Morphological and molecular characterization of three Helicotylenchus species from Iran

Zootaxa ◽  
2018 ◽  
Vol 4388 (2) ◽  
pp. 207 ◽  
Author(s):  
EBRAHIM SHOKOOHI ◽  
MARIETTE MARAIS ◽  
HENDRIKA FOURIE ◽  
HADI PANAHI
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
28S Rdna ◽  
Molecular Characteristics ◽  
Monophyletic Clade ◽  
Morphological And Molecular Characterization

Three Helicotylenchus species, namely Helicotylenchus digonicus, Helicotylenchus pseudorobustus and Helicotylenchus vulgaris were identified during a nematode survey conducted in Iran. The morphological and molecular characteristics of the species were defined. In addition, phylogenetic analysis of five Iranian Helicotylenchus populations based on 28S rDNA were done and showed the unresolved position of molecularly identified H. pseudorobustus and H. digonicus. However, populations of H. vulgaris and H. digonicus were placed close to each other. Phylogenetic analysis confirmed that molecularly characterized Helicotylenchus species formed a monophyletic clade. A detailed revision of the species is, however, needed. Illustrations and measurements for all species are provided. 

Morphological and molecular characterization of Buzionema lutgardae n. sp. (Nematoda: Oxyuridomorpha: Thelastomatidae) from the cockroach Byrsotria sp. (Blattaria: Blaberidae) in Cuba

Zootaxa ◽  
2021 ◽  
Vol 4965 (2) ◽  
pp. 385-395
Author(s):  
JANS MORFFE ◽  
NAYLA GARCÍA ◽  
KOICHI HASEGAWA ◽  
RAMON A. CARRENO
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Sister Group ◽  
Cylindrical Part ◽  
28S Rdna ◽  
Monophyletic Clade ◽  
Basal Bulb ◽  
Morphological And Molecular Characterization

Buzionema lutgardae n. sp. (Nematoda: Oxyuridomorpha: Thelastomatidae) is described from the cockroach Byrsotria sp. (Blattaria: Blaberidae), endemic to Cuba. Females of B. lutgardae n. sp. are shorter than those of B. validum Kloss, 1966 (1600–2150 µm vs. 3131–3378 µm), but the oesophagus is comparatively longer (b = 2.96–3.77 vs. 4.65–4.87). The lateral alae of the new species extend from ca. the midpoint of the cylindrical part of the procorpus to the level of the anus in contrast to the base of the basal bulb to the level of the anus in B. validum. The males of B. lutgardae n. sp. are shorter than those of B. validum (780–940 µm vs. 1177–1423 µm) and their lateral alae end at some distance before the cloaca instead the level of the cloaca in B. validum. The phylogeny of B. lutgardae n. sp. is inferred by the D2-D3 domains of the 28S rDNA. B. lutgardae n. sp. and B. validum form a monophyletic clade with strong nodal support, as sister-group of the genus Leidynema Schwenck in Travassos, 1929. 

Morphological and molecular characterization of Paractinolaimus sahandi n. sp. (Nematoda: Actinolaimidae) from the Sahand Mountains in Iran

2010 ◽  
Vol 85 (3) ◽  
pp. 276-282 ◽  
Author(s):  
M. Pedram ◽  
G. Niknam ◽  
M.T. Vinciguerra ◽  
W. Ye ◽  
R.T. Robbins
Keyword(s):  
New Species ◽  
Molecular Characterization ◽  
Outer Layer ◽  
18S Rdna ◽  
Soil Samples ◽  
28S Rdna ◽  
First Report ◽  
Morphological And Molecular Characterization

