Liparis meihuashanensis, a new orchid species from Fujian, China: Evidence from morphological and molecular analyses

Phytotaxa ◽  
2017 ◽  
Vol 323 (2) ◽  
pp. 182
Author(s):  
SHI-MING FAN ◽  
JIANG-FENG LIU ◽  
JUN-WEN ZHAI ◽  
CHENG-ZI YANG ◽  
ZE-HAO HUANG

A new orchid species, Liparis meihuashanensis, from Fujian, China is described and illustrated based on morphological and molecular analyses. Detailed morphological comparisons indicate that L. meihuashanensis is similar to L. auriculata and L. pauliana, but it can be distinguished from them by the shorter inflorescence, triangular floral bract, and a lip with a truncate-emarginate and mucronate apex and 2 small subconical calli on contracted base. Molecular analyses based on nuclear ITS and plastid matK DNA sequence data support the L. meihuashanensis as a distinct species.

Phytotaxa ◽  
2018 ◽  
Vol 349 (3) ◽  
pp. 281
Author(s):  
JIANGFENG LIU ◽  
MINGHE LI ◽  
SIREN LAN ◽  
YICHI LIANG

Bulbophyllum yongtaiense, a new orchid species from Fujian, China, is described and illustrated based on morphological and molecular analyses. Detailed morphological comparisons indicate that B. yongtaiense is similar to B. kuanwuense, but it can be distinguished from longer scape, fimbriate-ciliate on margins of dorsal sepals and petals, petals with obtuse apex and a lip with finely papillose near base. Molecular analyses based on nuclear ITS and plastid matK, atpI-atpH, and trnL-F DNA sequence data support B. yongtaiense is genetically similar to B. hirundinis and B. pecten-veneris, but it can be distinguished from them by having shorter lateral sepals, petals with obtuse apex and a lip with finely papillose near base.


Phytotaxa ◽  
2015 ◽  
Vol 204 (4) ◽  
pp. 253 ◽  
Author(s):  
YONG-YU SU ◽  
YI-LANG HUANG ◽  
LI-JUN CHEN ◽  
PEI-WEN ZHANG ◽  
Zhong-Jian Liu ◽  
...  

A new orchid species, Liparis wenshanensis, discovered in Yunnan, China is described and illustrated in this study based on morphological and molecular analyses. A detailed comparison between the newly discovered orchid and other members of the genus, Liparis, was conducted. The new plant is characterized by the combination of the following features: a long rachis with 45 to 55 flowers; white sepals, petals and column; a greenish lip with a purplish center; strongly recurved and revolute dorsal sepals and petals; strongly recurved, oblong lateral sepals; a cordate lip that is strongly deflexed below the middle, with a two-lobed apex and a two-lobed callus at the base; an arcuate column with a lamella extending along the center almost to the stigma, and with a pair of broad wings toward the apex. These features distinguish the new orchid from all other known species of Liparis. We proceeded to a phylogenetic analysis to ascertain the systematic position of this enigmatic species. Molecular analyses based on nuclear ribosomal ITS and plastid matK DNA sequence data supports the recognition of L. wenshanensis as a distinct species.


Phytotaxa ◽  
2019 ◽  
Vol 387 (2) ◽  
pp. 149-157 ◽  
Author(s):  
GUO-QIANG ZHANG ◽  
GUI-ZHEN CHEN ◽  
LI-JUN CHEN ◽  
SI-REN LAN

A new orchid species, Cymbidium yunnanensis (Orchidaceae; Epidendroideae; Cymbidieae), from Yunnan Province, China, is described and illustrated. Based on morphological and molecular analyses, we demonstrate that this new species is similar to C. floribundum, but it differs in its smaller size, leaves 5–15 cm, shorter scape, white flowers with pale purplish markings on its sepals and petals, lip with purplish red blotch, petals narrowly falcate oblong and lip midlobe nearly round with column foot 1.5–2.0 mm long. Phylogenetic analyses based on nuclear ribosomal ITS and plastid (matK, rbcL) DNA sequence data support C. yunnanensis as a new species.


Phytotaxa ◽  
2018 ◽  
Vol 345 (1) ◽  
pp. 51 ◽  
Author(s):  
SIREN LAN ◽  
LIJUN CHEN ◽  
GUIZHEN CHEN ◽  
XINYI WU ◽  
WENHUI RAO ◽  
...  

