Combined molecular and morphological phylogeny of Ephemerellinae (Ephemerellidae: Ephemeroptera), with remarks about classification

Zootaxa ◽  
2009 ◽  
Vol 1991 (1) ◽  
pp. 28-42 ◽  
Author(s):  
T. HEATH OGDEN ◽  
JONATHAN T. OSBORNE ◽  
LUKE M. JACOBUS ◽  
MICHAEL F. WHITING
Keyword(s):  
Morphological Analysis ◽  
Sequence Data ◽  
Histone H3 ◽  
Morphological Characters ◽  
Morphological Data ◽  
Rdna Genes ◽  
Dna Sequence Data ◽  
Extant Genus ◽  
Species Groups ◽  
Combined Data

This study represents the first combined molecular and morphological analysis for the mayfly family Ephemerellidae (Ephemeroptera), with a focus on the relationships of genera and species groups of the subfamily Ephemerellinae. The phylogeny was constructed based on DNA sequence data from 3 nuclear (18S rDNA, 28S rDNA, histone H3) and 2 mitochondrial (12S rDNA, 16S rDNA) genes, and 23 morphological characters. Taxon sampling for Ephemerellidae included exemplars from all 25 extant genus groups and additional representatives from those genera with the highest diversity. Ephemerellidae appears to consist of three major clades. Ephemerella, the largest genus of Ephemerellidae, and Serratella were not supported as monophyletic, and each had representatives in two of the three major clades. However, the genera Drunella and Cincticostella were supported as monophyletic. Lineages strongly supported as monophyletic include a grouping of the Timpanoginae genera Timpanoga, Dannella, Dentatella and Eurylophella, and groupings of the Ephemerellinae genera Torleya, Hyrtanella and Crinitella and the genera Kangella, Uracanthella and Teloganopsis. The placement of the Timpanoginae genus Attenella fell within Ephemerellinae, based on molecular and combined data, but it grouped with other Timpanoginae based on morphological data alone. Further study and analysis of Ephemerellidae morphology is needed, and classification should be revised, if it is to reflect phylogenetic relationships.

scholarly journals Synonymization of the male-based ant genus Phaulomyrma (Hymenoptera, Formicidae) with Leptanilla based upon Bayesian total-evidence phylogenetic inference

2020 ◽  
Author(s):  
Zachary H. Griebenow
Keyword(s):  
Sequence Data ◽  
Phylogenetic Inference ◽  
Morphological Characters ◽  
Molecular Data ◽  
Total Evidence ◽  
Morphological Data ◽  
Systematic Revision ◽  
Dna Sequence Data ◽  
Evidence Framework ◽  
Nuclear Loci

Abstract.Although molecular data have proven indispensable in confidently resolving the phylogeny of many clades across the tree of life, these data may be inaccessible for certain taxa. The resolution of taxonomy in the ant subfamily Leptanillinae is made problematic by the absence of DNA sequence data for leptanilline taxa that are known only from male specimens, including the monotypic genus Phaulomyrma Wheeler & Wheeler. Focusing upon the considerable diversity of undescribed male leptanilline morphospecies, the phylogeny of 35 putative morphospecies sampled from across the Leptanillinae, plus an outgroup, is inferred from 11 nuclear loci and 41 discrete male morphological characters using a Bayesian total-evidence framework, with Phaulomyrma represented by morphological data only. Based upon the results of this analysis Phaulomyrma is synonymized with Leptanilla Emery, and male-based diagnoses for Leptanilla that are grounded in phylogeny are provided, under both broad and narrow circumscriptions of that genus. This demonstrates the potential utility of a total-evidence approach in inferring the phylogeny of rare extant taxa for which molecular data are unavailable and begins a long-overdue systematic revision of the Leptanillinae that is focused on male material.

scholarly journals Phylogeny of infraorder Sejina (Acari: Mesostigmata)

Zootaxa ◽  
2004 ◽  
Vol 629 (1) ◽  
pp. 1 ◽  
Author(s):  
MARIAM LEKVEISHVILI ◽  
HANS KLOMPEN
Keyword(s):  
Dna Sequence ◽  
Phylogenetic Relationships ◽  
Sequence Data ◽  
Sister Group ◽  
Morphological Characters ◽  
Morphological Data ◽  
Data Sets ◽  
Dna Sequence Data

Phylogenetic relationships among the families in the infraorder Sejina and the position of Sejina relative to other infraorders of Mesostigmata are re-examined based on molecular and morphological data. Data sets included DNA sequence data for complete 18S, EF-1 , partial CO1genes, and 69 morphological characters. The two families of Heterozerconina consistently group within Sejina, and we propose to synonymize Heterozerconina with Sejina (Sejina s.l). Microgyniina is not the closest relative of Sejina. Rather, Sejina s.l. most often groups with Gamasina. Uropodellidae and Ichthyostomatogasteridae are sister groups and this lineage forms the sister group to Discozerconidae plus Heterozerconidae. Overall, we recognize 5 families within Sejina: Uropodellidae, Ichthyostomatogasteridae, Sejidae, Discozerconidae, and Heterozerconidae.

