Production of Monoclonal Antibodies against Diquat and Its Enzyme-Linked Immunosorbent Assay
1993 ◽
Vol 33
(2)
◽
pp. 117-120
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Keyword(s):
Monoclonal antibodies(MoAbs) very specific to diquat (DQ) were produced. An immunogen was synthesized by binding DQ to bovine serum albumin via a diazo-coupled derivative. BALB/c mice were immunized i.p. monthly with 0.25mg of the immunogen for five months. Their spleen cells were fused with P3U1 myeloma cells and hybridoma clones secreting MoAbs were obtained. Two MoAbs were selected and subtyped to be IgM and IgG3. The MoAbs recognized DQ but did not bind to paraquat and other analogues at all. A datum obtained from a clinical sample demonstrates that an enzyme-linked immunosorbent assay system using one of the MoAbs is useful in the practice of toxicological analysis.
1996 ◽
Vol 79
(2)
◽
pp. 426-430
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1989 ◽
Vol 17
(2)
◽
pp. 414-414
◽
2004 ◽
Vol 22
(SI - Chem. Reactions in Foods V)
◽
pp. S199-S202
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2020 ◽
1987 ◽
Vol 15
(3)
◽
pp. 245-250
◽
2012 ◽
Vol 384
(1-2)
◽
pp. 148-151
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