A new species of black fly (Diptera: Simuliidae) in the Simulium (Simulium) multistriatum species-group from Thailand

Zootaxa ◽  
2019 ◽  
Vol 4586 (3) ◽  
pp. 461 ◽  
Author(s):  
JIRAPORN THAIJARERN ◽  
KOMGRIT WONGPAKAM ◽  
ANONGRIT KANGRANG ◽  
PAIROT PRAMUAL
Keyword(s):  
New Species ◽  
Genetic Distance ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Pupal Stage ◽  
Species Group ◽  
Mountainous Area ◽  
Black Fly ◽  
Interspecific Genetic Distance

A new black fly species of the Simulium multistriatum species-group of the subgenus Simulium Latreille is described from the mountainous area in northeastern Thailand, based on morphology and mitochondrial DNA sequences. The new species is morphologically similar to S. laui Takaoka and Sofian-Azirun and S. lacduongense Takaoka and Ya’cob originally described from Vietnam, S. fenestratum Edwards originally described from Indonesia and S. chaliowae Takaoka and Boonkemtong originally described from Thailand, but can be distinguished in the adult stage by the number of upper eye facets and globular shape of the spermatheca and in the pupal stage by the cocoon and shape of thoracic tubercles. Genetic distance and phylogenetic analyses of mitochondrial cytochrome c oxidase I sequences differentiated the new species from other members of S. multistriatum species-group. All specimens of the new species formed a monophyletic clade with strong support in all phylogenetic analyses. The minimum interspecific genetic distance of 4.9% is considerably greater than the new species maximum intraspecific genetic distance (2.7%). 

scholarly journals Molecular and morphological evidence reveal a new genus and species in Auriculariales from tropical China

MycoKeys ◽  
2018 ◽  
Vol 35 ◽  
pp. 27-39 ◽  
Author(s):  
Hai-Sheng Yuan ◽  
Xu Lu ◽  
Cony Decock
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Type Species ◽  
New Genus ◽  
Phylogenetic Analyses ◽  
Strong Support ◽  
Morphological Characteristics ◽  
Morphological Evidence ◽  
New Genus And Species ◽  
Tropical China

Grammatuslabyrinthinusgen. et sp. nov. is proposed based on DNA sequences data and morphological characteristics. It is known so far from southern, tropical China. The new species is characterised by an annual, resupinate basidiocarp with a shallow, subporoid hymenophore, a hymenium restricted to the bottom of the tubes, a dimitic hyphal system, presence of encrusted skeletocystidia and dendrohyphidia, longitudinally septate basidia and smooth, oblong-ellipsoid to cylindrical, acyanophilous basidiospores. Phylogenetic analyses based on ITS + nLSU DNA sequences data indicate that G.labyrinthinus belongs to Auriculariaceae in which it has an isolated position. Phylogenetic inferences show G.labyrinthinus to be related to Heteroradulum. However, the ITS sequences similarity between G.labyrinthinus and H.kmetii, the type species of Heteroradulum, were 89.84% and support the establishment of the new genus. Inversely, Heteroradulumsemis clustered with G.labyrinthinus with strong support and it is transferred to Grammatus.

A new species of Brevipalpus (Acari: Tenuipalpidae) from the Azores Islands, with remarks on the B. cuneatus species group

2019 ◽  
Vol 24 (11) ◽  
pp. 2184-2208
Author(s):  
Jorge Laerson Dos Santos Alves ◽  
Francisco Ferragut ◽  
Renata Santos Mendonça ◽  
Aline Daniele Tassi ◽  
Denise Navia
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Nuclear Dna ◽  
Phylogenetic Analyses ◽  
Species Group ◽  
Wild Plants ◽  
Integrative Approach ◽  
Electron And Light Microscopy ◽  
Definition Of ◽  
A New Species

Brevipalpus contains about 290 species, some of which are considered of economic importance. In spite of their agricultural importance, the species diversity is scarcely known in several regions around the world, notably on wild plants. In this work a new species collected on an endemic ivy of the Azores Archipelago, Hedera azorica Carrière (Araliaceae), is described based on an integrative approach using morphological traits of the adults, obtained through electron and light microscopy, and molecular characters based on mitochondrial and nuclear DNA sequences. Phylogenetic analyses were conducted in order to produce reliable phylogenetic placement of the new species, which is tentatively classified in the B. portalis species group. Morphological similarities between the new species and B. cuneatus Canestrini and Fanzago point out to some inconsistencies in the current morphological classification of Brevipalpus species, especially in the definition of the B. cuneatus group, herein discussed.

