scholarly journals First record of Bursaphelenchus hildegardae Braasch et al., 2006 (Nematoda) in New Zealand with updated information on morphology, sequencing and a key to species of the eggersi-group

Zootaxa ◽  
2021 ◽  
Vol 5071 (1) ◽  
pp. 151-165
Author(s):  
ZENG QI ZHAO ◽  
MICHAEL SURREY ◽  
WELLCOME HO ◽  
MILEN MARINOV ◽  
CAROLYN BLEACH ◽  
...  
Keyword(s):  
New Zealand ◽  
Internal Transcribed Spacer ◽  
New Record ◽  
Large Subunit ◽  
First Record ◽  
Small Subunit ◽  
Key To Species ◽  
Internal Transcribed Spacer Region ◽  
Adult Body ◽  
Hylastes Ater

Bursaphelenchus hildegardae Braasch et al., 2006 was collected from pine wood (Pinus radiata) growing in Kaingaroa Timberlands, and a bark beetle, Hylastes ater Paykull, 1800 in New Zealand. This is a new record for B. hildegardae, occuring in New Zealand, and the second report from the southern hemisphere in addition to Australia. In general, the New Zealand isolate of B. hildegardae corresponds well with the description of B. hildegardae given by Braasch et al. (2006) from Germany. The New Zealand isolate is characterized by having an adult body length of 807–1190 μm, medium a ratios (47.5–58.5 for female and 44.6–60.1 for male), b ratios of 9.8–14.5 (female) and 10.2–12.7 (male), c ratios of 18.8–25.2 (female) and 21.6–32.4 (male), c’ ratios of 4.0–4.4 (female) and 2.1–2.7 (male), and is characterised by having three incisures in the lateral fields, thorn-shaped spicules with a distinctly dorsally-bent thin hook-like condylus, and a dorso-ventally visible terminal bursa. In addition, molecular phylogeny using near full length small subunit (SSU), D2/D3 expansion segments of the large subunit (LSU) and the internal transcribed spacer region (ITS1 and 2) of the ribosomal rDNA supports the identification. A key to Bursaphelenchus species in the eggersi-group is given.  

First record of the root knot nematode, Meloidogyne minor in New Zealand with description, sequencing information and key to known species of Meloidogyne in New Zealand 

Zootaxa ◽  
2017 ◽  
Vol 4231 (2) ◽  
pp. 203 ◽  
Author(s):  
ZENG QI ZHAO ◽  
WELLCOME HO ◽  
RUTH GRIFFIN ◽  
MICHAEL SURREY ◽  
ROBERT TAYLOR ◽  
...  
Keyword(s):  
New Zealand ◽  
Large Subunit ◽  
Intergenic Spacer ◽  
First Record ◽  
Small Subunit ◽  
Root Knot Nematode ◽  
Head Region ◽  
Internal Transcribed Spacer Region ◽  
Stage Juvenile ◽  
Labial Disc

Meloidogyne minor Karssen et al. 2004 was collected from perennial ryegrass (Lolium perenne L.) growing in a sports ground in Christchurch, New Zealand. This is a new record for M. minor, the first report of this nematode occurring in New Zealand, and the second report from the southern hemisphere (after Chile). In general, the New Zealand isolate of M. minor corresponds well to the descriptions of M. minor given by Karssen et al. (2004). The New Zealand isolate is characterized by having a female with dorsally curved stylet, 13–14 μm long, with transversely ovoid knobs slightly sloping backwards from shaft; rounded perineal pattern; and male with stylet 16–19 μm long, large transversely ovoid knobs sloping slightly backwards from shaft; head region not set off, labial disc elevated, lateral lips prominent; and second stage juvenile 370–390 μm long, with hemizonid posterior but adjacent to excretory pore; tail 53–63 μm long; and a distinct hyaline tail terminus 14–18 μm long. In addition, molecular phylogeny using near full length small subunit (SSU), D2/D3 expansion segments of the large subunit (LSU), the internal transcribed spacer region (ITS1 and 2), and the intergenic spacer (IGS2) of the ribosomal rDNA supports the identification. 

scholarly journals Phylogenetic Trees of Aecial-Stage Rust Fungus, Puccinia paederiae (Dietel) Gorlenko Causing Gall on Paederia linearis Hook f.

