Nine new species of the subgenus Stegana (Steganina) from China, with DNA barcoding information (Diptera: Drosophilidae)

Zootaxa ◽  
2019 ◽  
Vol 4576 (1) ◽  
pp. 81
Author(s):  
BINGXUE LI ◽  
YUAN ZHANG ◽  
HONGWEI CHEN

Eleven (two known and nine new) species of the subgenus Stegana (Steganina) from China are described or redescribed: S. (S.) longifibula Takada, 1968, S. (S.) toyaensis Okada & Sidorenko, 1992, S. (S.) biflava sp. nov., S. (S.) flavivittata sp. nov., S. (S.) hirtifoliacea sp. nov., S. (S.) latitabula sp. nov., S. (S.) panda sp. nov., S. (S.) pinguifoliacea sp. nov., S. (S.) spatulata sp. nov., S. (S.) stachydifolia sp. nov. and S. (S.) unguiculata sp. nov.; they are assigned into the coleoptrata, ornatipes and undulata species groups, respectively. A total of 130 DNA sequences of partial mitochondrial cytochrome c oxidase subunit I (mtCOI) gene of 38 species (including the 11 species) of above-mentioned three groups are newly obtained in this study. These sequences and other available barcoding sequences of the three groups are involved in a molecular analysis using neighbor-joining (NJ) method, in order to assess the availability of DNA barcoding for delimiting the Steganina species. The result indicates that all the sampled Steganina morphospecies within the three groups are monophyletic.  

Zootaxa ◽  
2019 ◽  
Vol 4657 (3) ◽  
pp. 503-522
Author(s):  
MARIA VALLADOLID ◽  
IOANNIS KARAOUZAS ◽  
MERCEDES ARAUZO ◽  
BEATRIZ A. DORDA ◽  
ISABEL REY

The morphology of the different stages (larva, pupa, male, and female) of Rhyacophila fasciata kykladica Malicky & Sipahiler 1993 was examined as a basis for the description of the taxon. Morphological data were supplemented by molecular analysis of mitochondrial cytochrome C oxidase subunit I (mtCOI), which were compared with samples of the nominate subspecies Rhyacophila fasciata fasciata Hagen 1859, as well as with other new species in this group, Rhyacophila denticulata McLachlan 1879 and Rhyacophila sociata Navás 1916. Our results revealed genetic differences between all the taxa; therefore, a change of taxonomic status of R. fasciata kykladica to R. kykladica (stat. prom.) is proposed.


Zootaxa ◽  
2017 ◽  
Vol 4247 (3) ◽  
pp. 201 ◽  
Author(s):  
JIA HUANG ◽  
YIRUI SU ◽  
HONGWEI CHEN

Seventeen species of Leucophenga subpollinosa species group are described from China (including 11 new species): L. argentina (de Meijere, 1924); L. flavicosta Duda, 1926; L. formosa Okada, 1987; L. nigroscutellata Duda, 1924; L. subpollinosa (de Meijere, 1914); L. umbratula Duda, 1924; L. aculeata sp. nov.; L. acuticauda sp. nov.; L. cultella sp. nov.; L. cyclophylla sp. nov.; L. flaviclypeata sp. nov.; L. fuscipedes sp. nov.; L. gracilenta sp. nov.; L. latipenis sp. nov.; L. magnicauda sp. nov.; L. rhombura sp. nov.; L. rugatifolia sp. nov. A key to all examined species of the subpollinosa group in the present study is provided. The phylogenetic relationships among the 17 species of the subpollinosa group are reconstructed by NJ (Neighbor-joining) and Bayesian analyses using 98 DNA sequences of COI (cytochrome c oxidase subunit I) gene. The pairwise intra- and interspecific p-distances of aforementioned sequences are summarized. 


Zootaxa ◽  
2018 ◽  
Vol 4503 (1) ◽  
pp. 1
Author(s):  
JIA HUANG ◽  
YALIAN WANG ◽  
PATRICK MICHAEL O’GRADY ◽  
YIRUI SU ◽  
HONGWEI CHEN

Twenty-one (six known and 15 new) species of the genus Leucophenga from the Oriental region are described or redescribed: L. jacobsoni Duda, 1926; L. kurahashii Okada, 1987; L. setipalpis Duda, 1923; L. sorii Kang, Lee & Bhang, 1965; L. spinifera Okada, 1987; L. varinervis Duda, 1923; L. acantha Huang & Chen, sp. nov.; L. alafumosa Huang & Chen, sp. nov.; L. brevipenis Huang & Chen, sp. nov.; L. brevitabulata Huang & Chen, sp. nov.; L. delta Huang & Chen, sp. nov.; L. forcipula Huang & Chen, sp. nov.; L. fuscipalpula Huang & Chen, sp. nov.; L. glabtabulata Huang & Chen, sp. nov.; L. helvipecta Huang & Chen, sp. nov.; L. hyaloptera Huang & Chen, sp. nov.; L. oxyptera Huang & Chen, sp. nov.; L. platypyga Huang & Chen, sp. nov.; L. serrateiceps Huang & Chen, sp. nov.; L. valvata Huang & Chen, sp. nov.; L. zebrina Huang & Chen, sp. nov. A key and a morphological summary table to all these Leucophenga species are provided. Phylogenetic relationships among these 21 Leucophenga species, another 14 congeneric, known species from seven groups, and two representative outgroup taxa are reconstructed using 169 DNA sequences of the partial mitochondrial cytochrome c oxidase subunit I (mtCOI) gene. In sum, 13 of the 21 Leucophenga species, which possess the only diagnostic character of the proxima species group (abdominal third tergite shortened, anteriorly discolored in males), are not monophyletic. Therefore, all the Leucophenga species described or redescribed in this study are temporarily classified as unplaced species (except for L. sorii, which has been assigned to the sorii species group) to avoid further confusion. 