A new black fly (Diptera: Simuliidae) species of the subgenus Asiosimulium Takaoka & Choochote from Thailand

Zootaxa ◽  
2018 ◽  
Vol 4388 (1) ◽  
pp. 111 ◽  
Author(s):  
UBON TANGKAWANIT ◽  
KOMGRIT WONGPAKAM ◽  
PAIROT PRAMUAL
Keyword(s):  
New Species ◽  
Genetic Distance ◽  
Sea Level ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
High Elevation ◽  
Geographic Region ◽  
Black Fly ◽  
Minimum Genetic Distance ◽  
Northeastern Thailand

A new black fly species of the subgenus Asiosimulium Takaoka & Choochote of the genus Simulium was recognized from northeastern Thailand based on morphology, mitochondrial DNA and ecology. This black fly species has similar morphological characteristics to Simulium oblongum Takaoka and Choochote that was also described from the same geographic region in all life stages. However, this new species could be distinguished at the adult stage by coloration of the maxillary palp and in the larval stage by the presence of a pigmented subesophageal ganglion that is lacking in S. oblongum. Genetic distance and phylogenetic analyses based on mitochondrial cytochrome c oxidase I sequences clearly differentiated the two species with minimum genetic distance of 3.51%. These species are also ecologically isolated as S. oblongum is found only at low elevation (<650 m above sea level) but the new species occurs only at high elevation (>1,100 m above sea level). 

Revision of Simulium rufibasis (Diptera: Simuliidae) in Japan and Korea: Chromosomes, DNA, and Morphology

2019 ◽  
Vol 57 (2) ◽  
pp. 388-403
Author(s):  
Peter H Adler ◽  
Masako Fukuda ◽  
Hiroyuki Takaoka ◽  
Will K Reeves ◽  
Sam-Kyu Kim ◽  
...  
Keyword(s):  
New Species ◽  
Genetic Distance ◽  
Sex Chromosomes ◽  
Mitochondrial Gene ◽  
Distinct Species ◽  
Species Group ◽  
The Status ◽  
Chromosomal Data ◽  
Interspecific Genetic Distance ◽  
A New Species

Abstract The widespread nominal black fly Simulium (Simulium) rufibasis Brunetti was reexamined morphologically, chromosomally, and molecularly to determine the status of populations in Japan and Korea with respect to S. rufibasis from the type locality in India and to all other known species in the S. (S.) tuberosum species-group. Morphological comparisons established that the species previously known as S. rufibasis in Japan and Korea is distinct from all other species. Consequently, it was described and illustrated as a new species, Simulium (S.) yamatoense. Simulium yokotense Shiraki, formerly a synonym of S. rufibasis, was morphologically reevaluated and considered a species unplaced to species-group in the subgenus Simulium. Chromosomal analyses of S. yamatoense sp. nov. demonstrated that it is unique among all cytologically known species of the S. tuberosum group and is the sister species of the Taiwanese species tentatively known as S. (S.) arisanum Shiraki. Populations of S. yamatoense sp. nov. included two cytoforms, based on the sex chromosomes. Cytoform A, including topotypical representatives, was found in Kyushu, Japan, whereas cytoform B was found in Korea and Honshu, Japan. Molecular analysis based on the COI mitochondrial gene generally corroborated morphological and chromosomal data that S. yamatoense sp. nov. is a distinct species and, like the chromosomal data, indicate that it is most closely related to S. arisanum, with interspecific genetic distance of 2.92–4.63%.