AbstractParactinolaimus sahandi n. sp., found in wet soil samples collected from the rhizosphere of grasses of Sahand Mountains, Iran, is described. This new species is characterized by its long body (3.5–4.7 mm), high a value (74.5–88.5), anterior location of posterior subventral nuclei, occupying 62.5–68.0% of glandularium distance, the presence of 1–4 pre- and 1–3 post-vulval papillae and numerous tiny, not innervated papillae in front and behind the vulva in the outer layer of cuticle; common functional males in the population, with 62.5–81.3 μm long spicules and 15–17 ventromedian supplements. The new species, which is the only one in the genus showing the advulval cuticular tiny papillae and is unusually slender, is compared to four species of Paractinolaimus, namely P. macrolaimus, P. longidrilus, P. spanithelus and P. rafiqi. The ribosomal 18S rDNA (1246 bp sequenced) and 28S rDNA D2/D3 region (844 bp sequenced) of P. sahandi n. sp. were sequenced for molecular characterization. Sequences of the 18S and 28S D2/D3 of P. sahandi n. sp. have distinct differences from those of the only sequenced P. macrolaimus, with 6 bp differences in 18S and 38 bp differences and five gaps in 28S. This is the first report of the occurrence of members of Actinolaimidae in Iran.

Morphological and molecular characterization of Lepidonema magnum Morffe & García, 2010 (Nematoda: Oxyuridomorpha: Hystrignathidae) from Passalus interstitialis Eschscholtz, 1829 (Coleoptera: Passalidae) from Cuba and new locality records for the species

Zootaxa ◽  
2019 ◽  
Vol 4551 (2) ◽  
pp. 221
Author(s):  
JANS MORFFE ◽  
NAYLA GARCÍA ◽  
KARIN BREUGELMANS ◽  
KOICHI HASEGAWA ◽  
ANDREW K. DAVIS
Keyword(s):  
Molecular Characterization ◽  
Genital Tract ◽  
Ssu Rdna ◽  
Phylogenetic Position ◽  
Lsu Rdna ◽  
Monophyletic Clade ◽  
Morphological And Molecular Characterization

Lepidonema magnum Morffe & García, 2010 (Nematoda: Oxyuridomorpha: Hystrignathidae) is redescribed and illustrated with the aid of SEM. New features of the cephalic end and genital tract of the females were observed. New locality records are given. The phylogenetic position of the species is inferred on the basis of the D2-D3 segment of the 28S LSU rDNA and 18S SSU rDNA. L. magnum forms a monophyletic clade formed by other hystrignathids: Coynema poeyi (Coy, García & Álvarez, 1993), two species of Longior Travassos & Kloss, 1958 and two Hystrignathus Leidy, 1850. 

scholarly journals Morphological and Molecular Characterization of Alternaria sect. Ulocladioides/Ulocladium Isolated from Citrus Fruits in Upper Egypt

2020 ◽  
pp. 30-41
Author(s):  
Youssuf A. Gherbawy ◽  
Thanaa A. Maghraby ◽  
Karima E. Abdel Fattah ◽  
Mohamed A. Hussein
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Navel Orange ◽  
Citrus Fruits ◽  
Upper Egypt ◽  
Important Crop ◽  
Alternaria Species ◽  
Morphological And Molecular Characterization ◽  
Toxin Producers

Citrus is the most important crop in Upper Egypt. 150 infected samples were collected from citrus samples (Navel orange, Tangerine and Lemon) in Upper Egypt, 50 samples from each fruit. Twenty-two isolates representing three species of Alternaria belong to A. sect. Ulocladioides and A. sect. Ulocladium were isolated on dichloran chloramphenicol- peptone agar (DCPA) medium at 27°C. Tangerine samples were more contaminated with Alternaria followed by Navel orange and no Alternaria species appeared from Lemon samples. Based on the Alt a1 the phylogenetic analysis identified the isolates as Alternaria atra, Alternaria botrytis and Alternaria oudemansii. The pathogenicity of the isolates was tested by inoculation of healthy navel orange, the resulted data showed that all tested isolates were pathogenic to healthy navel orange with different degrees ranged from 31.5±1 - 20±1 mm and A. oudemansii had a low virulent effect. The mycotoxins ability of tested isolates indicated that about 83% of the isolates were TeA toxin producers with amount ranged from 1.54 - 18.47 ug/ml.