Molecular phylogeny and systematic evaluation of the Caragana opulens species complex (Fabaceae, Papilionoideae) based on the molecular and morphological data

Phytotaxa ◽  
2021 ◽  
Vol 478 (2) ◽  
pp. 179-200
Author(s):  
SHABIR A. RATHER ◽  
WANG SHU ◽  
MAYANK DHAR DWIVEDI ◽  
CHANG ZHAOYANG
Keyword(s):  
Evolutionary History ◽  
Sequence Data ◽  
Morphological Characters ◽  
Molecular Data ◽  
Morphological Data ◽  
Systematic Evaluation ◽  
Morphological Variations ◽  
Dna Sequence Data ◽  
Different Populations ◽  
Morphological And Molecular Data

In this study, we explored the evolutionary history and taxonomic treatment of the Caragana opulens complex taking information from morphological and molecular data. The complex consists of three species, C. opulens, C. licentiana and C. kansuensis. Moreover, the morphological characters currently used to differentiate the species present in the complex have been found insignificant and inconsistent and do not help diagnose the species. For the present study, we investigated its range and sampled 139 accessions from the different populations of the genus Caragana and 17 accessions of the complex. DNA sequence data from one nrDNA ITS and one cpDNA trnH-psbA loci were sequenced and analyzed using Maximum Likelihood and Bayesian methods. The resulting phylogenies were congruent in topologies. Based on morphological and molecular data, it is concluded that all three species of the complex are one of the same with significant morphological variations. Hence C. opulens is accepted as the correct name along with C. licentiana and C. kansuensis as synonyms.

Assessing the usefulness of histone H3, U2 snRNA and 28S rDNA in analyses of polychaete relationships

1999 ◽  
Vol 47 (5) ◽  
pp. 499 ◽  
Author(s):  
S. Brown ◽  
G. Rouse ◽  
P. Hutchings ◽  
D. Colgan
Keyword(s):  
Maximum Parsimony ◽  
Sequence Data ◽  
Histone H3 ◽  
28S Rdna ◽  
Maximum Parsimony Analysis ◽  
Data Sets ◽  
Data Set ◽  
U2 Snrna ◽  
Dna Sequence Data ◽  
Combined Data

DNA sequence data from for histone H3 (34 species), U2 snRNA (34 species) and two segments (D1 and D9–10 expansion regions) of 28S rDNA (28 and 26 species, respectively) have been collected to investigate the relationships of polychaetes. Representatives of all of the major morphologically identified clades were used, as well as members of the Sipuncula, Echiura, Turbellaria, Clitellata and Siboglinidae (formerly the phyla Pogonophora and Vestimentifera). Maximum parsimony analyses of the separate data sets gave conflicting results and none conformed closely to previous results based on morphology. Instead each data set provided corroboration of a few of the morphological groupings, usually pairing, though inconsistently, members of the same family. Higher groupings proposed on morphological grounds were rarely recovered. Maximum parsimony analysis of the combined data, excluding areas of uncertain alignment, recovered some morphological groupings such as Cirratulidae, Terebellidae, scale worms and eunicimorphs, and did not significantly contradict others. However, some expected groupings were not recovered. Surprisingly, the fanworms (Sabellidae and Serpulidae) were not shown as sister taxa, and monophyly of Phyllodocida, a morphologically well corroborated clade, required four more steps than most parsimonious trees. Aciculata was not seen in our analyses, although it was the most strongly supported large clade in Rouse and Fauchald (1997, Cladistics and polychaetes. Zoologica Scripta 26, 138–204). Trees constrained to show Aciculata as monophyletic were 18 steps longer than the most parsimonious trees. If trees are rooted on sipunculans rather than the nematode, Aciculata is nearly recovered, being rendered paraphyletic by the inclusion of the sister-pair of Oweniidae and Chaetopteridae. As suggested by some recent morphological and molecular analyses, Siboglinidae and Clitellata may well have sister groups among polychaetes. The morphologically aberrant Sternaspidae are closest to members of Terebellida in the present analyses, supporting the placement of Rouse and Fauchald. Interesting results deserving further assessment concern the placement of Chaetopteridae, Oweniidae and Sipuncula.

