Three new species of Diaporthe from China based on morphological characters and DNA sequence data analyses

Phytotaxa ◽  
2019 ◽  
Vol 422 (2) ◽  
pp. 157-174 ◽  
Author(s):  
HAO ZHOU ◽  
CHENG-LIN HOU
Keyword(s):  
Sequence Data ◽  
Histone H3 ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Dna Sequence Data ◽  
Rdna Its ◽  
Three New Species ◽  
Elongation Factor 1

In the present study, nine endophytic strains of Diaporthe from China were identified based on analyses of morphological characters and combined sequences of rDNA ITS, partial sequences from the translation elongation factor 1-α (tef1), β tubulin (tub2), histone H3 (his3), and calmodulin (cal) genes. Three new Diaporthe species, Diaporthe anhuiensis, Diaporthe huangshanensis and Diaporthe shennongjiaensis, are introduced in this paper with full descriptions and comparison with similar taxa. And two other known species, Diaporthe citrichinensis and Diaporthe eres are also described.

Phylogeny and morphology reveal two new species of Diaporthe from Betula spp. in China

Phytotaxa ◽  
2016 ◽  
Vol 269 (2) ◽  
pp. 90 ◽  
Author(s):  
ZHUO DU ◽  
XIN-LEI FAN ◽  
KEVIN D. HYDE ◽  
QIN YANG ◽  
YING-MEI LIANG ◽  
...  
Keyword(s):  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Histone H3 ◽  
Elongation Factor ◽  
Betula Platyphylla ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range ◽  
Dieback Disease ◽  
Elongation Factor 1

Diaporthe species are common pathogens, endophytes, or saprobes on a wide range of hosts. During our investigation of forest pathogens, we made collections of Diaporthe species associated with canker and dieback disease of Betula platyphylla and B. albosinensis in Sichuan and Shaanxi provinces in China. Diaporthe betulae sp. nov. and D. betulicola sp. nov. are introduced in this paper, with illustrations, descriptions and support from analysis of ribosomal DNA internal transcribed spacer (ITS), calmodulin (CAL), histone H3 (HIS), translation elongation factor 1-α (TEF1-α) and beta-tubulin (TUB2) sequence data. Diaporthe betulae is characterized by hyaline, ellipsoidal, aseptate, biguttulate, 8.5–11 × 3–4 µm alpha conidia. Diaporthe betulicola is characterized by pycnidial stromata with a single locule with one ostiole per disc. Alpha conidia are hyaline, oblong, aseptate, lack guttules and 9.9–14.7 × 1.3–2.5 µm, and beta conidia are hyaline, spindle-shaped, curved, aseptate and 17–24 × 0.7–1.2 µm.

New species of Echinoderma and Lepiota (Agaricaceae) from China

Phytotaxa ◽  
2020 ◽  
Vol 447 (4) ◽  
pp. 221-236
Author(s):  
YA-JUN HOU ◽  
ZAI-WEI GE
Keyword(s):  
Rna Polymerase Ii ◽  
Large Subunit ◽  
Elongation Factor ◽  
Its Region ◽  
Morphological Characters ◽  
Molecular Evidence ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
The Tropics ◽  
Elongation Factor 1

Three species of Lepiota sensu lato from China are described and illustrated based on morphological characters and molecular evidence. Echinoderma flavidoasperum and Lepiota omninoflava are new to science, while Lepiota echinacea is newly reported from China. Echinoderma flavidoasperum is characterized by a yellow pileus, nearly smooth stipe that discolors light red when bruised, and subcylindrical basidiospores. Lepiota omninoflava, so far only found in the tropics, is characterized by the yellow basidiomata, ellipsoid basidiospores and the absence of cheilocystidia. All three of the Chinese species are discussed and placed within a phylogeny based on the internal transcribed spacer (ITS) region, the large subunit (LSU) of the ribosomal DNA, the translation elongation factor 1-α (tef1-α) and the second largest subunit of RNA polymerase II (rpb2).

