The article focuses on the functioning of the international universal institutional mechanism for the protection of human rights in business sphere. The importance of the statutory bodies of the United Nations, the officials who are empowered to make decisions on many issues related to a wide range of subjects of international legal relations, including the protection of human rights in business sphere, is emphasized, in particular: the General Assembly, the Economic and Social Council, the Security Council, the Secretary-General. The role of the Human Rights Council as a body of the United Nations, which is responsible for promoting the universal respect for human rights and fundamental freedoms, in the creation of specialized, narrow-profile human rights protection structures in business sphere has been defined. The powers of the Special Representative of the Secretary-General on Human Rights and transnational corporations and other business enterprises have been described, with an emphasis on the significance of “Protect, respect and remedy” framework proposed by him. The attention is paid to the Working Group on the issue of human rights and transnational corporations and other business enterprises. The importance of the UN Forum on Business and Human Rights as a dialogue center for the cooperation on business and human rights issues is highlighted. The emphasis is placed on the mandate of the Open-ended intergovernmental working group on transnational corporations and other business enterprises with respect to human rights, which provides the development of a legally binding instrument for regulating the activities of transnational corporations and other business enterprises in international human rights law. It is emphasized on the tasks performed by the Office of the United Nations High Commissioner for Human Rights in business and human rights issues, and its cooperation with specialized bodies in this area; its role in the promotion and implementation of the Guiding Principles on Business and Human Rights is highlighted.