Panagrellus levitatus sp. n. (Rhabditida: Panagrolaimidae), a nematode suppressing Drosophila melanogaster in laboratory cultures

A new nematode species recovered from the laboratory culture ofDrosophila melanogasteris described and illustrated. The mass reproduction ofPanagrellus levitatussp. n. in the fly culture occurred several times and resulted in a significant reduction of the fly population. Nematode outbreaks happened after the introduction ofD. melanogasterto the culture from natural sources. The new species is morphologically similar toP. ulmi. Partial LSU rDNA and SSU rDNA sequences were obtained and subjected to phylogenetic analysis that demonstrated the affinity of the new species withPanagrellussp. ‘MC2014’ from a red palm weevil. For the first time, the dauer juveniles ofPanagrelluswere described.

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Survey on diversity of marine/saline anaerobic Heterolobosea (Excavata: Discoba) with description of seven new species

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.063487-0 ◽

2014 ◽

Vol 64 (Pt_7) ◽

pp. 2280-2304 ◽

Cited By ~ 14

Author(s):

Tomáš Pánek ◽

Eliška Ptáčková ◽

Ivan Čepička

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Ssu Rdna ◽

Marine Environments ◽

Rdna Sequences ◽

The Family ◽

First Time

Diversity of the anaerobic Heterolobosea (Excavata: Discoba) is only poorly understood, especially in marine environments. We have isolated and cultured 16 strains of anaerobic heteroloboseid amoebae and flagellates from brackish, marine and saline anoxic habitats worldwide. Phylogenetic analyses of SSU rDNA sequences and light-microscopic observations showed that all the strains belong to the family Psalteriomonadidae, the main anaerobic lineage of Heterolobosea, and that they represent eight species from the genera Monopylocystis, Harpagon and Pseudoharpagon. Seven species are newly isolated and described here as Monopylocystis minor n. sp., Monopylocystis robusta n. sp., Monopylocystis elegans n. sp., Monopylocystis disparata n. sp., Harpagon salinus n. sp., Pseudoharpagon longus n. sp. and Pseudoharpagon tertius n. sp. Amoebae, cysts and the ultrastructure of the genus Pseudoharpagon are presented for the first time.

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Molecular and morphological characterisation of Cryptaphelenchus baujardi n. sp. (Nematoda: Ektaphelenchinae), a new species based on two populations from Iran

Nematology ◽

10.1163/15685411-00003194 ◽

2019 ◽

Vol 21 (1) ◽

pp. 45-56 ◽

Cited By ~ 1

Author(s):

Farzad Aliramaji ◽

Esmaeil Miraeiz ◽

Ebrahim Pourjam ◽

Ramin Heydari

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Lsu Rdna ◽

New Taxon ◽

Male Body ◽

Northern Iran ◽

Lateral Field ◽

Rdna Sequences ◽

Strong Resemblance ◽

Two Populations

Summary Cryptaphelenchus baujardi n. sp. is described and illustrated based on two populations from Golestan province, northern Iran. The new species is characterised by female and male body length of 224 (190-261) and 215 (195-229) μm, respectively, offset cephalic region with a shallow constriction, delicate stylet 6.6 (6.0-7.0) μm long, four incisures in the lateral field, presence of a rudimentary post-uterine sac in the female gonoduct, elongate-conoid female tail ending in an acute to finely rounded terminus, male tail conical, male with seven caudal papillae and delicate spicules. The new species shows a strong resemblance to C. varicaudatus and C. iranicus, but has a sclerotised mass near the tip of the spicule and an indistinct spermatheca. Phylogenetic analyses based on both partial SSU and LSU rDNA sequences confirm its status as a new taxon.

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DESCRIPTION OF MUCOR PERNAMBUCOENSIS (MUCORALES, MUCOROMYCOTA), A NEW SPECIES ISOLATED FROM THE BRAZILIAN UPLAND RAINFOREST

Phytotaxa ◽

10.11646/phytotaxa.350.3.6 ◽

2018 ◽

Vol 350 (3) ◽

pp. 274 ◽

Cited By ~ 1

Author(s):

CATARINA L. F. DE LIMA ◽

DIOGO X. LIMA ◽

CARLOS A. F. DE SOUZA ◽

RAFAEL J. V. DE OLIVEIRA ◽

INGRID B. CAVALCANTI ◽

...

