. The male spicules are mitten-shaped, the lamina smoothly tapers towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular with sharply pointed tip, and without cucullus. One precloacal papilla, one adcloacal pair and two postcloacal pairs of male papillae present, with the posteriormost pair forming ‘gland papillae’. Bursal flap long, spade-like with posterior margin truncate or irregular. Females have a small vulval flap of 2-3 μm long, and a post-vulval uterine sac extending for 55-77% of the vulva to anus distance. Tail cylindrical, mostly (about 70%) with a robust mucron, very short to less than 3 μm, but occasionally tail terminus bluntly rounded, without mucron. It is similar to species in subgroup 3 of the eggersi group including B. carpini, B. clavicauda, B. cryphali and B. yongensis, but morphologically different. Detailed phylogenetic analysis based on partial 18S, ITS and D2-D3 region of 28S sequences has confirmed the status of this nematode as a new species.

Description of Ruehmaphelenchus taedae n. sp. (Nematoda: Aphelenchoididae) found in Loblolly pine logs from the USA

Nematology ◽  
2021 ◽  
pp. 1-10
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Xinxin Ma ◽  
Xiaoling Lü ◽  
Xianfeng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Loblolly Pine ◽  
Excretory Pore ◽  
Lateral Field ◽  
Female Tail ◽  
The Status ◽  
The Usa ◽  
Males And Females ◽  
A New Species

Summary Ruehmaphelenchus taedae n. sp., isolated from Loblolly pine logs (Pinus taedae L.) from the USA, is described and figured. It is characterised by a relatively slim body (a = 42 and 43 for males and females, respectively), three lines in the lateral field, male spicules relatively small (12-18 μm) with high and dorsally bent condylus and weakly developed rostrum, bursal flap absent, short tail possessing a long terminal spike ending in a bluntly rounded tip and 8.7-13.3 μm long, vulva positioned at ca 83% of body length, vulval flap absent, vulval lips slightly protruding, post-vulval uterine branch extending for less than half of vulva to anus distance, and female tail conoid, ca 3-4 anal body diam. long, with 13.7-18.5 μm terminal projection. The new species can be separated from all other species of the genus by the male tail possessing a long terminal spike and the more anterior excretory pore. Detailed phylogenetic analysis based on 28S D2-D3 region sequences confirmed the status of this nematode as a new species.

Description of Ruehmaphelenchus quercophilus n. sp. (Nematoda: Aphelenchoididae) from dying oak, Quercus robur, in Poland

Nematology ◽  
2017 ◽  
Vol 19 (9) ◽  
pp. 1051-1064
Author(s):  
Munawar Maria ◽  
Jianfeng Gu ◽  
Marek Tomalak ◽  
Yiwu Fang ◽  
Hongmei Li
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Quercus Robur ◽  
Nerve Ring ◽  
Its Sequences ◽  
Lateral Lines ◽  
The Status ◽  
A New Species

Ruehmaphelenchus quercophilus n. sp., isolated from a dying oak, Quercus robur, in the State Forests, Łopuchówko District, Poland, is described and figured. It is characterised by three lateral lines, relatively stout body, excretory pore posterior to nerve ring, five caudal papillae, spicules relatively small (12-14 μm) with weakly developed condylus and rostrum, bursa absent, vulva positioned at ca 78% of body length, vulval flap absent, post-uterine branch extending for slightly over half of vulva to anus distance, and conoid tail with a bend at the distal end forming a terminal projection 10-14 μm long. The new species is very similar to four other described species, namely R. formosanus, R. juliae, R. thailandae, and R. sirisus. Detailed phylogenetic analysis based on 18S, 28S D2-D3 and ITS sequences has confirmed the status of this nematode as a new species.

