Vulcanochloris (Trebouxiales, Trebouxiophyceae), a new genus of lichen photobiont from La Palma, Canary Islands, Spain

Phytotaxa ◽  
2015 ◽  
Vol 219 (2) ◽  
pp. 118 ◽  
Author(s):  
Lucie Vančurová ◽  
Ondřej Peksa ◽  
Yvonne Němcová ◽  
Pavel Škaloud
Keyword(s):  
Geographical Distribution ◽  
Canary Islands ◽  
18S Rdna ◽  
New Genus ◽  
Phylogenetic Analyses ◽  
Its Rdna ◽  
Sister Relationship ◽  
La Palma ◽  
Chloroplast Morphology ◽  
Rbcl Sequences

This paper describes a new genus of lichen photobionts, Vulcanochloris, with three newly proposed species, V. canariensis, V. guanchorum and V. symbiotica. These algae have been discovered as photobionts of lichen Stereocaulon vesuvianum growing on slopes of volcanos and lava fields on La Palma, Canary Islands, Spain. Particular species, as well as the newly proposed genus, are delimited based on ITS rDNA, 18S rDNA and rbcL sequences, chloroplast morphology, and ultrastructural features. Phylogenetic analyses infer the genus Vulcanochloris as a member of Trebouxiophycean order Trebouxiales, in a sister relationship with the genus Asterochloris. Our data point to the similar lifestyle and morphology of these two genera; however, Vulcanochloris can be well distinguished by a unique formation of spherical incisions within the pyrenoid. Mycobiont specificity and geographical distribution of the newly proposed genus is further discussed.

scholarly journals Molecular phylogeny and ultrastructure of the lichen microalga Asterochloris mediterranea sp. nov. from Mediterranean and Canary Islands ecosystems

2015 ◽  
Vol 65 (Pt_6) ◽  
pp. 1838-1854 ◽  
Author(s):  
Patricia Moya ◽  
Pavel Škaloud ◽  
Salvador Chiva ◽  
Francisco J. García-Breijo ◽  
José Reig-Armiñana ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Phylogenetic Analyses ◽  
Culture Collection ◽  
Its Rdna ◽  
Lsu Rdna ◽  
Correct Identification ◽  
Holotype Specimen ◽  
Transmission Electron ◽  
Chloroplast Morphology ◽  
Microscopy Techniques

The microalgae of the genus Asterochloris are the preferential phycobionts in Cladonia, Lepraria and Stereocaulon lichens. Recent studies have highlighted the hidden diversity of the genus, even though phycobionts hosting species of the genus Cladonia in Mediterranean and Canarian ecosystems have been poorly explored. Phylogenetic analyses were made by concatenation of the sequences obtained with a plastid – LSU rDNA – and two nuclear – internal transcribed spacer (ITS) rDNA and actin – molecular markers of the phycobionts living in several populations of the Cladonia convoluta-Cladonia foliacea complex, Cladonia rangiformis and Cladonia cervicornis s. str. widely distributed in these areas in a great variety of substrata and habitats. A new strongly supported clade was obtained in relation to the previously published Asterochloris phylogenies. Minimum genetic variation was detected between our haplotypes and other sequences available in the GenBank database. The correct identification of the fungal partners was corroborated by the ITS rDNA barcode. In this study we provide a detailed characterization comprising chloroplast morphology, and ultrastructural and phylogenetic analyses of a novel phycobiont species, here described as Asterochloris mediterranea sp. nov. Barreno, Chiva, Moya et Škaloud. A cryopreserved holotype specimen has been deposited in the Culture Collection of Algae of Charles University in Prague, Czech Republic (CAUP) as CAUP H 1015. We suggest the use of a combination of several nuclear and plastid molecular markers, as well as ultrastructural (transmission electron and confocal microscopy) techniques, both in culture and in the symbiotic state, to improve novel species delimitation of phycobionts in lichens.

scholarly journals Three new species, one new genus and subfamily of Dorylaimida (de Man, 1876) Pearse, 1942, and revisions on the families Tylencholaimellidae Jairajpuri, 1964 and Mydonomidae Thorne, 1964 (Nematoda: Dorylaimida)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7541
Author(s):  
Wen-Jia Wu ◽  
Chun-Ling Xu ◽  
Hui Xie ◽  
Dong-Wei Wang
Keyword(s):  
New Species ◽  
18S Rdna ◽  
New Genus ◽  
Phylogenetic Analyses ◽  
Basal Part ◽  
Autonomous Region ◽  
Ningxia Hui Autonomous Region ◽  
The Family ◽  
Three New Species ◽  
De Man

