Morphological and molecular characterization of Termitomyces (Lyophyllaceae, Agaricales) in Thailand

Abstract. Jannual N, Nipitwattanaphon M, Hasin S, Kaewgrajang T. 2020. Morphological and molecular characterization of Termitomyces (Lyophyllaceae, Agaricales) in Thailand. Biodiversitas 21: 2481-2491. Termitomyces is considered to be a highly prized delicacy collected both for home consumption and for sale in local markets. Although the taxonomic information about this genus is well known in Africa, the identification of Termitomyces species in Thailand is unclear. Therefore, this study presented an assessment of phylogenetic relationships in the genus Termitomyces by means of sequencing of the ITS1-5.8S-ITS2 region and the species characterization by combination of morphological data and molecular data. Total of 61 Termitomyces specimens was collected from a variety of geographical localities of Thailand. They were classified into six species, including T. clypeatus, T. cylindricus, T. fuliginosus, T. heimii, T. microcarpus, and T. striatus based on morphological characteristics combining with molecular characteristics. This is the first diagnostic key of Thai Termitomyces.

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Morphological and molecular characterization of Posthodiplostomum sp. (Digenea: Diplostomidae) metacercaria in the muscles of snakeheads (Channa punctata) from Manipur, India

Helminthologia ◽

10.2478/s11687-014-0221-z ◽

2014 ◽

Vol 51 (2) ◽

pp. 141-152 ◽

Cited By ~ 7

Author(s):

V. Athokpam ◽

V. Tandon

Keyword(s):

Molecular Characterization ◽

Sequence Similarity ◽

Northeast India ◽

Fish Host ◽

Its2 Region ◽

Morphological Identification ◽

Channa Punctata ◽

Rna Genes ◽

Morphological And Molecular Characterization

Abstract The spotted snakehead, Channa punctata Bloch, 1793, is a locally important fish species commonly consumed by the natives in the state of Manipur, Northeast India. The fish host C. punctata from Lamphel area revealed a diplostomid metacercarial infection. Morphologically, the recovered metacercaria was identified as a species of Posthodiplostomum Dubois, 1936. Molecular characterization using the ribosomal RNA genes (rDNA 18S, ITS2 and 28S regions) and the mitochondrial CO1 region supplements the identification. Molecular analysis revealed the metacercaria to be closely related to Posthodiplostomum sp. Japan isolate, with sequence similarity variation from 97.5–99.7 % while considering for the three rDNA markers. The secondary structure of the ITS2 region further corroborated these results; the typical four-helix model, when compared to the taxon from Japan, showed differences only in twelve bases (with seven transitions and five transversions). In phylogenetic analysis also, the metacercaria claded with the genus Posthodiplostomum, coming closer to the Japanese isolate, thus supplementing the morphological identification of the metacercaria.

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Characterization of Three Species of Thrips on Weeping Fig, Nutmeg, and Marine Seruni Plants Based on Mtcoi DNA Sequences

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v8i2.5448 ◽

2016 ◽

Vol 8 (2) ◽

pp. 185

Author(s):

Nia Kurniawaty ◽

Purnama Hidayat ◽

Aunu Rauf

Keyword(s):

Pest Management ◽

Molecular Characterization ◽

Dna Sequences ◽

Biology Education ◽

Morphological Data ◽

Coi Gene ◽

Molecular Characteristics ◽

Vegetable Crops ◽

Total Extraction

Thrips are widely reported as pests in vegetable crops. However, the existence of Phlaeothripidae members has a less concern in Indonesia. Phlaeothripidae is the only family of Tubulifera Suborder and some reports suggested that they had potential to be pests in several crops due to their ability to roll up and to make galls on leaves. The first step in pest management attempt is to identify the pest accurately and quickly, so the pest management can be on target and more efficient. One of the identification methods is the molecular identification using DNA barcoding techniques. This study aimed to characterize and to compare species thrips in banyan, nutmeg, and marine seruni based on their molecular characteristics. This research was conducted in Bogor and Kuningan. The process of molecular characterization consisteds of four steps DNA total extraction, amplification by using PCR, COI gene sequence, and data analysis. PCR programme was succesfully to amplified mtCOI gene fragment at 710 bp. The length of mtCOI gene of Gynaikothrips uzeli, Haplothrips ganglbaueri, and Pseudophilothrips ichini were 704, 686, and 702 bp dominated by A and T bases with nucleotide variation value of 27.8%. This results confirmed that molecular characterization using mtCOI gene mitochondrial had successfully supported the morphological data. How to CiteKurniawaty, N., Hidayat, P. & Rauf, A. (2016). Characterization of Three Species of Thrips on Banyan, Nutmeg, and Marine Seruni Plants Based on Coi Gene. Biosaintifika: Journal of Biology & Biology Education, 8(2), 185-192.