Synonymisation of the male-based ant genus Phaulomyrma (Hymenoptera:Formicidae) with Leptanilla based upon Bayesian total-evidence phylogenetic inference

2021 ◽  
Author(s):  
Zachary H. Griebenow
Keyword(s):  
Sequence Data ◽  
Phylogenetic Inference ◽  
Morphological Characters ◽  
Molecular Data ◽  
Total Evidence ◽  
Morphological Data ◽  
Systematic Revision ◽  
Dna Sequence Data ◽  
Evidence Framework ◽  
Nuclear Loci

Although molecular data have proven indispensable in confidently resolving the phylogeny of many clades across the tree of life, these data may be inaccessible for certain taxa. The resolution of taxonomy in the ant subfamily Leptanillinae is made problematic by the absence of DNA sequence data for leptanilline taxa that are known only from male specimens, including the monotypic genus Phaulomyrma Wheeler & Wheeler. Focusing upon the considerable diversity of undescribed male leptanilline morphospecies, the phylogeny of 35 putative morphospecies sampled from across the Leptanillinae, plus an outgroup, is inferred from 11 nuclear loci and 41 discrete male morphological characters using a Bayesian total-evidence framework, with Phaulomyrma represented by morphological data only. Based upon the results of this analysis Phaulomyrma is synonymised with Leptanilla Emery, and male-based diagnoses for Leptanilla that are grounded in phylogeny are provided, under both broad and narrow circumscriptions of that genus. This demonstrates the potential utility of a total-evidence approach in inferring the phylogeny of rare extant taxa for which molecular data are unavailable and begins a long-overdue systematic revision of the Leptanillinae that is focused on male material.

Three new species of Diaporthe from China based on morphological characters and DNA sequence data analyses

Phytotaxa ◽  
2019 ◽  
Vol 422 (2) ◽  
pp. 157-174 ◽  
Author(s):  
HAO ZHOU ◽  
CHENG-LIN HOU
Keyword(s):  
Sequence Data ◽  
Histone H3 ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Dna Sequence Data ◽  
Rdna Its ◽  
Three New Species ◽  
Elongation Factor 1

In the present study, nine endophytic strains of Diaporthe from China were identified based on analyses of morphological characters and combined sequences of rDNA ITS, partial sequences from the translation elongation factor 1-α (tef1), β tubulin (tub2), histone H3 (his3), and calmodulin (cal) genes. Three new Diaporthe species, Diaporthe anhuiensis, Diaporthe huangshanensis and Diaporthe shennongjiaensis, are introduced in this paper with full descriptions and comparison with similar taxa. And two other known species, Diaporthe citrichinensis and Diaporthe eres are also described.

A preliminary phylogenetic analysis of the genus Fulvius Stål (Hemiptera:Miridae: Cylapinae) based on molecular data

2008 ◽  
Vol 39 (4) ◽  
pp. 407-417 ◽  
Author(s):  
Izabela Sadowska-Woda ◽  
Tobias Malm ◽  
Frédéric Chérot
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Sequence Data ◽  
Large Subunit ◽  
Morphological Characters ◽  
Molecular Data ◽  
Dna Sequence Data ◽  
Species Groups ◽  
Coi Sequences ◽  
Preliminary Phylogenetic Analysis

AbstractThe systematics and phylogeny of the genus Fulvius Stål remains unclear. We present herein the first analysis of the phylogenetic relationships within the genus Fulvius based on DNA sequences. The phylogenetic interrelationships in the genus Fulvius are investigated using partial DNA sequence data from two mitochondrial genes, the 16S ribosomal large subunit and the cytochrome oxidase I (COI). DNA sequences for Fulvius species representing three different subgroups distinguished previously on the base of morphological characters alone are compared to sequences from the closely related genera Rhinocylapidius and Cylapus. The data are analyzed using parsimony and Bayesian inference. The results confirm that on the basis of molecular data we can distinguish the same congruent groups of Fulvius species as using morphological characters, however with inclusion of the specimens of the genus Rhinocylapidius in the bifenestratus-group. Additional studies are needed to clarify the phylogenetic relationships within the tribe Fulviini, as well as within the genus Fulvius and its relation to Rhinocylapidius. However, the results of this study suggest that 16S and COI sequences will be very useful as molecular markers for such studies among these species-groups.