A new morphologically cryptic species of Phyllomedusa (Anura: Phyllomedusidae) from Amazonian forests of northern Peru revealed by DNA sequences

Zootaxa ◽  
2017 ◽  
Vol 4269 (2) ◽  
pp. 245 ◽  
Author(s):  
SANTIAGO CASTROVIEJO-FISHER ◽  
JÖRN KÖHLER ◽  
IGNACIO DE LA RIVA ◽  
JOSÉ M. PADIAL
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Species Group ◽  
Genetic Distances ◽  
Adult Males ◽  
16S Gene ◽  
Northern Peru ◽  
Amazonian Forests

We describe and name Phyllomedusa chaparroi sp. nov., a medium-sized species (snout–vent length in adult males 67.9–77.5 mm) of monkey frog from Amazonian rainforests of northern Peru. Although morphologically most similar to P. boliviana and P. camba (indistinguishable from the latter in external qualitative and quantitative traits), phylogenetic analysis of combined mitochondrial and nuclear markers place the new species sister to a clade containing P. neildi, P. tarsius, and P. trinitatis. Phyllomedusa chaparroi can be readily differentiated from these species by having a dark reddish-brown iris with indistinct tiny orange spots versus an orange iris with marked dark reticulation found in P. neildi, P. tarsius, and P. trinitatis. Furthermore, genetic distances for a 532 bp sequence of the 16S gene between the new species and its sister species are 2.8–4.1 %, whereas distances are 4.5–5.5 % to the morphologically cryptic P. camba. We briefly discuss the importance of DNA sequences in revealing morphologically cryptic species and modify the content of the P. tarsius species group based on phylogenetic analyses and observations on iris coloration.

A new species of Chlorociboria (Helotiales, Ascomycota) on herbaceous stems from China

Phytotaxa ◽  
2017 ◽  
Vol 312 (1) ◽  
pp. 111
Author(s):  
HUAN-DI ZHENG ◽  
WEN-YING ZHUANG
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Central China ◽  
Nuclear Ribosomal Dna ◽  
Light Blue ◽  
Ectal Excipulum ◽  
A New Species

A new species, namely Chlorociboria herbicola, is discovered on herbaceous stems in central China. Morphologically, the new fungus is distinctive by the combination of light blue-green apothecia, rectangular cells in ectal excipulum, and elongate-ellipsoidal ascospores with rounded ends. Phylogenetic analyses of the internal transcribed spacer and large subunit of nuclear ribosomal DNA sequences confirm its ascription in Chlorociboria and distinction from the known species of the genus.

scholarly journals Descriptions of four new dextral land snails of the genus Camaena (Gastropoda, Eupulmonata, Camaenidae) from south China

ZooKeys ◽  
2020 ◽  
Vol 996 ◽  
pp. 37-58
Author(s):  
Pei Wang ◽  
Mei-Ling Hu ◽  
Jun-Hong Lin ◽  
Hai-Fang Yang ◽  
Xiao-Jing Li ◽  
...  
Keyword(s):  
New Species ◽  
South China ◽  
Dna Sequences ◽  
Reproductive System ◽  
Phylogenetic Analyses ◽  
Morphological Characteristics ◽  
Land Snails ◽  
Shell Morphology ◽  
Molecular Phylogenetic ◽  
Living Environments

In this study, four new dextral camaenid from China are reported, based on shell morphology, reproductive system anatomy, and molecular phylogenetic analyses: Camaena funingensis Zhou, Wang &amp; Lin, sp. nov., Camaena gaolongensis Zhou, Wang &amp; Lin, sp. nov., Camaena maguanensis Zhou, Wang &amp; Hu, sp. nov., and Camaena yulinensis Zhou, Wang &amp; Hu, sp. nov. Detailed descriptions of the morphological characteristics including shells and genitalia, DNA sequences, and living environments of the four new species are provided, with further comparisons with congeners.

scholarly journals A new species of Xylophis Beddome, 1878 (Serpentes: Pareidae) from the southern Western Ghats of India

2021 ◽  
Vol 71 ◽  
pp. 219-230
Author(s):  
Surya Narayanan ◽  
Pratyush P. Mohapatra ◽  
Amirtha Balan ◽  
Sandeep Das ◽  
David J. Gower
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Genetic Distance ◽  
Dna Sequences ◽  
Western Ghats ◽  
White Collar ◽  
The Body ◽  
Western Ghats Of India ◽  
Southern Western Ghats ◽  
A New Species

