Angiogenesis Induced by Head and Neck Squamous Cell Carcinoma Xenografts in the Chick Embryo Chorioallantoic Membrane Model

1993 ◽  
Vol 102 (3) ◽  
pp. 215-221 ◽  
Author(s):  
Guy J. Petruzzelli ◽  
Jonas T. Johnson ◽  
Carl H. Snyderman ◽  
Eugene N. Myers
Keyword(s):  
Squamous Cell Carcinoma ◽  
Chick Embryo ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Chorioallantoic Membrane ◽  
Head And Neck Tumors ◽  
Angiogenic Response ◽  
Neck Tumors ◽  
Chick Embryo Chorioallantoic Membrane

The angiogenic potential of head and neck tumors compared to nonneoplastic control tissues was investigated by using the chick embryo chorioallantoic membrane (CAM) as a bioassay for angiogenesis. Eighty tumor specimens from 10 patients with squamous cell carcinoma of the head and neck were grafted onto the CAM of 7-day chick embryos. The presence of tumor in the original explant was confirmed histologically. Forty-four embryos (55%) survived and were evaluated histologically at day 17. Tumors were growing on or invading the CAM mesoderm in 30 of 44 embryos (68%). Before harvesting of the membranes, the tumors and surrounding blood vessels were photographed, and the angiogenic responses were graded by a panel of blinded observers. Tumor explants elicited a significantly greater angiogenic response than nontumor controls (p = .01). We conclude that head and neck squamous cell carcinomas can induce an angiogenic response in vivo, presumably secondary to the production of an unidentified angiogenic factor, and that the chick embryo CAM is an effective model for quantifying angiogenesis induced by head and neck tumors.

Head and neck tumors other than squamous cell carcinoma

2004 ◽  
Vol 16 (3) ◽  
pp. 236-241 ◽  
Author(s):  
Lisa Licitra ◽  
Laura D Locati ◽  
Paolo Bossi ◽  
Giulio Cantù
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Head And Neck Tumors ◽  
Neck Tumors

scholarly journals Effect of Laryngeal Squamous Cell Carcinoma Tissue Implantation on the Chick Embryo Chorioallantoic Membrane: Morphometric Measurements and Vascularity

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Virgilijus Uloza ◽  
Alina Kuzminienė ◽  
Sonata Šalomskaitė-Davalgienė ◽  
Jolita Palubinskienė ◽  
Ingrida Balnytė ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Chick Embryo ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Chorioallantoic Membrane ◽  
Proliferative Capacity ◽  
Tissue Samples ◽  
Chick Embryo Chorioallantoic Membrane ◽  
Cam Model

Background. The aim of this study was to develop chick embryo chorioallantoic membrane (CAM) model of laryngeal squamous cell carcinoma (LSCC) and to evaluate the morphological and morphometric characteristics and angiogenic features of it.Methods. Fresh LSCC tissue samples obtained from 6 patients were implanted onto 15 chick embryo CAMs. Morphological, morphometric, and angiogenic changes in the CAM and chorionic epithelium were evaluated up to 4 days after the tumor implantation. Immunohistochemical analysis (34βE12, CD31, and Ki67 staining) was performed to detect cytokeratins and tumor endothelial cells and to evaluate the proliferative capacity of the tumor before and after implantation on the CAM.Results. The implanted LSCC tissue samples survived on the CAM in all the experiments and retained the essential morphologic characteristics and proliferative capacity of the original tumor. Implants induced thickening of both the CAM (103–417%,p=0.0001) and the chorionic epithelium (70–140%,p=0.0001) and increase in number of blood vessels (75–148%,p=0.0001) in the CAM.Conclusions. This study clarifies that chick embryo CAM is a relevant assay for implanting LSCC tissue and provides the first morphological and morphometric characterization of the LSCC CAM model that opens new perspectives to study this disease.

Dickkopf-1 is a Prognostic Biomarker and Correlated With Immune Infiltrates in Head and Neck Squamous Cell Carcinoma

2021 ◽  
Author(s):  
Chaofen Zhao ◽  
Lina Liu ◽  
Qianyong He ◽  
Yuanyuan Li ◽  
Jieqing Jiang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Immune Cells ◽  
Tumor Infiltrating Lymphocytes ◽  
Neck Squamous Cell Carcinoma ◽  
Normal Tissues ◽  
Neck Tumors ◽  
Dkk1 Expression

Abstract Background: Head and neck squamous cell carcinoma(HNSCC) has a high level of immune infiltration, and immunotherapy generates new hope to patients with head and neck tumors. It is currently one of the most promising treatments for HNSCC. Previous studies have shown that Dickkopf-1 (DKK1), an inhibitor of Wnt signaling, is related to the prognosis of a variety of tumors. DKK1 is a key mediator of immune activation and is highly associated with tumor progress and tumor microenvironment. However, the association between DKK1 expression and the prognosis of HNSCC and tumor infiltrating lymphocytes is still unknown.Objective: We aim to test the relationship between DKK1 and HNSCC prognosis and tumor infiltrating lymphocytes.Methods:We explored the DKK1 expression differences in multiple tumor tissues and normal tissues via examing TIMER, Oncomine, and UALCAN databases. Then, we searched the Kaplan-Meier database to assess the correlation between DKK1 mRNA levels and clinical outcomes. Subsequently, the TIMER platform and TISIDB website were selected to evaluate the correlation between DKK1 and the level of tumor immune cell infiltration. We used cBioPortal and UCSC Xena database to further explore the reasons for the aberrant expression of DKK1 in tumorigenesis.Results: We found that DKK1 level was significantly elevated in HNSCC tissues compared with normal tissues. The expression of DKK1 is related to the tumor stage, tumor grade, histology, lymph node metastasis and worse clinical prognosis of HNSCC. In addition, DKK1 is negatively correlated with different tumor-infiltrating immune cells in HNSCC. Moreover, DKK1 is closely related to the genetic markers of a variety of immune cells. Also, the prognosis of HNSCC is worse based on the high DKK1 expression of immune cells. Copy number variation (CNV) and DNA methylation may be the cause of abnormal up-regulation of DKK1 in HNSCC.Conclusion: DKK1 can be used as a biomarker for predicting the prognosis and immune infiltration of HNSCC. These results provide us with important clues to better understand the role of DKK1 in the immunotherapy of head and neck tumors from the perspective of bioinformatics.This study provides a theoretical basis for clinical research on the treatment of head and neck squamous cell carcinoma with the DKK1 gene as a target. It may play a major role in advancing the immunotherapy of head and neck squamous cell carcinoma and enhance the therapeutic effect.

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