Induction of Endolymphatic Hydrops by Directly Infused Monoclonal Antibody against Type Ii Collagen CB11 Peptide

2002 ◽  
Vol 111 (7) ◽  
pp. 587-592 ◽  
Author(s):  
Hiroshi Matsuoka ◽  
Soon Seog Kwon ◽  
Marco Barbieri ◽  
Yoshiro Yazawa ◽  
T. J. Yoo
Keyword(s):  
Monoclonal Antibodies ◽  
Guinea Pigs ◽  
Auditory Evoked Potentials ◽  
Endolymphatic Hydrops ◽  
Hearing Threshold ◽  
Type Ii Collagen ◽  
Mouse Serum ◽  
Control Group ◽  
Type Ii ◽  
Scala Tympani

Autoimmune ear disease induced by type II collagen has been investigated by Yoo et al. In the present study, we investigated the effects of direct chronic infusion of type II collagen-specific monoclonal antibodies into guinea pig cochlea. Type II collagen fragment CB11 peptide-specific monoclonal antibodies (anti-CB11 Mabs) were infused directly into the scala tympani of guinea pigs with an Alzet mini-osmotic pump (anti-CB11 Mab group). As a control, normal mouse serum was infused by the same method (control group). To evaluate the auditory function, we recorded brain stem auditory-evoked potentials (BAEPs). In the anti-CB11 Mab group, 80% of the animals showed an increased hearing threshold of more than 25 dB at 7 days after infusion. The hearing threshold shift observed in the guinea pigs of the control group was minimal (15 dB or less). To detect the structural changes, we performed histopathologic studies using hematoxylin and eosin staining. Inflammatory cell migration was detected mainly in the scala tympani of the guinea pigs of both groups. In the anti-CB11 Mab group, endolymphatic hydrops was also observed. The results of this experiment suggest that type II collagen autoimmunity is responsible for the production of hearing loss and endolymphatic hydrops.

Electrocochleographic Changes in Endolymphatic Hydrops Induced by Type II Collagen Immunization through the Stylomastoid Foramen

1989 ◽  
Vol 98 (7) ◽  
pp. 556-562 ◽  
Author(s):  
Toru Ohashi ◽  
Koichi Tomoda ◽  
Nobuo Yoshie
Keyword(s):  
Ganglion Cell ◽  
Guinea Pigs ◽  
Endolymphatic Hydrops ◽  
Spiral Ganglion ◽  
Type Ii Collagen ◽  
Ménière’S Disease ◽  
Type Ii ◽  
Cell Degeneration ◽  
Stylomastoid Foramen ◽  
Spiral Ganglion Cell

Changes in action potential (AP) and summating potential (SP) were investigated in guinea pigs immunized with type II collagen through the stylomastoid foramen. Endolymphatic hydrops could be induced in four of 11 guinea pigs. The striking feature of the electrocochleographic waveform in guinea pigs with endolymphatic hydrops was the negative SP recording in response to high frequency tone bursts. Furthermore, abnormal changes in AP were observed in three of four hydropic guinea pigs. Morphologic study of the cochleas in these three guinea pigs with light microscopy revealed spiral ganglion cell degeneration in addition to endolymphatic hydrops and almost normal sensory hair cells. These results suggest that guinea pigs with hydrops as produced by our procedure can serve as a useful model of Meniere's disease, that autoimmune response may play an important role in the etiopathogenesis of Meniere's disease, and that spiral ganglion cell degeneration together with endolymphatic hydrops seems to contribute to abnormal changes in AP.

scholarly journals Demonstration of immunoreactive sites on cartilage after in vivo administration of biotinylated anti-type II collagen antibodies.

1989 ◽  
Vol 37 (2) ◽  
pp. 265-268 ◽  
Author(s):  
R Jonsson ◽  
A L Karlsson ◽  
R Holmdahl
Keyword(s):  
Monoclonal Antibodies ◽  
Simple Technique ◽  
Specific Binding ◽  
Type Ii Collagen ◽  
Type Ii ◽  
Cytochemical Staining ◽  
Collagen Antibodies ◽  
Detection And Localization ◽  
In Vivo Administration

Administration of biotinylated monoclonal antibodies provides the basis of a simple technique for identifying immunoreactive sites in vivo. Biotinylated anti-type II collagen antibodies were injected intraperitoneally into normal DBA/1 mice. The mice were sacrificed after 96 hr and the front paws removed and decalcified to allow tissue sectioning before snap-freezing. Binding of antibodies in vivo was visualized with affinity cytochemical staining using avidin-biotin-peroxidase complexes. Specific binding of antibodies to cartilaginous structures was seen after injection of 20-500 micrograms biotinylated monoclonal or polyclonal anti-type II collagen antibodies, but not after injection of a biotinylated control antibody. This technique should further the detection and localization studies of tissue components involved in the dynamics of physiological and pathological processes.