We reassess the taxonomy of the Indian endemic snake Xylophis captaini and describe a new species of Xylophis based on a type series of three specimens from the southernmost part of mainland India. Xylophis deepakisp. nov. is most similar phenotypically to X. captaini, with which it was previously confused. The new species differs from X. captaini by having a broader, more regular and ventrally extensive off-white collar, more ventral scales (117–125 versus 102–113), and by lack of flounces on the body and proximal lobes of the hemipenis. Phylogenetic analysis of mitochondrial 16S DNA sequences strongly indicates that the new species is most closely related to X. captaini, differing from it by an uncorrected pairwise genetic distance of 4.2%. A revised key to the species of Xylophis is provided.

The Vietnamese population of Megophrys kuatunensis (Amphibia: Megophryidae) represents a new species of Asian horned frog from Vietnam and southern China

Zootaxa ◽  
2017 ◽  
Vol 4344 (3) ◽  
pp. 465 ◽  
Author(s):  
BENJAMIN TAPLEY ◽  
TIMOTHY CUTAJAR ◽  
STEPHEN MAHONY ◽  
CHUNG THANH NGUYEN ◽  
VINH QUANG DAU ◽  
...  
Keyword(s):  
New Species ◽  
Southern China ◽  
Phylogenetic Analyses ◽  
Eastern China ◽  
Taxonomic Status ◽  
Species Group ◽  
Type Specimens ◽  
Vietnamese Population ◽  
Group A ◽  
A New Species

The Asian frog genus Megophrys is a diverse group of morphologically conserved, forest-dwelling frogs. The genus harbours highly localised species diversification and new species continue to be described on a regular basis. We examined the taxonomic status of a population of Megophrys frogs from the Hoang Lien Range in northern Vietnam and southern China previously identified as M. kuatunensis (subgenus Panophrys). Preliminary phylogenetic analyses using a fragment of 16S rDNA places the species in question within the Megophrys (subgenus Panophrys) species group, a primarily Chinese radiation within the genus. On the basis of morphological, molecular and bioacoustic data, we conclude that this population does not represent M. kuatunensis, or any known species in the genus. We herein describe this species of Megophrys as new. Known only from Sa Pa District, Lao Cai Province in Vietnam and Jinping County, Yunnan Province in China, the new species is likely to be threatened by ongoing deforestation in the region. We provide an updated species description of M. kuatunensis based on type specimens, and suggest that M. kuatunensis is likely to be restricted to eastern China.  

scholarly journals Morphological and molecular identification of a new species of Atraporiella (Polyporales, Basidiomycota) in China

Phytotaxa ◽  
2017 ◽  
Vol 332 (1) ◽  
pp. 31 ◽  
Author(s):  
ZI-QIANG WU ◽  
SHAN SHEN ◽  
KAI-YUE LUO ◽  
ZHENG-HUI WANG ◽  
CHANG-LIN ZHAO
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Strong Support ◽  
Molecular Data ◽  
Fungal Species ◽  
Monophyletic Lineage ◽  
Inference Methods ◽  
A New Species ◽  
Molecular Characters

A new poroid wood-inhabiting fungal species, Atraporiella yunnanensis sp. nov., is proposed based on morphological and molecular characters. The species is characterized by cream pore surface when dry, which is easy to separate from substrate and very rapidly stained dark brown to black when bruised; hyphal system monomitic with generative hyphae hyaline to pale brown, thin-walled, unbranched, interwoven; slightly allantoid basidiospores, 2.2–3 × 0.8–1.5 µm. The internal transcribed spacer (ITS) and the large subunit (LSU) regions of nuclear ribosomal RNA gene sequences of the studied samples were generated, and phylogenetic analyses were performed with maximum likelihood, maximum parsimony and bayesian inference methods. The phylogenetic analysis based on molecular data of ITS+nLSU sequences showed that Atraporiella yunnanensis belonged to the residual polyporoid clade, formed a monophyletic lineage with a strong support (100% BS, 100% BP, 1.00 BPP) and was closely related to A. neotropica, and then grouped with other related genera: Antrodiella, Pouzaroporia, Steccherinum and Xanthoporus. Both morphological and molecular characters confirmed the placement of the new species in Atraporiella.

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