Keyword(s):

New Species ◽

Molecular Data ◽

Lsu Rdna ◽

Rdna Sequences ◽

A New Species

The Mucor genus mostly comprises saprobe specimens which are commonly isolated from herbivore dung, fruit and soil. Morphologically, Mucor specimens produce simple or branched sporangiophores without basal rhizoids and non-apophysate sporangia. During a survey of Mucorales in soil from an upland rainforest area in Pernambuco, Brazil, one specimen of Mucor was isolated and characterized based on morphological, physiological and molecular data (ITS1-5.8S-ITS2 and LSU rDNA sequences). The specimen is characterized by producing strongly sympodially branched sporangiophores and sporangiospores which can contain one or more granules at each end. The columellae are frequently globose or obovoid, some ovoid or cylindrical with a truncate base, rarely piriform. It grew better at 25ºC, with no development at 35ºC. Based on the evidence of the analyzed datasets, a new species of Mucor is proposed.

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Phylogeny of dracunculoid nematodes (Chromadorea: Rhabditida: Spirurina: Dracunculoidea) from some Eurasian freshwater fishes

Zootaxa ◽

10.11646/zootaxa.4858.4.3 ◽

2020 ◽

Vol 4858 (4) ◽

pp. 521-541

Author(s):

SERGEY G. SOKOLOV ◽

ALEXANDER P. KALMYKOV ◽

SVETLANA V. MALYSHEVA

Keyword(s):

Phylogenetic Analysis ◽

Phylogenetic Relationship ◽

Ribosomal Dna ◽

Phylogenetic Relationships ◽

Type Species ◽

Freshwater Fishes ◽

Ssu Rdna ◽

Lsu Rdna ◽

Rdna Sequences ◽

The Family

Sets of small ribosomal DNA (SSU rDNA) and large ribosomal DNA (LSU rDNA) sequences were obtained for Philometroides moraveci Vismanis & Yunchis, 1994, Philometra kotlani (Molnár, 1969), Philometra rischta Skrjabin, 1923, Philometra cf. obturans (Prenant, 1886) (Philometridae), Sinoichthyonema amuri (Garkavi, 1972), Agrachanus scardinii (Molnár, 1966), Kalmanmolnaria intestinalis (Dogiel & Bychowsky, 1934) and Skrjabillanus tincae Shigin & Shigina, 1958 (Skrjabillanidae). Phylogenetic analysis of SSU rDNA data shows that dracunculoid nematodes are divided into two well-supported clades designated as Clade I and Clade II, respectively. Clade I includes the type species of the genus Philonema Kuitunen-Ekbaum, 1933, some species from the family Daniconematidae Moravec & Køie, 1987 and two subfamilies of skrjabillanids, Skrjabillaninae Shigin & Shigina, 1958 and Esocineminae Moravec, 2006. Clade II unites species from the families Dracunculidae Stiles, 1907, Micropleuridae Baylis & Daubney, 1926 and Philometridae Baylis & Daubney, 1926. Within the Philometridae, there are several well-supported groups of species, one of which unites freshwater Philometra spp. from the Palearctic cyprinids, identified as P. kotlani, P rischta, P. ovata (Zeder, 1803) and P. cyprinirutili (Creplin, 1825). However, the phylogenetic relationships of most philometrids are unresolved. An analysis of partial SSU and LSU rDNA sequences indicates that there is no direct phylogenetic relationship between Agrachanus Tikhomirova, 1971 (type species Skrjabillanus scardinii Molnár, 1966) and Skrjabillanus Shigin & Shigina, 1958 (type species Sk. tincae), which means that the genus Agrachanus can be resurrected. Our study confirms that Philonematinae Ivashkin, Sobolev & Khromova, 1971 should be elevated to the family rank. We formally establish the family Philonematidae Ivashkin, Sobolev & Khromova, 1971 stat. nov. We also suggest combining the superfamilies Dracunculoidea Stiles, 1907 and Camallanoidea Railliet & Henry, 1915 into the infraorder Camallanomorpha Roberts, Janovy & Nadler, 2013.

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Two new species and two new records of myxomycetes from subtropical forests in China

Phytotaxa ◽

10.11646/phytotaxa.350.1.6 ◽

2018 ◽

Vol 350 (1) ◽

pp. 51 ◽

Cited By ~ 1

Author(s):

YANG GAO ◽

SHU-ZHEN YAN ◽

GAO-WEI WANG ◽

SHUANG-LIN CHEN

Keyword(s):

New Species ◽

New Records ◽

Phylogenetic Analyses ◽

Diagnostic Feature ◽

Subtropical Forests ◽

Rdna Sequences ◽

Two New Species ◽

18S Rdna Sequences ◽

First Time ◽

Partial 18S

Two new myxomycetes from subtropical forests of China, Arcyria aeruginosa sp. nov. and Diderma subochraceum sp. nov., are described and illustrated. Arcyria aeruginosa occurred on rotten stem of bamboo and differs from all known species of the genus in having dense spines on inner surface of cups. The diagnostic feature of D. subochraceum is single-layered peridium which dehisces by longitudinal or stellate slit. Preliminary phylogenetic analyses based on partial 18S rDNA sequences support the establishment of the two new species. Additionally, Didymium saturnus H.W. Keller and Fuligo luteonitens L.G. Krieglst. & Nowotny are reported from China for the first time.