Experimental Study on Construction of Tissue Engineered Bone by Autologous Oxygen Release Nano-Bionic Scaffold Combined with Bone Morphogenetic Protein-2 Induced Bone Marrow Mesenchymal Stem Cells

2019 ◽  
Vol 9 (9) ◽  
pp. 1254-1260
Author(s):  
Fei Wang ◽  
Hongfang Wei ◽  
Chengdong Hu ◽  
Dongfeng Li ◽  
Xiwei Huo ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Type Ii Collagen ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Type Ii ◽  
Oxygen Release ◽  
Fracture Group ◽  
Bionic Scaffold ◽  
Marrow Mesenchymal Stem Cells

Bone marrow mesenchymal stem cells (BMSCs) are used for bone tissue engineering. BMP-2 and autologous oxygen-releasing nano-bionic scaffolds promote bone differentiation of BMSCs. Our study intends to evaluate the role of autologous oxygen-releasing nano-bionic scaffolds combined with BMP-2-induced BMSCs in the construction of tissue engineered bone. Rat BMSCs were isolated and transfected with NC (negative control group) and BMP-2 (BMP-2 plasmid group), respectively. Healthy male SD rats were randomly and equally divided into fracture group, negative control group and the BMP-2 group which was implanted with autologous oxygen-releasing nano-bionic scaffolds to synthesize BMSCs and transfected with BMP-2 plasmids respectively followed by analysis of osteophytes growth, ALP activity, expression of BMP-2, type II collagen, Runx2 and OC by real time PCR, TGF-β1 secretion by ELISA and BMP-2 protein expression by western blot. BMSCs induced by autologous oxygen release nano-bionic scaffold combined with BMP-2 can significantly promote the increase of bone mineral density, increase the expression of Runx2 and OC, promote ALP activity, upregulate type II collagen, BMP-2 mRNA and protein, and TGF-β1 secretion compared to fracture group (P < 0.05). The BMSCs induced by autologous oxygen-releasing nanobionic scaffolds transfected with BMP-2 had a more significant effect on bone repair. Autologous oxygen-releasing nano-bionic scaffolds combined with BMP-2-induced BMSCs can promote bone healing by regulating BMP-2 and increasing osteogenesis at the bone defect.

BIOCHEMICAL PREDICTORS OF CARTILAGINOUS TISSUE METABOLIC DISORDERS FOR EARLY OSTEOARTHROSIS EVIDENCE DIAGNOSTICS

2020 ◽  
Vol 65 (3) ◽  
pp. 155-162
Author(s):  
E. V. Gladkova
Keyword(s):  
Metabolic Disorders ◽  
Renal Excretion ◽  
Comparison Group ◽  
Hyaline Cartilage ◽  
Type Ii Collagen ◽  
Control Group ◽  
Type Ii ◽  
X Ray ◽  
Radiological Methods ◽  
Early Oa

The complete laboratory and clinical instrumental examination was conducted, it included serum COMP test, circadian excretion of type II collagen C-terminal telopeptides Urine CartiLaps (СТХ II) and Т2 relaxometry in 29 patients of both sexes of the main group with early (0-I) X-ray osteoarthrosis stages, 30 subjects of comparison group with no X-ray osteoarthrosis evidences aged 44.7±5.9 years and 25 healthy subjects aged 26.3±2.6 years of the control group. The increase (р<0,05) of COMP and Urine CartiLaps levels as well as the increase of Т2 relaxation signal was found at early osteoarthrosis evidences. It was proven that there was (р<0.01) a connection (R=0.8) between COMP and Urine CTX II levels as well as (р<0.05) results of Т2 relaxometry (R=0.8). It was proven that collagen anisotropy and formation of chondromalacia areas as Т2 relaxometry showed in patients with early OA evidences were connected with accumulation of serum COMP and increase of type II collagen circadian renal excretion. The combination of laboratory and radiological methods of articular hyaline cartilage assessment may be used for finding early osteoarthrosis stages.

