scholarly journals Effect of Aerated and Non-aerated Compost Steepages on the Severity and Incidence of Major Fungal Diseases of Faba Bean; <i>Botrytis fabae</i>, <i>Uromyces vicia fabae</i> and <i>Ascochyta fabae</i>

Plant ◽  
2017 ◽  
Vol 5 (6) ◽  
pp. 85 ◽  
Author(s):  
Addisu Tegegn
Keyword(s):  
Faba Bean ◽  
Fungal Diseases ◽  
Botrytis Fabae ◽  
Ascochyta Fabae

scholarly journals Photosystem II Repair Cycle in Faba Bean May Play a Role in Its Resistance to Botrytis fabae Infection

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2247
Author(s):  
María Ángeles Castillejo ◽  
Ángel M. Villegas-Fernández ◽  
Tamara Hernández-Lao ◽  
Diego Rubiales
Keyword(s):  
Faba Bean ◽  
Molecular Mechanisms ◽  
Crop Yields ◽  
Primary Metabolism ◽  
Protein Repair ◽  
Two Dimensional Gel Electrophoresis ◽  
Botrytis Fabae ◽  
Metabolic Imbalance ◽  
Response To Stress ◽  
Repair Cycle

Chocolate spot, which is caused by the necrotrophic fungus Botrytis fabae, is a major foliar disease occurring worldwide and dramatically reducing crop yields in faba bean (Vicia faba). Although chemical control of this disease is an option, it has serious economic and environmental drawbacks that make resistant cultivars a more sensible choice. The molecular mechanisms behind the defense against B. fabae are poorly understood. In this work, we studied the leave proteome in two faba bean genotypes that respond differently to B. fabae in order to expand the available knowledge on such mechanisms. For this purpose, we used two-dimensional gel electrophoresis (2DE) in combination with Matrix-Assisted Laser Desorption/Ionization (MALDI-TOF/TOF). Univariate statistical analysis of the gels revealed 194 differential protein spots, 102 of which were identified by mass spectrometry. Most of the spots belonged to proteins in the energy and primary metabolism, degradation, redox or response to stress functional groups. The MS results were validated with assays of protease activity in gels. Overall, they suggest that the two genotypes may respond to B. fabae with a different PSII protein repair cycle mechanism in the chloroplast. The differences in resistance to B. fabae may be the result of a metabolic imbalance in the susceptible genotype and of a more efficient chloroplast detoxification system in the resistant genotype at the early stages of infection.

scholarly journals Application of molecular methods for characterization of Botrytis fabae and Botrytis cinerea of faba bean

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 314-318
Author(s):  
N.M. Abou-Zeid ◽  
I.H. Dorriah ◽  
A.A. Marwa
Keyword(s):  
Botrytis Cinerea ◽  
Faba Bean ◽  
Morphological Characters ◽  
Molecular Methods ◽  
Pcr Analysis ◽  
Conidial Morphology ◽  
Rapd Pcr ◽  
Botrytis Fabae

Application of the RAPD methods allowed to clearly characterization of isolates of Botrytis fabae and isolates of B. cinerea. Results from RAPD-PCR analysis indicate different groups. Clusters were related with groups based on conidial morphology, morphological characters of the isolates of Botrytis spp.

Screening of Faba Bean (Vicia faba L.) Germplasm Accessions against Grey Mould Disease Caused by Botrytis fabae Sard

2020 ◽  
Author(s):  
Ibrahim Elkhalil Benzohra ◽  
Hakima Belaidi
Keyword(s):  
Vicia Faba ◽  
Faba Bean ◽  
Grey Mould ◽  
Grain Legume ◽  
Sources Of Resistance ◽  
Significant Difference ◽  
Botrytis Fabae ◽  
Vicia Faba L ◽  
Faba Beans ◽  
Chocolate Spot Disease

Background: Faba bean (Vicia faba L.) is thirst most important grain legume in the world and the first one in Algeria. The chocolate spot disease caused by Botrytis fabae Sard (BF), is the major constraint of this culture in Algeria when caused a destructive damages.Methods: The aim of this study is to find the sources of resistance for Seven varieties of faba beans (Giza 02, Giza 40, Giza 461, Sakha 02, Sakha 03, Precoce de Seville), by using the detached leaflet inoculation test for resistance to the two isolates from BF represent two different regions of northwest Algeria (Mascara and Relizane). Result: A significant difference (P less than 0.05) was observed in the reaction of the varieties which manifest themselves by a different reaction vis-à-vis the isolates of BF. Total sensitivity was observed in the four (4) varieties Giza02, Sakha01, Sakha02 and Sakha03, two (2) varieties (Giza40 and Ziban), are tolerant, while the variety named ‘Precoce de Seville’ showed significant resistance to both isolates from BF. These results showed a similar pathogenic behavior of two isolates of BF and variability in the level of reactions of the varieties of beans. The variety ‘Precoce de Seville’ showed promising results to be valued and cultivated in order to reduce the damage caused by this disease and reduce the use of chemicals.

scholarly journals Ascochyta fabae and A. lentis: Host Specificity, Teleomorphs (Didymella), Hybrid Analysis, and Taxonomic Status

Plant Disease ◽  
1997 ◽  
Vol 81 (7) ◽  
pp. 809-816 ◽  
Author(s):  
W. J. Kaiser ◽  
B.-C. Wang ◽  
J. D. Rogers
Keyword(s):  
Faba Bean ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Morphological Characteristics ◽  
Taxonomic Status ◽  
Fungal Species ◽  
Hybrid Progeny ◽  
Banding Patterns ◽  
Pathogenicity Tests ◽  
Ascochyta Fabae

Isolates of Ascochyta fabae from faba bean (Vicia faba) and A. lentis from lentil (Lens culinaris) collected from different countries were used in this study. The Didymella teleomorph (sexual state) of each fungus was induced to develop and mature on inoculated sterile lentil stems. Both fungi were heterothallic, with two mating types, designated MAT1-1 and MAT1-2. When certain isolates of A. fabae and A. lentis were crossed, hybrid pseudothecia developed. Growth, sporulation, colony appearance, morphology, and pathogenicity of the hybrid progeny frequently differed greatly from the parent isolates. Inoculations with single-ascospore progeny from matings among compatible isolates of A. fabae caused disease in faba bean but not in lentil; inoculations with single-ascospore progeny from matings among compatible isolates of A. lentis incited disease in lentil but not in faba bean. Inoculations with single-ascospore progeny from crosses between faba bean and lentil isolates did not induce disease in either host. Asci from crosses between A. fabae and A. lentis mostly contained fewer than eight ascospores that were, on average, larger than those from eight-spored asci. Matings among certain isolates of A. fabae resulted in production of pseudothecia with ascospores considerably larger than is typical for D. fabae. Random amplified polymorphic DNA (RAPD) banding patterns of Ascochyta isolates from faba bean and lentil are clearly different, and banding patterns from hybrid progeny from crosses between A. fabae and A. lentis confirmed hybridity. RAPD markers proved useful in supporting identifications of ascospore isolates from faba bean to known Ascochyta species. Dendrogram analysis indicated similarity between the two fungal species was low. The pathogenicity tests, morphological characteristics, and RAPD markers indicate that A. fabae and A. lentis represent distinct taxa. D. lentis, with its anamorph, A. lentis, is proposed as a new species that is distinct from D. fabae, with its anamorph, A. fabae.

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