Bursaphelenchus chengi sp. n. (Nematoda: Parasitaphelenchidae) isolated at Nanjing, China, in packaging wood from Taiwan

Nematology ◽  
2008 ◽  
Vol 10 (3) ◽  
pp. 335-346 ◽  
Author(s):  
Hongmei Li ◽  
Phap Quang Trinh ◽  
Lieven Waeyenberge ◽  
Maurice Moens
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Body Size ◽  
Posterior Margin ◽  
Petri Dish ◽  
Packaging Materials ◽  
Lateral Field ◽  
Female Tail ◽  
Domain Sequence ◽  
Lateral Lines

Abstract Bursaphelenchus chengi sp. n. is described and illustrated. Dauer juveniles were isolated from imported wood packaging materials from Taiwan to Nanjing Port, China. Bursaphelenchus chengi sp. n. was reared and maintained on Petri dish cultures of the fungus Botrytis cinerea. The new species is characterised by the medium body size in both sexes, the presence of only two incisures in the lateral field and the robust and strongly curved spicules. The spicule lamina is angular distally, the rostrum digitate and the condylus rounded. The tail is arcuate with a pointed terminus. The bursa is usually truncate with the posterior margin indented in some specimens or rounded with a fine axial point. Females have a small vulval flap formed by a short extension of the cuticle of the anterior lip, and a conical tail that gradually tapers to an almost straight or slightly recurved, pointed or rounded terminus. Because of the presence of two lateral lines, similar spicule shape, tapering female tail and the presence of a small vulval flap, B. chengi sp. n. should be grouped in the abietinus-group sensu Braasch. together with B. abietinus, B. antoniae, B. hellenicus, B. hylobianum and B. rainulfi. ITS-RFLP profiles support the proposal of the new species, and phylogenetic analysis of the 28S rDNA D2/D3 domain sequence places it close to B. antoniae and other species of the abietinus-group.

Bursaphelenchus paraleoni n. sp. (Tylenchina: Aphelenchoididae) intercepted in Loblolly pine logs imported into China from the USA

Nematology ◽  
2021 ◽  
pp. 1-11
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Lele Liu ◽  
Xinxin Ma ◽  
Xiaoling Lu
Keyword(s):  
New Species ◽  
Pinus Taeda ◽  
Loblolly Pine ◽  
Precloacal Papilla ◽  
Long Tail ◽  
Lateral Field ◽  
The Usa ◽  
Males And Females ◽  
A New Species ◽  
Huge Variation

Summary Bursaphelenchus paraleoni n. sp. was isolated from Loblolly pine, Pinus taeda, logs from the USA. The new species is characterised by a lateral field with three lines, 13-16 μm long stylet with small basal swellings, and the excretory pore located slightly posterior to the nerve ring. The male spicules are mitten-shaped, the lamina smoothly tapering towards the distal tip. The condylus is broadly rounded, with a small dorsally bent hook, rostrum triangular or conical with finely rounded tip, and cucullus absent. One midventral precloacal papilla, one adcloacal pair and two postcloacal pairs of genital papillae are present. The bursal flap is long and spade-like with the posterior margin truncate or irregular. Females have a small vulval flap 4-6 μm long, a post-vulval uterine sac extending for 34-49% of the vulva to anus distance, a very long tail with slight ventral curvature, mostly filiform and with a finely rounded terminus, occasionally bluntly rounded. Huge variation in the tails of males and females were observed in a culture. It is similar to species of B. eidmanni, B. leoni, and B. silvestris in the leoni-group but detailed phylogenetic analysis based on 18S, ITS and D2-D3 28S sequences confirmed it as a new species.

Pseudodactylaria brevis sp. nov. from Thailand confirms the status of Pseudodactylariaceae

Phytotaxa ◽  
2018 ◽  
Vol 369 (4) ◽  
pp. 241 ◽  
Author(s):  
CHUAN-GEN LIN ◽  
ERIC H. C. MCKENZIE ◽  
DARBHE J. BHAT ◽  
JIAN-KUI LIU ◽  
KEVIN D. HYDE ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Sequence Data ◽  
Taxonomic Status ◽  
Its Sequence ◽  
Morphological Comparison ◽  
Phylogenetic Placement ◽  
Data Analyses ◽  
The Status ◽  
A New Species

A new species, Pseudodactylaria brevis, is described, illustrated and compared with other Pseudodactylaria and Dactylaria-like taxa. Evidence for the new species is provided by morphological comparison and sequence data analyses. Pseudodactylaria brevis can be distinguished from other Pseudodactylaria and Dactylaria-like species by its short hyaline conidiophores and fusiform, 1-septate hyaline conidia. Phylogenetic analysis of LSU and ITS sequence data was carried out to determine the phylogenetic placement of the species and confirm the taxonomic status of Pseudodactylariaceae.