scholarly journals Effects of Twin Inclined Plane Device on Adaptation and Ultrastructure Variations in Condyle of Growing Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Shuo Wang ◽  
Yuhong Sun ◽  
Lulu Xia ◽  
Hanyue Li ◽  
Yan Xu ◽  
...  
Keyword(s):  
Subchondral Bone ◽  
Type Ii Collagen ◽  
Ultrastructural Changes ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Type Ii ◽  
Inclined Plane ◽  
Growing Rats ◽  
Osteoclastic Activity ◽  
Experimental Group

Objective. This study investigates the effects of using a twin inclined plane device (TIPD) on the remolding and ultrastructure variation of mandibular condyle in growing rats. Materials and Methods. Forty-eight male Wistar rats (six weeks old, body weight of approximately 190–210 g) were divided into experimental group (wearing appliance, n = 32) and control group (no appliance, n = 16). Samples were collected on days 3, 14, 30, and 60. The immunohistochemical analysis for vascular endothelial growth factor (VEGF) and type II collagen was carried out. Tartrate-resistant acid phosphatase (TRAP) reaction was performed to evaluate the osteoclastic activity. Three-dimensional morphometric images were reconstructed for morphometric analysis by microcomputed tomography (micro-CT). The ultrastructure of the condylar surface was observed by scanning electron microscopy (SEM). Results. The expression of VEGF significantly increased, while the expression of type II collagen decreased in the experimental group at days 30 and 60. Furthermore, the enhanced osteoclast activity was observed under the subchondral bone, which was highest at day 30, and decreased to the lowest at day 60 in the experimental group. In addition, adaptive subchondral bone remolding in the posterior part of the condyle was observed at day 60 in the experimental group, and the SEM revealed the ultrastructure variations after installation of the TIPD. However, these changes began to reverse after 30 days. Conclusion. Condylar tissue changes point to the osteoclastic activity in the posterior region of the condyle. These adaptive changes point to bone resorption in the posterior condyle. Type II collagen and VEGF contribute to the MCC remolding induced by the TIPD. The ultrastructural changes in the posterior condylar area in response to mechanical stresses are recoverable at the initial stage.

Production of Type II Collagen Specific Monoclonal Antibodies

1994 ◽  
Vol 23 (2) ◽  
pp. 85-98 ◽  
Author(s):  
G. R. Srinivas ◽  
C. O. Chichester ◽  
H.-J. Barrach ◽  
V. Pillai ◽  
A. L. Matoney
Keyword(s):  
Monoclonal Antibodies ◽  
Type Ii Collagen ◽  
Type Ii

scholarly journals Functional Lateral Shift of the Mandible Effects on the Expression of ECM in Rat Temporomandibular Cartilage

2009 ◽  
Vol 79 (4) ◽  
pp. 652-659 ◽  
Author(s):  
Tanapan Wattanachai ◽  
Ikuo Yonemitsu ◽  
Sawa Kaneko ◽  
Kunimichi Soma
Keyword(s):  
Extracellular Matrix ◽  
Experimental Period ◽  
Type Ii Collagen ◽  
Lateral Shift ◽  
Control Group ◽  
Type Ii ◽  
Early Puberty ◽  
Condylar Cartilage ◽  
Cartilage Extracellular Matrix ◽  
Extracellular Matrix Components

Abstract Objective: To test the hypothesis that the effects of mechanical stress from a functional lateral shift of the mandible have no effect on the expression of two main condylar cartilage extracellular matrix components, type II collagen and aggrecan, in rats from early puberty to young adulthood. Materials and Methods: Functional lateral shift of the mandible was induced in experimental groups of 5-week-old male Wistar rats, using guiding appliances. The rats were sacrificed at 3, 7, 14, and 28 days post appliance attachment. The condyles were immunohistochemically evaluated for type II collagen and aggrecan (the immunoreactive areas were quantified). Results: As compared with the control group, on the contralateral condyles, the immunoreactivity of the experimental groups was significantly increased from 7 to 14 days. While on the ipsilateral condyles, the immunoreactive areas were significantly decreased throughout the experimental period. Conclusion: A functional lateral shift of the mandible modulated the condylar cartilage extracellular matrix differently on each side of the condyle, which affected condylar morphology, growth, biomechanical properties, and even the susceptibility of the condylar cartilage to pathogenesis.

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