Comparison of MicroRNA Expression in Aqueous Humor of Normal and Primary Open-Angle Glaucoma Patients Using PCR Arrays: A Pilot Study

Abstract Purpose: This study was aimed to analyze the profile of vascular and inflammatory cytokines in aqueous humor of primary open angle glaucoma (POAG) patients. Methods: Aqueous humor samples were acquired from 6 POAG patients and 7 age-related cataract patients that constituted the control group. A human cytokine antibody array was used to detect 37 proteins related to inflammation and vascular regulation. Characteristics including age, gender, and intraocular pressure (IOP) of POAG and age-related cataract patients were compared. Correlation analyses between aqueous humor cytokines and characteristics were performed. Results: The cytokine antibody array results showed that the signal intensities of soluble vascular endothelial growth factor receptor 1 (sVEGFR-1), von Willebrand factor (vWF), tumor necrosis factor (TNF)-α, and angiopoietin-2 in POAG patients were significantly higher in aqueous samples compared to controls (p < 0.05, Student’s test or Mann-Whitney U-test). Among cytokines of all aqueous samples, preoperative IOP was positively associated with expression quantity of sVEGFR-1 (r = 0.647, p = 0.02) and VEGF-A (r = 0.602, p = 0.04) and negatively associated with plasminogen activator inhibitor 1 expression (r = -0.593, p = 0.04). Values for sVEGFR-1 were positively correlated with vWF (r = 0.646, p = 0.02) and TNF-α (r = 0.824, p < 0.001) with statistical significance. Conclusion: Pathogenesis of primary open angle glaucoma may be associated with both vascular dysfunction and inflammatory responses. The vascular factors may include endothelial dysfunction and damaged vascular permeability as indicated by abnormal expression of vWF, sVEGFR-1 and angiopoietin-2. Keywords: POAG, aqueous humor, cytokines, sVEGFR-1, vWF, angiopoietin-2

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Influence of Selective Laser Trabeculoplasty on 24-Hour Diurnal Intraocular Pressure Fluctuation in Primary Open-Angle Glaucoma: A Pilot Study

Ophthalmic Surgery Lasers and Imaging ◽

10.3928/15428877-20100430-08 ◽

2010 ◽

Vol 41 (3) ◽

pp. 342-347 ◽

Cited By ~ 19

Author(s):

Péter Kóthy ◽

Márta Tóth ◽

Gábor Holló

Keyword(s):

Intraocular Pressure ◽

Pilot Study ◽

Pressure Fluctuation ◽

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Open Angle ◽

Selective Laser Trabeculoplasty ◽

Laser Trabeculoplasty ◽

Intraocular Pressure Fluctuation

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Comparison of cytokine/chemokine levels in aqueous humor of primary open-angle glaucoma patients with positive or negative outcome following trabeculectomy

Bioscience Reports ◽

10.1042/bsr20181894 ◽

2019 ◽

Vol 39 (5) ◽

Cited By ~ 4

Author(s):

Beata Gajda-Deryło ◽

Thomas Stahnke ◽

Stephan Struckmann ◽

Gregor Warsow ◽

Kerstin Birke ◽

...

Keyword(s):

Growth Factor ◽

Aqueous Humor ◽

Transforming Growth Factor ◽

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Transforming Growth Factor Β ◽

Positive Outcome ◽

Open Angle ◽

Antifibrotic Therapy ◽

Cytokines And Chemokines

Abstract We aimed to identify differences in cytokine/chemokine levels in the aqueous humor (AH) of primary open-angle glaucoma (POAG) patients who suffered from scarring, compared with POAG patients with no scarring after trabeculectomy surgery. Identification of differently expressed cytokines and chemokines may help to understand scarring and fibrotic processes following trabeculectomy, and to make predictions for the outcome of fistulating surgery in the future. Furthermore, the identification of cell signaling pathways involved in fibrosis offers the opportunity for a more specific antifibrotic therapy with reduced side effects, and an improvement in long-term surgical outcome. Eight samples of AH were collected during trabeculectomy surgery and commercially available cytokine/chemokine arrays were used. Specific, differently expressed proteins (cytokines/chemokines) in AH samples from patients with positive and negative surgery outcomes were detected. These proteins were classified based on their known profibrotic, inflammatory, adhesive, and apoptotic properties. Transforming growth factor β (TGF-β) and vascular endothelial growth factor (VEGF) were among the most important profibrotic cytokines that we detected. Differences in the fold change of protein expression were highly significant between patients after successful and failed trabeculectomy surgery, and these were processed and visualized using ExprEssence software. This pilot study revealed differences in concentrations of cytokines/chemokines in AH between the two examined groups of patients. Our findings suggest that a positive outcome from trabeculectomy is strongly related to an inhibition of the fibrosis process.

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Aqueous humor metabolite profile of pseudoexfoliation glaucoma is distinctive

Molecular Omics ◽

10.1039/c9mo00192a ◽

2020 ◽

Vol 16 (5) ◽

pp. 425-435 ◽

Cited By ~ 1

Author(s):

Ciara Myer ◽

Leila Abdelrahman ◽

Santanu Banerjee ◽

Ram B. Khattri ◽

Matthew E. Merritt ◽

...

Keyword(s):

Machine Learning ◽

Aqueous Humor ◽

Primary Open Angle Glaucoma ◽

Open Angle Glaucoma ◽

Metabolite Profile ◽

Open Angle ◽

Pseudoexfoliation Glaucoma

We identified 298 metabolites in pseudoexfoliation (PEX) glaucoma, primary open angle glaucoma (POAG) and non-glaucomatous controls. Machine learning can classify aqueous humor into the three distinct categories and presents the opportunity for future predictions.

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