Three new species of the order Dorylaimida (de Man, 1876) Pearse, 1942 were identified and described. Paratylencholaimus sanshaensis gen. nov. sp. nov. from Hainan is proposed as a new member of the family Tylencholaimellidae Jairajpuri, 1964. Paratylencholaimus gen. nov. is close to Phellonema Thorne, 1964 and Goferus Jairajpuri & Ahmad, 1992 but can be differentiated mainly by having basal part of odontophore rod-like and without knobs, and basal part of pharynx expanded gradually. Tylencholaimus zhongshanensis sp. nov. from Guangdong and Dorylaimoides shapotouensis sp. nov. from the Ningxia Hui Autonomous Region are also described herein. Phylogenetic analyses based on the 18S rDNA and the D2–D3 region of the 28S rDNA support that the three new species are valid. The classifications of the families Tylencholaimellidae and Mydonomidae Thorne, 1964 are revised mainly based on the analysis of the morphology of odontostyle and odontophore. After these revisions, Paratylencholaiminae subfam. nov. including Paratylencholaimus gen. nov. and Goferus is proposed. Athernema and Agmodorus of Tylencholaimellidae are transferred into Mydonomidae, and the subfamily Athernematinae of Tylencholaimellidae is dismissed. The main characteristics of the family Mydonomidae and Tylencholaimellidae are revised. Keys to the genera of Mydomonidae and Tylencholaimellidae are included.

scholarly journals Sigmolenchus sinuosus n. gen., n. sp. (Tylenchidae: Ecphyadophorinae), a new member of the family

Nematology ◽  
2020 ◽  
Vol 22 (9) ◽  
pp. 985-997
Author(s):  
Azadeh Gharahkhani ◽  
Ebrahim Pourjam ◽  
Joaquín Abolafia ◽  
Pablo Castillo ◽  
Majid Pedram
Keyword(s):  
New Genus ◽  
Phylogenetic Analyses ◽  
Cephalic Region ◽  
Slender Body ◽  
Mangrove Forests ◽  
Electron Microscopic ◽  
Sister Relationship ◽  
Lateral Field ◽  
Muddy Sediments ◽  
Amphidial Fovea

Summary Sigmolenchus n. gen., is proposed as a new member of the Tylenchidae. The new genus is proposed based upon light and scanning electron microscopic (LM and SEM) observations and phylogenetic analyses based upon small, large and internal transcribed spacer sequences of ribosomal DNA (SSU, LSU D2-D3, ITS rDNA) and mitochondrial cytochrome c oxidase I gene (COI mtDNA) sequences. It is characterised partly by its slender body, but mainly by having a dorso-ventrally flattened, smooth and elevated cephalic region, unique elongate sinuous amphidial openings, each appearing as a wavy slit with three peaks, originating from the vicinity of the labial plate, extending posteriorly and reaching and passing the first body annulus, and a spacious amphidial fovea. It has an anteriorly rounded outline under LM, knobbed stylet, its conus comprising about one-third of the total length, lateral field with a single band, metacorpus with a distinct valve, vulva with small lateral flaps, and males common with tylenchoidiform spicules. The new genus is morphologically compared with seven tylenchid genera, viz., Chilenchus, Ecphyadophoroides, Filenchus, Labrys, Lelenchus, Sakia, and Tenunemellus, mainly by having small slender body and dorso-ventrally flat cephalic region, and is placed under the subfamily Ecphyadophorinae because of its slender body, elevated smooth cephalic region, long amphidial openings and spacious amphidial fovea. It was recovered from marine muddy sediments taken 2 m deep in mangrove forests off southern Iran. Currently S. sinuosus n. gen., n. sp. is monotypic. In SSU phylogeny, the new genus formed an unsupported sister relationship with Filenchus discrepans, and in LSU phylogeny it occupied a placement inside a major clade including several Tylenchinae genera.

Morphology and phylogeny of Halamphora yongxingensis sp. nov. (Bacillariophyta), a new marine benthic diatom isolated from Yongxing Island, South China Sea

Phytotaxa ◽  
2015 ◽  
Vol 195 (1) ◽  
pp. 53 ◽  
Author(s):  
Hai-ying Jiang ◽  
Chao-qun Hu ◽  
Hai-peng Yang ◽  
Lv-ping Zhang ◽  
Peng-fei Peng ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
South China Sea ◽  
South China ◽  
18S Rdna ◽  
Phylogenetic Analyses ◽  
Light And Electron Microscopy ◽  
Benthic Diatom ◽  
Rbcl Gene ◽  
China Sea ◽  
Morphological Differences

Halamphora yongxingensis sp. nov., a marine benthic diatom isolated from an intertidal reef around the Yongxing Island, South China Sea (16° 58’ 43.3” N, 112° 14’ 41.7” E), is described in this study on the basis of light and electron microscopy. This diatom is also compared with related taxa such as Halamphora subturgida (Hustedt) Levkov and Amphora subtropica Wachnicka & Gaiser. In addition, phylogenetic analyses based on 18S rDNA and rbcL gene were also conducted. The results revealed that H. yongxingensis was clustered into the Halamphora clade, closely related to Halamphora montana (Krasske) Levkov. We discuss morphological differences between H. yongxingensis and H. montana.