A new orchid species, Cymbidium densiflorum, from Yunnan, China, is described and illustrated here based on morphological and molecular analyses. A detailed comparison between the newly discovered orchid and other members of Cymbidium was conducted. The new plant belongs to C. section Cymbidium and has a pendent inflorescence, thick-coriaceous flowers with two pollinia and rigid leaves, and it differs greatly from all other known species of this section by being a large plant bearing leaves with an acute, oblique apex and producing a long inflorescence with more than 30 purplish brown large flowers, in which the lateral sepals are oblique and narrowly elliptic with a tooth at the apex and narrowly ovate-elliptic petals. A molecular study based on the nuclear ribosomal ITS and plastid matK, rbcL, trnG, trnL and atpI DNA sequence data indicated that C. densiflorum is sister to C. paucifolium.


Phytotaxa ◽  
2020 ◽  
Vol 453 (3) ◽  
pp. 275-283
Author(s):  
YUTING JIANG ◽  
LIANG MA ◽  
RUQIANG LIN ◽  
YUANFANG XIONG ◽  
SHUZHEN JIANG ◽  
...  

A new orchid species, Cymbidium codonanthum, from Yunnan Province, China, is described and illustrated based on morphological and molecular analyses. A detailed comparison between the newly discovered orchid and other members of Cymbidium was performed. The new plant what is similar to C. cochleare, but it is characterized by several morphological features that distinguish it from the latter as well as all other known species of Cymbidium. A molecular study based on nuclear ribosomal ITS and plastid matK and rbcL DNA sequence data indicates that C. codonanthum is genetically distinct from other species and a member of Cymbidium section Cyperochis.


2011 ◽  
Vol 43 (6) ◽  
pp. 561-567 ◽  
Author(s):  
K. PAPONG ◽  
G. KANTVILAS ◽  
H. T. LUMBSCH

AbstractThe phylogenetic placement of the genus Maronina was studied, based chiefly on phenotypic characters such as thallus colour and anatomy, secondary chemistry, the anatomy of the excipulum and the ascus-type. DNA sequence data of mitochondrial and nuclear ribosomal loci from some of the species support the hypothesis that Maronina is nested within Protoparmelia. Hence, Maronina is reduced to synonymy with Protoparmelia. Comparison of genetic distances suggests that the two varieties within M. orientalis should be regarded as distinct species. Consequently, the new combinations Protoparmelia australiensis (Hafellner & R. W. Rogers) Kantvilas et al., P. corallifera (Kantvilas & Papong) Kantvilas et al., P. hesperia (Kantvilas & Elix) Kantvilas et al., P. multifera (Nyl.) Kantvilas et al., and P. orientalis (Kantvilas & Papong) Kantvilas et al. are proposed.


Phytotaxa ◽  
2014 ◽  
Vol 186 (1) ◽  
pp. 51 ◽  
Author(s):  
Qiu-Xiang Chen ◽  
Gui-Zhen Chen ◽  
Ming-He Li ◽  
Shi-Pin Chen

In this study, we describe a new orchid species, Goodyera malipoensis, from Yunnan, China. We have performed morphological and molecular analyses on this new species. A detailed comparison between the newly discovered orchid and other members of Goodyera was conducted. The new plant is characterized by having a dense brownish green pubescence on the peduncle. Its ovate-lanceolate petal is unique in Goodyera genus. The hypochile is deeply concave-saccate, and inside there are two papillose rows on each side. These features distinguish the new orchid from all other known species of Goodyera. The molecular study based on nuclear ribosomal ITS sequence data and morphological differences support G. malipoensis as a distinct species.


