Internal Lipid Wool Structure Modification Due to a Nonionic Auxiliary Used in Dyeing at Low Temperatures

1997 ◽  
Vol 67 (2) ◽  
pp. 131-136 ◽  
Author(s):  
L. Coderch ◽  
O. Lopez ◽  
A. de la Maza ◽  
A. M. Manich ◽  
J. L. Parra ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Detection System ◽  
Morphological Structure ◽  
Microscopical Study ◽  
Flame Ionization ◽  
Membrane Complex ◽  
Wool Fibers ◽  
Previously Treated ◽  
Layer Chromatography ◽  
Cell Membrane Complex

An analytical and microscopical study of wool fibers differently treated with a non-ionic dye auxiliary product such as Baylan NT elucidates the action mechanism of this compound in low temperature dyeing of wool. Lipids are extracted from wool samples previously treated with Baylan NT, and the compounds are analyzed quantitatively with thin layer chromatography coupled to a flame ionization detection system. Previous solubilization of free fatty acids by the Baylan treatment is considered to be the main cause of some changes in the lipid morphological structure, resulting in the release of ceramides in subsequent solvent extraction. These are found in a significantly higher amount in the extract of the wool treated with the auxiliary compound. Furthermore, the microscopical study shows higher penetration of the fluorescent dye in wool fibers treated with the auxiliary compound, thereby confirming the importance of cell membrane complex modification in the improving the dyeing characteristics of wool and permitting dyeing at low temperatures.

New Advances in the Internal Lipid Composition of Wool

1988 ◽  
Vol 58 (6) ◽  
pp. 338-342 ◽  
Author(s):  
B. Nogues ◽  
L. Coderch ◽  
R. Julia ◽  
P. Erra
Keyword(s):  
Cell Membrane ◽  
Organic Solvents ◽  
Lipid Composition ◽  
Bilayer Structure ◽  
Membrane Complex ◽  
Wool Fibers ◽  
Cell Membrane Complex

The presence of glycolipids on wool fibers has been detected by analyzing the solubilized material when wool is treated with different organic solvents selected to remove internal lipids. One of these has been isolated and identified as a glucosilceramide. These compounds are thought to contribute to the bilayer structure that may be present in the β-layers of the cell membrane complex.

Role of Nonkeratinous Proteins in Crimp Formation of Wool Fibers by Draft and Immediate Relaxation

1988 ◽  
Vol 58 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Ryo Umehara ◽  
Yutaka Shibata ◽  
Yoshihiko Masuda ◽  
Hiraku Ito ◽  
Takeaki Miyamoto ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Formic Acid ◽  
Relaxation Process ◽  
Proteolytic Enzyme ◽  
Membrane Complex ◽  
Wool Fibers ◽  
Cell Membrane Complex

The role of nonkeratinous proteins in the crimp formation of wool fibers by draft and immediate relaxation was investigated. For this purpose, Australian Shropshire wool fibers were treated with formic acid and pronase (proteolytic enzyme) to modify the nonkeratinous proteins. Formic acid is known to remove some of the intercellular cement, one of the nonkeratinous proteins, from the cell membrane complex, while pronase removes all nonkeratinous proteins. Little crimp formation occurred in the wool fibers treated with formic acid and pronase, indicating that the nonkeratinous proteins, especially the intercellular cement of the cell membrane complex, play an important role in the crimp formation of wool fibers by the draft and immediate relaxation process. A similar effect on crimp formation also occurred when the wool fibers were steamed in a relaxed state.

scholarly journals Function of the Cell Membrane Complex on Dyeing of Wool Fibers with Oxidation Dye

2006 ◽  
Vol 62 (12) ◽  
pp. 280-286 ◽  
Author(s):  
Kyohei Joko ◽  
Yumi Yoshikatsu ◽  
Keiko Sakata
Keyword(s):  
Cell Membrane ◽  
Membrane Complex ◽  
Wool Fibers ◽  
Cell Membrane Complex

Analysis of Wool Lipids Using Thin-Layer Chromatography with Flame Ionization Detection

1988 ◽  
Vol 58 (10) ◽  
pp. 593-600 ◽  
Author(s):  
Ian H. Leaver ◽  
David M. Lewis ◽  
David J. Westmoreland
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Detection System ◽  
Soxhlet Extraction ◽  
Quantitative Detection ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Layer Chromatography

This paper examines the usefulness of thin-layer chromatography (TLC), coupled with an automated quantitative detection system based on flame ionization detection (FID), for the qualitative and quantitative determination of lipids in wool. The latroscan TLC-FID system has been used to determine the composition of the solvent soluble material (internal lipids) isolated from wool after Soxhlet extraction with a chloroform/methanol azeotrope, and to investigate whether scouring treatments affect the composition of the internal lipids. Changes in the composition of wool grease that occur as a result of exposure to sunlight (behind glass) and during weathering in the fleece are also examined.

scholarly journals A validated quantification of triclosan in toothpaste using high-performance thin-layer chromatography and a 48-bit fatbed scanner

2021 ◽  
Author(s):  
Barbara Anders ◽  
Sabrina Doll ◽  
Bernd Spangenberg
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Detection System ◽  
Flatbed Scanner ◽  
Butyl Ether ◽  
Quantification Method ◽  
Direct Measurements ◽  
Layer Chromatography

AbstractWe present a densitometric quantification method for triclosan in toothpaste, separated by high-performance thin-layer chromatography (HPTLC) and using a 48-bit flatbed scanner as the detection system. The sample was band-wise applied to HPTLC plates (10 × 20 cm), with fluorescent dye, Merck, Germany (1.05554). The plates were developed in a vertical developing chamber with 20 min of chamber saturation over 70 mm, using n-heptane–methyl tert-butyl ether–acetic acid (92:8:0.1, V/V) as solvent. The RF value of triclosan is hRF = 22.4, and quantification is based on direct measurements using an inexpensive 48-bit flatbed scanner for color measurements (in red, green, and blue) after plate staining with 2,6-dichloroquinone-4-chloroimide (Gibbs' reagent). Evaluation of the red channel makes the measurements of triclosan very specific. For linearization, an extended Kubelka–Munk expression was used for data transformation. The range of linearity covers more than two orders of magnitude and is between 91 and 1000 ng. The separation method is inexpensive, fast and reliable.

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