Taking a closer look: an SEM review of Levinsenia species (Polychaeta: Paraonidae) reported from California

Zootaxa ◽  
2020 ◽  
Vol 4751 (2) ◽  
pp. 256-275
Author(s):  
LAWRENCE L. LOVELL ◽  
KIRK FITZHUGH
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Light Microscope ◽  
Molecular Phylogenetics ◽  
Range Extension ◽  
Sensory Organ ◽  
The Status ◽  
A New Species ◽  
Scanning Electron

Scanning electron (SEM) and light microscope examinations of members of Levinsenia Mesnil, 1897, species from California yielded a new species, new characters, emended name and range extension for L. kirbyorum Lovell, 2002. Specimens of L. gracilis (Tauber, 1879) from Sweden, Iceland, and California were compared and could not be distinguished on the basis of morphology. Two other Californian species, L. multibranchiata (Hartman, 1957) and L. oculata (Hartman, 1957), were also examined. SEM revealed features previously undescribed for the genus. Additional prostomial ciliary bundles, dorsal transverse ciliary branchial connections, notopodial sensory pores, and neurochaetal fascicle configurations. Levinsenia barwicki n.sp. possessing a terminal sensory organ, 4-8 leaf-like ciliate branchiae, and recurved neurochaete with distal hood is described More SEM work is necessary to confirm if these features are present among other members of Levinsenia and other Paraonidae genera. The status of Levinsenia according to the phylogenetic analysis performed by Langeneck et al. (2019, Molecular Phylogenetics and Evolution, 136, 1-13) is discussed. 

Bursaphelenchus pterocarpi n. sp. (Tylenchina: Aphelenchoididae) found in Pterocarpus sp. imported into China from Ghana

Nematology ◽  
2019 ◽  
Vol 21 (7) ◽  
pp. 725-737
Author(s):  
Jianfeng Gu ◽  
Yiwu Fang ◽  
Lele Liu ◽  
Majid Pedram ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Body Length ◽  
Excretory Pore ◽  
Reproductive System ◽  
Female Body ◽  
Nerve Ring ◽  
Wild Type ◽  
Lateral Field ◽  
Group Species ◽  
Partial 18S

Summary Bursaphelenchus pterocarpi n. sp. isolated from Pterocarpus sp. logs imported from Ghana to Ningbo, China, is described and illustrated. The new species is characterised by a female body length of 630-946 μm, lateral field with four lines, a short 12.6-13.9 μm long stylet with small basal swellings, excretory pore located posterior to nerve ring, outstretched reproductive system with amoeboid sperm inside the spermatheca, a well-developed vulval flap, tail conical and straight with a 1.9-4.8 μm long mucron at the tip, and lacking males in both the wild type and fungal-cultured populations. In a concatenated tree, reconstructed on the basis of the partial 18S, full length ITS and partial 28S rDNA D2-D3 sequences, the new species occupied a basal placement to the clade of xylophilus-group and africanus-group species.