2017 ◽  
Vol 15 (10) ◽  
pp. 739-752
Author(s):  
Wuttiwat JITJAK ◽  
Niwat SANOAMUANG
Keyword(s):  
Bayesian Methods ◽  
Dna Sequences ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Trees ◽  
Rust Fungus ◽  
Large Subunit ◽  
Molecular Data ◽  
Small Subunit ◽  
Phylogenetic Study ◽  
Internal Transcribed Spacer Region

A rust fungus, Puccinia paederiae (Dietel) Gorlenko causing galls on the stem of the skunk vine (Paederia linearis Hook. f. var. linealis and P. linealis var. palida (Craib) Puff) was collected for phylogenetic study as no molecular data was exclusively available for this fungus. Three regions of ribosomal DNA sequences, small subunit (SSU), large subunit (LSU) and internal transcribed spacer region 1 (ITS1) were employed. The results of maximum parsimony and Bayesian methods suggested that among the trees with these sequences, this fungus was nested in Pucciniaceae clades and Puccinia species with supportive statistical values. This is the first report on the phylogenetic analysis using multiple genes of the rust, P. paederiae.

Molecular phylogeny of two coelomycetous fungal genera with stellate conidia,ProsthemiumandAsterosporium, on Fagales trees

Botany ◽  
2010 ◽  
Vol 88 (12) ◽  
pp. 1057-1071 ◽  
Author(s):  
Kazuaki Tanaka ◽  
Vadim A. Mel’nik ◽  
Maasa Kamiyama ◽  
Kazuyuki Hirayama ◽  
Takashi Shirouzu
Keyword(s):  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Diagnostic Value ◽  
Small Subunit ◽  
Nuclear Ribosomal Dna ◽  
Internal Transcribed Spacer Region ◽  
Phylogenetic Relatedness ◽  
Close Relationship ◽  
Phylogenetic Affinities ◽  
A New Species

Prosthemium (teleomorph Pleomassaria ) and Asterosporium (teleomorph unknown) are coelomycetous genera with stellate conidia on Fagales trees. Their morphological resemblance suggests their close relationship, but phylogenetic relatedness remains unknown. They have been distinguished on the basis of either conidiomatal morphology (pycnidia in Prosthemium and acervuli in Asterosporium) or their differing conidial septation (euseptate in Prosthemium and distoseptate in Asterosporium). To reveal their phylogenetic affinities and clarify reliable distinguishing phenotypical characters, five species of Prosthemium and two species of Asterosporium were investigated using sequences of the small subunit, large subunit, and internal transcribed spacer region of nuclear ribosomal DNA and β-tubulin gene from 43 isolates of these species. The analyses revealed the following: (i) Asterosporium typified by Asterosporium asterospermum on Fagus is a member of the Sordariomycetes and is distinct from Prosthemium belonging to the Dothideomycetes; (ii) Asterosporium betulinum on Betula species should be excluded from Asterosporium and transferred to Prosthemium as Prosthemium neobetulinum nom. nov.; (iii) conidial septation does not seem to have a diagnostic value, whereas conidiomatal morphology is useful in distinguishing both genera; (iv) the number and length of conidial arms are useful criteria in distinguishing Prosthemium species. A new species, Prosthemium intermedium sp. nov., found on both Betula ermanii and Betula maximowicziana , is described and illustrated.

A new species and a new record of Parasola from Kerala State, India

Phytotaxa ◽  
2018 ◽  
Vol 369 (4) ◽  
pp. 260 ◽  
Author(s):  
K. G. GREESHMA GANGA ◽  
PATINJAREVEETTIL MANIMOHAN
Keyword(s):  
New Species ◽  
Maximum Likelihood ◽  
Internal Transcribed Spacer ◽  
New Record ◽  
Large Subunit ◽  
Phylogenetic Study ◽  
Internal Transcribed Spacer Region ◽  
Kerala State ◽  
Micro Structures ◽  
A New Species

Two species of Parasola, P. parvula sp. nov. and P. lilatincta are described from Kerala State, India, based on both morphology and molecular phylogeny. Descriptions with photographs of both the basidiocarps and the micro-structures and comparison with morphologically similar and phylogenetically related species are provided. Sequences of both the nuclear ribosomal internal transcribed spacer region (ITS) and the nuclear ribosomal large subunit (nLSU) region of the two species were obtained and used in BLASTn searches. Phylogenetic study was based on Maximum likelihood (ML) analysis of the ITS sequences.

