A FIVE YEAR STUDY OF DST (DRUG SUSCEPTIBILITY TESTING) PATTERN OF M. TUBERCULOSIS ISOLATES FROM PATIENTS OF CATEGORY-II FAILURE OF PULMONARY TUBERCULOSIS UNDER DOTS (DIRECTLY OBSERVED TREATMENT, SHORT-COURSE) FROM NORTHERN INDIA.

The report of a case of tuberculous meningitis developing during the course of chemotherapy for pulmonary tuberculosis, by Lintermans and Seyhnaeve,1 illustrates the importance of drug susceptibility testing of the infecting organism. Initial cultures isolated from the gastric washings in this case were not tested for drug susceptibility, but subsequent cultures isolated from the spinal fluid after 33½ months of therapy proved to be isoniazid resistant. The authors state that "this complication (meningitis) developed despite specific chemotherapy and corticosteroids for active pulmonary tuberculosis."

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Evaluation of GenoType®MTBDRplus assay for rapid detection of drug susceptibility testing of multi-drug resistance tuberculosis in Northern India

Indian Journal of Pathology and Microbiology ◽

10.4103/0377-4929.118681 ◽

2013 ◽

Vol 56 (2) ◽

pp. 139 ◽

Cited By ~ 5

Author(s):

TapanN Dhole ◽

AnandKumar Maurya ◽

Jyoti Umrao ◽

AmreshKumar Singh ◽

Surya Kant ◽

...

Keyword(s):

Drug Resistance ◽

Rapid Detection ◽

Susceptibility Testing ◽

Drug Susceptibility ◽

Drug Susceptibility Testing ◽

Multi Drug Resistance ◽

Northern India

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Phenotypic characterisation and drug sensitivity testing of mycobacteria isolated from extra-pulmonary tuberculosis

Kathmandu University Medical Journal ◽

10.3126/kumj.v8i1.3223 ◽

1970 ◽

Vol 8 (1) ◽

pp. 57-61 ◽

Cited By ~ 3

Author(s):

R Gurung ◽

SK Bhattacharya ◽

B Pradhan ◽

S Gurung ◽

YI Singh

Keyword(s):

Developing Countries ◽

Drug Resistance ◽

Pulmonary Tuberculosis ◽

Susceptibility Testing ◽

Drug Susceptibility ◽

Drug Susceptibility Testing ◽

Phenotypic Characterization ◽

Extra Pulmonary Tuberculosis ◽

Extra Pulmonary

Background: Tuberculosis (TB) is a major cause of morbidity and mortality globally, with most cases occurring in developing countries. The importance of extra pulmonary tuberculosis (EPTB) among all forms of tuberculosis has not yet been ascertained in developing countries Objective: To identify and perform phenotypic characterization of clinically important mycobacterium isolates from extra-pulmonary sites along with drug susceptibility testing. Materials and methods: A total of 513 specimens from patients of (EPTB) with varied presentation were studied. Speciation and characterization of isolates were done on the basis of growth and biochemical characteristics. Drug susceptibility testing for mycobacterium isolates was done by proportion method. Results: Fifty four patients clinically suspected to have extra-pulmonary tuberculosis were culture positive for mycobacteria. On characterization 48 of the 54 isolates were identified as M. tuberculosis, 4 as M. bovis and 2 were M. avium/ intracelulare. Drug susceptibility testing was done for the both M. tuberculosis as well as M. bovis. In single drug resistance, Streptomycin was highest followed by Isoniazid, Ethambutol and Refampicin. Multi drug resistance (MDR) was found in 6 isolates and it was observed only in group I cases. Conclusion: EPTB accounts for 10-15 percent of all cases of tuberculosis. Mycobacterium was present in 10.5 percent samples. 48 isolates out of 54 samples were found to be M. tuberculosis. The maximum numbers of M. tuberculosis were isolated from lymph node aspiration. Key words: Phenotypic characterization; drug susceptibility; extra pulmonary tuberculosis DOI: 10.3126/kumj.v8i1.3223 Kathmandu University Medical Journal (2010), Vol. 8, No. 1, Issue 29, 57-61

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Direct drug susceptibility testing of Mycobacterium tuberculosis against primary anti-TB drugs in northern India

The Journal of Infection in Developing Countries ◽

10.3855/jidc.1079 ◽

2010 ◽

Vol 4 (11) ◽

pp. 695-703 ◽

Cited By ~ 5

Author(s):

Anamika Gupta ◽

Shampa Anupurba

Keyword(s):

Mycobacterium Tuberculosis ◽

Susceptibility Testing ◽

Drug Susceptibility ◽

Drug Susceptibility Testing ◽

Inexpensive Method ◽

Sensitivity Testing ◽

Northern India ◽

Resource Limited ◽

Nitrate Reductase Assay ◽

Proportion Method

Introduction: The present study aimed to evaluate a rapid and inexpensive colorimetric nitrate reductase assay (NRA) performed directly on sputum specimens for detection of drug resistance in Mycobacterium tuberculosis (MTB). Methodology: A total of 55 sputum samples were decontaminated and processed by modified Petroff's method. A part of the resulting suspension was used to perform direct NRA (DNRA) and direct proportion method (DPM) analysis. Of the 55 samples, 45 could be used to compare the two methods. Indirect drug sensitivity testing (DST) was also done for 14 MTB strains. Results: Excellent agreement was found between DNRA and DPM testing with κ values of 1, 0.91, 0.91, and 1 for RIF, INH, STR and EMB respectively. The sensitivities and specificities of DNRA compared to that of DPM were observed to be 100 and 100%, 100 and 93%, 95 and 96%, 100 and 100 % for RIF, INH, STR, and EMB respectively. Comparing the results of DNRA, DPM and indirect NRA with those of the gold standard indirect PM for 14 MTB strains showed that sensitivities, specificities and percent agreements were 100, 100 and 100% for all four tested drugs. Results for most of the specimens (55.6%) were available in 21 days with DNRA. Conclusions: We have saved valuable time by omitting the pre-isolation step and conclude that DNRA is a rapid, accurate and inexpensive method for direct DST of MTB and may become an appropriate alternative method for the resource limited settings.

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