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Taxonomic Transfer of the Red Algal Genus Gloiosaccion to Chrysymenia (Rhodymeniaceae, Rhodymeniales), Including the Description of a New Species, Chrysymenia pseudoventricosa, for the Gulf of Mexico

Phytotaxa ◽

10.11646/phytotaxa.243.1.2 ◽

2016 ◽

Vol 243 (1) ◽

pp. 54 ◽

Cited By ~ 7

Author(s):

William E Schmidt ◽

Carlos Frederico D. Gurgel ◽

Suzanne L. Fredericq

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Gulf Of Mexico ◽

Caribbean Sea ◽

Distinct Species ◽

Lsu Rdna ◽

Rdna Sequences ◽

Red Algal ◽

Algal Genus ◽

A New Species

Gloiosaccion Harvey, with type G. brownii (Rhodymeniaceae, Rhodymeniales), is a red algal genus characterized by the presence of large, hollow and saccate vesicle-shaped thalli arising from small solid axes. Whereas Gloiosaccion has traditionally been viewed as being closely related to Botryocladia (Agardh) Kylin, a multi-marker phylogenetic analysis based on chloroplast-encoded rbcL and UPA and nuclear LSU rDNA sequences instead places Gloiosaccion brownii and G. pumila in the Chrysymenia clade that includes the generitype C. ventricosa (Lamouroux) J. Agardh. Gloiosaccion is reduced to synonymy with Chrysymenia J. Agardh, a taxonomic move first advocated by De Toni in 1900. In addition to C. brownii (Harvey) De Toni, “Gloiosaccion” brownii var. firmum Harvey and “G.” pumilum J. Agardh are recognized as the distinct species Chrysymenia coriacea comb. et stat. nov. and C. pumila (J. Agardh) Weber-van Bosse, respectively. A new species, C. pseudoventricosa sp. nov. is proposed to accommodate specimens going under the name C. ventricosa (J.V. Lamouroux) J. Agardh from the Gulf of Mexico and Caribbean Sea.

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A new species of Cryptomonas (Cryptophyceae) from the Western Urals (Russia)

European Journal of Taxonomy ◽

10.5852/ejt.2020.649 ◽

2020 ◽

Author(s):

Nikita A. Martynenko ◽

Evgeniy S. Gusev ◽

Pavel V. Kulizin ◽

Elena E. Guseva ◽

Kevin McCartney ◽

...

Keyword(s):

New Species ◽

Molecular Data ◽

Lsu Rdna ◽

Nuclear Rdna ◽

Ural Mountains ◽

Rdna Its2 ◽

Rdna Sequences ◽

Compensatory Base Changes ◽

Morphological And Molecular Data ◽

A New Species

A new species, Cryptomonas uralensis Martynenko, Gusev, Kulizin & Guseva sp. nov., is described from western slopes of the Ural Mountains (Russia) based on morphological and molecular data. Phylogenetic relationships inferred from nuclear SSU and LSU rDNA sequences show that the new species forms a clade with C. tetrapyrenoidosa Skuja emend. Hoef-Emden & Melkonian. Comparison of secondary structures of nuclear rDNA ITS2, including analysis of Compensatory Base Changes (CBC), confirms the separation between C. uralensis sp. nov. and C. tetrapyrenoidosa. Cell morphology and sizes of C. uralensis sp. nov. are very similar to C. tetrapyrenoidosa and C. pyrenoidifera, and C. uralensis sp. nov. may thus represent a species that can only be reliably identified using molecular data.

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Taxonomy and SSU rDNA-Based Phylogeny of Two Heterotrich Ciliates (Ciliophora, Heterotrichea) Collected From Subtropical Wetlands of China, Including the Description of a New Species, Linostomella pseudovorticella n. sp.

Frontiers in Microbiology ◽

10.3389/fmicb.2021.719360 ◽

2021 ◽

Vol 12 ◽

Author(s):

Didi Jin ◽

Xuetong Zhao ◽

Tingting Ye ◽

Jie Huang ◽

Alan Warren ◽

...