scholarly journals Phylogeny, Morphology, Distribution, and Pathogenicity of Botryosphaeriaceae and Diaporthaceae from English Walnut in California

Plant Disease ◽  
2014 ◽  
Vol 98 (5) ◽  
pp. 636-652 ◽  
Author(s):  
ShuaiFei Chen ◽  
David P. Morgan ◽  
Janine K. Hasey ◽  
Kathleen Anderson ◽  
Themis J. Michailides
Keyword(s):  
Sequence Data ◽  
Juglans Regia ◽  
Elongation Factor ◽  
Stem Canker ◽  
Translation Elongation ◽  
Disease Symptoms ◽  
Neoscytalidium Dimidiatum ◽  
Dna Sequence Data ◽  
English Walnut ◽  
Elongation Factor 1

Species of family Botryosphaeriaceae and genus Diaporthe (anamorph: genus Phomopsis, family Diaporthaceae) were reported and caused diseases on various fruit and nut trees in California. In the last several years, diseases on English walnut (Juglans regia) caused by species of Botryosphaeriaceae and Diaporthe were observed frequently in California. Disease symptoms include stem canker; shoot canker and blight; twig, leaf, and fruit blight; and necrotic leaf lesions. Isolates of the pathogen were collected from English walnut in 13 counties in California. The aims of this study were to identify these isolates and to test their pathogenicity to English walnut cultivars. In total, 159 California isolates were identified based on comparisons of DNA sequence data of the internal transcribed spacer, translation elongation factor 1-α, and β-tubulin gene regions, and combined with the morphological features of the cultures and conidia. Research results revealed that isolates represent 10 species of Botryosphaeriaceae and two species of Diaporthe. These species include Botryosphaeria dothidea, Diplodia mutila, D. seriata, Dothiorella iberica, Lasiodiplodia citricola, Neofusicoccum mediterraneum, N. nonquaesitum, N. parvum, N. vitifusiforme, Neoscytalidium dimidiatum, Diaporthe neotheicola, and D. rhusicola. Pathogenicity on three English walnut cultivars (‘Chandler’, ‘Tulare’, and ‘Vina’) using a mycelium plug inoculation method revealed that all these species are pathogenic to all the tested cultivars, with L. citricola and N. parvum being the most pathogenic species, followed by N. mediterraneum, N. dimidiatum, and B. dothidea. Chandler was more tolerant to infection than Tulare and Vina. Results in this study determined that multiple numbers of the Botryosphaeriaceae fungi and two Diaporthe spp. cause cankers and blights of English walnut and vary in their virulence from highly to slightly virulent, respectively.

scholarly journals Identification of the fungal endophyte of Ammophila breviligulata (American beachgrass) as Epichloë amarillans

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4300 ◽  
Author(s):  
Ian Drake ◽  
James F. White Jr ◽  
Faith C. Belanger
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Fungal Endophyte ◽  
Translation Elongation ◽  
Ammophila Breviligulata ◽  
Dna Sequence Data ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

The grass Ammophila breviligulata (American beachgrass) is known to host an endophyte of the genus Epichloë. Based on morphological characteristics it was originally identified as Acremonium typhinum var. ammophilae and is currently designated as Epichloë typhina var. ammophilae. However, the Epichloë species has not previously been identified based on DNA sequence data. Based on phylogenetic placement of beta-tubulin and translation elongation factor 1-alpha DNA sequences the endophyte is identified as a member of E. amarillans rather than E. typhina.