Genes, morphology and agreement: congruence in Australian anthracine bee flies (Diptera : Bombyliidae : Anthracinae)

2003 ◽  
Vol 17 (2) ◽  
pp. 161 ◽  
Author(s):  
Christine L. Lambkin ◽  
David K. Yeates
Keyword(s):  
Mitochondrial Dna ◽  
Morphological Analysis ◽  
Morphological Characters ◽  
Molecular Data ◽  
Simultaneous Analysis ◽  
Data Sources ◽  
Morphological Data ◽  
Base Pairs ◽  
The World ◽  
Species Groups

Almost half of the 4547 described bee flies (Bombyliidae : Diptera) in the world belong to the subfamily Anthracinae, with most of the world's diversity in three cosmopolitan tribes: Villini, Anthracini and Exoprosopini. Molecular data from 815 base pairs of 16S mitochondrial DNA and morphological characters from species-groups of these tribes in Australia were analysed cladistically. The results show that the relationships between the anthracine tribes reflect those found in a previous morphological analysis. The genera of the Anthracinae in Australia are monophyletic, except for Ligyra Newman, and are assigned to tribes. Although simultaneous analysis of the combined molecular and morphological data produced clades found in both separate analyses, the different data sources are significantly incongruent. We use phylogenetic measures to examine support for the relationships among the Australian Anthracinae inferred by the molecular and morphological data.

Phylogeny and Biogeography of Isoëtes (Isoëtaceae) Based on Nuclear and Chloroplast DNA Sequence Data

2006 ◽  
Vol 31 (3) ◽  
pp. 449-460 ◽  
Author(s):  
Sara B. Hoot ◽  
W. Carl Taylor ◽  
Nancy S. Napier
Keyword(s):  
South African ◽  
New World ◽  
Sequence Data ◽  
Intergenic Spacer ◽  
Morphological Characters ◽  
Dna Sequence Data ◽  
Worldwide Distribution ◽  
The World ◽  
African Clade ◽  
Combined Data

Despite its ancient origins, worldwide distribution, and adaptation to diverse habitats, Isoëtes species have a highly conserved morphology, making it difficult to resolve phylogenetic relationships using morphological characters. In this paper, we report the results from various analyses (maximum parsimony, maximum likelihood, and Bayesian inference) for Isoëtes species from around the world based on nucleotide sequences from the nuclear internal transcribed spacer (ITS) and chloroplast atpB/rbcL intergenic spacer regions. The trees resulting from our analyses of the combined data contain six major well-supported clades (bootstrap ≥ 90%, posterior probabilities 1.00): A clade with possible Gondwanan affinities (I. australis, I. coromandelina, I. panamensis, I. cubana, I. jamaicensis); a South African clade (I. capensis, I. toximontana, I. stellenbossiensis, I. stephansenii); a largely Northern Hemisphere clade (I. nuttallii, I. orcuttii, I. minima, I. dixitei, I. abyssinica, I. olympica, I. longissima, I. velata); an Asian/Australasian clade (I. drummondii, I. gunnii, I. pusilla, I. kirkii, I. muelleri, I. taiwanensis, I. japonica, I. yunguiensis, I. habbemensis); a Mediterranean clade (I. histrix and I. setacea); and a poorly resolved clade consisting of 12 new world species (American species complex). Our results are compared to past classifications and various biogeographical scenarios are explored.

scholarly journals Multiple lines of evidence reveal a new species of Krait (Squamata, Elapidae, Bungarus) from Southwestern China and Northern Myanmar

ZooKeys ◽  
2021 ◽  
Vol 1025 ◽  
pp. 35-71
Author(s):  
Ze-Ning Chen ◽  
Sheng-Chao Shi ◽  
Gernot Vogel ◽  
Li Ding ◽  
Jing-Song Shi
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Morphological Characters ◽  
Morphological Data ◽  
Coi Gene ◽  
Dental Morphology ◽  
Dna Sequence Data ◽  
Coloration Pattern ◽  
Cranial Osteology ◽  
A New Species

Kraits of the genus Bungarus Daudin 1803 are widely known venomous snakes distributed from Iran to China and Indonesia. Here, we use a combination of mitochondrial DNA sequence data and morphological data to describe a new species from Yingjiang County, Yunnan Province, China: Bungarus suzhenaesp. nov. Phylogenetically, this species forms a monophyletic lineage sister to the Bungarus candidus/multicinctus/wanghaotingi complex based on cyt b and ND4 genes but forms a sister species pair with the species B. magnimaculatus Wall & Evans, 1901 based on COI gene fragments. Morphologically, B. suzhenaesp. nov. is similar to the B. candidus/multicinctus/wanghaotingi complex but differs from these taxa by a combination of dental morphology, squamation, coloration pattern, as well as hemipenial morphology. A detailed description of the cranial osteology of the new species is given based on micro-CT tomography images. We revised the morphological characters of B. candidus/multicinctus/wanghaotingi complex and verified the validity of three species in this complex. The distribution of these species was revised; the records of B. candidus in China should be attributed to B. wanghaotingi. We also provide an updated key to species of Bungarus.

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