New insights on Hygrophorus penarioides and H. penarius (Agaricales, Hygrophoraceae) from Hungary

Phytotaxa ◽  
2019 ◽  
Vol 392 (2) ◽  
pp. 127
Author(s):  
ERIK ZAJTA ◽  
TORDA VARGA ◽  
GÁBOR M KOVÁCS ◽  
BÁLINT DIMA
Keyword(s):  
Sequence Variation ◽  
Geographical Origin ◽  
Phylogenetic Analyses ◽  
Morphological Variability ◽  
Strong Support ◽  
Morphological Evidence ◽  
Its2 Region ◽  
Herbarium Specimens ◽  
Nrdna Its ◽  
Nrdna Its Regions

The ectomycorrhiza forming basidiomycete Hygrophorus penarius sensu lato was recognized as two species, H. penarioides and H. penarius in 2007 based on molecular and morphological evidence. However, this was based on few collections of restricted geographical origin and no studies have performed molecular analysis on additional specimens to date.To this end we sequenced the nrDNA ITS regions of 29 H. penarius s. l. collections from Hungary and Slovakia and analysed with collections from previous studies. Bayesian and Maximum Likelihood phylogenetic analyses confirmed the separation of H. penarioides and H. penarius in two clades with the majority of the specimens belonging to H. penarioides and only three to H. penarius. Despite the strong support of the lineages, there was no coherent difference in basidiome and spore traits. We also provide the first evidence of an intraspecific nrDNA sequence variation in the ITS2 region of H. penarioides together with morphological variability that has not been reported to date.On the basis of our result, we conclude that the Quercus associated H. penarioides is far more common in Hungary than the Fagus associated H. penarius and we suggest that identification of the two species in the absence of DNA sequence analysis should be handled with caution, especially when herbarium specimens are examined.

scholarly journals Molecular and morphological evidence reveal a new genus and species in Auriculariales from tropical China

MycoKeys ◽  
2018 ◽  
Vol 35 ◽  
pp. 27-39 ◽  
Author(s):  
Hai-Sheng Yuan ◽  
Xu Lu ◽  
Cony Decock
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Type Species ◽  
New Genus ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Morphological Characteristics ◽  
Morphological Evidence ◽  
New Genus And Species ◽  
Tropical China

Grammatuslabyrinthinusgen. et sp. nov. is proposed based on DNA sequences data and morphological characteristics. It is known so far from southern, tropical China. The new species is characterised by an annual, resupinate basidiocarp with a shallow, subporoid hymenophore, a hymenium restricted to the bottom of the tubes, a dimitic hyphal system, presence of encrusted skeletocystidia and dendrohyphidia, longitudinally septate basidia and smooth, oblong-ellipsoid to cylindrical, acyanophilous basidiospores. Phylogenetic analyses based on ITS + nLSU DNA sequences data indicate that G.labyrinthinus belongs to Auriculariaceae in which it has an isolated position. Phylogenetic inferences show G.labyrinthinus to be related to Heteroradulum. However, the ITS sequences similarity between G.labyrinthinus and H.kmetii, the type species of Heteroradulum, were 89.84% and support the establishment of the new genus. Inversely, Heteroradulumsemis clustered with G.labyrinthinus with strong support and it is transferred to Grammatus.

An expanded circumscription for the previously monotypic Pleurospermopsis (Apiaceae) based on nrDNA ITS sequences and morphological evidence

Phytotaxa ◽  
2020 ◽  
Vol 460 (2) ◽  
pp. 129-136
Author(s):  
JING ZHOU ◽  
JIN WEI ◽  
ZHENWEN LIU
Keyword(s):  
Taxonomic Revision ◽  
Identification Key ◽  
Its Sequences ◽  
New Combination ◽  
Morphological Evidence ◽  
Herbarium Specimens ◽  
Nrdna Its

Under the framework phylogeny of Apiaceae subfamily Apioideae and through a carefully examination of herbarium specimens, a taxonomic revision for Pleurospermopsis (a genus originally accepted as monotypic to include P. sikkimensis as the only representative), is presented. Its circumscription is expanded to comprise Pleurospermum bicolor (Franch.) Norman ex Pan & Watson. Therefore, a new combination of Pleurospermopsis bicolor (Franch.) J. Zhou & J. Wei is proposed with full taxonomic treatments. An identification key is also provided for the genus.