Morphological characters and phylogenetic analysis reveal a new species within the Ganoderma lucidum complex from South Africa

Phytotaxa ◽  
2016 ◽  
Vol 266 (2) ◽  
pp. 115 ◽  
Author(s):  
JIA-HUI XING ◽  
JIE SONG ◽  
CONY DECOCK ◽  
BAO-KAI CUI
Keyword(s):  
South Africa ◽  
New Species ◽  
Ganoderma Lucidum ◽  
Elongation Factor ◽  
Morphological Characters ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Medicinal Properties ◽  
Elongation Factor 1 ◽  
A New Species

Ganoderma is a well-known genus of polypores because of its important medicinal properties. The genus is typified by G. lucidum, which represents a species complex. Ganoderma aridicola sp. nov. is proposed as a new member of the G. lucidum complex from South Africa based on the combination of morphological and molecular evidences. The new species is characterized by its fuscous to black pileal surface with distinct concentric zones and small pores; in addition, it was found in an environment with contrasted dry and humid seasons. Phylogenetically, sequences data from nuclear internal transcribed spacer regions (ITS) and the translation elongation factor 1-α gene (EF1-α) confirmed that G. aridicola is a new species within the G. lucidum complex.

Pleurostoma decline of olive trees caused by Pleurostoma richardsiae in California

Plant Disease ◽  
2020 ◽  
Author(s):  
Daniel P. Lawrence ◽  
Mohamed Taieb Nouri ◽  
Florent P Trouillas
Keyword(s):  
Elongation Factor ◽  
Its Region ◽  
Morphological Characters ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Olive Cultivars ◽  
Nuclear Loci ◽  
Olive Trees ◽  
First Time ◽  
Elongation Factor 1

A single fungal pathogen was consistently isolated from symptomatic wood of olive trees (Olea europaea) displaying branch and trunk cankers in super-high-density orchards in the Sacramento and San Joaquin Valleys of California. Morphological characters of the pathogen included two distinct types of conidia: 1) thick-walled, dark-brown, and globose and 2) thin-walled, hyaline, oblong to ellipsoid and three types of phialides, indicating a pleurostoma-like fungus. Phylogenetic results of four nuclear loci including the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2), and partial sequences of the actin, beta-tubulin, and translation elongation factor 1-α genes confirmed the isolates as Pleurostoma richardsiae. Pathogenicity trials conducted in the field on 2- to 3-year-old branches of three widely planted oil olive cultivars (‘Arbequina’, ‘Arbosana’, and ‘Koroneiki’) satisfied Koch’s postulates and confirmed the pathogenic nature of this species, which is for the first time reported to cause decline of olive trees in California. All three cultivars were equally susceptible to Pl. richardsiae indicating no detectable resistance to the pathogen. Additional isolations from symptomatic hosts including almond, peach, pistachio, and plum also confirmed this species, suggesting that Pl. richardsiae is wide spread in agricultural systems and should be considered an emerging pathogen of fruit and nut crops in California.

New species of Nectria and Thyronectria from Xinjiang, China

Phytotaxa ◽  
2020 ◽  
Vol 433 (4) ◽  
pp. 253-264
Author(s):  
RONG MA ◽  
SHENG-NAN LI ◽  
YING ZHAO ◽  
MIN WANG ◽  
THEMIS J. MICHAILIDES ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Alpha Actin ◽  
First Time ◽  
Elongation Factor 1

Nectria berberidis sp. nov. and Thyronectria berberidicola sp. nov. isolated from Berberis heteropoda in Xinjiang Uygur Autonomous Region, China, are described and illustrated. Nectria berberidis is characterized by clavate asci (50–87 × 8–12 μm) with ellipsoidal to fusiform, 1-septate ascospores. Thyronectria berberidicola is characterized by clavate asci (117–25.9 × 63.7–117.9 μm) with ellipsoidal to fusiform ascospores that have 5–8 transverse septa and 1(–2) longitudinal septum. Ascospores bud to produce hyaline, bacillar ascoconidia. Phylogenetic analyses based on alpha-actin (ACT), the internal transcribed spacer (ITS), the large nuclear ribosomal RNA subunit (LSU), translation elongation factor 1-alpha (TEF1) and the β-tubulin (TUB) sequence data revealed that isolates of N. berberidis and T. berberidicola form a distinct clade within Nectria and Thyronectria, respectively. In addition, Nectria nigrescens is reported for the first time in China.