Keyword(s):

New Species ◽

Food Web ◽

Phylogenetic Analyses ◽

Small Subunit ◽

Ssu Rdna ◽

Microbial Food Web ◽

Model Organisms ◽

Rdna Sequences ◽

The Family ◽

A New Species

The Heterotrichea Stein, 1859 are a group of ciliated protists (single-celled eukaryotes) that occur in a wide variety of aquatic habitat where they play important roles in the flow of nutrients and energy within the microbial food web. Many species are model organisms for research in cytology and regenerative biology. In the present study, the morphology and phylogeny of two heterotrich ciliates, namely, Linostomella pseudovorticella n. sp. and Peritromus kahli Villeneuve-Brachon, 1940, collected from subtropical wetlands of China, were investigated using morphological and molecular methods. L. pseudovorticella n. sp. differs from its only known congener, Linostomella vorticella Ehrenberg, 1833 Aescht in Foissner et al., 1999, by having more ciliary rows (48–67, mean about 56 vs. 26–51, mean about 42) and its small-subunit (SSU) rDNA sequence, which shows a 15-bp divergence. Although P. kahli has been reported several times in recent decades, its infraciliature has yet to be described. A redescription and improved diagnosis of this species based on a combination of previous and present data are here supplied. Phylogenetic analyses based on SSU rDNA sequences revealed that the genus Linostomella is positioned within Condylostomatidae, and Peritromidae is sister to Climacostomidae with relatively low support, and the family Spirostomidae is the root branch of the class Heterotrichea.

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Characterisation of a population of Pratylenchus hippeastri from bromeliads and description of two related new species, P. floridensis n. sp. and P. parafloridensis n. sp., from grasses in Florida

Nematology ◽

10.1163/138855410x495809 ◽

2010 ◽

Vol 12 (6) ◽

pp. 847-868 ◽

Cited By ~ 13

Author(s):

Francesca De Luca ◽

Alberto Troccoli ◽

Larry W. Duncan ◽

Sergei A. Subbotin ◽

Lieven Waeyenberge ◽

...

Keyword(s):

New Species ◽

Molecular Level ◽

South Florida ◽

Rrna Gene ◽

Female Type ◽

Rdna Sequences ◽

Lesion Nematodes ◽

Type Population ◽

Root Lesion Nematodes ◽

First Time

Abstract Morphological and molecular analyses confirmed the presence of Pratylenchus hippeastri in regulatory samples collected in commercial bromeliad operations from genera Guzmania, Neoregelia and Vriesea in central and south Florida, USA. Specimens of P. hippeastri from bromeliads contained males which were not detected in the type population from amaryllis and are described herein for the first time. The rDNA sequences of these males matched those of P. hippeastri female type material. Pratylenchus hippeastri and root-lesion nematodes from several hosts in Florida were characterised at the morphological and molecular level, whereas other samples from Russia and South Africa were characterised at the molecular level only. Sequence and phylogenetic analysis using the ITS rRNA gene of these root-lesion nematodes revealed the presence of eight putative new species (spH1-H8) closely related to P. hippeastri. Here we describe two Florida representatives of the amphimictic root-lesion nematodes from Bahia grass (N1) and maidencane (N2), previously characterised by Inserra et al. in 1996 and Duncan et al. in 1999, as two new species phylogenetically related to P. hippeastri and named P. floridensis n. sp. and P. parafloridensis n. sp., respectively. The small round or oval (rarely rectangular and occasionally oblong) and enlarged spermatheca and the bluntly pointed or subacute tail with smooth and occasionally indented terminus separate P. floridensis n. sp. from P. parafloridensis n. sp., which has a quadrangular spermatheca and a subhemispherical or bluntly pointed tail with generally smooth and rarely indented terminus. However, these characters may overlap in some specimens making a morphological separation problematic without the use of molecular analysis. The close phylogenetic relationships shared by the species characterised in this study indicate that they are representatives of a P. hippeastri species complex.

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Monophyly of marsupial intraerythrocytic apicomplexan parasites from South America and Australia

Parasitology ◽

10.1017/s0031182009990710 ◽

2009 ◽

Vol 137 (1) ◽

pp. 37-43 ◽

Cited By ~ 5

Author(s):

SANTIAGO MERINO ◽

JAVIER MARTÍNEZ ◽

RODRIGO A. VÁSQUEZ ◽

JAN ŠLAPETA

Keyword(s):

South America ◽

Common Ancestor ◽

Phylogenetic Reconstruction ◽

Ssu Rdna ◽

Lsu Rdna ◽

South American ◽

Apicomplexan Parasites ◽

Rdna Sequences ◽

The Family ◽

Petaurus Australis

SUMMARYIntraerythrocytic parasites (Apicomplexa: Sarcocystidae) of the South American mouse opossum (Thylamys elegans) from Chile, South America, and of the yellow-bellied glider (Petaurus australis) from Australia were found to be monophyletic using SSU rDNA and partial LSU rDNA sequences. Phylogenetic reconstruction placed both species within the family Sarcocystidae. These intraerythrocytic parasites of marsupials represent an as yet unnamed genus predicted to have bisporocystic oocysts and tetrazoic sporocysts, which is a characteristic feature of all members of the family Sarcocystidae. These results show that erythrocytic parasites share a common ancestor and suggest co-evolution with their vertebrate host.

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