Radopholus duriophilus sp. n. (Nematoda: Pratylenchidae) from Western Highland of Vietnam

Nematology ◽  
2003 ◽  
Vol 5 (4) ◽  
pp. 549-558 ◽  
Author(s):  
Chau Nguyen ◽  
Sergei Subbotin ◽  
Mehrdad Madani ◽  
Phap Trinh ◽  
Maurice Moens
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Sequence Divergence ◽  
Its Sequence ◽  
Entire Body ◽  
Lateral Field ◽  
Stylet Length ◽  
High Level ◽  
A New Species ◽  
Oval Shape

Abstract A new species of the genus Radopholus associated with durian (Durio zibetinus M.) in the Western Highland of Vietnam is described as Radopholus duriophilus sp. n. The new species is close to R. similis, but is distinguished from R. similis by the position of the excretory pore located posterior to pharyngo-intestine junction (vs at level of pharyngo-intestine junction), oval shape sperm (vs rod-like), four incisures terminating far behind position of phasmid (vs three incisures terminating at or just behind phasmid), and bursa in male never reaching tail terminus (vs bursa reaching tail terminus). Females of R. duriophilus sp. n. differ from R. nativus females by stylet length (16.5-19 vs 19-23 μm), oval or kidney-shaped sperm (vs rod-like), four incisures at level of phasmid (vs three) and their areolated lateral field (vs not areolated). The position of excretory pore of both female and male is located posterior to pharyngo-intestine junction (vs at level or anterior to pharyngo-intestine junction). Females of R. duriophilus sp. n. differ from R. clarus females by stylet length (16.5-19 vs 19-21 μm) and areolated lateral field (vs no areolation). Females of R. duriophilus sp. n. differ from R. musicola females by their lateral field with equidistant incisures at mid-body (vs two deep outer folds and two faint shallow inner incisures), oval or kidney-shaped sperm (vs rod-like), and rounded terminus tail (vs sharply pointed). The species also sp. n. differ in male stylet length (11.5-15 vs 8.8-12 μm). Females of R. duriophilus sp. n. differ from R. bridgei females by stylet length (16.5-19 vs 15-17.5 μm), median bulb length (11-16.5 vs 11-13 μm), length of hyaline tail (3-11 vs not more than 4 μm), and lateral field areolated for entire body (vs not areolated except irregularly on neck and tail). The male differs by stylet length (11.5-15 vs 10-12 μm) and length of the hyaline portion (4-9 vs 1-4 μm). In addition, the relatively high level of ITS sequence divergence of the new species from R. similis populations and the presence of nucleotide autapomorphies support a separate specific status for these durian populations. Results of surveys revealed that R. duriophilus sp. n. is rather widely distributed in durian orchards and associated with decline and death of trees in many durian nursery gardens. Densities of nematode population reached thousands of individuals per g of root samples.

Description of Aphelenchoides stellatus n. sp. (Nematoda: Aphelenchoididae) found in packaging wood from Japan

Nematology ◽  
2014 ◽  
Vol 16 (5) ◽  
pp. 621-630 ◽  
Author(s):  
Yiwu Fang ◽  
Jianfeng Gu ◽  
Xuan Wang ◽  
Hongmei Li
Keyword(s):  
New Species ◽  
Excretory Pore ◽  
Phylogenetic Analyses ◽  
Nerve Ring ◽  
Lateral Field ◽  
Male And Female ◽  
Female Tail ◽  
Morphological Status ◽  
Group 3 ◽  
A New Species

Aphelenchoides stellatusn. sp. is described and figured. The new species was isolated from packaging wood from Japan imported to Ningbo harbour, China. The new species has a body length of 485-533 μm (males) and 547-699 μm (females). The cuticle is weakly annulated and there are four lines in the lateral field. The stylet is 9-11 μm long and has small basal swellings. The excretory pore is located posterior to the nerve ring. Spicules smoothly curved, rose-thorn shaped. Apex and rostrum round, only slightly offset, dorsal limb 19-21 μm long. Male tail bearing six (2 + 2 + 2) caudal papillae. The female spermatheca is axial and oblong with round sperm present in multiple rows. Both male and female tail pegs have 3-4 processes, appearing star-like under SEM. The new species belongs to the Group 3 category ofAphelenchoidesspecies. Phylogenetic analyses based on full length ITS and 28S D2/D3 region of rDNA confirmed its morphological status as a new species.

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