Seven soil-inhabiting new species of the genus Trichoderma in the Viride clade

Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 28 ◽  
Author(s):  
KAI CHEN ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Culture Characteristics ◽  
Close Relatives ◽  
Encoding Genes ◽  
Elongation Factor 1

Seven new species of the genus Trichoderma belonging to the Viride clade are described from soil samples of different regions in China, and named as T. adaptatum, T. beijingense, T. bifurcatum, T. mangshanicum, T. shaoguanicum, T. tardum and T. vulgatum. Their phylogenetic positions are determined by analyses of the combined partial sequences of translation elongation factor 1-alpha and the second largest RNA polymerase subunit encoding genes. Morphology and culture characteristics are observed, described and illustrated in detail. All the new species form green conidia, fit nicely the Viride clade, but differ obviously from any known species of the genus in sequence data, colony features, growth rates, and size of phialides and conidia. Distinctions between the new species and their close relatives are discussed.

scholarly journals Morphology, Phylogeny and Pathogenicity of Pestalotioid Species on Camellia oleifera in China

2021 ◽  
Vol 7 (12) ◽  
pp. 1080
Author(s):  
Lingling Li ◽  
Qin Yang ◽  
He Li
Keyword(s):  
Woody Plant ◽  
Sequence Data ◽  
Southern China ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Camellia Oleifera ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Elongation Factor 1

Tea-oil tree (Camellia oleifera) is an important edible oil woody plant with a planting area of over 3,800,000 hectares in southern China. Pestalotioid fungi are associated with a wide variety of plants worldwide along with endophytes, pathogens, and saprobes. In this study, symptomatic leaves of C. oleifera were collected from Guangdong, Guangxi, Hainan, Hunan, and Jiangsu Provinces and pestalotioid fungi are characterized based on combined sequence data analyses of internal transcribed spacer (ITS), beta tubulin (tub2), and translation elongation factor 1-alpha (tef-1α) coupled with morphological characteristics. As a result, seven species were confirmed, of which five species are described as new viz. N. camelliae-oleiferae, P. camelliae-oleiferae, P. hunanensis, P. nanjingensis, P. nanningensis, while the other two are reported as known species, viz., N. cubana and N. iberica. Pathogenicity assays showed that all species except for P. nanjingensis developed brown lesions on healthy leaves and P. camelliae-oleiferae showed stronger virulence.

scholarly journals The genus Catathelasma (Catathelasmataceae, Basidiomycota) in China

MycoKeys ◽  
2020 ◽  
Vol 62 ◽  
pp. 123-138
Author(s):  
Zai-Wei Ge ◽  
Jian-Yun Wu ◽  
Yan-Jia Hao ◽  
Qingying Zhang ◽  
Yi-Feng An ◽  
...  
Keyword(s):  
Large Subunit ◽  
Elongation Factor ◽  
Its Region ◽  
Morphological Characters ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Host Preferences ◽  
The North ◽  
Temperate Asia ◽  
Elongation Factor 1

Two new species, Catathelasma laorentou and C. subalpinum, are described on the basis of morphological characters, phylogenetic evidence, host preferences and geographic distributions. A taxonomic key to the known species in China is also provided to facilitate identification. Based on samples from temperate Asia, Europe and North America, the phylogeny of Catathelasma was reconstructed using the internal transcribed spacer (ITS) region, the large subunit (LSU) of the ribosomal DNA and the translation elongation factor 1-α (TEF1).The phylogenetic results showed that Catathelasma contains two monophyletic clades: the /subalpinum clade and the /imperiale clade. The Asian species C. laorentou and C. subalpinum are closely related to the North American C. sp. (labelled as C. ventricosum in GenBank) in the /subalpinum clade, whereas C. imperiale and C. singeri are closely related in the /imperiale clade.

Sign in / Sign up

Export Citation Format

Share Document