The genus Leucophenga (Diptera, Drosophilidae), part VII: the subpollinosa species group from China, with morphological and molecular evidence

Zootaxa ◽  
2017 ◽  
Vol 4247 (3) ◽  
pp. 201 ◽  
Author(s):  
JIA HUANG ◽  
YIRUI SU ◽  
HONGWEI CHEN
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Species Group ◽  
Molecular Evidence ◽  
Neighbor Joining ◽  
Bayesian Analyses ◽  
I Gene

Seventeen species of Leucophenga subpollinosa species group are described from China (including 11 new species): L. argentina (de Meijere, 1924); L. flavicosta Duda, 1926; L. formosa Okada, 1987; L. nigroscutellata Duda, 1924; L. subpollinosa (de Meijere, 1914); L. umbratula Duda, 1924; L. aculeata sp. nov.; L. acuticauda sp. nov.; L. cultella sp. nov.; L. cyclophylla sp. nov.; L. flaviclypeata sp. nov.; L. fuscipedes sp. nov.; L. gracilenta sp. nov.; L. latipenis sp. nov.; L. magnicauda sp. nov.; L. rhombura sp. nov.; L. rugatifolia sp. nov. A key to all examined species of the subpollinosa group in the present study is provided. The phylogenetic relationships among the 17 species of the subpollinosa group are reconstructed by NJ (Neighbor-joining) and Bayesian analyses using 98 DNA sequences of COI (cytochrome c oxidase subunit I) gene. The pairwise intra- and interspecific p-distances of aforementioned sequences are summarized. 

The genus Leucophenga (Diptera, Drosophilidae), part VIII: twenty-one species from the Oriental region, with morphological and molecular evidence

Zootaxa ◽  
2018 ◽  
Vol 4503 (1) ◽  
pp. 1
Author(s):  
JIA HUANG ◽  
YALIAN WANG ◽  
PATRICK MICHAEL O’GRADY ◽  
YIRUI SU ◽  
HONGWEI CHEN
Keyword(s):  
New Species ◽  
Cytochrome C Oxidase ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Species Group ◽  
Molecular Evidence ◽  
Mitochondrial Cytochrome ◽  
Summary Table ◽  
Oriental Region ◽  
Mtcoi Gene

Twenty-one (six known and 15 new) species of the genus Leucophenga from the Oriental region are described or redescribed: L. jacobsoni Duda, 1926; L. kurahashii Okada, 1987; L. setipalpis Duda, 1923; L. sorii Kang, Lee & Bhang, 1965; L. spinifera Okada, 1987; L. varinervis Duda, 1923; L. acantha Huang & Chen, sp. nov.; L. alafumosa Huang & Chen, sp. nov.; L. brevipenis Huang & Chen, sp. nov.; L. brevitabulata Huang & Chen, sp. nov.; L. delta Huang & Chen, sp. nov.; L. forcipula Huang & Chen, sp. nov.; L. fuscipalpula Huang & Chen, sp. nov.; L. glabtabulata Huang & Chen, sp. nov.; L. helvipecta Huang & Chen, sp. nov.; L. hyaloptera Huang & Chen, sp. nov.; L. oxyptera Huang & Chen, sp. nov.; L. platypyga Huang & Chen, sp. nov.; L. serrateiceps Huang & Chen, sp. nov.; L. valvata Huang & Chen, sp. nov.; L. zebrina Huang & Chen, sp. nov. A key and a morphological summary table to all these Leucophenga species are provided. Phylogenetic relationships among these 21 Leucophenga species, another 14 congeneric, known species from seven groups, and two representative outgroup taxa are reconstructed using 169 DNA sequences of the partial mitochondrial cytochrome c oxidase subunit I (mtCOI) gene. In sum, 13 of the 21 Leucophenga species, which possess the only diagnostic character of the proxima species group (abdominal third tergite shortened, anteriorly discolored in males), are not monophyletic. Therefore, all the Leucophenga species described or redescribed in this study are temporarily classified as unplaced species (except for L. sorii, which has been assigned to the sorii species group) to avoid further confusion. 