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Morphological and molecular characterization of three Helicotylenchus species from Iran

Zootaxa ◽

10.11646/zootaxa.4388.2.4 ◽

2018 ◽

Vol 4388 (2) ◽

pp. 207 ◽

Cited By ~ 6

Author(s):

EBRAHIM SHOKOOHI ◽

MARIETTE MARAIS ◽

HENDRIKA FOURIE ◽

HADI PANAHI

Keyword(s):

Phylogenetic Analysis ◽

Molecular Characterization ◽

28S Rdna ◽

Molecular Characteristics ◽

Monophyletic Clade ◽

Morphological And Molecular Characterization

Three Helicotylenchus species, namely Helicotylenchus digonicus, Helicotylenchus pseudorobustus and Helicotylenchus vulgaris were identified during a nematode survey conducted in Iran. The morphological and molecular characteristics of the species were defined. In addition, phylogenetic analysis of five Iranian Helicotylenchus populations based on 28S rDNA were done and showed the unresolved position of molecularly identified H. pseudorobustus and H. digonicus. However, populations of H. vulgaris and H. digonicus were placed close to each other. Phylogenetic analysis confirmed that molecularly characterized Helicotylenchus species formed a monophyletic clade. A detailed revision of the species is, however, needed. Illustrations and measurements for all species are provided.

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Morphological and molecular characterization of three new Parastenocarididae (Copepoda: Harpacticoida) from caves in Southern Italy

European Journal of Taxonomy ◽

10.5852/ejt.2020.689 ◽

2020 ◽

Author(s):

Maria Cristina Bruno ◽

Vezio Cottarelli ◽

Federico Marrone ◽

Rosario Grasso ◽

Erika Stefani ◽

...

Keyword(s):

Southern Italy ◽

Molecular Study ◽

Molecular Data ◽

Morphological Data ◽

Ecological Knowledge ◽

Mitochondrial Coi ◽

Copepodid Stage ◽

Morphological And Molecular Data ◽

Morphological And Molecular Characterization

We describe three new parastenocaridid: Cottarellicaris sanctiangeli Bruno & Cottarelli sp. nov., Stammericaris vincentimariae Bruno & Cottarelli sp. nov. and Proserpinicars specincola Bruno & Cottarelli sp. nov., collected in the pools of five different caves located in Calabria (Southern Italy). We conducted a phylogenetic analysis based on the mitochondrial COI and ribosomal 18S sequences of C. sanctiangeli sp. nov. and S. vincentimariae sp. nov., and of four more species of Stammericaris and one Proserpinicaris available from literature. Based on the molecular study, the specimens of C. sanctiangeli sp. nov. are clearly separated from the species belonging to the closely-related genus Stammericaris. The morphological and molecular data indicate that the genera belonging to the two subfamilies Parastenocaridinae and Fontinalicaridinae form two monophyletic and distinct clades, thus supporting their status. We also provide insights on the genus Proserpinicaris based on morphological data only; in particular, the most important synapomorphic character of the genus, i.e., the taxonomic value of the hyaline structure inserted on the anterior surface of the male leg 4 basis, is discussed based on the development of the P4 endopod, as observed in the last copepodid stage of some species of this genus. Finally, we widen the biogeographic and ecological knowledge of the three genera.

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Morphological and Molecular Characterization of Brasilonema roberti-lamii (Cyanophyceae, Nostocales, Scytonemataceae), from Central Mexico

Phytotaxa ◽

10.11646/phytotaxa.164.4.4 ◽

2014 ◽

Vol 164 (4) ◽

pp. 255 ◽

Cited By ~ 8

Author(s):

BEATRIZ RODARTE ◽

ITZEL BECERRA-ABSALÓN ◽

GUSTAVO A. MONTEJANO ◽

KARINA OSORIO-SANTOS ◽

LUISA ALBA-LOIS ◽

...

Keyword(s):

Molecular Characterization ◽

Growth Form ◽

Good Alternative ◽

Molecular Data ◽

Central Mexico ◽

Rrna Gene ◽

Morphological Criteria ◽

Ecological Criteria ◽

Morphological And Molecular Characterization

This paper is a contribution to the morphological and molecular characterization of the cyanobacterium Brasilonema roberti-lamii from populations found in Central Mexico. The general growth form and the morphological, morphometric and ecological characteristics of the populations studied clearly correspond to those described for Brasilonema roberti-lamii (basionym: Tolypothrix roberti-lamii) from the French Antilles. Based on molecular data from DNA sequencing of the16S rRNA gene and the IGS of the cpcB-cpcA phycocyanin operon (cpcBA-IGS), we propose that the populations that we studied are closely related to those of other Brasilonema species, including B. octagenarum UFV-OR1, UFV-E1 and HA4187-MV1-p1F, Brasilonema sennae CENA 114, B. tolantongensis, B. terrestre CENA 116, B. angustatum HA4187-MV1-B2+p1F and HA4187-MV1-B2+p1H and B. bromeliae SPC951. Our findings support the transference of Tolypothrix roberti-lamii, which was made based exclusively on morphological criteria, to Brasilonema. The use of molecular analyses in addition to traditional morphological and ecological criteria, known as polyphasic approach, is a good alternative to describe taxa of cyanobacteria, mainly at the genus and species levels.

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