2014 ◽  
Vol 62 (3) ◽  
pp. 235 ◽  
Author(s):  
S. Safaei Chaei Kar ◽  
F. Ghanavati ◽  
M. R. Naghavi ◽  
H. Amirabadi-zade ◽  
R. Rabiee

Onobrychis, comprising more than 130 species, is a genus of the family Fabaceae. At this time, the interspecies relationship of this biologically important genus is still a subject of great discussion and debate. To help resolve this disagreement, we used molecular phylogeny to analyse internal transcribed spacer (ITS) and trnL–trnF sequences of 76 species of Onobrychis. Bayesian interference, maximum parsimony and maximum likelihood analyses of nuclear ITS and plastid trnL–trnF DNA sequence data generated trees with strong posterior probability for two groups: Onobrychis subgen. Sisyrosema (including: Heliobrychis, Hymenobrychis, Afghanicae and Anthyllium sections) along with Laxiflorae section in Group I and Onobrychis subgen. Onobrychis (except Laxiflorae section) in the other (Group II). The Laxiflorae section roots back to the ancestral node for Sisyrosema subgen. O. viciifolia (cultivated species), which is closely associated with O. cyri var. cyri, suggesting that the latter may be a wild progenitor of O. viciifolia. The present study supported the paraphyly of subgenera Onobrychis and Sisyrosema. The study proposed the paraphyletic nature of the sections Onobrychis, Dendrobrychis, Heliobrychis and Hymenobrychis. Together with our molecular phylogenetic analyses we present a review of Onobrychis morphology and discuss and compare our results with those of earlier morphological and molecular phylogenetic analyses.


Phytotaxa ◽  
2019 ◽  
Vol 408 (1) ◽  
pp. 77-84
Author(s):  
YING-LI PENG ◽  
ZHUANG ZHOU ◽  
SI-REN LAN ◽  
ZHONG-JIAN LIU

A new orchid species, Cymbidium jiangchengense, from Yunnan Province, China, is described and illustrated. Its distinctiveness is evaluated with morphology and molecular analyses. A detailed comparison between the newly discovered orchid and other members of Cymbidium was performed. The new plant was characterized by stem-like pseudobulbs, narrowly oblong leaves, coriaceous leaves with an acute apex, a 2-flowered inflorescence, a purplish pink flower, narrowly elliptic sepals, petals, a obovate-lanceolate lip with a cordate midlobe, a yellow central callus, and a disc with a trough shape longitudinal lamella from the base extending to the base of the midlobe and a lamellae apex inflated to form two calluses that are not confluent apically. These features distinguish this new orchid from all other known species of Cymbidium. A molecular study based on nuclear ribosomal ITS and plastid matK and rbcL DNA sequence data indicates that C. jiangchengense is a distinct species that sister to C. wadae and a member of section Eburnea, subgenus Cyperorchis.


2016 ◽  
Vol 54 (11) ◽  
pp. 2813-2819 ◽  
Author(s):  
Kerry O'Donnell ◽  
Deanna A. Sutton ◽  
Nathan Wiederhold ◽  
Vincent A. R. G. Robert ◽  
Pedro W. Crous ◽  
...  

Multilocus DNA sequence data were used to assess the genetic diversity and evolutionary relationships of 67Fusariumstrains from veterinary sources, most of which were from the United States. Molecular phylogenetic analyses revealed that the strains comprised 23 phylogenetically distinct species, all but two of which were previously known to infect humans, distributed among eight species complexes. The majority of the veterinary isolates (47/67 = 70.1%) were nested within theFusarium solanispecies complex (FSSC), and these included 8 phylospecies and 33 unique 3-locus sequence types (STs). Three of the FSSC species (Fusarium falciforme,Fusarium keratoplasticum, andFusariumsp. FSSC 12) accounted for four-fifths of the veterinary strains (38/47) and STs (27/33) within this clade. Most of theF. falciformestrains (12/15) were recovered from equine keratitis infections; however, strains ofF. keratoplasticumandFusariumsp. FSSC 12 were mostly (25/27) isolated from marine vertebrates and invertebrates. Our sampling suggests that theFusarium incarnatum-equisetispecies complex (FIESC), with eight mycoses-associated species, may represent the second most important clade of veterinary relevance withinFusarium. Six of the multilocus STs within the FSSC (3+4-eee, 1-b, 12-a, 12-b, 12-f, and 12-h) and one each within the FIESC (1-a) and theFusarium oxysporumspecies complex (ST-33) were widespread geographically, including three STs with transoceanic disjunctions. In conclusion, fusaria associated with veterinary mycoses are phylogenetically diverse and typically can only be identified to the species level using DNA sequence data from portions of one or more informative genes.


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