Morphological and molecular evidence for a new species of the genus Panolis Hübner, [1821] (Lepidoptera: Noctuidae)

Zootaxa ◽  
2019 ◽  
Vol 4565 (4) ◽  
pp. 523
Author(s):  
XUAN AN ◽  
MAMORU OWADA ◽  
MIN WANG ◽  
HOU-SHUAI WANG
Keyword(s):  
New Species ◽  
Cytochrome C Oxidase ◽  
Phylogenetic Analyses ◽  
Species Group ◽  
Molecular Evidence ◽  
Mitochondrial Cytochrome ◽  
Molecular Phylogenetic ◽  
Lepidoptera Noctuidae ◽  
A New Species ◽  
I Gene

A new species of the genus Panolis Hübner, [1821], P. xundian sp. nov., is described and illustrated from southwestern China. It is well-defined morphologically by the male genitalia, with a well-developed pollex at the distal terminal of the sacculus and a broad, ventrally concave cucullus, the female corpus bursae with four long signum-stripes. Based on a 658 bp segment of the mitochondrial cytochrome c oxidase subunit I gene, we report the pairwise genetic distance of 2.5% from its allied species P. exquisita Draudt, 1950. Molecular phylogenetic analyses using three genes (2189 bp in total length) indicate that the new species belongs to the P. exquisita species group. 

Nine new species of the subgenus Stegana (Steganina) from China, with DNA barcoding information (Diptera: Drosophilidae)

Zootaxa ◽  
2019 ◽  
Vol 4576 (1) ◽  
pp. 81
Author(s):  
BINGXUE LI ◽  
YUAN ZHANG ◽  
HONGWEI CHEN
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Molecular Analysis ◽  
Dna Sequences ◽  
Mitochondrial Cytochrome ◽  
Neighbor Joining ◽  
Species Groups ◽  
Mtcoi Gene

Eleven (two known and nine new) species of the subgenus Stegana (Steganina) from China are described or redescribed: S. (S.) longifibula Takada, 1968, S. (S.) toyaensis Okada & Sidorenko, 1992, S. (S.) biflava sp. nov., S. (S.) flavivittata sp. nov., S. (S.) hirtifoliacea sp. nov., S. (S.) latitabula sp. nov., S. (S.) panda sp. nov., S. (S.) pinguifoliacea sp. nov., S. (S.) spatulata sp. nov., S. (S.) stachydifolia sp. nov. and S. (S.) unguiculata sp. nov.; they are assigned into the coleoptrata, ornatipes and undulata species groups, respectively. A total of 130 DNA sequences of partial mitochondrial cytochrome c oxidase subunit I (mtCOI) gene of 38 species (including the 11 species) of above-mentioned three groups are newly obtained in this study. These sequences and other available barcoding sequences of the three groups are involved in a molecular analysis using neighbor-joining (NJ) method, in order to assess the availability of DNA barcoding for delimiting the Steganina species. The result indicates that all the sampled Steganina morphospecies within the three groups are monophyletic.  

scholarly journals Five new pseudocryptic land planarian species of Cratera (Platyhelminthes: Tricladida) unveiled through integrative taxonomy

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9726
Author(s):  
Ana Paula Goulart Araujo ◽  
Fernando Carbayo ◽  
Marta Riutort ◽  
Marta Álvarez-Presas
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Elongation Factor ◽  
Integrative Taxonomy ◽  
Molecular Evidence ◽  
Similar Species ◽  
History Of ◽  
Poorly Differentiated ◽  
I Gene