Multigene phylogenetic analyses including diverse radiolarian species support the “Retaria” hypothesis — The sister relationship of Radiolaria and Foraminifera

2011 ◽  
Vol 81 (1-2) ◽  
pp. 32-42 ◽  
Author(s):  
Yoshiyuki Ishitani ◽  
Sohta A. Ishikawa ◽  
Yuji Inagaki ◽  
Masashi Tsuchiya ◽  
Kozo Takahashi ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Sister Relationship ◽  
Relationship Of

scholarly journals Paleomagnetism and40Ar/39Ar ages from La Palma in the Canary Islands

2000 ◽  
Vol 1 (9) ◽  
pp. n/a-n/a ◽  
Author(s):  
Lisa Tauxe ◽  
Hubert Staudigel ◽  
Jan R. Wijbrans
Keyword(s):  
Canary Islands ◽  
La Palma

Asian Betylobraconinae (Hymenoptera, Braconidae), with description of a new genus and phylogenetic affinities of the tribe Facitorini

2008 ◽  
Vol 39 (2) ◽  
pp. 133-154 ◽  
Author(s):  
Alberto Sáez ◽  
Kaoru Maeto ◽  
Alejandro Zaldivar-Riverón ◽  
Sergey Belokobylskij
Keyword(s):  
New Species ◽  
Type Species ◽  
New Genus ◽  
Phylogenetic Analyses ◽  
28S Rrna ◽  
Asian Region ◽  
Molecular Phylogenetic ◽  
Phylogenetic Affinities ◽  
One New Species ◽  
Rrna Sequences

AbstractThe taxonomy of the Asian genera of the subfamily Betylobraconinae, a small and understudied group within the hymenopteran family Braconidae, is revised. A new genus exclusively from the Asian region, Asiabregma gen. nov., containing three species (A. ryukyuensis sp. nov. (type species, Japan and Malaya), A. makiharai sp. nov. (Japan) and A. sulaensis (van Achterberg), comb. nov. (Indonesia)) is described. One new species of Aulosaphobracon, A. striatus sp. nov. from Vietnam, and one of Facitorus, F. amamioshimus sp. nov. from Japan, are also described. Based on molecular phylogenetic analyses using COI mtDNA and 28S rRNA sequences, the three genera previously placed in the tribe Facitorini, Facitorus, Conobregma and Jannya, together with Asiabregma gen. nov., are transferred to the rogadine tribe Yeliconini.

Grazing effects on species composition in different vegetation types (La Palma, Canary Islands)

2011 ◽  
Vol 37 (3) ◽  
pp. 230-238 ◽  
Author(s):  
J.R. Arévalo ◽  
L. de Nascimento ◽  
S. Fernández-Lugo ◽  
J. Mata ◽  
L. Bermejo
Keyword(s):  
Species Composition ◽  
Canary Islands ◽  
Vegetation Types ◽  
La Palma ◽  
Grazing Effects

Actin type I introns offer potential for increasing phylogenetic resolution in Asterochloris (Chlorophyta: Trebouxiophyceae)

2006 ◽  
Vol 38 (5) ◽  
pp. 435-440 ◽  
Author(s):  
Matthew P. NELSEN ◽  
Andrea GARGAS
Keyword(s):  
Population Genetics ◽  
Phylogenetic Analyses ◽  
Species Concepts ◽  
Its Rdna ◽  
Type I ◽  
Phylogenetic Resolution

Primers are presented to amplify an intron-containing portion of the actin type I locus from Asterochloris algae. The amount of variation and phylogenetic resolution provided by the ITS rDNA are compared with that from the actin locus. The number of variable and parsimony-informative sites within the actin locus was drastically higher than that of the ITS. Phylogenetic analyses of the ITS resulted in poorly resolved trees with low support values. In contrast, phylogenetic analyses of the actin locus yielded trees with better support and resolution. It is concluded that the actin type I introns will be useful for studies investigating species concepts and population genetics.

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