Amanita mansehraensis, a new species in section Vaginatae from Pakistan

Phytotaxa ◽  
2019 ◽  
Vol 409 (4) ◽  
pp. 189-201 ◽  
Author(s):  
MALKA SABA ◽  
DANNY HAELEWATERS ◽  
MUHAMMAD FIAZ ◽  
ABDUL NASIR KHALID ◽  
DONALD H. PFISTER
Keyword(s):  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Large Subunit ◽  
Pine Forests ◽  
Evolutionary Relationships ◽  
Internal Transcribed Spacer Region ◽  
Bayesian Inferences ◽  
A New Species

A new species of Amanita subgenus Amanita sect. Vaginatae is described and illustrated based on material collected in pine forests in district Mansehra, Khyber Pakhtoonkhaw, Pakistan. Amanita mansehraensis is recognized by the presence of a light brown or light greyish olive pileus with strong brown or deep brown pileus center; non-appendiculate, rimose, sulcate or plicate striate pileus margin; subglobose to ellipsoid basidiospores; and a saccate volva. The internal transcribed spacer region (ITS) and large subunit of the nuclear ribosomal RNA gene (nrLSU) were used for the delimitation of this species based on sequence data. The evolutionary relationships of A. mansehraensis with other species of Amanita were inferred by means of Maximum Likelihood and Bayesian inferences of the nrLSU dataset and concatenated ITS+nrLSU dataset. Amanita mansehraensis is most closely related to A. brunneofuliginea, A. pseudovaginata, and the recently described A. glarea.

Litylenchus coprosma gen. n., sp. n. (Tylenchida: Anguinata), from leaves of Coprosma repens (Rubiaceae) in New Zealand

Nematology ◽  
2011 ◽  
Vol 13 (1) ◽  
pp. 29-44 ◽  
Author(s):  
Kerrie Davies ◽  
Zeng Qi Zhao ◽  
Brett Alexander ◽  
Ian Riley
Keyword(s):  
New Zealand ◽  
New Genus ◽  
Large Subunit ◽  
Small Subunit ◽  
Rrna Genes ◽  
New Genus And Species ◽  
Pore Opening ◽  
Terminal Bulb ◽  
Stylet Length ◽  
Tail Tip

AbstractA new genus and species of anguinid nematode, Litylenchus coprosma gen. n., sp. n., was recovered from leaves of Coprosma repens A. Rich. from an amenity planting in Wellington, New Zealand. The genus is characterised by having slender males and slender or semi-obese females, pharynx with a weak non-muscular median bulb, a terminal bulb containing the pharyngeal glands, female with a single gonad having a quadricolumella and post-uterine sac; male with arcuate spicules and the bursa arising 1-2 anal body diam. anterior to the cloacal aperture and extending nearly to the tail tip, and does not induce galls, only foliar chlorosis. The species is characterised by having a short, robust stylet with conus forming ca 40% of stylet length and three well developed rounded knobs, secretory/excretory pore opening posterior to the nerve ring, terminal bulb abutting the intestine, and tail tip of variable form. Molecular phylogeny of near full length small subunit, D2/D3 expansion segments of the large subunit and internal transcribed spacer rRNA genes support the description of L. coprosma gen. n., sp. n. as a new genus and species.

First report of a gryporhynchid tapeworm (Cestoda: Cyclophyllidea) from New Zealand and from an eleotrid fish, described from metacestodes and in vitro-grown worms

2011 ◽  
Vol 86 (4) ◽  
pp. 453-464 ◽  
Author(s):  
B. Presswell ◽  
R. Poulin ◽  
H.S. Randhawa
Keyword(s):  
New Zealand ◽  
First Record ◽  
Small Subunit ◽  
Body Cavity ◽  
The Body ◽  
Species Determination ◽  
Fish Family ◽  
Rdna Sequences ◽  
Stage Of Development