Background Cratera is a genus of land planarians endemic to the Brazilian Atlantic forest. The species of this genus are distinguished from each other by a series of external and internal characters, nonetheless they represent a challenging taxonomic issue due to the extreme alikeness of the species analysed in the present work. To resolve these difficulties, we have performed morphological analyses and used three nuclear markers (ribosomal 18S and 28S, Elongation Factor, a new anonymous marker named Tnuc813) and two mitochondrial fragments (Cytochrome c oxidase subunit I gene, and a fragment encompasing NADH deshydrogenase subunit 4 gene, trnF and the beginning of the Cytochrome c oxidase I gene) in an integrative taxonomic study. Methods To unveil cryptic species, we applied a molecular species delimitation approach based on molecular discovery methods, followed by a validation method. The putative species so delimited were then validated on the basis of diagnostic morphological features. Results We discovered and described four new species, namely Cratera assu, C. tui, C. boja, and C. imbiri. A fifth new species, C. paraitinga was not highly supported by molecular evidence, but was described because its morphological attributes are unique. Our study documents for the genus Cratera the presence of a number of highly similar species, a situation that is present also in other genera of land planarians. The high number of poorly differentiated and presumably recent speciation events might be explained by the recent geological history of the area.

Taxonomy of the Hirtodrosophila melanderi species group (Diptera: Drosophilidae), with descriptions of four new species from southwestern China

Zootaxa ◽  
2018 ◽  
Vol 4422 (3) ◽  
pp. 345
Author(s):  
TAKEHIRO K. KATOH ◽  
GUANG ZHANG ◽  
CHUAN-JIANG ZHOU ◽  
JIAN-JUN GAO
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Dna Sequences ◽  
New World ◽  
Type Specimen ◽  
Species Group ◽  
Southwestern China ◽  
Mitochondrial Coi ◽  
Respective Type ◽  
I Gene

The Hirtodrosophila melanderi species group contains nine known species recorded from either the Old or the New World. All these species were thought to be strict fungivorous drosophilids. In the present study, we give supplementary descriptions for three of these known species, all recorded from Yunnan, southwestern China, H. furcapenis, H. furcapenisoides, and H. longifurcapenis, by examining respective type specimen(s). We then describe four new species of the same group, H. seticlasper Katoh & Gao, sp. nov., H. spinicerca Katoh & Gao, sp. nov., H. serratifurcapenis Katoh & Gao, sp. nov., and H. truncifurca Katoh & Gao, sp. nov., all discovered recently from high altitudes (ca. 3,500 to 3,800 m a.s.l.) in Tibet (Xizang), southwestern China. The delimitation of these new species is firstly performed in light of morphology and further with the aid of DNA sequences of the mitochondrial COI (cytochrome c oxydase, subunits I) gene. In addition, a key to all the species of the species group is provided. 

Morphological and molecular evidence of some Minuartia species (Caryophyllaceae) from Turkey, with a desciption of a new species

Phytotaxa ◽  
2019 ◽  
Vol 391 (2) ◽  
pp. 122 ◽  
Author(s):  
MURAT KOÇ ◽  
ERGIN HAMZAOĞLU ◽  
AHMET AKSOY
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Related Species ◽  
Phylogenetic Relationships ◽  
Morphological Data ◽  
Molecular Evidence ◽  
A New Species

The genus Minuartia is represented in Turkey by 34 taxa. Some interesting specimens were collected from Antalya province, and examined. These specimens resemble Minuartia meyeri, and M. multinervis from which differ by characters (macro-, and micromorphological) of inflorescence, alar pedicels, petals, sepals, capsules and seeds. Moreover, by using the DNA sequences of the ITS genes, phylogenetic relationships between this collected species, and the related species were investigated. As a result of the evaluation of molecular, and morphological data, we proposed to described the population from Antalya as a new species for the science. A description, pictures, distribution, habitat, and IUCN category are given.