A Phylogenetic Analysis and Taxonomic Revision of Bartonia (Gentianaceae: Gentianeae), Based on Molecular and Morphological Evidence

2009 ◽  
Vol 34 (1) ◽  
pp. 162-172 ◽  
Author(s):  
Katherine G. Mathews ◽  
Niall Dunne ◽  
Emily York ◽  
Lena Struwe
Keyword(s):  
Phylogenetic Analyses ◽  
Strong Support ◽  
Taxonomic Revision ◽  
Sister Group ◽  
Species Boundaries ◽  
Morphological Evidence ◽  
Phylogenetic Study ◽  
Sister Group Relationship ◽  
Internal Transcribed Spacer Region ◽  
Test Species

A phylogenetic study and taxonomic revision of the four currently accepted species of Bartonia (Gentianaceae, subtribe Swertiinae) were conducted in order to test species boundaries and interspecific relationships. Species boundaries were examined based on measurements of key quantitative and qualitative morphological characters as given in the original descriptions. Phylogenetic analyses were performed using molecular data from the nuclear internal transcribed spacer region and chloroplast DNA (trnL intron through the trnL-F spacer), separately and combined using parsimony and Bayesian methodologies, incorporating outgroups from subtribes Swertiinae and Gentianinae. The morphological study revealed that characters of one species, B. texana, represent a subset of the morphological variation found within B. paniculata, but that B. paniculata, B. verna, and B. virginica could all be separated from one another. The molecular phylogenetic analyses all found B. texana to nest in a clade with the two recognized subspecies of B. paniculata (subsp. paniculata and subsp. iodandra), making the latter paraphyletic. Bartonia texana is here reduced to subspecific rank, as Bartonia paniculata subsp. texana. Also, the phylogenetic analyses showed strong support for a sister group relationship between B. verna and B. virginica, as opposed to between B. paniculata and B. virginica as has been previously suggested.

Molecular and morphological evidence indicates that Pseudorhabdosynochus lantauensis (Monogenea: Diplectanidae) represents two species

Parasitology ◽  
2005 ◽  
Vol 130 (6) ◽  
pp. 669-677 ◽  
Author(s):  
X. Y. WU ◽  
N. B. CHILTON ◽  
X. Q. ZHU ◽  
M. Q. XIE ◽  
A. X. LI
Keyword(s):  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Strong Support ◽  
Morphological Characteristics ◽  
Sufficient Evidence ◽  
Bootstrap Support ◽  
Morphological Evidence ◽  
Lsu Rdna ◽  
Epinephelus Coioides

Sequences of the first internal transcribed spacer (ITS-1) and the D1-D3 domains of the large subunit (LSU) of the ribosomal DNA (rDNA) were determined for multiple specimens of 4 operational taxonomic units (OTUs) of the monogenean, Pseudorhabdosynochus lantauensis. OTUs were defined based on their collecting localities, host and/or morphological characteristics. All P. lantauensis specimens of one group (OTUs 1 and 3) differed in their sequences of the ITS-1 and partial LSU rDNA when compared with specimens of a second group (OTUs 2 and 4) by 12% and 2%, respectively. Results of the phylogenetic analyses of the LSU rDNA sequence data showed total (100%) bootstrap support for the separation of P. lantauensis into 2 distinct clades. At least 11 of the 18 nucleotide differences in the LSU sequence between the two P. lantauensis clades were derived (i.e. autapomorphic) characters when the morphologically distinct species, P. epinepheli and P. coioidesis, were used as outgroups. Furthermore, there were several autapomorphic character states for each P. lantauensis clade. This provides sufficient evidence to reject the null hypothesis that P. lantauensis represents a single species. Morphological and morphometric differences between these two clades provided additional strong support for the separation of P. lantauensis into two species. These two parasite species were found to co-exist on one of the two species of serranid fish (i.e. Epinephelus coioides) examined in the South China Sea (Guangdong Province, China).

Transfer of Lejeunea huctumalcensis to Physantholejeunea (Lejeuneaceae, Porellales)

2013 ◽  
Vol 26 (5) ◽  
pp. 386 ◽  
Author(s):  
Aleksandra Czumay ◽  
Shanshan Dong ◽  
Armin Scheben ◽  
Alfons Schäfer-Verwimp ◽  
Kathrin Feldberg ◽  
...  
Keyword(s):  
Chloroplast Genome ◽  
Phylogenetic Analyses ◽  
Morphological Evidence ◽  
Its2 Region ◽  
Rbcl Gene ◽  
Molecular Phylogenetic

Phylogenetic analyses of a three-marker dataset of Lejeuneaceae (chloroplast genome rbcL gene and trnL–trnF region, and nuclear ribosomal ITS1–5.8S-ITS2 region) resolve Lejeunea huctumalcensis (synonym Ceratolejeunea dussiana) in a well supported lineage with Physantholejeunea portoricensis. Representatives of Lejeunea and Ceratolejeunea form independent lineages. Physantholejeunea and L. huctumalcensis share the presence of ocelli, pycnolejeuneoid innovations and keeled perianths, with keels forming horn-like projections. On the basis of the molecular phylogenetic and morphological evidence, we transfer L. huctumalcensis to Physantholejeunea.