AbstractMetacestodes are often found in the body cavity of the common bully (Gobiomorphus cotidianus McDowall), from freshwater habitats in Otago, New Zealand. Identification of metacestodes relies only on the number, size and shape of the rostellar hooks. To attempt species determination, we cultivated metacestodes in vitro for up to 23 days, during which they matured to at least the male stage of development, although female organs were not discernable. Identified as members of the genus Paradilepis Hsü, 1935 (family Gryporhynchidae), these specimens are compared to previously described species, in particular P. minima (Goss, 1940), from Australia, the closest species, both geographically and morphologically. Although the size of scolex, suckers and proglottids differ significantly from those of P. minima, we are cautious about interpreting ‘adults’ grown in vitro, because we are unsure whether the artificial conditions alter development. For this reason, and because of the lack of female organs, we refrain from erecting a new species, and refer to the specimens as Paradilepis cf. minima until such time as the adults are found in the definitive host. With this proviso we present here a description of the in vitro-grown worms and the metacestodes as a preliminary study of this cestode. A molecular analysis of small subunit (SSU) rDNA sequences, shows the position of P. cf. minima and another gryporhynchid, Neogryporhynchus cheilancristrotus (Wedl, 1855), to be equivocal, but confirms their exclusion from the Dilepididae and Hymenolepididae. This is the first record of a gryporhynchid from New Zealand, and the first from the fish family Eleotridae.

Deladenus nitobei n. sp. (Tylenchomorpha: Allantonematidae) isolated from Sirex nitobei (Hymenoptera: Siricidae) from Aomori, Japan, a new member of the siricidicola superspecies

Nematology ◽  
2016 ◽  
Vol 18 (10) ◽  
pp. 1199-1217 ◽  
Author(s):  
Natsumi Kanzaki ◽  
Suguru E. Tanaka ◽  
Katrin Fitza ◽  
Hajime Kosaka ◽  
Bernard Slippers ◽  
...  
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Pinus Densiflora ◽  
Large Subunit ◽  
Small Subunit ◽  
Body Cavity ◽  
The Body ◽  
Malt Extract ◽  
Internal Transcribed Spacer Region ◽  
Mtcoi Gene

Deladenus nitobein. sp., a parasite of a woodwasp species,Sirex nitobei, is described based on its typological characters and molecular profiles of part of the small subunit D2-D3 expansion segments of the large subunit and internal transcribed spacer region of the ribosomal RNA gene, as well as part of the mitochondrial cytochrome oxidase subunit I (mtCOI) gene. Adult host woodwasps emerging from dead Japanese red pine logs,Pinus densiflora, collected at Aomori, Japan, were dissected and examined for nematode association. The new species was isolated from the body cavity and reproductive system ofS. nitobeias large parasitic females and small parasitic juveniles. The nematodes were cultured successfully on 1.0% malt extract agar medium, inoculated withSirex-associated fungus,Amylostereumareolatum. The mycophagous adult nematodes were characterised by the relative position of the excretory pore, located at 25 (19-28) and 25 (18-30)μm anterior to the hemizonid in the male and female, respectively, and a broad female tail with a rounded distal end. Typologically, the new species forms a cryptic species complex withD. siricidicolaandD. canii. In addition, the new species andD. siricidicolashare the same host wasp, tree and fungal species in Japan. However, the cryptic species can be separated from each other based on the described morphological and molecular sequence differences in the mtCOI gene.

Cortinarius rapaceoides, a new record for Turkey

Mycotaxon ◽  
2020 ◽  
Vol 135 (3) ◽  
pp. 559-568
Author(s):  
Meryem Senay Şengul Demirak ◽  
Hakan Işik
Keyword(s):  
Phylogenetic Relationships ◽  
Internal Transcribed Spacer ◽  
New Record ◽  
Sequence Data ◽  
Large Subunit ◽  
Morphological Description ◽  
Molecular Evidence

A macrofungus collected from Akbelen village in Tokat has been identified based on molecular evidence and morphology as Cortinarius rapaceoides, a new record from Turkey. Sequence data generated from the nuclear ribosomal internal transcribed spacer and ribosomal large subunit regions were deposited in GenBank and used to determine phylogenetic relationships within Cortinarius subg. Phlegmacium. A morphological description, illustrations, and phylogeny of the Turkish C. rapaceoides are provided.

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