Morphological and molecular characterization of Coynema poeyi (Coy, García & Álvarez, 1993) (Oxyuridomorpha: Hystrignathidae) from Antillanax pertyi (Kaup, 1869) (Coleoptera: Passalidae) from Cuba and new locality records for the species

Zootaxa ◽  
2018 ◽  
Vol 4497 (1) ◽  
pp. 29
Author(s):  
JANS MORFFE ◽  
NAYLA GARCÍA ◽  
KARIN BREUGELMANS ◽  
BYRON J. ADAMS ◽  
KOICHI HASEGAWA
Keyword(s):  
Molecular Characterization ◽  
Phylogenetic Position ◽  
Lsu Rdna ◽  
Generic Diagnosis ◽  
Monophyletic Clade ◽  
Morphological And Molecular Characterization

Coynema poeyi (Coy, García & Álvarez, 1993) (Oxyuridomorpha: Hystrignathidae) is redescribed and illustrated with the aid of SEM. New features of the cephalic end of both sexes and copulatory papillae pattern of the males were observed and the generic diagnosis is emended in order to include such features. New locality records are given. The phylogenetic position of the species is inferred on the basis of the D2-D3 segment of the LSU rDNA. C. poeyi is located basal in a monophyletic clade formed by other hystrignathids: two species of Longior Travassos & Kloss, 1958 and Hystrignathus sp. 

scholarly journals Morphological and molecular characterization of Termitomyces (Lyophyllaceae, Agaricales) in Thailand

2020 ◽  
Vol 21 (6) ◽  
Author(s):  
NICHAREE JANNUAL ◽  
MINGKWAN NIPITWATTANAPHON ◽  
SASITORN HASIN ◽  
Tharnrat Kaewgrajang
Keyword(s):  
Molecular Characterization ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Morphological Data ◽  
Molecular Characteristics ◽  
Its2 Region ◽  
Diagnostic Key ◽  
Morphological And Molecular Characterization ◽  
Home Consumption

Abstract. Jannual N, Nipitwattanaphon M, Hasin S, Kaewgrajang T. 2020. Morphological and molecular characterization of Termitomyces (Lyophyllaceae, Agaricales) in Thailand. Biodiversitas 21: 2481-2491. Termitomyces is considered to be a highly prized delicacy collected both for home consumption and for sale in local markets. Although the taxonomic information about this genus is well known in Africa, the identification of Termitomyces species in Thailand is unclear. Therefore, this study presented an assessment of phylogenetic relationships in the genus Termitomyces by means of sequencing of the ITS1-5.8S-ITS2 region and the species characterization by combination of morphological data and molecular data. Total of 61 Termitomyces specimens was collected from a variety of geographical localities of Thailand. They were classified into six species, including T. clypeatus, T. cylindricus, T. fuliginosus, T. heimii, T. microcarpus, and T. striatus based on morphological characteristics combining with molecular characteristics. This is the first diagnostic key of Thai Termitomyces.

Morphological, histological and molecular characterization of Myxidium cf. rhodei infecting the kidney of Rutilus rutilus

2020 ◽  
Vol 141 ◽  
pp. 39-46
Author(s):  
MD Dorjievna Batueva ◽  
X Pan ◽  
J Zhang ◽  
X Liu ◽  
W Wei ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Rutilus Rutilus ◽  
Longitudinal Axis ◽  
Suture Line ◽  
Present Species ◽  
Supplementary Data

In the present study, we provide supplementary data for Myxidium cf. rhodei Léger, 1905 based on morphological, histological and molecular characterization. M. cf. rhodei was observed in the kidneys of 918 out of 942 (97%) roach Rutilus rutilus (Linnaeus, 1758). Myxospores of M. cf. rhodei were fusiform with pointed ends, measuring 12.7 ± 0.1 SD (11.8-13.4) µm in length and 4.6 ± 0.1 (3.8-5.4) µm in width. Two similar pear-shaped polar capsules were positioned at either ends of the longitudinal axis of the myxospore: each of these capsules measured 4.0 ± 0.1 (3.1-4.7) µm in length and 2.8 ± 0.1 (2.0-4.0) µm in width. Polar filaments were coiled into 4 to 5 turns. Approximately 18-20 longitudinal straight ridges were observed on the myxospore surface. The suture line was straight and distinctive, running near the middle of the valves. Histologically, the plasmodia of the present species were found in the Bowman’s capsules, and rarely in the interstitium of the host. Phylogenetic analysis revealed that M. cf. rhodei was sister to M. anatidum in the Myxidium clade including most Myxidium species from freshwater hosts.

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