Size doesn´t matter—recircumscription of Microlejeunea (Lejeuneaceae, Porellales) based on molecular and morphological evidence

Phytotaxa ◽  
2013 ◽  
Vol 85 (2) ◽  
pp. 41 ◽  
Author(s):  
SHANSHAN DONG ◽  
ALFONS SCHÄFER-VERWIMP ◽  
TAMÁS PÓCS ◽  
KATHRIN FELDBERG ◽  
ALEKSANDRA CZUMAJ ◽  
...  
Keyword(s):  
Chloroplast Genome ◽  
Phylogenetic Analyses ◽  
Morphological Evidence ◽  
Its2 Region ◽  
Rbcl Gene ◽  
F Region

Phylogenetic analyses of a three marker dataset of Lejeuneaceae (chloroplast genome rbcL gene and trnL-F region, and nuclear ribosomal ITS1-5.8S-ITS2 region) resolve the “Lejeunea complex” in three main lineages assigned here to Harpalejeunea, Lejeunea and Microlejeunea. The taxa Harpalejeunea fischeri, H. filicuspis, H. latitans and Pluvianthus squarrosus are nested in a clade with several representatives of Microlejeunea including the generitype M. africana, and are transferred to the latter genus. Harpalejeunea and Microlejeunea differ from Lejeunea by the presence of ocelli. Harpalejeunea has diverging, blunt underleaf lobes in contrast to the forward directed, blunt to acute underleaf lobes of Microlejeunea. Morphologically similar accessions of Microlejeunea form independent lineages. Drepanolejeunea vandenberghenii is newly reported for Madagascar, Malawi and Réunion.

Phylogenetic analyses of New Caledonian Metrosideros and Carpolepis (Myrtaceae) from nrDNA (ITS) sequences

2000 ◽  
Vol 13 (6) ◽  
pp. 919 ◽  
Author(s):  
Shane D. Wright ◽  
D. Jeannette Keeling ◽  
Fern G. Ashton ◽  
John W. Dawson ◽  
Richard C. Gardner
Keyword(s):  
Ribosomal Dna ◽  
Phylogenetic Relationships ◽  
Sequence Variation ◽  
New Caledonia ◽  
Phylogenetic Analyses ◽  
Nuclear Ribosomal Dna ◽  
Nrdna Its ◽  
Large Measure ◽  
Dna Analyses ◽  
Morphological Taxonomy

We have analysed the phylogenetic relationships of all known species in the genera Metrosideros and Carpolepis (Myrtaceae) from New Caledonia. Variation in nucleotide characters from the internally transcribed spacers of nuclear ribosomal DNA was used to construct parsimony and neighbour-joining phylogenies. These show that there is a large measure of congruence between the existing morphological taxonomy for those species and the relationships inferred from analyses of sequence variation. However, the DNA analyses suggest that Carpolepis may have only equal ranking to the infrageneric Metrosideros clades. The DNA analyses also nest M. tetrasticha among the species of subg. Metrosideros, as opposed to its present classification as a monotypic section (Neocaledonica) within subg. Mearnsia. On the basis of these findings, and of the observed differentiation shown in the analyses between the sectional clades Calyptropetala and Mearnsia of subg. Mearnsia, equivalent rankings may be appropriate for four infrageneric clades in Metrosideros. These four are currently classified as the genus Carpolepis (three species), and subg. Metrosideros (seven species), subg. Mearnsia sect. Mearnsia (five species) and subg. Mearnsia sect. Calyptropetala (five species) within Metrosideros.