ZooKeys ◽  
2020 ◽  
Vol 960 ◽  
pp. 1-15
Author(s):  
Chi-Woo Lee ◽  
Ko Tomikawa ◽  
Gi-Sik Min

A new species of pseudocrangonyctid amphipod, Pseudocrangonyx wonkimisp. nov., was found in the groundwater of a cave in the southwestern Korean Peninsula. Pseudocrangonyx wonkimisp. nov. is morphologically most closely related to P. joolaeiLee et al., 2020. However, P. wonkimi is clearly distinguished from P. joolaei by lacking sternal gills, fewer setae on maxilla 1 inner plate, fewer serrate robust setae on the carpus of the gnathopods, lacking bifid setae on the inner ramus of pleopod 3, and fewer articles of rami on pleopod 3. We also determined sequences of mitochondrial cytochrome c oxidase subunit I (COI) of P. wonkimisp. nov. for molecular diagnosis. From the molecular analysis based on COI sequences, P. wonkimi showed the closest relationship with P. joolaei with 15.1% genetic distance.


Zootaxa ◽  
2020 ◽  
Vol 4750 (2) ◽  
pp. 182-190
Author(s):  
MASAKUNI OKAZAKI ◽  
SUSUMU OHTSUKA ◽  
KO TOMIKAWA

A new species of the eusirid amphipod, Rhachotropis reiwa is described from off Amamioshima Island, northwestern Pacific. The new species differs from its congeners in having large eyes, the middorsal tooth on pereonite 7, pleonite 3 and urosomite 1 without middorsal and dorsolateral teeth, the basis of pereopod 5 strongly produced posteriorly, and pereopod 6 with the triangular basis. A key to the species of Rhachotropis from Japanese and adjacent waters is provided. Additionally, a nucleotide sequence of mitochondrial cytochrome c oxidase subunit I from its holotype was determined for the future study. 


2019 ◽  
Vol 24 (11) ◽  
pp. 2167-2183
Author(s):  
Yeong-deok Han ◽  
Sergey V. Mironov ◽  
Gi-sik Min

Two new species of feather mites from the superfamily Analgoidea are described from the grey-headed woodpecker, Picus canus, in Korea: Neopteronyssus koreanus sp. nov. (Pteronyssidae) and Proterothrix picinus sp. nov. (Proctophyllodidae: Pterodectinae). Feather mites of the genera Neopteronyssus Mironov, 2002 and Proterothrix Gaud, 1968 are described for the first time in Korea. Morphological descriptions of both new species are complemented with partial sequences of their mitochondrial cytochrome c oxidase subunit I (COI) gene as DNA barcodes.


Zootaxa ◽  
2020 ◽  
Vol 4852 (2) ◽  
pp. 151-165
Author(s):  
MICHAEL BALKE ◽  
YOANDRI S. MEGNA ◽  
NILVER ZENTENO ◽  
LUIS FIGUEROA ◽  
LARS HENDRICH

We describe four new species of the diving beetle genus Liodessus Guignot from the high Andean regions of Peru: Liodessus alpinus sp. nov. from Junín, L. hauthi sp. nov. from Huánuco and Churubamba, L. rhigos sp. nov. from Junín, and L. thespesios sp. nov. from Cusco. We delineate the species using morphological structures and provide a 5’ mitochondrial cytochrome c oxidase 1 database on the Barcode of Life Data System (BOLD). We also provide taxonomic notes on Liodessus acollensis Guignot, 1955 and L. andinus Guignot, 1957, described from the high Andes of Peru and Bolivia. These species occur at higher altitudes above 3,400 m and up to 4,900 m, and were collected in shallow, exposed peatland pools and puddles, mostly in steppes and high Andean Puna. The known distribution and habitat preferences of each species are outlined briefly. 


2021 ◽  
Vol 86 ◽  
pp. 63-77
Author(s):  
Sangjin Kim ◽  
Željko Tomanović ◽  
Yeonghyeok Yu ◽  
JuHyeong Sohn ◽  
Yunjong Han ◽  
...  

Several species of the genus Aphidius are well known as commercial biocontrol agents of pest aphids, and more than 130 species of the genus have been recorded worldwide. To date, only 15 Aphidius species have been recorded in South Korea. Using the DNA barcode region (ca. 658 bp) of mitochondrial cytochrome c oxidase subunit I (COI), we amplified sequences of 15 Aphidius Korean species, aligned them in combination with 23 reference species retrieved from GenBank for comparison and identification, and then reconstructed a barcode phylogeny by the neighbour-joining method. As a result, three Aphidius species were found to be new to Science. Descriptions and illustrations of the three species new to Science – Aphidius longicarpussp. nov., A. longistigmussp. nov., and A. asiaticussp. nov. – are provided, together with their phylogenetic position within the genus Aphidius. In addition, a redescription of A. areolatus, a parasitoid of maple aphids (Peryphillus spp.), is also given.


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