Phylogeny of Dorippoidea (Crustacea : Decapoda : Brachyura) inferred from three mitochondrial genes

2009 ◽  
Vol 23 (3) ◽  
pp. 223 ◽  
Author(s):  
Y. W. Sin ◽  
Joelle C. Y. Lai ◽  
Peter K. L. Ng ◽  
K. H. Chu
Keyword(s):  
Molecular Markers ◽  
Cytochrome C ◽  
16S Rrna ◽  
Cytochrome C Oxidase ◽  
Phylogenetic Relationships ◽  
Phylogenetic Trees ◽  
Mitochondrial Genes ◽  
12S Rrna ◽  
Generic Relationships ◽  
I Gene

The phylogenetic relationships between 10 of 13 genera of crabs from the superfamily Dorippoidea were investigated using mitochondrial 16S rRNA, 12S rRNA and cytochrome c oxidase subunit I gene sequences. The resultant phylogenetic trees based on the three molecular markers support the division of Dorippidae and Ethusidae as monophyletic families within the Dorippoidea. The inferred inter-generic relationships within Dorippidae concur with groupings based on the overall morphology of the carapace and structures of the male first pleopods.

scholarly journals Revision and description of six species of Choeradoplana (Platyhelminthes, Tricladida), with an emendation to the genus

ZooKeys ◽  
2021 ◽  
Vol 1016 ◽  
pp. 1-48
Author(s):  
Domingo Lago-Barcia ◽  
Marcos Santos Silva ◽  
Fernando Carbayo
Keyword(s):  
New Species ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Body Shape ◽  
Oxidase Subunit ◽  
Shape Pattern ◽  
Typical Shape ◽  
A New Species ◽  
I Gene

Living representatives of the Neotropical genus Choeradoplana Graff, 1896 (Geoplaninae, Tricladida, Platyhelminthes) are easily recognized by the typical shape of the head which is laterally expanded, rolled-up, and ventrally provided with two glandular cushions. In this study, the morphology and phylogeny (cytochrome C oxidase subunit I gene) of several species of land planarians are taxonomically investigated. Four of the six species studied are new to science, namely: Ch. eudoxiae Silva & Carbayo, sp. nov., Ch. claudioi Lago-Barcia & Carbayo, sp. nov., Ch. onae Lago-Barcia & Carbayo, sp. nov., and Ch. riutortae Lago-Barcia & Carbayo, sp. nov. The species Choeradoplana albonigra and Ch. eudoxiae deviate from the usual body shape pattern in that the head does not present lateral expansions nor glandular cushions, becoming indistinguishable from its sister genus Cephaloflexa. Pseudogeoplana tristriata (Schultze & Müller, 1857) is also redescribed from a newly collected specimen and was discovered to be a member of Choeradoplana. Graff (1899) also studied another specimen that was considered to be conspecific with P. tristriata; however, in this new it is concluded that it is not conspecific but rather a new species. The name Pseudogeoplana aevipandemiae Lago-Barcia & Carbayo, sp. nov. is suggested for Graff’s specimen.

scholarly journals Coi Gene as a Molecular Marker of Elachista Species (Lepidoptera: Elachistidae: Elachistinae) from Different Lithuanian Populations

2009 ◽  
Vol 63 (1-2) ◽  
pp. 21-24
Author(s):  
Virginijus Sruoga ◽  
Virmantas Stunžėnas ◽  
Brigita Paulavičiūtė
Keyword(s):  
Cytochrome C ◽  
Molecular Marker ◽  
Cytochrome C Oxidase ◽  
Dna Sequences ◽  
Coi Gene ◽  
Oxidase Subunit ◽  
Gene Coi ◽  
I Gene

Coi Gene as a Molecular Marker of Elachista Species (Lepidoptera: Elachistidae: Elachistinae) from Different Lithuanian Populations We compared COI DNA sequences of three Elachista species occurring in Lithuania: Elachista maculicerusella, E. argentella, and E. pollinariella (Gelechioidea: Elachistidae: Elachistinae). Also, intraspecific differences in COI DNA between moth populations were tested. A 705 bp fragment of the 3'-end of cytochrome c oxidase subunit I gene (COI) was used. This mtDNA fragment was significantly different between all studied species. Intraspecific differences were detected only for E. maculicerusella from different Lithuanian populations. Our results support using the COI gene for identification of Elachista species and as a tool for exploring intraspecific differences between populations.

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