Diversification of the North American Doellingeria-Eucephalus Clade (Astereae: Asteraceae) Inferred from Molecular and Morphological Evidence

2019 ◽  
Vol 44 (4) ◽  
pp. 930-942
Author(s):  
Geraldine A. Allen ◽  
Luc Brouillet ◽  
John C. Semple ◽  
Heidi J. Guest ◽  
Robert Underhill
Keyword(s):  
North American ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
Morphological Evidence ◽  
South American ◽  
New Combinations ◽  
The North ◽  
Hybridization Event ◽  
Ancient Hybridization

Abstract—Doellingeria and Eucephalus form the earliest-diverging clade of the North American Astereae lineage. Phylogenetic analyses of both nuclear and plastid sequence data show that the Doellingeria-Eucephalus clade consists of two main subclades that differ from current circumscriptions of the two genera. Doellingeria is the sister group to E. elegans, and the Doellingeria + E. elegans subclade in turn is sister to the subclade containing all remaining species of Eucephalus. In the plastid phylogeny, the two subclades are deeply divergent, a pattern that is consistent with an ancient hybridization event involving ancestral species of the Doellingeria-Eucephalus clade and an ancestral taxon of a related North American or South American group. Divergence of the two Doellingeria-Eucephalus subclades may have occurred in association with northward migration from South American ancestors. We combine these two genera under the older of the two names, Doellingeria, and propose 12 new combinations (10 species and two varieties) for all species of Eucephalus.

Tracing the origins of hybrids through history: monstrous cultivars and Napoléon Bonaparte’s exiled paper daisies (Asteraceae; Gnaphalieae)

2021 ◽  
Author(s):  
Timothy L Collins ◽  
Jeremy J Bruhl ◽  
Alexander N Schmidt-Lebuhn ◽  
Ian R H Telford ◽  
Rose L Andrew
Keyword(s):  
Gene Flow ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Napoleon Bonaparte ◽  
Snp Data ◽  
Cast Doubt ◽  
Molecular Phylogenetic ◽  
St Helena ◽  
New Evidence

Abstract Golden everlasting paper daisies (Xerochrysum, Gnaphalieae, Asteraceae) were some of the earliest Australian native plants to be cultivated in Europe. Reputedly a favourite of Napoléon Bonaparte and Empress Joséphine, X. bracteatum is thought to have been introduced to the island of St Helena in the South Atlantic during Napoléon’s exile there. Colourful cultivars were developed in the 1850s, and there is a widely held view that these were produced by crossing Xerochrysum with African or Asian Helichrysum spp. Recent molecular phylogenetic analyses and subtribal classification of Gnaphalieae cast doubt on this idea. Using single-nucleotide polymorphism (SNP) data, we looked for evidence of gene flow between modern cultivars, naturalized paper daisies from St Helena and four Xerochrysum spp. recorded in Europe in the 1800s. There was strong support for gene flow between cultivars and X. macranthum. Paper daisies from St Helena were genotypically congruent with X. bracteatum and showed no indications of ancestry from other species or from the cultivars, consistent with the continuous occurrence of naturalized paper daisies introduced by Joséphine and Napoléon. We also present new evidence for the origin of colourful Xerochrysum cultivars and hybridization of congeners in Europe from Australian collections.

Molecular systematics of Thouarella (Octocorallia:Primnoidae) with the description of three new species from the Southern Ocean based on combined molecular and morphological evidence

2021 ◽  
Author(s):  
Mónica Núñez-Flores ◽  
Daniel Gomez-Uchida ◽  
Pablo J. López-González
Keyword(s):  
New Species ◽  
Southern Ocean ◽  
Species Delimitation ◽  
Nuclear Dna ◽  
Phylogenetic Analyses ◽  
Weddell Sea ◽  
Morphological Evidence ◽  
Three New Species ◽  
Taxonomic Framework ◽  
The Antarctic

Thouarella Gray, 1870, is one of the most speciose genera among gorgonians of the family Primnoidae (Cnidaria:Octocorallia:Anthozoa), being remarkably diverse in the Antarctic and sub-Antarctic seafloor. However, their diversity in the Southern Ocean is likely underestimated. Phylogenetic analyses of mitochondrial and nuclear DNA markers were integrated with species delimitation approaches as well as morphological colonial and polyps features and skeletal SEM examinations to describe and illustrate three new species within Thouarella, from the Weddell Sea, Southern Ocean: T. amundseni sp. nov., T. dolichoespinosa sp. nov. and T. pseudoislai sp. nov. Our species delimitation results suggest, for the first time, the potential presence of Antarctic and sub-Antarctic cryptic species of primnoids, based on the likely presence of sibling species within T. undulata and T. crenelata. With the three new species here described, the global diversity of Thouarella has increased to 41 species, 15 of which are endemic to the Antarctic and sub-Antarctic waters. Consequently, our results provide new steps for uncovering the shelf benthonic macrofauna’s hidden diversity in the Southern Ocean. Finally, we recommend using an integrative taxonomic framework in this group of organisms and species delimitation approaches because the distinctions between some Thouarella species based only on a superficial examination of their macro- and micromorphological features is, in many cases, limited.

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